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Search Results: 1 - 10 of 32830 matches for " ShunXing Guo "
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Screening for differentially expressed genes in Anoectochilus roxburghii (Orchidaceae) during symbiosis with the mycorrhizal fungus Epulorhiza sp.
Biao Li,MingJuan Tang,Kun Tang,LiFang Zhao,ShunXing Guo
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4284-0
Abstract: Mycorrhizal fungi promote the growth and development of plants, including medicinal plants. The mechanisms by which this growth promotion occurs are of theoretical interest and practical importance to agriculture. Here, an endophytic fungus (AR-18) was isolated from roots of the orchid Anoectochilus roxburghii growing in the wild, and identified as Epulorhiza sp. Tissue-cultured seedlings of A. roxburghii were inoculated with AR-18 and co-cultured for 60 d. Endotrophic mycorrhiza formed and the growth of A. roxburghii was markedly promoted by the fungus. To identify genes in A. roxburghii that were differentially expressed during the symbiosis with AR-18, we used the differential display reverse transcription polymerase chain reaction (DDRT-PCR) method to compare the transcriptomes between seedlings inoculated with the fungus and control seedlings. We amplified 52 DDRT-PCR bands using 15 primer combinations of three anchor primers and five arbitrary primers, and nine bands were re-amplified by double primers. Reverse Northern blot analyses were used to further screen the bands. Five clones were up-regulated in the symbiotic interaction, including genes encoding a uracil phosphoribosyltransferase (UPRTs; EC and a hypothetical protein. One gene encoding an amino acid transmembrane transporter was down-regulated, and one gene encoding a tRNA-Lys (trnK) and a maturase K (matK) pseudogene were expressed only in the inoculated seedlings. The possible roles of the above genes, especially the UPRTs and matK genes, are discussed in relation to the fungal interaction. This study is the first of its type in A. roxburghii.
Identification of Anoectochilus based on rDNA ITS sequences alignment and SELDI-TOF-MS
Chuan Gao, Fusheng Zhang, Jun Zhang, Shunxing Guo, Hongbo Shao
International Journal of Biological Sciences , 2009,
Abstract: The internal transcribed spacer (ITS) sequences alignment and proteomic difference of Anoectochilus interspecies have been studied by means of ITS molecular identification and surface enhanced laser desorption ionization time of flight mass spectrography. Results showed that variety certification on Anoectochilus by ITS sequences can not determine species, and there is proteomic difference among Anoectochilus interspecies. Moreover, proteomic finger printings of five Anoectochilus species have been established for identifying species, and genetic relationships of five species within Anoectochilus have been deduced according to proteomic differences among five species.
Discrimination of the rare medicinal plant Dendrobium officinale based on naringenin, bibenzyl, and polysaccharides
XiaoMei Chen,FangFei Wang,YunQiang Wang,XueLan Li,AiRong Wang,ChunLan Wang,ShunXing Guo
Science China Life Sciences , 2012, DOI: 10.1007/s11427-012-4419-3
Abstract: The aim of this study was to establish a method for discriminating Dendrobium officinale from four of its close relatives Dendrobium chrysanthum, Dendrobium crystallinum, Dendrobium aphyllum and Dendrobium devonianum based on chemical composition analysis. We analyzed 62 samples of 24 Dendrobium species. High performance liquid chromatography analysis confirmed that the four low molecular weight compounds 4′,5,7-trihydroxyflavanone (naringenin), 3,4-dihydroxy-4′,5-dime-thoxybibenzyl (DDB-2), 3′,4-dihydroxy-3,5′-dimethoxybibenzyl (gigantol), and 4,4′-dihydroxy-3,3′,5-trimethoxybibenzy (moscatilin), were common in the genus. The phenol-sulfuric acid method was used to quantify polysaccharides, and the monosaccharide composition of the polysaccharides was determined by gas chromatography. Stepwise discriminant analysis was used to differentiate among the five closely related species based on the chemical composition analysis. This proved to be a simple and accurate approach for discriminating among these species. The results also showed that the polysaccharide content, the amounts of the four low molecular weight compounds, and the mannose to glucose ratio, were important factors for species discriminant. Therefore, we propose that a chemical analysis based on quantification of naringenin, bibenzyl, and polysaccharides is effective for identifying D. officinale.
Effects of the Fungal Elicitor on the Polysaccharide Content of Dendrobium candidum Protocorms

Yang Hui,Chen Xiaomei,Guo Shunxing,
杨 慧

世界科学技术-中医药现代化 , 2009,
Abstract: The effects of MF24 fungal elicitor on the polysaccharide content of Dendrobium candidum protocorms were studied in this work. MF24 fungal elicitor was prepared in 4 ways and added at 3 dosages. Distilled water was used as the control. The elicitor was added at different time points and then co-cultured with protocorms for different time. The polysaccharide content of protocorms rose to 5.63%, as the mycelium of MF24 was used as the elicitor (elicitor T4) and added at 250 mL·L-1 culture medium (high dosage), increasing by 78.2% compared with the control (3.16%). When elicitor T4 was added at a high dosage at the 14th week and then cultured for 2 more weeks, the polysaccharide content reached the highest value of 5.92%, increasing by 29.0% compared with the average value of 4.59%. The results showed that the polysaccharide content of protocorms may be improved when the mycelium of MF24 is used as the elicitor and added at the later stage of the culture.
Genetic Diversity and Evolution of Chinese Traditional Medicinal Fungus Polyporus umbellatus (Polyporales, Basidiomycota)
Xiaoke Xing, Xueting Ma, Miranda M. Hart, Airong Wang, Shunxing Guo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0058807
Abstract: Background Polyporus umbellatus is an important medicinal fungus distributed throughout most area of China. Its wide distribution may have resulted in substantial intraspecific genetic diversity for the fungus, potentially creating variation in its medical value. To date, we know little about the intraspecific genetic diversity of P. umbellatus. Methodology/Principal Findings The objective of this research was to assess genetic differences of P. umbellatus from geographically diverse regions of China based on nrDNA ITS and 28S rRNA (LSU, large subunit) sequences. Significant sequence variations in the ITS and LSU sequences were detected. All sclerotial samples were clustered into four clades based on phylogenetic analysis of ITS, LSU and a combined data set of both regions. Heterogeneity of ITS and LSU sequences was detected in 5 and 7 samples respectively. All clone sequences clustered into the same clade except for one LSU clone sequences (from Henan province) which clustered into two clades (Clade I and Clade II). Significant genetic divergence in P. umbellatus was observed and the genetic diversification was greater among sclerotial samples from Shaanxi, Henan and Gansu provinces than among other provinces. Polymorphism of ITS and LSU sequences indicated that in China, P. umbellatus may spread from a center (Shaanxi, Henan and Gansu province) to other regions. Conclusions/Significance We found sclerotial samples of P. umbellatus contained levels of intraspecific genetic diversity. These findings suggested that P. umbellatus populations in Shaanxi, Henan and Gansu are important resources of genetic diversity and should be conserved accordingly.
Microstructure and Endophytic Fungus Distribution of Maytenus confertiflorus Root

Xiaoming Tan,Shunxing Guo,Yaqin Zhou,Jianhua Miu,

植物学报 , 2006,
Abstract: The microstructure and distribution of endophytic fungi in Maytenus confertiflorus root were studied by permanent paraffin sectioning and microscope photography. The secondary structure of the root consisted of periderm and vascular cylinders. Periderm was composed of cork, cork cambium, and phelloderm. The cork consisted of five or six layers of long cells. The secondary phloem occupied 46% of the diameter of the root and the parenchymas cells had abundant inclusions. The secondary xylem consisted of vessels, and xylary radial and some xylary parenchyma cells. Some segments of hypha, swelled hypha, pelotons and conidiophore were in colonies in cork and secondary phloem. Endophytic fungi existed in the local cells of cork and secondary phloem in the root.
A new ctenochasmatid pterosaur from the Lower Cretaceous, western Liaoning, China
Jiang, Shunxing;Wang, Xiaolin;
Anais da Academia Brasileira de Ciências , 2011, DOI: 10.1590/S0001-37652011000400011
Abstract: a nearly complete skull of a new ctenochasmatid pterosaur, pterofiltus qiui gen. et sp. nov., from the lower cretaceous deposits of liaoning, china, is described here. the specimen (ivpp v12339), was collected from the shale of the lower yixian formation (125 ma) at the zhangjiagou locality. it has the following combination of characters: about 112 teeth in total (including the upper and lower jaws); the dentition occupies more than 50% of the skull length; the anterior teeth vary in size; the mandibular symphysis is longer than half of the whole mandible length; in ventral view, an apparent symphyseal trough in the median part of the symphysis.

ZHANG Jihui,GUO Shunxing,CAO Wenqin,YANG Junshan,XIAO Peigen,

微生物学通报 , 1999,
Abstract: 从药用植物内生真菌中筛选到对植物生长有显著促进作用的粘帚霉属的一种真菌(Gliocladiumsp.简称Y菌),以它为出发菌株,进行原生质体制备与再生条件的研究。将培养48h的Y菌菌丝体经过巯基乙醇处理30min,并用1%的纤维素酶和溶壁酶混合液于28℃酶解3h,原生质体得率可达2.14X107个/mL,在含0.5M的甘露醇为稳渗剂的再生培养基上,其原生质体的再生率可达3.86x104。

Cao Wenqin,Guo Shunxing,Xu Jingtang,Zhang Jihui,Cheng Xiaomei,

菌物学报 , 1998,
Abstract: This paper studied the output and regeneration and fusion of theprotoplastS of strains mH1 and mK1 of Ganoderma lucidum. The results showed thatthe output of the protoplastS was different under different age of hyphae, enzymeconsistency and stabliser. For strain mH1, its optimal age of hyphe, enzymeconsistency and stabliser was 48hr, 3% and 0.6mol / L sucrose respechvely, the strainmK1 was 40hr, 1% and 0.4mol / L mannitol. The frequency of regenerahon of thetwo strains were the best and 3.20 X 10-2 (strains mH1) and 5.40 X 10-2 (strain mKI)sep~ly when the stabliser was 0.6mol / L sucrose. Fusion experimentS betWeen theabove tWo strains were done. The frequency of fusion, after treating with 30% PEG,was 1 .40 X 10 -2.

Fan Li Guo Shunxing Cao Wenqin Xiao Peigen Xu Jingtang,

菌物学报 , 1996,
Abstract: During a study of fungi present in cortical tissues of the roots of tropical orchids in Xishuangbannan, Yunnan, some isolates were obtained from Cymbidium sinense. One of them, morphologically belonging to Mycena, Sect. Sacchariferae, is evidently distinguished from previously described species of Mycena by its sub - fusiform pleurocystidia and caulocystidia gradually tapering towards the tip and flexous to kinked. .A new taxon, M. orchidicola, is therefore described and illustrated to accommodate this isolate. Symbiotic germination tests between seeds of 12 orchid species and M. orchidicola demonstrated M. orchidicola stimulated seed germination of 5 orchid species but it was not effective in promoting seed germination or protocorm development of other orchids. This means M. orchidicola is active and has ability to stimulate seed germination.
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