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Search Results: 1 - 3 of 3 matches for " Sirisak Visessenee "
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Automatic Training Data Synthesis for Handwriting Recognition Using the Structural Crossing-Over Technique
Sirisak Visessenee,Sanparith Marukatat,Rachada Kongkachandra
Computer Science , 2014,
Abstract: The paper presents a novel technique called "Structural Crossing-Over" to synthesize qualified data for training machine learning-based handwriting recognition. The proposed technique can provide a greater variety of patterns of training data than the existing approaches such as elastic distortion and tangent-based affine transformation. A couple of training characters are chosen, then they are analyzed by their similar and different structures, and finally are crossed over to generate the new characters. The experiments are set to compare the performances of tangent-based affine transformation and the proposed approach in terms of the variety of generated characters and percent of recognition errors. The standard MNIST corpus including 60,000 training characters and 10,000 test characters is employed in the experiments. The proposed technique uses 1,000 characters to synthesize 60,000 characters, and then uses these data to train and test the benchmark handwriting recognition system that exploits Histogram of Gradient (HOG) as features and Support Vector Machine (SVM) as recognizer. The experimental result yields 8.06% of errors. It significantly outperforms the tangent-based affine transformation and the original MNIST training data, which are 11.74% and 16.55%, respectively.
The performance of rotary power tiller using prototype rotary blades in dry-land field
Sirisak Chertkiattipol
Maejo International Journal of Science and Technology , 2008,
Abstract: The effect of shape of prototype rotary blades on the performance of rotary power tiller was investigated in this study. Three sets of rotors, i.e. 14-blade rotor of the Japanese C-shape blade (4.5 cm tilling width of one blade; T1), 14-blade rotor of the prototype rotary blade no. 1 (4.5 cm tilling width of one blade; T2), and 10-blade rotor of the prototype rotary blade no. 2 (6.5 cm tilling width of one blade; T3) were used. The tests were conducted in a dry-land field of clay loam with soil moisture content of 16.04 % (d.b.) and dry bulk density of 1.51 g/cm3 at different rotational speeds of 300, 350 and 400 rpm at one and two tilling passes. For all rotors, experimental results showed that the mean soil clod diameter decreased and soil inversion increased with increasing rotational speed of the rotor. The mean soil clod diameter decreased at pass 2. Soil inversion during pass 2 was higher than pass 1. However, the three sets of rotors showed no significant difference on mean soil clod diameter and soil inversion. The shape of blade prototype rotary blade no. 1 and the decreasing number of prototype rotary blade no. 2 did not affect the tillage performance as compared with the Japanese C-shaped blade.
Disulfide Linkages in Plasmodium falciparum Plasmepsin-I Are Essential Elements for Its Processing Activity and Multi-Milligram Recombinant Production Yield
Sirisak Lolupiman, Pilaiwan Siripurkpong, Jirundon Yuvaniyama
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089424
Abstract: Plasmodium falciparum plasmepsin-I (PM-I) has been considered a potential drug target for the parasite that causes fatal malaria in human. Determination of PM-I structures for rational design of its inhibitors is hindered by the difficulty in obtaining large quantity of soluble enzyme. Nearly all attempts for its heterologous expression in Escherichia coli result in the production of insoluble proteins in both semi-pro-PM-I and its truncated form, and thus require protein refolding. Moreover, the yields of purified, soluble PM-I from all reported studies are very limited. Exclusion of truncated semi-pro-PM-I expression in E. coli C41(DE3) is herein reported. We also show that the low preparation yield of purified semi-pro-PM-I with autoprocessing ability is mainly a result of structural instability of the refolded enzyme in acidic conditions due to incomplete formation of disulfide linkages. Upon formation of at least one of the two natural disulfide bonds, nearly all of the refolded semi-pro-PM-I could be activated to its mature form. A significantly improved yield of 10 mg of semi-pro-PM-I per liter of culture, which resulted in 6–8 mg of the mature PM-I, was routinely obtained using this strategy.
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