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Search Results: 1 - 10 of 542285 matches for " Sogayar M.C. "
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Functional analysis of newly discovered growth control genes: experimental approaches
Flatschart, R.B.;Sogayar, M.C.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000700011
Abstract: a large number of dna sequences corresponding to human and animal transcripts have been filed in data banks, as cdnas or ests (expression sequence tags). however, the actual function of their corresponding gene products is still largely unknown. several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. the currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cdna screening by functional analysis are discussed.
Functional analysis of newly discovered growth control genes: experimental approaches
Flatschart R.B.,Sogayar M.C.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed.
Oncogene-mediated downregulation of RECK, a novel transformation suppressor gene
Sasahara, R.M.;Takahashi, C.;Sogayar, M.C.;Noda, M.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000700014
Abstract: the reck gene was initially isolated as a transformation suppressor gene encoding a novel membrane-anchored glycoprotein and later found to suppress tumor invasion and metastasis by regulating matrix metalloproteinase-9. its expression is ubiquitous in normal tissues, but undetectable in many tumor cell lines and in fibroblastic lines transformed by various oncogenes. the reck gene promoter has been cloned and characterized. one of the elements responsible for the oncogene-mediated downregulation of mouse reck gene is the sp1 site, where the sp1 and sp3 factors bind. sp1 transcription factor family is involved in the basal level of promoter activity of many genes, as well as in dynamic regulation of gene expression; in a majority of cases as a positive regulator, or, as exemplified by the oncogene-mediated suppression of reck gene expression, as a negative transcription regulator. the molecular mechanisms of the downregulation of mouse reck gene and other tumor suppressor genes are just beginning to be uncovered. understanding the regulation of these genes may help to develop strategies to restore their expression in tumor cells and, hence, suppress the cells' malignant behavior.
Oncogene-mediated downregulation of RECK, a novel transformation suppressor gene
Sasahara R.M.,Takahashi C.,Sogayar M.C.,Noda M.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: The RECK gene was initially isolated as a transformation suppressor gene encoding a novel membrane-anchored glycoprotein and later found to suppress tumor invasion and metastasis by regulating matrix metalloproteinase-9. Its expression is ubiquitous in normal tissues, but undetectable in many tumor cell lines and in fibroblastic lines transformed by various oncogenes. The RECK gene promoter has been cloned and characterized. One of the elements responsible for the oncogene-mediated downregulation of mouse RECK gene is the Sp1 site, where the Sp1 and Sp3 factors bind. Sp1 transcription factor family is involved in the basal level of promoter activity of many genes, as well as in dynamic regulation of gene expression; in a majority of cases as a positive regulator, or, as exemplified by the oncogene-mediated suppression of RECK gene expression, as a negative transcription regulator. The molecular mechanisms of the downregulation of mouse RECK gene and other tumor suppressor genes are just beginning to be uncovered. Understanding the regulation of these genes may help to develop strategies to restore their expression in tumor cells and, hence, suppress the cells' malignant behavior.
Hunting for differentially expressed genes
Vedoy, C.G.;Bengtson, M.H.;Sogayar, M.C.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000700012
Abstract: differentially expressed genes are usually identified by comparing steady-state mrna concentrations. several methods have been used for this purpose, including differential hybridization, cdna subtraction, differential display and, more recently, dna chips. subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.
Hunting for differentially expressed genes
Vedoy C.G.,Bengtson M.H.,Sogayar M.C.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: Differentially expressed genes are usually identified by comparing steady-state mRNA concentrations. Several methods have been used for this purpose, including differential hybridization, cDNA subtraction, differential display and, more recently, DNA chips. Subtractive hybridization has significantly improved after the polymerase chain reaction was incorporated into the original method and many new protocols have been established. Recently, the availability of the well-known coding sequences for some organisms has greatly facilitated gene expression analysis using high-density microarrays. Here, we describe some of these modifications and discuss the benefits and drawbacks of the various methods corresponding to the main advances in this field.
Mechanisms of cell transformation induced by polyomavirus
Oliveira, M.L.S.;Brochado, S.M.;Sogayar, M.C.;
Brazilian Journal of Medical and Biological Research , 1999, DOI: 10.1590/S0100-879X1999000700010
Abstract: polyomavirus is a dna tumor virus that induces a variety of tumors in mice. its genome encodes three proteins, namely large t (lt), middle t (mt), and small t (st) antigens, that have been implicated in cell transformation and tumorigenesis. lt is associated with cell immortalization, whereas mt plays an essential role in cell transformation by binding to and activating several cytoplasmic proteins that participate in growth factor-induced mitogenic signal transduction to the nucleus. the use of different mt mutants has led to the identification of mt-binding proteins as well as analysis of their importance during cell transformation. studying the molecular mechanisms of cell transformation by mt has contributed to a better understanding of cell cycle regulation and growth control.
Mechanisms of cell transformation induced by polyomavirus
Oliveira M.L.S.,Brochado S.M.,Sogayar M.C.
Brazilian Journal of Medical and Biological Research , 1999,
Abstract: Polyomavirus is a DNA tumor virus that induces a variety of tumors in mice. Its genome encodes three proteins, namely large T (LT), middle T (MT), and small T (ST) antigens, that have been implicated in cell transformation and tumorigenesis. LT is associated with cell immortalization, whereas MT plays an essential role in cell transformation by binding to and activating several cytoplasmic proteins that participate in growth factor-induced mitogenic signal transduction to the nucleus. The use of different MT mutants has led to the identification of MT-binding proteins as well as analysis of their importance during cell transformation. Studying the molecular mechanisms of cell transformation by MT has contributed to a better understanding of cell cycle regulation and growth control.
Bone morphogenetic proteins: from structure to clinical use
Granjeiro, J.M.;Oliveira, R.C.;Bustos-Valenzuela, J.C.;Sogayar, M.C.;Taga, R.;
Brazilian Journal of Medical and Biological Research , 2005, DOI: 10.1590/S0100-879X2005001000003
Abstract: bone morphogenetic proteins (bmps) are multi-functional growth factors belonging to the transforming growth factor ? superfamily. family members are expressed during limb development, endochondral ossification, early fracture, and cartilage repair. the activity of bmps was first identified in the 1960s but the proteins responsible for bone induction were unknown until the purification and cloning of human bmps in the 1980s. to date, about 15 bmp family members have been identified and characterized. the signal triggered by bmps is transduced through serine/threonine kinase receptors, type i and ii subtypes. three type i receptors have been shown to bind bmp ligands, namely: type ia and ib bmp receptors and type ia activin receptors. bmps seem to be involved in the regulation of cell proliferation, survival, differentiation and apoptosis, but their hallmark is their ability to induce bone, cartilage, ligament, and tendon formation at both heterotopic and orthotopic sites. this suggests that, in the future, they may play a major role in the treatment of bone diseases. several animal studies have illustrated the potential of bmps to enhance spinal fusion, repair critical-size defects, accelerate union, and heal articular cartilage lesions. difficulties in producing and purifying bmps from bone tissue have prompted the attempts made by several laboratories, including ours, to express these proteins in the recombinant form in heterologous systems. this review focuses on bmp structure, molecular mechanisms of action and significance and potential applications in medical, dental and veterinary practice for the treatment of cartilage and bone-related diseases.
Bone morphogenetic proteins: from structure to clinical use
Granjeiro J.M.,Oliveira R.C.,Bustos-Valenzuela J.C.,Sogayar M.C.
Brazilian Journal of Medical and Biological Research , 2005,
Abstract: Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor superfamily. Family members are expressed during limb development, endochondral ossification, early fracture, and cartilage repair. The activity of BMPs was first identified in the 1960s but the proteins responsible for bone induction were unknown until the purification and cloning of human BMPs in the 1980s. To date, about 15 BMP family members have been identified and characterized. The signal triggered by BMPs is transduced through serine/threonine kinase receptors, type I and II subtypes. Three type I receptors have been shown to bind BMP ligands, namely: type IA and IB BMP receptors and type IA activin receptors. BMPs seem to be involved in the regulation of cell proliferation, survival, differentiation and apoptosis, but their hallmark is their ability to induce bone, cartilage, ligament, and tendon formation at both heterotopic and orthotopic sites. This suggests that, in the future, they may play a major role in the treatment of bone diseases. Several animal studies have illustrated the potential of BMPs to enhance spinal fusion, repair critical-size defects, accelerate union, and heal articular cartilage lesions. Difficulties in producing and purifying BMPs from bone tissue have prompted the attempts made by several laboratories, including ours, to express these proteins in the recombinant form in heterologous systems. This review focuses on BMP structure, molecular mechanisms of action and significance and potential applications in medical, dental and veterinary practice for the treatment of cartilage and bone-related diseases.
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