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Search Results: 1 - 10 of 4659 matches for " Sonia Coni "
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Cellular Trafficking of Thymosin Beta-4 in HEPG2 Cells Following Serum Starvation
Giuseppina Pichiri, Pierpaolo Coni, Sonia Nemolato, Tiziana Cabras, Mattia Umberto Fanari, Alice Sanna, Eliana Di Felice, Irene Messana, Massimo Castagnola, Gavino Faa
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067999
Abstract: Thymosin beta-4 (Tβ4) is an ubiquitous multi-functional regenerative peptide, related to many critical biological processes, with a dynamic and flexible conformation which may influence its functions and its subcellular distribution. For these reasons, the intracellular localization and trafficking of Tβ4 is still not completely defined and is still under investigation in in vivo as well as in vitro studies. In the current study we used HepG2 cells, a human hepatoma cell line; cells growing in normal conditions with fetal bovine serum expressed high levels of Tβ4, restricted to the cytoplasm until 72 h. At 84 h, a diffuse Tβ4 cytoplasmic immunostaining shifted to a focal perinuclear and nuclear reactivity. In the absence of serum, nuclear reactivity was localized in small granules, evenly dispersed throughout the entire nuclear envelop, and was observed as earlier as at 48 h. Cytoplasmic immunostaining for Tβ4 in HepG2 cells under starvation appeared significantly lower at 48 h and decreased progressively at 72 and at 84 h. At these time points, the decrease in cytoplasmic staining was associated with a progressive increase in nuclear reactivity, suggesting a possible translocation of the peptide from the cytoplasm to the nuclear membrane. The normal immunocytochemical pattern was restored when culture cells submitted to starvation for 84 h received a new complete medium for 48 h. Mass spectrometry analysis, performed on the nuclear and cytosolic fractions of HepG2 growing with and without serum, showed that Tβ4 was detectable only in the cytosolic and not in the intranuclear fraction. These data suggest that Tβ4 is able to translocate from different cytoplasmic domains to the nuclear membrane and back, based on different stress conditions within the cell. The punctuate pattern of nuclear Tβ4 immunostaining associated with Tβ4 absence in the nucleoplasm suggest that this peptide might be localized in the nuclear pores, where it could regulate the pore permeability.
Gli2 Acetylation at Lysine 757 Regulates Hedgehog-Dependent Transcriptional Output by Preventing Its Promoter Occupancy
Sonia Coni, Laura Antonucci, Davide D'Amico, Laura Di Magno, Paola Infante, Enrico De Smaele, Giuseppe Giannini, Lucia Di Marcotullio, Isabella Screpanti, Alberto Gulino, Gianluca Canettieri
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0065718
Abstract: The morphogenic Hedgehog (Hh) signaling regulates postnatal cerebellar development and its aberrant activation leads to medulloblastoma. The transcription factors Gli1 and Gli2 are the activators of Hh pathway and their function is finely controlled by different covalent modifications, such as phosphorylation and ubiquitination. We show here that Gli2 is endogenously acetylated and that this modification represents a key regulatory step for Hedgehog signaling. The histone acetyltransferase (HAT) coactivator p300, but not other HATs, acetylates Gli2 at the conserved lysine K757 thus inhibiting Hh target gene expression. By generating a specific anti acetyl-Gli2(Lys757) antisera we demonstrated that Gli2 acetylation is readily detectable at endogenous levels and is attenuated by Hh agonists. Moreover, Gli2 K757R mutant activity is higher than wild type Gli2 and is no longer enhanced by Hh agonists, indicating that acetylation represents an additional level of control for signal dependent activation. Consistently, in sections of developing mouse cerebella Gli2 acetylation correlates with the activation status of Hedgehog signaling. Mechanistically, acetylation at K757 prevents Gli2 entry into chromatin. Together, these data illustrate a novel mechanism of regulation of the Hh signaling whereby, in concert with Gli1, Gli2 acetylation functions as a key transcriptional checkpoint in the control of morphogen-dependent processes.
El Museo de la Mujer y un edifico emblemático
Tejero Coni,Graciela;
La aljaba , 2009,
Abstract: in order to constitute a specific museological archive and reservoir for the cultural history of women, the promotion and the art production, a group of women, professionals and feminists started activities to foster the museo de la mujer. we want to harvest the heritage of the women who make history side by side with their people with the certainty that the totality of the cumulative property is not either a neutral nor fixed cultural capital but a dialectical one, and meaningful from the gender perspective. the aim of museo de la mujer is to exhibit the cultural reality signed by the inequality of gender relationships together with its historical origin, while reflecting at the same time the development of its scientific analysis. for to fulfil yours objectives, the women's museum has requested to grant it the site of the exwomen's jail located on humberto primo no 378 of the city of buenos aires in which to undertake its activities. we feel that it is a significant location, whose history will allow women to identify themselves within those walls.
El Museo de la Mujer y un edifico emblemático The women's museum and the emblematic building
Graciela Tejero Coni
La Aljaba (Luján) , 2009,
Abstract: Con el propósito de constituir un archivo y reservorio museológico específico de la historia cultural de las mujeres, la promoción y producción del arte, un grupo de mujeres, profesionales y feministas, iniciamos las actividades de fomento del Museo de la Mujer. Para recoger la herencia de las mujeres que hacen la historia junto al pueblo, con la convicción de que el conjunto de los bienes acumulables no son un capital cultural neutro ni fijo, sino dialéctico y con significante desde la perspectiva de género. La misión del Museo de la Mujer es exhibir la realidad cultural signada por la desigualdad de las relaciones de género y su origen histórico. Así mismo reflejar la evolución de su análisis científico. Para cumplir sus fines, el Museo de la Mujer ha solicitado la cesión del edificio de la "ex - Cárcel de Mujeres", sito en Humberto Primo 378 de la Ciudad Autónoma de Buenos Aires, para desarrollar sus actividades. Entendiendo que es un lugar con significado, que por su propia historia, propone una atmósfera concreta que invita a todas las mujeres a mirarse y reconocerse dentro de esas paredes. In order to constitute a specific museological archive and reservoir for the cultural history of women, the promotion and the art production, a group of women, professionals and feminists started activities to foster the Museo de la Mujer. We want to harvest the heritage of the women who make history side by side with their people with the certainty that the totality of the cumulative property is not either a neutral nor fixed cultural capital but a dialectical one, and meaningful from the gender perspective. The aim of Museo de la Mujer is to exhibit the cultural reality signed by the inequality of gender relationships together with its historical origin, while reflecting at the same time the development of its scientific analysis. For to fulfil yours objectives, the Women's Museum has requested to grant it the site of the exWomen's Jail located on Humberto Primo No 378 of the City of Buenos Aires in which to undertake its activities. We feel that it is a significant location, whose history will allow women to identify themselves within those walls.
THE RIGHT TO EDUCATION ACT: AN OVERVIEW
SONIA
Indian Streams Research Journal , 2012,
Abstract: A man without education is no more than an animal. The growth and development of a state is primarily dependent upon the quality of education given to the people. In India Right to Education Act (RTE) was passed on 4th August 2009 which provides for free and compulsory education for children between the age of 6 and 14 years. The Act came into force on 1 April 2010. The problems on the path of education of children includes child labour, misery of girl child in India and poor quality of education. Article 51A of constitution also makes its obligatory for parent or guardian as the case may be to provide opportunities for education to his child or ward between the age of six and fourteen years. Although RTE Act is a major landmark in the history of India but there are certain limitations of the Act which if removed then education will reach every child in India which is their right. “There is only one purpose in the whole of Life –Education”.“Otherwise what is the use of me and women, land and wealth”. – Swami Vivekananda“Education is the most powerful weapon which you can use to change the world”.– Nelson Mandela
Bioethical Questions in Brazilian Scientific Research  [PDF]
Sonia Vieira
International Journal of Clinical Medicine (IJCM) , 2014, DOI: 10.4236/ijcm.2014.516130
Abstract:

It is considered here that the ethical behavior of the Brazilian scientific community leaves a lot to be desired. Motives for ethical lapses can be summed up by one only: the haste to grow at any price in order to earn prestige and wealth, setting at naught those who don’t have the political support of the institution. With this intent, many researchers publish whatever they can, neglecting the fact that scientific research should serve the interests of the community—not their own.

Early Childhood Education: Difficulties Creating and Changing Daily Practice  [PDF]
Sonia Kramer
Creative Education (CE) , 2014, DOI: 10.4236/ce.2014.56048
Abstract:

This paper is based on research carried out with children and adults at early childhood education institutions in Rio de Janeiro, Brazil. The research goals were to identify interactions between adults and children and to rethink pedagogical practice. The theoretical-methodological framework was based on language and culture studies, the sociology of childhood and anthropology. After studying children and adults in different contexts and early childhood education public policies and practice, the results present challenges of changing and creating early education practices in difficult contexts, especially with regard to the creation and use of spaces intended for children. This is the focus of the text, which is structured in three sections. Section one presents the theoretical and methodological issues. The second section analyzes three case studies developed in public pre-schools situated in different cities in the state of Rio de Janeiro. Section three presents conclusions and priorities for intervention and change concerning children, adults and their interactions.

The Onset of Schizophrenia in Adolescence: Developments from a Structural and Clinical Point of View  [PDF]
Sonia Alberti
Open Journal of Medical Psychology (OJMP) , 2014, DOI: 10.4236/ojmp.2014.32014
Abstract: In 1899, Kraepelin identified schizophrenia as early dementia. The precocity of the onset of schizophrenia may be verified in psychotic episodes in the clinic of adolescence, which this article explores both from the point of view of psychoanalytic theory, and from the point of view of its clinic, in particular regarding the transference of the psychotic adolescent. It departs from the importance of differential diagnosis in relation to neurosis, using the guidelines of Freud about the loss of reality, then studies the contributions of Lacan in relation to that which returns from the real when not included in the symbolic. Finally, it articulates the drive theory to identify the issue of jouissance in transference.
Toward to the Journey’s End  [PDF]
Sonia Vieira
Open Journal of Philosophy (OJPP) , 2016, DOI: 10.4236/ojpp.2016.64029
Abstract: People become aware of the human life finiteness when they reach middle age. If they are healthy, they believe they will reach old age with white hair. However, in old age, the awareness that the process of dying is slowly adding losses over losses will come. Therefore, we should cultivate dignity in life because we do not know how we will face death: probably with suffering without respectability, as we never imagine it.
Research on Chewing Gum: Questions of Ethics  [PDF]
Sonia Vieira
Open Journal of Philosophy (OJPP) , 2017, DOI: 10.4236/ojpp.2017.73016
Abstract: Guidelines for clinical research and ethics committees are significant contributions to decision made in science and law. But since research methods need to follow the changes and the development of science, guidelines for clinical research have to be continuously revised. Many innovations are perceived for their intended beneficiaries as benefits. Unfortunately, this is not always true. Therefore two issues should be mandatory in discussions of research ethics committees: 1) What constitutes a harmful intervention? 2) What procedures should be established for obtaining data and evaluating their relevance? These questions are too broad and there are no ethical solutions that can leap off a paper. But an example can be worked on. Dental caries is a public health challenge in any country and chewing gum may have an effect with respect to plaque reduction, at least in a short period of time. But sugar chewing gum cannot be used as a positive control in biomedical researches. However, trials referred herein allocated participants for a sugar chewing gum group. Therefore, the aim of this critical review is to emphasize that such a procedure is an example of a conscious disregard for the consideration which is due to trial participants. Were these desirable researches themselves or they had other purposes?
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