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Search Results: 1 - 10 of 571 matches for " Siew Mee Hii "
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A High Molecular-Mass Anoxybacillus sp. SK3-4 Amylopullulanase: Characterization and Its Relationship in Carbohydrate Utilization
Ummirul Mukminin Kahar,Kok-Gan Chan,Madihah Md. Salleh,Siew Mee Hii,Kian Mau Goh
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140611302
Abstract: An amylopullulanase of the thermophilic Anoxybacillus sp. SK3-4 (ApuASK) was purified to homogeneity and characterized. Though amylopullulanases larger than 200 kDa are rare, the molecular mass of purified ApuASK appears to be approximately 225 kDa, on both SDS-PAGE analyses and native-PAGE analyses. ApuASK was stable between pH 6.0 and pH 8.0 and exhibited optimal activity at pH 7.5. The optimal temperature for ApuASK enzyme activity was 60 °C, and it retained 54% of its total activity for 240 min at 65 °C. ApuASK reacts with pullulan, starch, glycogen, and dextrin, yielding glucose, maltose, and maltotriose. Interestingly, most of the previously described amylopullulanases are unable to produce glucose and maltose from these substrates. Thus, ApuASK is a novel, high molecular-mass amylopullulanase able to produce glucose, maltose, and maltotriose from pullulan and starch. Based on whole genome sequencing data, ApuASK appeared to be the largest protein present in Anoxybacillus sp. SK3-4. The α-amylase catalytic domain present in all of the amylase superfamily members is present in ApuASK, located between the cyclodextrin (CD)-pullulan-degrading N-terminus and the α-amylase catalytic C-terminus (amyC) domains. In addition, the existence of a S-layer homology (SLH) domain indicates that ApuASK might function as a cell-anchoring enzyme and be important for carbohydrate utilization in a streaming hot spring.
Equilibrium and Kinetic Studies for Basic Yellow 11 Removal by Sargassum binderi
Pei-Ling Tan,Ching-Lee Wong,Siew-Teng Ong,Siew-Ling Hii
Journal of Applied Sciences , 2009,
Abstract: Dyes have been widely used in many industries such as textile, paint and also ink industry. Discharge of dyes from these industries need to undergo treatment prior of disposal to avoid ecosystem to be devastated. Upon this, Sargassum binderi, a species of brown seaweed was used as a low cost biosorbent to remove a basic dye, Basic Yellow 11 (BY11). Various parameters such as pH, initial concentration and sorbent dosage were conducted using batch sorption process. In this study, S. binderi exhibited good performance (almost 100% of BY11 removal) with 1 g of sorbent dosage in 100 mg L-1 of dye solution. pH of the solution, however, does not seem to have high influence on removal of BY11. Langmuir, Freundlich and Temkin isotherm models were used to study sorption behaviors of S. binderi at equilibrium stage. From the analysis, the sorption of BY11 onto the S. binderi was found obeyed Freundlich model with coefficient correlation (R2) value of 0.9959 which indicates heterogeneous layers of sorption. The Freundlich constant, KF and n values obtained were 32.46 mg g-1 (L mg-1)1/n and 1.968, respectively. Value of Freundlich component, n, was more than 1 indicates favourable adsorption process of this system. The kinetic sorption of BY11 using S. binderi were analyzed by using pseudo-first-order, pseudo-second-order and intraparticular diffusion model. The sorption process followed pseudo-second-order kinetic which involved chemisorption. Calculated qe values from pseudo-second-order kinetic model were found fitted well with the experimental qe values. Each R2 values from linear regression line of pseudo-second-order kinetic model were more than 0.99. In this study, S. binderi was proven to have high sorption efficiency towards BY11 and thus, it can be categorized as one of the promising biosorbent to be applied in treating the effluent discharge from industries.
Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications
Siew Ling Hii,Joo Shun Tan,Tau Chuan Ling,Arbakariya Bin Ariff
Enzyme Research , 2012, DOI: 10.1155/2012/921362
Abstract: The use of pullulanase (EC 3.2.1.41) has recently been the subject of increased applications in starch-based industries especially those aimed for glucose production. Pullulanase, an important debranching enzyme, has been widely utilised to hydrolyse the α-1,6 glucosidic linkages in starch, amylopectin, pullulan, and related oligosaccharides, which enables a complete and efficient conversion of the branched polysaccharides into small fermentable sugars during saccharification process. The industrial manufacturing of glucose involves two successive enzymatic steps: liquefaction, carried out after gelatinisation by the action of α-amylase; saccharification, which results in further transformation of maltodextrins into glucose. During saccharification process, pullulanase has been used to increase the final glucose concentration with reduced amount of glucoamylase. Therefore, the reversion reaction that involves resynthesis of saccharides from glucose molecules is prevented. To date, five groups of pullulanase enzymes have been reported, that is, (i) pullulanase type I, (ii) amylopullulanase, (iii) neopullulanase, (iv) isopullulanase, and (v) pullulan hydrolase type III. The current paper extensively reviews each category of pullulanase, properties of pullulanase, merits of applying pullulanase during starch bioprocessing, current genetic engineering works related to pullulanase genes, and possible industrial applications of pullulanase. 1. Introduction Starch is a major industrial raw material and is chemically and/or enzymatically processed into variety of products for subsequent use in various industries, ranging from food (especially high-fructose and glucose syrups) to washing detergent industries [1–3]. Starch is, after cellulose, one of the most abundant heterogeneous polysaccharide produced by plants in the form of water insoluble granules. It is a polymeric carbohydrate, composed of C, H, and O atoms in the ratio of 6?:?10?:?5, (C6H10O5)??. Molecules of starch are made of hundreds or thousands of glucose, corresponding to values of ?? that range from 50 to several thousands. Glucose units are linked to one another through C1 oxygen as glucosidic bond. Glucosidic bonds are stable under alkaline conditions while treatment of starch with acids or certain enzymes breaks the polymer into its constituent glucose molecules. The end unit of the polymeric chain has a latent aldehyde group and is known as the reducing end group. Most starches are mixture of two polymers with high molecular weight: (i) a linear chain molecule—amylose, and (ii) a branch
Production of Cyclodextrin Glycosyltransferase (CGTase) by Bacillus lehensis S8 using Sago Starch as Carbon Source
Pui-Woon Yap,Arbakariya B. Ariff,Kwan-Kit Woo,Siew-Ling Hii
Journal of Biological Sciences , 2010,
Abstract: Production of cyclodextrin glycosyltransferase (CGTase) is influenced by the reaction of the CGTase-producing strain towards various types of substrates. Variations in environmental factors such as concentrations of carbon and nitrogen sources possess significant effects on CGTase production. The present study was conducted with the prime purpose to optimise the cultivation medium in enhancing the CGTase production by a locally isolated alkalophilic Bacillus sp. The CGTase fermentation processes were performed in 250 mL Erlenmeyer flasks containing 200 mL of production medium with continuous shaking at 200 rpm and 37°C. Optimisation process was conducted by using change-a-factor-at-a-time method. From the study, an indigenous Malaysian carbon source, i.e., sago starch was found capable in improving the CGTase production with the CGTase yield of 18452 U g-1 at 0.1% w/v of starch. In addition to that, by using yeast extract as the sole nitrogen source in the medium, the CGTase excretion by the isolate is greatly enhanced as compared to the basal medium which employed two types of nitrogenous compounds. The optimised growth medium that has been successfully developed for high level of CGTase production by using the locally isolated Bacillus lehensis in 250 mL Erlenmeyer flask is comprised of (% w/v): 0.1% sago starch, 1% yeast extract, 1% sodium carbonate, 0.009% magnesium sulphate and 0.1% di-potassium hydrogen phosphate.
Is Distance Education the Answer to the Nursing Shortage?  [PDF]
Susan Mee
Open Journal of Nursing (OJN) , 2014, DOI: 10.4236/ojn.2014.43020
Abstract:

This study examined the effectiveness of distance education compared with campus based learning among nursing students. Distance education in nursing curricula is increasing. Academic nurse leaders must demonstrate the effectiveness of distance learning. This study is unique in that two cohorts, distance learners and campus learners, were taught synchronously by the same faculty member. Quantitative measures of student learning outcomes were compared using SPSS. There were no significant differences in learning outcomes between distance learners and campus learners. This study provides empiric support for distance education as a means to address the nursing shortage.

Individual Differences in Recognizing Spontaneous Emotional Expressions: Their Implications for Positive Interpersonal Relationships  [PDF]
Sun-Mee Kang
Psychology (PSYCH) , 2012, DOI: 10.4236/psych.2012.312A175
Abstract:

The main purpose of the current study was to introduce the Spontaneous Expressions Recognition Test (SERT), a new thin-slice measure of emotion recognition for normative adults, and demonstrate its relative strengths for predicting positive interpersonal relationships. To explore this question, a semester-long longitudinal study was conducted. In this study, college students were randomly assigned to small research teams and worked together throughout the semester to conduct group research projects. Peer ratings of interpersonal relationships were collected at the end of the semester. The results provided preliminary support for the SERT, by demonstrating its relative strength for predicting positive interpersonal relationships.

A RE-SURVEY OF AEDES AEGYPTI AND AEDES ALBOPICTUS IN SABAH, MALAYSIA
Feffrey Hii Lu King
Bulletin of Health Research , 2012,
Abstract: Di Sabah, Malaysia telah diadakan survey nyamuk Aedes aegypti dan Aedes albopictus dari bulan Agustus s/d September 1974 dan Maret-April 1975. Survey dilakukan pada 5 daerah di Kudat. Pantai Barat, Pedalaman Sandahan danTawan dengan tujuan untuk melihat distribusi dan density dari kedua jenis nyamuk tsb. Hasil survey menunjukkan bahwa nyamuk A. aegypti telah menyebar pada hampir seluruh daerah pantai Sabah, dan ditemukan hampir pada sebagian besar kotal dan daerah pedesaan di banyak desa. Di bandingkan dengan hasil survey yang pernah dilakukan pada tahun 1970 oleh Ramalingam ternyata terdapat pertambahan 24 persen daerah A. aegypti, dari 58.7 persen daerah A. albopictus selama 4 tahun. A. aegypti sangat biasa terdapat di desa2 pantai sebelah timur, dan ditempat-tempat lainnya menunjukkan Breteau index yang tinggi. Diduga adanya perahu2 dan tempat2 penampungan air merupakan faktor yang penting dalam penyebaran nyamuk. Walaupun tidak terdapat epidemi dengue dan demam berdarah di Sabah, tetapi ada indikasi risiko trahsmissi yang besar di daerah2 dimana Breteau index lebih dari 50. Dari hasil survey ini strategi pemberantasan A. aegypti dapat dikembangkan untuk mencegah terjadinya epidemi dengue dan demam berdarah.
Veterinary dairy herd fertility service provision in seasonal and non-seasonal dairy industries - a comparison
JF Mee
Irish Veterinary Journal , 2010, DOI: 10.1186/2046-0481-63-4-230
Abstract: A herd fertility service (HFS) is considered a basic component of modern herd health service provision in dairy industries internationally [7]. A HFS may be defined as a proactive, routine service provided to manage all aspects of bovine fertility, usually by a veterinary practitioner, with emphasis on the herd as the unit of interest, rather than the fertility of the individual animal. The approach is heuristic as well as algorithmic. It is usually provided on an appointment basis, rather than as part of an emergency call, with visits scheduled at appropriate times relative to the herd breeding pattern. The HFS may be part of a larger herd health service provided by the veterinarian [15]. While planned animal health and production has been promoted in Ireland for many years [2,9,10], the level of adoption in veterinary practice is unknown. The models of veterinary HFS provision are quite diverse between dairy industries internationally, often with little communication of ideas or practices between commercial service providers. For example, pharmacological intervention services to achieve pregnancies may be a routine practice on many North American dairies [4] but not in many European dairy herds [1]. Within Europe, contrasting dairy herd management systems operate in different countries resulting in different, largely undocumented, approaches to dairy herd fertility management. This variation in HFS between countries was the motivation for the international surveys reported here. The objective was to elicit veterinary practitioners' views on HFS provision within Ireland and to compare this with two contrasting dairy industries; The Netherlands and Portugal. In Ireland, the majority of dairy cows calve seasonally, primarily in the spring, and are bred in a low-cost, pastoral management system with emphasis on grassland management, and farmer-led fertility management predominates. In The Netherlands and in Portugal, the majority of dairy cows calve all-year-round and
E-Learning and Change in Higher Education: The Policy Environment
A. Mee
Bulgarian Journal of Science and Education Policy , 2007,
Abstract: This paper critically examines the role of government policy with respect to e-learning as an agent of change in the higher education sector. The potential impact of e-learning on the structure and organisation of the higher education sector has become a central issue for managers at institutional level and government policy makers across the globe. There is clear evidence from a range of policy documents from various nations of a general acceptance that an imperative exists to engage with the ‘knowledge economy’ in order to secure or retain a competitive economic advantage in the global order. It is seen that emerging digital technologies have a central role to play in this task and many governments have been active in promoting the development of e-learning as an agent of improvement, capacity building and organisational change in the higher education sector. Evidence is available that such technologies, supported by government policy, have served to enhance the quality of the student experience within the existing organisational and pedagogical frameworks which characterise the majority of higher education institutions. There is, however, little evidence of radical restructuring or the ‘transformation’ of existing institutions into the ‘virtual universities’ envisioned by some early commentators. This paper explores the wider policy context within which governments must work if they are to reap the full benefits of emerging technologies.
Generation of Active Bovine Terminal Deoxynucleotidyl Transferase (TdT) in E.coli
Wee Liang Kuan, Joma Joy, Ng Fui Mee, Kwek Zekui Perlyn, Then Siew Wen, Thuy Nguen, Joanne James, Elaine Chai, Horst Flotow, Sharon Crasta, Kelvin Chua, Ng Sok Peng and Jeffrey Hill
Biochemistry Insights , 2012, DOI: 10.4137/BCI.S5123
Abstract: A synthetic gene encoding bovine terminal deoxynucleotidyl transferase (TdT) was generated, cloned into an expression vector and expressed in E.coli. The effects of altering culture and induction conditions on the nature of recombinant protein production were investigated. This led to the expression of active recombinant bovine TdT in E.coli. After purification and characterisation, the activity of the enzyme was assessed in a biological assay for apoptosis. The process described in this report enables the economical production of TdT for high throughput applications.
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