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Search Results: 1 - 10 of 311 matches for " Spermatozoa "
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Ergot Alkaloid Effects on Bovine Sperm Motility In Vitro  [PDF]
Ryan Page, Toby Lester, Rick Rorie, Charles Rosenkrans Jr.
Advances in Reproductive Sciences (ARSci) , 2019, DOI: 10.4236/arsci.2019.71002
Abstract: Cattle in some parts of the world graze pastures that consist of tall fescue that may contain ergot alkaloid contamination. Those ergot alkaloids are associated with reduced reproductive rates in cattle. Our objective was to determine if ergot alkaloids [dihydroergotamine (DHET), ergonovine (EN), and ergotamine (ET)] directly affect bovine sperm characteristics. Spermatozoa were collected from mature Angus (n = 2) and Balancer (n = 4) bulls. Within bull, treatments were structured as a 3 × 5 factorial with three alkaloids (DHET, EN, and ET) and five concentrations of each alkaloid (0, 33, 66, 100, or 200 μM). Spermatozoa (25 × 106) were incubated (39?C) in 1 mL of modified sperm medium. Sperm motility characteristics were evaluated using CASA (Hamiliton Thorne IVOS, Beverly, MA) at 0, 3, and 6 h after initial alkaloid exposure. Initial sperm motility was (69% ± 1.1%) and declined (P = 0.01) to (35% ± 2.6%) at 6 h. Sperm motility decreased (P < 0.05) with increasing concentrations of DHET and ET, but not EN. As concentration of ET or DHET increased all CASA sperm characteristics were altered. The interaction of alkaloid concentration and incubation length affected sperm velocity and head size; exposure to 200 μM of ET or DHET for six hours decreased (P < 0.05) both characteristics. Our results demonstrate that ergot alkaloids (ET and DHET) can directly alter bovine sperm motility and morphology, which adds to our understanding of how ergot alkaloids may hinder cattle reproductive rates.
Motility and Functional State of the Membrane of Caprine Capacitated Spermatozoa under Different Chemical Agents  [PDF]
A. Soberano Martínez, J. Herrera Camacho, J. C. Segura Correa
Open Journal of Veterinary Medicine (OJVM) , 2012, DOI: 10.4236/ojvm.2012.23017
Abstract: The objective of this study was to test the use of a commercial extender (Triladyl) as a diluent in caprine semen refrigerated at 15℃, using caffeine (CF), heparin (HP), synthetic oviductal fluid (SOF) andtriladyl (TRY) as capacitating chemical agents at different times. Twenty ejaculates of caprine semen were collected using an artificial vagina. The ejaculates were diluted and refrigerated by three days. Evaluated the progressive motility (PM) and the functional state of the sperm plasma membrane trough fluorescent CTC staining, counting 200 spermatic cells (non-capacitated spermatozoa NCS, capacitated CS and reacting spermatozoa REA) of caprine in two capacitating agents: CAF and HEP; one culture medium:SOF and a commercial extender: TRY at 60, 120, 180 and 240 min of incubation, during 24, 48 and 72 hs. PM was high under TRY, and CS was high under the HEP treatment. TRY could be an alternative to capacitate caprine spermatozoa, keeping PM for a longer time than HEP or CAF.
Relación entre variables subjetivas e informatizadas del movimiento espermático del morueco
Bravo,J.A.; Montanero,J.; Calero,R.; Roy,T.J.;
Archivos de Zootecnia , 2011,
Abstract: the present study examines the existing relationships among sperm motility variables in ejaculates from ?le de france rams throughout a year, obtained by means of both subjective analysis and computer-assisted system isas? (integrated semen analysis system). the results show a strong association among parameters of the same kind, being the correlation coefficient r>0.80 (p<0.01) when the relationship occurs between linear velocity (vsl) and average path velocity (vap), between porcentage of inmovil spermatozoa (est) and rapid espermatozoa (rap), and between masal motility (mm) and individual motitity (mi). the relationships among groups of a different kind (velocities vs. variables expressed as percentages) are moderate or low, when existent; however, those between lineatity (lin) and lateral head displacement (alh) (r= -0.866, p<0.01) and between mi and rap (r= 0.799, p<0.01) are high. in view of the results, it may be stated that there exists a concordance between the parameters subjectively established and those provided by computer-assisted system casa, being mi the subjective parameter that best explains the variables obtained by isas?.
Bambang Wasito,Sarwanto Sarwanto
Bulletin of Health Research , 2012,
Intraflagellar Protein 88 Interactome Analysis: A Bioinformatics Approach Highlights Its Role in Testis and Sperm Function  [PDF]
Maria Jo?o Freitas, Margarida Fardilha
Journal of Biophysical Chemistry (JBPC) , 2014, DOI: 10.4236/jbpc.2014.53011
Intraflagellar transport (IFT) is essential for cilium and flagellar assembly. This movement is accomplished by two IFT complexes: A and B. IFT88, intraflagellar protein 88, is a core element of IFT complex B. This protein has been linked to migration, to olfactory function, spindle formation and to mitosis. Recently, IFT88 was identified as a TCTEX1D4 interacting protein in human testis, suggesting a role in male reproduction. To broaden the knowledge on IFT88 function, particularly in testis and spermatozoa, an in silico analysis of IFT88 and IFT88 interactome was undertaken. IFT88 appears to be prone to protein-protein interactions, involved in spermatogenesis and since it interacts with key proteins related to male fertility, it may have a role in reproduction.
Variation among Beef Bulls in the Ratio of X- to Y-Chromosome Bearing Spermatozoa  [PDF]
Rick W. Rorie, Pedro A. Delgado, Toby D. Lester
Advances in Reproductive Sciences (ARSci) , 2014, DOI: 10.4236/arsci.2014.24008
Abstract: A study was conducted to evaluate variation in the ratio of X- to Y-bearing sperm of individual ejaculates and to determine any relationship between skewed sex ratio and either routine morphological evaluation or computer assisted sperm analysis (CASA). Semen was collected from bulls weekly for 6 consecutive weeks, sperm DNA was recovered and quantitative, and real-time PCR was used to determine the ratio of X- to Y-bearing sperm in each ejaculate. The overall mean of X-bearing sperm within ejaculates was 54.7% over the 6 weeks of semen collections. The percentages of X-bearing sperm were similar (P > 0.5) across all collections. Between bulls, there were differences (P < 0.05) in the mean percentage of X-bearing sperm. No significant correlations were found between CASA parameters and percentage of X-bearing sperm across bulls, so analysis was done within each bull. Different combinations of CASA and/or morphological parameters were found to correlate with the percentage of X-bearing sperm but the prediction equations were specific for individual bulls and unlikely to be of use across bulls. These results confirm that the ratio of X- to Y-bearing sperm may be skewed in some ejaculates of bull semen. Some sperm parameters measured by CASA or routine morphological evaluation were associated with semen sex ratio.
Colloid Centrifugation of Semen: Applications in Assisted Reproduction  [PDF]
Jane M. Morrell, Heriberto Rodriguez-Martinez
American Journal of Analytical Chemistry (AJAC) , 2016, DOI: 10.4236/ajac.2016.78055
Abstract: Colloid centrifugation can be used to separate heterogeneous populations of cells particularly semen samples, which typically contain mixtures of spermatozoa at different stage of maturity together with dead and dying spermatozoa and also non-sperm cells. Colloids can be used to enrich the population of spermatozoa that are viable and functional; these are the spermatozoa that are needed for fertilization in Assisted Reproduction. Sperm samples obtained after colloid centrifugation may be enriched for motile, morphologically normal spermatozoa with intact chromatin. Insemination of mares with stallion sperm samples prepared by colloid centrifugation resulted in a higher pregnancy rate per cycle than controls, confirming that the good sperm attributes observed in the laboratory were indicative of functional spermatozoa. The method does not simply enrich for viable spermatozoa by inhibiting dead or dying spermatozoa from passing through the colloid since emerging evidence shows that colloid centrifugation can select for spermatozoa with certain properties such as metabolic activity. Other reproductive cells, such as spermatids or progenitor cells, may also be purified by colloid centrifugation. This review describes the history of colloid centrifugation for selecting spermatozoa, compares colloid centrifugation with alternative techniques, and finally describes some of the applications of the technique within the animal breeding industry.
Evaluation of Steroid Receptors mRNA Fingerprints in Two Groups of Normozoospermic Patients: Men from Unexplained Infertility Couples vs. Men from Couples with Tubal Factor Infertility  [PDF]
Katarzyna Jarzabek, Agnieszka Mikucka-Niczyporuk, Tomasz Bielawski, Robert Milewski, Jacek Z. Kubiak, Slawomir Wolczynski
Open Journal of Obstetrics and Gynecology (OJOG) , 2017, DOI: 10.4236/ojog.2017.73031
Abstract: The study of sperm cellular components at molecular level is crucial for the diagnosis of male unexplained infertility. The aim of the study was to compare the molecular profile of steroid receptors and aromatase in spermatozoa obtained from two normozoospermic groups of patients issued from couples treated for infertility. We investigated 46 male patients from unexplained infertility couples and from men, 38 where female partners presented with tubal infertility. Sperm ERs (estrogen receptors: alpha and beta), GPER (G protein-coupled estrogen receptor), AR (androgen receptor) and aromatase mRNA expression levels by TaqMan qPCR were analyzed. AR transcript level was significantly lower in sperm of men from unexplained infertility couples vs. men from couples with tubal factor infertility (P = 0.04). Although the AR mRNA expression level did not had any effect on embryo development and its implantation, a significant correlation between AR mRNA levels and clinical pregnancy in unexplained infertility patients was observed. Taken together, AR transcript presence in ejaculated spermatozoa could be a potential marker for unexplained infertility.
Effect of Storage Time on Cauda Epididymal Sperm Parameters of Nigerian Local Dogs  [PDF]
Uchenna Macnissi Chima, Adakole Hyacinth Abu, Philip Makama Dawuda, Aboh Iku Kisani, Terzungwe Ahemen
Open Journal of Veterinary Medicine (OJVM) , 2017, DOI: 10.4236/ojvm.2017.711016
Abstract: Aim: Storage of testes-epididymides at refrigeration temperature is a promising method of short-term sperm preservation for use in reproductive biotechnology in dogs. This study aimed to determine the effect of storage time at 4°C on the quality of Nigerian local dogs’ cauda epididymal spermatozoa recovered post-castration. Materials and Methods: Testes along with their epididymides collected immediately after castration of local dogs were either processed immediately (0th hour) or stored at 4°C in the refrigerator for 12th, 24th or 48th hours and then processed. Each storage group comprised 5 pairs of testes-epididymides. Sperm motility, concentration, livability, acrosome integrity and morphology from the various storage groups were evaluated and means with standard error of the mean (±SEM) were recorded. Results: Mean percent sperm motility at 0th hour (77.0 ± 2.6) decreased significantly (p < 0.05) after 12 hours (68.0 ± 1.2), 24 hours (66.0 ± 2.4) and 48 hours (60.0 ± 1.6) of storage at 4°C. No significant difference (p > 0.05) was found when the mean sperm concentrations (×107/ml) of the storage groups (5.86 ± 47, 5.24 ± 26 and 5.38 ± 31) were compared with the control (6.48 ± 0.75). Mean percent sperm livability did not differ significantly (p > 0.05) between the 0th, 12th, 24th and 48th hour groups (86.4 ± 1.5, 78.0 ± 3.1 and 75.0 ± 1.8 respectively). However, there was a significant decrease (p < 0.05) in mean percent sperm livability after 48 hours of storage (69.8 ± 4.2) as compared with the 0th hour group. No significant difference (p > 0.05) was detected in the mean percent acrosome integrity between the 0th, 12th, 24th and 48th hour groups (90.0 ± 1.5, 83.0 ± 3.5, 84.6 ± 4.6 and 82.2 ± 3.9 respectively). A significant increase (p < 0.05) in percent sperm morphological defect was detected after 48 hours of storage (37.6 ± 2.7) as compared to the 0th hour group (27.4 ± 2.0). Conclusion: The current study has shown that viable spermatozoa can be recovered after 48 hours of storage at 4°C of the cauda epididymides of Nigerian local dogs.
Sperm ultrastructure in three different families of weakly electric fishes (Teleostei: Gymnotiformes)
Giora, Júlia;Burns, John R.;
Neotropical Ichthyology , 2011, DOI: 10.1590/S1679-62252011005000047
Abstract: this study presents details of sperm ultrastructure for gymnotus aff. carapo (gymnotidae), eigenmannia trilineata (sternopygidae), and three brachyhypopomus species (b. draco, b. bombilla, and b. gauderio - hypopomidae) from southern brazil. differences were found among the representatives of the different families. for example, nuclear rotation was present in e. trilineata and in the brachyhypopomus species, but absent in gymnotus aff. carapo, and the presence of flagellar fins was only observed in e. trilineata. some intraspecific variations could also be noticed among the brachyhypopomus species analyzed. most of the characters found in the spermatozoa of the species studied herein are shared with species of gymnotiformes previously analyzed.
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