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Search Results: 1 - 10 of 35 matches for " Sjef Boeren "
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Isolation and Identification of Glycinol from Glycine max [L.] Merri.
大豆中Glycinol的分离与鉴定

QI Yongyan,MOCO Sofia,BOEREN Sjef,de VOS C H Ric,BOVY Arnaud,
乞永艳
,MOCO Sofia,BOEREN Sjef,de VOS C H Ric,BOVY Arnaud

色谱 , 2005,
Abstract: As one of the main phytoalexins and phytoestrogens, glyceollin is an important prenylflavonoid in Glycine max L.] Merri. (soybean). Many kinds of elicitors can be used to induce its accumulation. Its biosynthesis pathway is commonly used to study the characteristics of prenyltransferase, which catalyzes the prenylated reaction happening in a very few plant families in nature. Glycinol, the direct precursor of glyceollin, is necessary to study the prenylated reaction in soybean. In comparing with the other elicitors to elicit the glycinol accumulation in soybean cotyledons, AgNO3 is the most effective elicitor. Exposure of 6-8 days old cotyledons to 0.01 mol/L AgNO3 and incubation for 24 h result in the accumulation of 256 microg (glycinol)/g (fresh weight). The glycinol was extracted by methanol. Then the isolation and purification were conducted by preparative high performance liquid chromatography. Instead of 100% acetonitrile-0.1% formic acid as the elution system, the extract was eluted by 100% methanol-0.1% formic acid. Glycinol eluted earlier than daidzin under this system and decreased the disturbance from the large amount of daidzin. Identification was performed by comparing the mass spectrum (liquid chromatography/quadrupole-time of flight) and ultraviolet spectrum with those of the standard. At last, 100 mg purified glycinol was obtained from 390 g of fresh material.
The Host Defense Proteome of Human and Bovine Milk
Kasper Hettinga,Hein van Valenberg,Sacco de Vries,Sjef Boeren,Toon van Hooijdonk,Johan van Arendonk,Jacques Vervoort
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019433
Abstract: Milk is the single source of nutrients for the newborn mammal. The composition of milk of different mammals has been adapted during evolution of the species to fulfill the needs of the offspring. Milk not only provides nutrients, but it also serves as a medium for transfer of host defense components to the offspring. The host defense proteins in the milk of different mammalian species are expected to reveal signatures of evolution. The aim of this study is therefore to study the difference in the host defense proteome of human and bovine milk. We analyzed human and bovine milk using a shot-gun proteomics approach focusing on host defense-related proteins. In total, 268 proteins in human milk and 269 proteins in bovine milk were identified. Of these, 44 from human milk and 51 from bovine milk are related to the host defense system. Of these proteins, 33 were found in both species but with significantly different quantities. High concentrations of proteins involved in the mucosal immune system, immunoglobulin A, CD14, lactoferrin, and lysozyme, were present in human milk. The human newborn is known to be deficient for at least two of these proteins (immunoglobulin A and CD14). On the other hand, antimicrobial proteins (5 cathelicidins and lactoperoxidase) were abundant in bovine milk. The high concentration of lactoperoxidase is probably linked to the high amount of thiocyanate in the plant-based diet of cows. This first detailed analysis of host defense proteins in human and bovine milk is an important step in understanding the function of milk in the development of the immune system of these two mammals.
The Salivary Secretome of the Tsetse Fly Glossina pallidipes (Diptera: Glossinidae) Infected by Salivary Gland Hypertrophy Virus
Henry M. Kariithi,Ikbal A. Ince,Sjef Boeren,Adly M. M. Abd-Alla,Andrew G. Parker,Serap Aksoy,Just M. Vlak ,Monique M. van Oers
PLOS Neglected Tropical Diseases , 2011, DOI: 10.1371/journal.pntd.0001371
Abstract: Background The competence of the tsetse fly Glossina pallidipes (Diptera; Glossinidae) to acquire salivary gland hypertrophy virus (SGHV), to support virus replication and successfully transmit the virus depends on complex interactions between Glossina and SGHV macromolecules. Critical requisites to SGHV transmission are its replication and secretion of mature virions into the fly's salivary gland (SG) lumen. However, secretion of host proteins is of equal importance for successful transmission and requires cataloging of G. pallidipes secretome proteins from hypertrophied and non-hypertrophied SGs. Methodology/Principal Findings After electrophoretic profiling and in-gel trypsin digestion, saliva proteins were analyzed by nano-LC-MS/MS. MaxQuant/Andromeda search of the MS data against the non-redundant (nr) GenBank database and a G. morsitans morsitans SG EST database, yielded a total of 521 hits, 31 of which were SGHV-encoded. On a false discovery rate limit of 1% and detection threshold of least 2 unique peptides per protein, the analysis resulted in 292 Glossina and 25 SGHV MS-supported proteins. When annotated by the Blast2GO suite, at least one gene ontology (GO) term could be assigned to 89.9% (285/317) of the detected proteins. Five (~1.8%) Glossina and three (~12%) SGHV proteins remained without a predicted function after blast searches against the nr database. Sixty-five of the 292 detected Glossina proteins contained an N-terminal signal/secretion peptide sequence. Eight of the SGHV proteins were predicted to be non-structural (NS), and fourteen are known structural (VP) proteins. Conclusions/Significance SGHV alters the protein expression pattern in Glossina. The G. pallidipes SG secretome encompasses a spectrum of proteins that may be required during the SGHV infection cycle. These detected proteins have putative interactions with at least 21 of the 25 SGHV-encoded proteins. Our findings opens venues for developing novel SGHV mitigation strategies to block SGHV infections in tsetse production facilities such as using SGHV-specific antibodies and phage display-selected gut epithelia-binding peptides.
An iterative workflow for mining the human intestinal metaproteome
Koos Rooijers, Carolin Kolmeder, Catherine Juste, Jo?l Doré, Mark de Been, Sjef Boeren, Pilar Galan, Christian Beauvallet, Willem M de Vos, Peter J Schaap
BMC Genomics , 2011, DOI: 10.1186/1471-2164-12-6
Abstract: Two human fecal samples for which metagenomic data had been collected, were analyzed for their metaproteome using liquid chromatography-mass spectrometry and used to benchmark the developed iterative workflow to other methods. The results show that the developed method is able to detect over 3,000 peptides per fecal sample from the spectral data by circumventing the lack of a defined proteome without naive translation of matched metagenomes and cross-species peptide identification.The developed iterative workflow achieved an approximate two-fold increase in the amount of identified spectra at a false discovery rate of 1% and can be applied in metaproteomic studies of the human intestinal tract or other complex ecosystems.The human intestinal tract is colonized since birth by a large number of microbes, together making a complex ecosystem, even considered an organ by itself [1]. Many studies indicate a pivotal role for the intestinal microbes in carbohydrate metabolism, production of vitamins, inflammatory response regulation, fat metabolism and other biological processes of the human host [2,3]. In adults, the community consists of around 1014 cells [4-6], with a complexity that is predicted to include over 5000 microbial species [3]. While recent progress has been made in characterizing the genomes of around 200 intestinal species in the Human Microbiome Project (HMP) [7], the vast majority has not yet been cultured. Hence, these are known as phylotypes as their presence can be deduced from molecular markers such as 16S rRNA and other nucleotide sequences. This approach has shown that most of the intestinal phylotypes belong to a limited set of phyla, including the Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria and Verrucomicrobia [5]. In healthy adults the intestinal microbiota fluctuates around a stable individual core of phylotypes that are affected by host genetics, environmental and stochastic factors [3]. High throughput metagenomics studies, such
Identification and localization of the structural proteins of anguillid herpesvirus 1
Steven J van Beurden, Baptiste Leroy, Ruddy Wattiez, Olga LM Haenen, Sjef Boeren, Jacques JM Vervoort, Ben PH Peeters, Peter JM Rottier, Marc Y Engelsma, Alain F Vanderplasschen
Veterinary Research , 2011, DOI: 10.1186/1297-9716-42-105
Abstract: The Alloherpesviridae family, belonging to the Herpesvirales order comprises all bony fish and amphibian herpesviruses [1]. Currently, the family contains 4 genera with 11 species [2]. At least another 17 herpesviruses infecting bony fish have been described, but have not yet been sufficiently characterized to allow classification [1,3,4]. Many of these viruses cause serious disease and mortality in their respective host species, many of which are important aquaculture species. For example, channel catfish virus or Ictalurid herpesvirus 1 (IcHV-1) may cause up to 100% mortality in channel catfish (Ictalurus punctatus) fingerlings, which posed a significant problem in the big catfish aquaculture industry in the United States [5]. Koi herpesvirus or Cyprinid herpesvirus 3 (CyHV-3) is another highly contagious and virulent disease in its host species common carp and koi (Cyprinus carpio spp.), the first being one of the most economically valuable aquaculture species worldwide [6,7].The eel herpesvirus anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in the European eel, Anguilla anguilla, with increased mortality rates [8]. Because of its omnipresence in wild Western European eel stocks, AngHV-1 is regarded as one of the possible factors responsible for the decline of the wild European eel stocks since the 1980s [9]. Although the fundamental characteristics of herpesviruses of especially humans and mammals have been studied intensively, there is still little knowledge on the herpesviruses of lower vertebrates and invertebrates.Despite their diversity in genes, host range and genome size, the virion structure is conserved throughout the entire Herpesvirales order [1]. Herpesvirus virions invariably consist of a large (diameter >100 nm) icosahedral nucleocapsid (T = 16) containing the genome, surrounded by a host-derived envelope with a diameter of about 200 nm, and an intervening proteinaceous layer called the tegument [10]. For a better understanding of t
Proteomics Analysis of the Zebrafish Skeletal Extracellular Matrix
Maurijn Y. Kessels, Leonie F. A. Huitema, Sjef Boeren, Sander Kranenbarg, Stefan Schulte-Merker, Johan L. van Leeuwen, Sacco C. de Vries
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090568
Abstract: The extracellular matrix of the immature and mature skeleton is key to the development and function of the skeletal system. Notwithstanding its importance, it has been technically challenging to obtain a comprehensive picture of the changes in skeletal composition throughout the development of bone and cartilage. In this study, we analyzed the extracellular protein composition of the zebrafish skeleton using a mass spectrometry-based approach, resulting in the identification of 262 extracellular proteins, including most of the bone and cartilage specific proteins previously reported in mammalian species. By comparing these extracellular proteins at larval, juvenile, and adult developmental stages, 123 proteins were found that differed significantly in abundance during development. Proteins with a reported function in bone formation increased in abundance during zebrafish development, while analysis of the cartilage matrix revealed major compositional changes during development. The protein list includes ligands and inhibitors of various signaling pathways implicated in skeletogenesis such as the Int/Wingless as well as the insulin-like growth factor signaling pathways. This first proteomic analysis of zebrafish skeletal development reveals that the zebrafish skeleton is comparable with the skeleton of other vertebrate species including mammals. In addition, our study reveals 6 novel proteins that have never been related to vertebrate skeletogenesis and shows a surprisingly large number of differences in the cartilage and bone proteome between the head, axis and caudal fin regions. Our study provides the first systematic assessment of bone and cartilage protein composition in an entire vertebrate at different stages of development.
ISABELLE ET MARGUERITE OU LES PLIURES DU FéMININ
Sjef Houppermans
Revue Electronique de Litterature Francaise : RELIEF , 2008,
Abstract: Isabelle de Charrière (1740-1805) montre dans ses romans épistolaires, pour le contenu comme pour la forme et le style, une préoccupation constante avec la séparation, l’absence et le manque. Ici les Lettres écrites de Lausanne vont nous retenir plus spécifiquement suivant cette perspective. Marguerite Yourcenar (1903-1987) trace partout dans son uvre les lignes d’un désir qui ne cesse de s’épuiser dans ses pertes et ses failles. C’est plus particulièrement la nouvelle Anna, Soror, histoire d’inceste sur fond de décor baroque, qui exhibe les traces de l’étrangeté familière colorant l’élan fictionnel. Les deux écrivaines se croisent dans un semblable liage scriptural.
A L’ENVERS, à L’ENDROIT : Freud et le littéraire, à la source A L’ENVERS, à L’ENDROIT : Freud et le littéraire, à la source
Sjef Houppermans
Revue Electronique de Litterature Francaise : RELIEF , 2010,
Abstract: Dans une lettre à Wilhelm Fliess datant de 1898 Freud donne pour la toute première fois le commentaire d’un texte littéraire. Il souligne l’analogie entre la nouvelle de Conrad Ferdinand Meyer, Die Richterin, et le roman familial de ses patients. On peut suggérer que la lecture de Meyer par Freud aura aussi ses répercussions sur l’évolution ultérieure de la théorie psychanalytique. Dans une lettre à Wilhelm Fliess datant de 1898 Freud donne pour la toute première fois le commentaire d’un texte littéraire. Il souligne l’analogie entre la nouvelle de Conrad Ferdinand Meyer, Die Richterin, et le roman familial de ses patients. On peut suggérer que la lecture de Meyer par Freud aura aussi ses répercussions sur l’évolution ultérieure de la théorie psychanalytique.
à LA RECHERCHE DES IMAGES PERDUES : Proust et Heuet
Sjef Houppermans
Revue Electronique de Litterature Francaise : RELIEF , 2008,
Abstract: Les adaptations en bd des grands chefs-d’ uvre de la littérature constituent une catégorie à part. Stéphane Heuet s’est astreint à ‘stripper’ La recherche de Marcel Proust. Nous examinerons dans quelle mesure on peut parler de fidélité à l’original et, d’autre part, de quelle manière l’auteur a su exploiter les moyens spécifiques de son média.
Tanguy Viel : de la parole a l’image Tanguy Viel : de la parole a l’image
Sjef Houppermans
Revue Electronique de Litterature Francaise : RELIEF , 2012,
Abstract: Dans l’oeuvre de Tanguy Viel c’est surtout le roman Insoup onnable de 2006 que nous examinerons pour sa portée mélancolique où argent, désir et conditions sociales s’amalgament sous des regards filtrant le manque d’être. Ensuite la fresque biblique que développe le triptyque Péplum / Personal Jesus / Cet homme-là né sous le signe de Giotto sera contemplé de travers. Dans l’oeuvre de Tanguy Viel c’est surtout le roman Insoup onnable de 2006 que nous examinerons pour sa portée mélancolique où argent, désir et conditions sociales s’amalgament sous des regards filtrant le manque d’être. Ensuite la fresque biblique que développe le triptyque Péplum / Personal Jesus / Cet homme-là né sous le signe de Giotto sera contemplé de travers.
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