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Search Results: 1 - 10 of 561011 matches for " Souza M.R. "
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Atividade antimicrobiana de bactérias lácticas isoladas de queijo-de-minas artesanal do Serro (MG) frente a microrganismos indicadores
Alexandre, D.P.;Silva, M.R.;Souza, M.R.;Santos, W.L.M.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002, DOI: 10.1590/S0102-09352002000400014
Abstract: a total of 192 strains of lactic acid bacteria were isolated from five samples of artisanal minas cheese, from serro region - minas gerais state. the results of direct inhibition test indicated that 48 strains (25%) inhibited the in vitro growth of the indicator microorganisms: staphylococcusaureus and listeriamonocytogenes. those 48 strains were tested by the indirect inhibition test, and 10 strains (20.8%) showed a wide antimicrobial spectrum against salmonellaenteritidisvar. typhimurium, staphylococcusaureus, listeria monocytogenes and listeria innocua. none of the isolated strains was able to inhibit escherichia coli.
Atividade antimicrobiana de bactérias lácticas isoladas de queijo-de-minas artesanal do Serro (MG) frente a microrganismos indicadores
Alexandre D.P.,Silva M.R.,Souza M.R.,Santos W.L.M.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2002,
Abstract: Isolaram-se 192 cepas de bactérias lácticas de cinco amostras de queijo-de-minas artesanal, oriundos da regi o do Serro, MG. Os resultados da prova de inibi o direta indicaram que 48 cepas isoladas (25%) foram capazes de inibir o crescimento in vitro de microrganismos indicadores, dentre os quais Staphylococcus aureus e Listeria monocytogenes. As 48 cepas foram submetidas à prova de inibi o indireta. Verificou-se que 20,8% (10/48) das cepas demonstraram amplo espectro antimicrobiano frente a microrganismos como: Salmonella enteritidis var. typhimurium, Staphylococcus aureus, Listeria monocytogenes e Listeria innocua. Nenhuma das cepas isoladas foi capaz de inibir Escherichia coli.
Efeito da chuva na eficiência de herbicidas aplicados em pós-emergência sobre corda-de-viola Effect of rainfall on the efficiency of herbicides applied in post-emergence on Ipomoea grandifolia
G.S.F Souza,D Martins,M.R.R Pereira
Planta Daninha , 2013, DOI: 10.1590/s0100-83582013000100019
Abstract: O objetivo deste trabalho foi avaliar a influência da chuva na eficiência de herbicidas aplicados em pós-emergência em plantas de Ipomoea grandifolia. As plantas de I. grandifolia foram cultivadas em vasos plásticos com capacidade de 2,5 L, em casa de vegeta o, com uma planta por vaso. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repeti es, com os tratamentos dispostos em um esquema fatorial 7x8 (sete tratamentos químicos e oito períodos para ocorrência de chuva após a aplica o dos tratamentos). Os tratamentos químicos constaram da aplica o de glyphosate em cinco formula es comerciais (Roundup Original, Roundup WG, Roundup Transorb, Roundup Transorb R e Roundup Ultra) a 1.080 g e.a. ha-1, am nio-glufosinate (Finale) a 400 g i.a. ha-1 e 2,4-D (DMA 806) a 1.000 g e.a. ha-1 e de oito intervalos de tempo para simula o de uma chuva de 15 mm, com dura o de cinco minutos: 15', 30', 1h, 2h, 4h, 6h, 8h após a aplica o dos tratamentos e uma testemunha sem chuva, Foram realizadas avalia es visuais de controle das plantas aos 7, 14, 21, 28 e 35 dias após a aplica o e, por ocasi o da última avalia o, determinou-se a massa seca das plantas. A ocorrência de chuvas após a aplica o de 2,4-D n o alterou a sua eficiência no controle das plantas de I. grandifolia; já os herbicidas am nio-glufosinate e glyphosate, em todas suas formula es testadas, apresentaram redu o na eficiência de controle quando da ocorrência de chuvas em até oito horas após a aplica o dos tratamentos. The objective of this study was to evaluate the influence of rainfall on the efficiency of herbicides applied in post-emergence on Ipomoea grandifolia plants. I. grandifolia plants were sown in plastic pots with a capacity of 2.5L under greenhouse conditions, with only one plant per pot. The experiment was arranged in a completely randomized design, with four replications, with the treatments arranged in a 7 x 8 factorial (seven chemical treatments and eight rainfall periods after treatment application). Chemical treatments consisted of application of glyphosate in five commercial formulations (Roundup Original, Roundup WG, Roundup Transorb, Roundup Transorb R, and Roundup Ultra) to 1,080ga.e. ha-1, glufosinate-ammonium (Finale) to 400ga.i. ha-1 and 2,4-D (DMA 806) and 1,000ga.e. ha-1, and eight time intervals to simulate 15mm rain, lasting five minutes (15', 30', 1h, 2h , 4h, 6h, and 8h) after application of the treatments and a control without rain. Visual evaluations of plant control were carried out at 7, 14, 21, 28, and 35 days after application and
Comercializa??o de plantas medicinais no contexto da cadeia produtiva em Minas Gerais
Souza, M.R.M.;Pereira, R.G.F.;Fonseca, M.C.M.;
Revista Brasileira de Plantas Medicinais , 2012, DOI: 10.1590/S1516-05722012000500019
Abstract: the national politic integrative and complementary practices in health system (sus) contributed to the increased use and knowledge of brazilian medicinal plants, which encourages and supports the growing of family farmers and their integration in efficient markets. the program for the development of medicinal plants prioritizes actions in the entire production chain from cultivation to distribution. knowledge of demand and adequate quality standards allows distributors and consumers to make decisions on the acquisition and supply of medicinal products. however, there is a lack of information and technologies in this supply chain, which discourages its full development, consistent with the potential for income generation and health promotion presents. the objective of this study was to identify and analyze current patterns of production and marketing of medicinal plants in the central market of belo horizonte. we conducted semi-structured interviews with nine of the 23 stores trades of medicinal plants from the central market. results showed the precarious nature of this sector in obtaining medicinal plants, mainly by lack of knowledge and information in the purchase and sale of products by consumers and sellers, respectively.
Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning
Vianna M.R.M.,Izquierdo L.A.,Barros D.M.,de Souza M.M.
Brazilian Journal of Medical and Biological Research , 2001,
Abstract: Rats implanted bilaterally with cannulae in the CA1 region of the dorsal hippocampus or the entorhinal cortex were submitted to either a one-trial inhibitory avoidance task, or to 5 min of habituation to an open field. Immediately after training, they received intrahippocampal or intraentorhinal 0.5-μl infusions of saline, of a vehicle (2% dimethylsulfoxide in saline), of the glutamatergic N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphono pentanoic acid (AP5), of the protein kinase A inhibitor Rp-cAMPs (0.5 μg/side), of the calcium-calmodulin protein kinase II inhibitor KN-62, of the dopaminergic D1 antagonist SCH23390, or of the mitogen-activated protein kinase kinase inhibitor PD098059. Animals were tested in each task 24 h after training. Intrahippocampal KN-62 was amnestic for habituation; none of the other treatments had any effect on the retention of this task. In contrast, all of them strongly affected memory of the avoidance task. Intrahippocampal Rp-cAMPs, KN-62 and AP5, and intraentorhinal Rp-cAMPs, KN-62, PD098059 and SCH23390 caused retrograde amnesia. In view of the known actions of the treatments used, the present findings point to important biochemical differences in memory consolidation processes of the two tasks.
Effect of antibodies to intercellular adhesion molecule type 1 on the protection of distant organs during reperfusion syndrome in rats
Souza-Moraes M.R.,David-Filho R.,Baptista-Silva J.C.C.,Ullian M.
Brazilian Journal of Medical and Biological Research , 2003,
Abstract: We investigated kidney and lung alterations caused by intercellular adhesion molecule type 1 (ICAM-1) blockade after ischemia and reperfusion of hind limb skeletal muscles. Rats were submitted to ligature of the infrarenal aorta for 6 h. The animals were randomized into three groups of 6 rats each: group I, sacrificed after ischemia; group II, reperfusion for 24 h, and group III, reperfusion for 24 h after receiving monoclonal anti-ICAM-1 antibodies. At the end of the experiment, blood samples were collected for creatinine, lactate dehydrogenase, creatine phosphokinase, potassium, pH and leukocyte counts. Samples were taken from the muscles of the hind limbs and from the kidneys and lungs for histological analysis and measurement of the neutrophil infiltrate by myeloperoxidase staining. The groups did not differ significantly with regard to the laboratory tests. There were no major histological alterations in the kidneys. An intense neutrophil infiltrate in the lungs, similar in all groups, was detected. Myeloperoxidase determination showed that after reperfusion there was significantly less retention of polymorphonuclear neutrophils in the muscles (352 ± 70 vs 1451 ± 235 × 102 neutrophils/mg; P<0.01) and in the kidneys (526 ± 89 vs 852 ± 73 × 102 neutrophils/mg; P<0.01) of the animals that received anti-ICAM-1 before perfusion compared to the group that did not. The use of anti-ICAM-1 antibodies in this experimental model minimized neutrophil influx, thus reducing the inflammatory process, in the muscles and kidneys after ischemia and reperfusion of the hind limbs.
Eclosion rate, development and survivorship of Aedes albopictus (Skuse)(Diptera: Culicidae) under different water temperatures
Monteiro, Laura C.C.;Souza, José R.B. de;Albuquerque, Cleide M.R. de;
Neotropical Entomology , 2007, DOI: 10.1590/S1519-566X2007000600021
Abstract: in tropical areas, where vector insects populations are particularly numerous, temperature usually range between 25°c and 35°c. considering the importance of such temperature variation in determining mosquitoes population dynamics, in this work the developmental, eclosion and survival rates of the immature stages of aedes albopictus (skuse) were compared under constant 25, 30 and 35°c (using acclimatized chambers) and environmental (25°c to 29°c) temperatures. the hatching rate was considered as total number of larvae recovered after 24h. the development period as well as larval and pupal survival rate were evaluated daily. eclosion rate was significantly higher under environmental temperature than under the studied constant temperatures, suggesting that temperature variation may be an eclosion-stimulating factor. the mean eclosion time increased with the temperature, ranging from 2.8h (25°c) to 5.2h (35°c). the larval period was greatly variable inside each group, although it did not differ significantly amongst groups (11.0 ± 4.19 days), with individuals showing longer larval stages in water at 35°c (12.0 ± 4.95 days) and environmental temperature (13.6 ± 5.98 days). oppositely, survival was strongly affected by the higher temperature, where only one individual lived through to adult phase. the results suggest that population of ae. albopictus from recife may be adapting to increasing of environmental temperatures and that the limiting temperature to larval development is around 35°c.
A??o da chuva sobre a eficiência de Glyphosate no controle de Eichhornia crassipes e Pistia stratiotes
Souza, G.S.F.;Campos, C.F.;Martins, D.;Pereira, M.R.R.;
Planta Daninha , 2011, DOI: 10.1590/S0100-83582011000100007
Abstract: two experiments were carried out to evaluate the effect of rain at different periods of time after glyphosate application, one with eichhornia crassipes and the other with pistia stratiotes, both arranged in a randomized design with four replications. the treatments consisted of simulating a 12 mm rainfall for five minutes, at different periods of time (0h, 2h, 4h, 6h, 12h, 24h, and no rain simulation) after glyphosate application at a dose of 2,160 g a.e. ha-1 (4.5 l c.p. ha-1 rodeo formulation) + 0.5% v/v aterbane. visual evaluations of weed control at 7, 14, 21, and 28 days after application (daa) were conducted. the increase in time without rainfall after glyphosate application provided an excellent control for the two species studied and the treatment without rainfall. the occurrence of rainfall after two hours of glyphosate application effectively controlled e. crassipes plants after 14 daa and p. stratiotes after only 21 daa. treatments with rainfall immediately after herbicide application showed a good control for the two species of aquatic weeds only at 28 daa.
Influência da chuva na eficiência de diquat no controle de Salvinia auriculata, Pistia stratiotes e Eichhornia crassipes
Souza, G.S.F;Campos, C.F;Pereira, M.R.R;Martins, D;
Planta Daninha , 2011, DOI: 10.1590/S0100-83582011000400023
Abstract: the aim of this study was to evaluate the influence of rainfall events at different time periods after application of diquat on the efficiency of this herbicide in controlling salvinia auriculata, pistia stratiotes and eichhornia crassipes. simulation of 10 mm rainfall applied during 5 min was studied at different time periods (0h, 0.25h, 0.5h, 1h, 2h, 4h, 6h, 12h and no rain simulation) after application of diquat in reward formulation, 600 g a.i. ha-1 (2.5lcp ha-1). the experiment was arranged in a completely randomized design with four replications. plants were evaluated at 3, 5, 7, and 12 days after application (daa), and the results submitted to analysis of variance by the f test and the treatment means compared by the tukey test (p < 0.05). diquat provided excellent control of all the species studied, regardless of the time period for rain occurrence after application, even when immediately after application.
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians
Souza D.R.S.,De Godoy M.R.,Hotta J.,Tajara E.H.
Brazilian Journal of Medical and Biological Research , 2003,
Abstract: The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the epsilon4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE epsilon2 frequency was considerably lower in the Alzheimer's disease group (1%), and the epsilon3 allele and epsilon3/epsilon3 genotype frequencies were higher in the controls (84 and 72%, respectively) as were the epsilon4 allele and epsilon3/epsilon4 genotype frequencies in Alzheimer's disease (25 and 41%, respectively). The higher frequency of the epsilon4 allele in Alzheimer's disease confirmed its role as a risk factor, while epsilon2 provided a weak protection against development of the disease. However, in view of the unexpectedly low frequency of the epsilon4 allele, additional analyses in a more varied Brazilian sample are needed to clarify the real contribution of apolipoprotein E to the development of Alzheimer's disease in this population.
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