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Search Results: 1 - 10 of 474313 matches for " Silvia Denise Pe?a Betancourt "
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Microbiota and Mycotoxins in Trilinear Hybrid Maize Produced in Natural Environments at Central Region in Mexico  [PDF]
Pea Betancourt, Silvia Denise
Advances in Microbiology (AiM) , 2016, DOI: 10.4236/aim.2016.69066
Abstract: Mycotoxigenic fungi and mycotoxins in 3 inbred lines (hybrids resistant to corn ear rot) were identified in twenty samples. The maize (Zea mays) accessions were collected in five plots of two municipalities in High Valley, state of Hidalgo. The fungal population was determined with a microbiological dilution method used two culture media (PDA and ELA), for the detection of mycotoxins with thin layer chromatography with visual inspection in UV light and a direct competitive enzyme-linked immunosorbent (ELISA). The results showed high moisture content in all hybrids evaluated on an average of 38.3% and a 1.8 × 103 UFC/g fungus, values within the permitted limits by the Mexican legislation; however the most prevalent fungi were Fusarium sp. (76%), Alternaria sp. (14%), Penicillium sp. (4%) and Aspergillus sp. (5%), and the species Aspergillus nidulas, Aspergillus flavus, Fusarium verticillioides, Fusarium poae, and Penicillium ochraceum. The aflatoxin concentration was observed in a range from 2 to 13 ng/g and 370 to 660 ng/g to fumonisins. It is concluded that trilinear corn hybrids have a variety of pathogenic potential fungi. The two genetic hybrids showed levels of aflatoxins and fumonisin safe for human consumption, contrary to one hybrid, with a content not suitable for human consumption. A better understanding of genetic hybrids corn will improve predictive mycotoxin contamination.
Recombinant protein detection and its content in total protein, lipids and toxic antinutritional substances in Mexican maize  [PDF]
Pea Betancourt Silvia Denise, Posadas Manzano Eduardo, Valladares Carranza Benjamín
Health (Health) , 2013, DOI: 10.4236/health.2013.510A1002
Abstract:

The engineering genetic technology has developed Bt maize events which contain recombinant protein that will be safe for the consumer. The aflatoxins are contaminants present in maize capable of producing cancer and decreasing the immune response in human, additionally contained polyphenols compounds considered non nutritive. The objective of this study was to identify the presence of recombinant protein in hybrid and local varieties of corn and evaluate the content of aflatoxins and tannins. 25 samples of white grain maize for human consumption were collected, 12 were for hybrid maize and 13 local varieties, from the states of Hidalgo, Mexico and Morelos. Samples were analyzed for Cry1Ab/Cry1Ac, using lateral flow strip method, crude protein and lipids by standard methods. Aflatoxins were assessed by comercial Elisa kit and tannins by spectroscopy method. The data were grouped in a completely random model and an analysis of variance was performed. The results indicated that 44.5% of hybrid corn was positive by Bt-Cry1Ab/1Ac proteins, containing 9.02% ± 2.5 lipids and 11.33% ± 2.2 crude protein, 189 ± 0.92 mg/g of tannins and 6.36 ± 3.3 μg·g-1 aflatoxins. The local maize samples (55.5%) were negative to Bt-Cry1Ab/ Cry1Ac, which protein content was of 8.68% ± 0.90, 6.14% lipids ± 2.3, 273 ± 0.40 mg/100g tannin and 7.15 ± 3.3 μg·g-1 of aflatoxins. In conclusion, we observed an improvement of nutrient composition in hybrid maize with Bt proteins, and decrease in tannins content comparing with some local varieties without Bt proteins. The

Estimation of Mycotoxin Multiple Contamination in Mexican Hybrid Seed Maize by HPLC-MS/MS  [PDF]
Silvia Denise Pea Betancourt, Benjamín Valladares Carranza, Eduardo Posadas Manzano
Agricultural Sciences (AS) , 2015, DOI: 10.4236/as.2015.69104
Abstract: In Mexico, the presence of mycotoxins in chemical treated seed maize by sowing is not well known, despite the need to improve the quality and corn safe for human consumption. It collected twenty-five genotypes maize samples from Morelos State in the spring of 2013, all of them treated with synthetic colors (pink, green, yellow), fungicides and insecticides. Two samples (synthetic seed and hybrid commercial) were selected for analysis of twenty-two mycotoxins by LC-MS/MS and AFB1 determination by liquid chromatography and fluorescence detection (HPLC-FLD). The results of the 25 samples showed the presence of Aflatoxin B1 in 25% of samples in a ranged concentration between 2 to 6 μg·kg-1, and average of 4.1-1 μg·kg, which were within the allowed limits by national and international legislation. Twenty-two mycotoxins were found in levels ranging between 791.7 and 891.2 μg·kg. The content average in both samples was for total aflatoxins (AFB1, AFB2, AFG1, AG2) of 16.95 μg·kg, with G aflatoxins the most prevalence. Twelve trichothecenes (Nivalenol, Neosolanol, Fusarenone X, DAS, HT-2, FB1, FB2, FB3, T-2, Zearalenone, ZEA2, ZEA3) were in a level of 292.7 μg·kg-1, Enniantine 8.6 μg·kg-1, Sterigmatocystin 6.5 μg·kg-1, Roquefortine C, 2.9 μg·kg-1. Ochratoxin 8.8 μg·kg-1 and Mycophenolic acid at 535 μg·kg-1 were the highest content. The synthetic color present in seeds analyzed inhibited a good purification in the extracted mycotoxin by optimizing the step in HPLC-MS/MS quantification system. The information generated in this
Proximate Composition, Fatty Acid Profile and Mycotoxin Contamination in Several Varieties of Mexican Maize  [PDF]
Silvia Denise Pea Betancourt, Rey Gutiérrez Tolentino, Beatriz Schettino
Food and Nutrition Sciences (FNS) , 2017, DOI: 10.4236/fns.2017.89062
Abstract: In Mexico maize (Zea mays L.) is an important cereal due to excellent taste and nutritional value. Nutritional content and fatty acid profile has been reported in white maize, however, there are several genotypes natives (red, yellow, black, blue, pinto), and many improved hybrids maize of which little is known. Fumonisins and aflatoxins are mycotoxins present in 25% of the world’s cereals, mainly in tropical and subtropical regions around the world. This study presents the analysis of proximate composition (ash, protein, lipids), as well as mycotoxins (fumonisins and aflatoxins) and five different fatty acids, two of saturated fatty acids (palmitic and stearic) and three of polyunsaturated fatty acids (oleic, linoleic and linolenic), evaluated by Gas Chromatography and Flame Detector (GC-DF). Thirty varieties of maize (native and hybrid maize) were collected in states of central region. The total fumonisins were determined using the QuickTox TM extraction and quantificated by QuickScan fumonisins; the aflatoxins were analyzed by commercial ELISA kit. The highest protein level was 10.43 g/100g, 5.63 g/100g for fat, 1.62 g/100g for ash in hybrid maize. In native maize, the highest levels of protein and ash were 10.94 g/100g and 1.45 g/100g for pinto maize. The higher value for fat was 5.45 g/100g in yellow maize. The palmitic and stearic acids, in native maize were higher that hybrid maize, for linoleic acid and linoleinic fatty with a significant difference between native corn. Fumonisins and aflatoxins were contaminated in all genotypes of maize, in allow levels. This information obtained may be considered in maize breeding programs, industrialization processes and healthy diets.
Contenido de aflatoxinas y proteína en 13 variedades de frijol (Phaseolus vulgaris L.)
Pea-Betancourt, Silvia Denise;Conde-Martínez, Víctor;
Revista mexicana de ciencias agrícolas , 2012,
Abstract: in mexico, the bean (phaseolus vulgaris l.) is a highly consumed legume seed (11 kg per capita annually), so that its cultivation it' s quite extensive in different regions. in this study we investigated the presence of aflatoxins in eight common bean varieties and five improved bean varieties, in addition to protein and moisture. in all the varieties tested, the moisture content showed large variations (from 6 to 16%), being 16%of the varieties studied outside the normal (< 12%). the highest content of protein was detected in the improved bean varieties (26.1%) and, the lowest in commercial (19.8% ± 3.09) and wild varieties (20.78% ± 1.93). all of them showed aflatoxins on average of 7.46 ng g-1 and a range from 5 to 13 ng g-1. the highest level of contamination was observed in the improved bean varieties (9.2 ±2.9 ngg-1), followed by the commercial ones ±0.95 7.25 ngg-1 and wild varieties 6 ± 1 ng g-1. tannins were detected in wild bean varieties at a level of 0.44%± 0.13. the results obtained confirm the presence of toxic and anti-nutritional compounds in the different varieties of common and wild beans at levels permitted by national law, but may pose a risk to the consumer's health due to its high consumption.
Contenido de aflatoxinas y proteína en 13 variedades de frijol (Phaseolus vulgaris L.) Aflatoxin and protein content in 13 bean (Phaseolus vulgaris L.) varieties
Silvia Denise Pea-Betancourt,Víctor Conde-Martínez
Revista mexicana de ciencias agrícolas , 2012,
Abstract: En México el frijol (Phaseolus vulgaris L.) es una semilla leguminosa de elevado consumo (11 kg per cápita anualmente), por lo que su cultivo es amplio en diferentes regiones. En este estudió se determinó la presencia de aflatoxinas en ocho variedades de frijol común y cinco variedades de frijol mejorado; además del contenido de proteína y humedad. En todas las variedades evaluadas el contenido de humedad mostró grandes variaciones (6 a 16%), encontrándose 16% de las variedades estudiadas fuera de la normatividad (<12%). El mayor contenido de proteína se detectó en las variedades de frijol mejoradas (26.1%) y el más bajo en las variedades comerciales (19.8%, ±3.09) y silvestres (20.78%±1.93). Todas las variedades presentaron aflatoxinas en un promedio de 7.46 ng g-1 y un intervalo de 5 a 13 ng g-1. El nivel más alto de contaminación se observó en las variedades de frijol mejoradas (9.2 ±2.9 ng g-1), seguidas de las variedades comerciales 7.25 ±0.95 ng g-1 y las variedades silvestres 6 ±1 ng g-1. Se detectaron taninos en las variedades de frijol silvestre en un nivel de 0.44% ±0.13. Los resultados obtenidos confirman la presencia de compuestos tóxicos y antinutricionales en las diferentes variedades de frijol común y silvestre, en niveles permitidos por la legislación nacional; sin embargo, pueden constituir un riesgo a la salud del consumidor, debido a su alto consumo. In Mexico, the bean (Phaseolus vulgaris L.) is a highly consumed legume seed (11 kg per capita annually), so that its cultivation it' s quite extensive in different regions. In this study we investigated the presence of aflatoxins in eight common bean varieties and five improved bean varieties, in addition to protein and moisture. In all the varieties tested, the moisture content showed large variations (from 6 to 16%), being 16%of the varieties studied outside the normal (< 12%). The highest content of protein was detected in the improved bean varieties (26.1%) and, the lowest in commercial (19.8% ± 3.09) and wild varieties (20.78% ± 1.93). All of them showed aflatoxins on average of 7.46 ng g-1 and a range from 5 to 13 ng g-1. The highest level of contamination was observed in the improved bean varieties (9.2 ±2.9 ngg-1), followed by the commercial ones ±0.95 7.25 ngg-1 and wild varieties 6 ± 1 ng g-1. Tannins were detected in wild bean varieties at a level of 0.44%± 0.13. The results obtained confirm the presence of toxic and anti-nutritional compounds in the different varieties of common and wild beans at levels permitted by national law, but may pose a risk to the consumer's health d
Estrés oxidativo en gametos - Oxidative stress in gametes
Córdova-Izquierdo Alejandro,Saltijeral Oaxaca Jorge A,Ruiz Lang Gustavo,Pea Betancourt Silvia Denise
REDVET , 2010,
Abstract: ResumenEn la actualidad, la Comunidad Científica Internacional, está preocupada poraumentar la eficiencia reproductiva y productiva de los animales deimportancia económica para el hombre; preocupación dirigida a laobtención de más y mejores ganancias para el ganadero, mediante elaumento de la producción de alimentos de origen animal de mejor calidadpara el consumidor de cualquier parte del mundo. Sin embargo, lanecesidad de producir más alimentos y de mejor calidad en un tiemporelativamente corto, ha llevado a los especialistas en producción animal a laintensificación, tecnificación e industrialización de las unidades deproducción animal (UPAS) en todo el mundo, lo cual ha conducido a lapresencia de grandes aglomeraciones de los animales en las UPASinmensamente grandes; cuyas consecuencias son el estrés oxidativo crónicoy permanente para los animales, repercutiendo severamente en sudesempe o reproductivo y como consecuencia en la cantidad y calidad en la producción de gametos. Por lo cual, la Comunidad Científica Internacional,está preocupada por realizar investigaciones que conduzcan a proponeralternativas para disminuir el estrés oxidativo de los animales en las UPAS,mejorando su fertilidad, mediante la mejor producción en cantidad y calidadde gametos, factibles de ser utilizados para aprovechar mejor el potencialbiológico de los animales. En este trabajo, se presentan aspectos generalesde la importancia del estrés oxidativo en gametos.SummaryAt present, the international scientific community is concerned aboutincreasing the reproductive efficiency and productivity of animals ofeconomic importance to humans, concern directed to obtaining more andbetter profits for the farmer, through increased food production animal bestquality for the consumer from anywhere in the world. However, the need toproduce more food and better quality in a relatively short time, has ledspecialists in animal production intensification and industrializationtechnification animal production units (UPAS) throughout the world, whichhas led to the presence of large groups of animals in UPAS immenselylarge, whose consequences are chronic and permanent oxidative stress toanimals, severely impacting the reproductive performance and consequentlyon the quantity and quality in the production of gametes. Therefore, theinternational scientific community is concerned by research leading topropose alternatives to reduce oxidative stress in UPAS animals, improvingtheir fertility by better production quality and quantity of gametes that canbe used to exploit the biological potential of the ani
Illegal Use of Clenbuterol in Cattle Production in México  [PDF]
Benjamin Valladares-Carranza, Rómulo Ba?uelos-Valenzuela, Silvia D. Pea-Betancourt, Valente Velázquez-Ordo?ez, Yadira Velázquez-Armenta, Alejandro Nava-Ocampo
Health (Health) , 2014, DOI: 10.4236/health.2014.68087
Abstract: Slaughtered cattle (n = 582) from México State were bled for a serological screening of clenbuterol residues, using a commercial enzyme-linked immunosorbent assay (ELISA). Clenbuterol residues were found in a total of 153/582 (26.2%) sera analysed. These results reinforced the assumption of the illegal use of clenbuterol in cattle production in México; therefore, routine screening examinations in slaughtered cattle were strongly advised considering the toxic potential for humans.
Detección de glucomacropéptido (GMP) como indicador de adulteración con suero de quesería en leche deshidratada
Claudia Dolores Alcázar Montá?ez,Jorge Rosas Ramírez,Carlos J. Jaramillo Arango,Silvia D. Pea Betancourt
Veterinaria México , 2000,
Abstract:
Violencia contra la mujer durante el embarazo: Resumen de casos
Benítez Guerra,Gidder; Pea,Alejandra; Pea,Denise;
Revista de Obstetricia y Ginecología de Venezuela , 2004,
Abstract: gender violence is present in all the cultures and social scales and occur more frequently at home. it is a public health problem, due to the negative impact over women′s health, morbidity and mortality. we present three cases of gender violence during pregnancy, attended in the hospital universitario de caracas since 1997 at 2002.
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