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Search Results: 1 - 10 of 5355 matches for " Shuang Geng "
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Amiloride Enhances Antigen Specific CTL by Faciliting HBV DNA Vaccine Entry into Cells
Shuang Geng, Yiwei Zhong, Shuang Wang, Hu Liu, Qiang Zou, Xiaoping Xie, Chaofan Li, Qingling Yu, Zhonghuai He, Bin Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033015
Abstract: The induction of relatively weak immunity by DNA vaccines in humans can be largely attributed to the low efficiency of transduction of somatic cells. Although formulation with liposomes has been shown to enhance DNA transduction of cultured cells, little, if any, effect is observed on the transduction of somatic tissues and cells. To improve the rate of transduction, DNA vaccine delivery by gene gun and the recently developed electroporation techniques have been employed. We report here that to circumvent requirement for such equipment, amiloride, a drug that is prescribed for hypertension treatment, can accelerate plasmid entry into antigen presenting cells (APCs) both in vitro and in vivo. The combination induced APCs more dramatically in both maturation and cytokine secretion. Amiloride enhanced development of full CD8 cytolytic function including induction of high levels of antigen specific CTL and expression of IFN-γ+perforin+granzymeB+ in CD8+ T cells. Thus, amiloride is a facilitator for DNA transduction into host cells which in turn enhances the efficiency of the immune responses.
Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells
Shuang Geng, Yang Yu, Youmin Kang, George Pavlakis, Huali Jin, Jinyao Li, Yanxin Hu, Weibin Hu, Shuang Wang, Bin Wang
BMC Immunology , 2011, DOI: 10.1186/1471-2172-12-27
Abstract: In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25- iTreg induction. Firstly, we showed that the induction of CD25- iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25- iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25- iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes.Our data thus indicate that efficient induction of CD25- iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25- iTreg for clinical applications such as flea allergic dermatitis.The inducible regulatory T cells, or iTreg, differ from the naturally regulatory T cells (nTreg) in that the former are generated in the periphery through encounter with environmental antigens. It is also believed that iTreg play non-overlapping roles, relative to nTreg, in regulating peripheral tolerance [1-3]. Most iTreg reported to date have been CD25+ cells (CD4+CD25+Foxp3+), and it is well established that their induction requires suboptimal stimulation of the T cell receptor (TCR) and cytokines TGF-β and IL-2 [3]. The CD25+ iTreg thus appear to derive primarily from weakly stimulated CD4+ T cells.We previously identified a different subset of iTreg in mice that is CD25- (CD4+CD25-Foxp3+ and IL-10+TGF-beta+IFN-γ-). The CD25- iTreg were induced after co-immunization using a protein antigen and a DNA vaccine encoding the same antigen [4-7]. Unlike that of the CD25+ iTreg, the induction of the CD25- iTreg involved the generation of CD40low IL-10high tolerogenic dendritic cells (DCs), which in turn stimulated CD25- iTreg in an antigen-specific manner [4]. We further showed in mouse models that this
Diversity of Microorganisms Isolated from the Soil Sample surround Chroogomphus rutilus in the Beijing Region
Peng Wang, Yu Liu, Yonggang Yin, Haojie Jin, Shouxian Wang, Feng Xu, Shuang Zhao, Xiaoli Geng
International Journal of Biological Sciences , 2011,
Abstract: Artificially cultivating Chroogomphus rutilus is too inefficient to be commercially feasible. Furthermore, isolating C. rutilus mycelia in the wild is difficult. Thus, it is important to determine the natural habitat of its fruiting body. This study focused on the ecology of the C. rutilus habitat to isolate and classify beneficial microorganisms that could affect its growth, which could be used in future research on artificial cultivation. In total, 342 isolates were isolated from soil samples collected around a C. rutilus colony in the Beijing region. Of these, 22 bacterial and 14 fungal isolates were selected for sequencing and phylogenetic analysis, based on their growth characteristics and colony morphology. Using 16S rRNA gene sequence analysis, the bacterial isolates were divided into two monophyletic clusters which had significant hits to the genera Bacillus and Pseudomonas, respectively. Using internal transcribed spacer (ITS) sequence analysis, fungal isolates were divided into four monophyletic clusters: Penicillium, Trichoderma, Mortierella, and Bionectria. Moreover, the phylogenetic diversity of these isolates was analysed. The results indicated that numerous microorganisms were present in C. rutilus habitat. This was the first reported examination of the microbiological ecology of C. rutilus.
Protective Effects of Extracts from Fructus rhodomyrti against Oxidative DNA Damage In Vitro and In Vivo
Yuebin Ke,Xinyun Xu,Shuang Wu,Juan Huang,Yijie Geng,Hara Misra,Yunbo Li
Oxidative Medicine and Cellular Longevity , 2013, DOI: 10.1155/2013/507407
Abstract: Objective. To evaluate the potential protective effects of extracts from Fructus rhodomyrti (FR) against oxidative DNA damage using a cellular system and the antioxidant ability on potassium bromate- (KBrO3-) mediated oxidative stress in rats. Methods. The effects of FR on DNA damage induced by hydrogen peroxide (H2O2) were evaluated by comet assay in primary spleen lymphocytes cultures. The effects of FR on the activities of SOD, CAT, and GPx and the levels of GSH, hydroperoxides, and 8-OHdG were determined in the plasma and tissues of rats treated with KBrO3. Results. FR was shown to effectively protect against DNA damage induced by H2O2??in vitro, and the maximum protective effect was observed when FR was diluted 20 times. Endogenous antioxidant status, namely, the activities of SOD, CAT, and GPx and the levels of GSH were significantly decreased in the plasma, the liver, and the kidney of the KBrO3-treated rats, while the pretreatment of FR prevented the decreases of these parameters. In addition, the pretreatment of FR was also able to prevent KBrO3-induced increases in the levels of hydroperoxides and 8-OHdG in the plasma, the liver, and the kidney in rats. Conclusions. Our findings suggested that FR might act as a chemopreventive agent with antioxidant properties offering effective protection against oxidative DNA damage in a concentration-dependent manner in vitro and in vivo. 1. Introduction Plants have played significant roles in maintaining human health and improving the quality of human life for thousands of years [1, 2]. Fructus rhodomyrti (FR) is the fruit of the Rhodomyrtus tomentosa growing on knolls in wilderness and widely distributed in Guangdong, Guangxi, Yunnan, Fujian, and Taiwan. FR has been used for the production of drinks and wine. FR is a traditional Chinese medicine material with antihepatitis property [3]. Cells that live in an oxygen-rich environment are inundated with various endogenous and exogenous sources of reactive oxygen species (ROS) [4]. The most important target for ROS in the carcinogenesis process is DNA [5, 6]. Irreparable DNA damage is involved in carcinogenesis, aging, and other degenerative diseases [4, 7]. However, enzymatic and nonenzymatic systems, which preserve the oxidant/antioxidant status, are disrupted during oxidative stress, a metabolic derangement due to an imbalance caused by excessive generation of ROS or a diminished capacity of the antioxidant defense system. Dietary factors and natural antioxidants that reduce the impact of ROS can protect DNA damage and thus reduce the risk of cancers [8,
Effects of time-varying $β$ in SNLS3 on constraining interacting dark energy models
Shuang Wang,Yong-Zhen Wang,Jia-Jia Geng,Xin Zhang
Physics , 2014, DOI: 10.1140/epjc/s10052-014-3148-0
Abstract: It has been found that, for the Supernova Legacy Survey three-year (SNLS3) data, there is strong evidence for the redshift-evolution of color-luminosity parameter $\beta$. In this paper, adopting the $w$-cold-dark-matter ($w$CDM) model and considering its interacting extensions (with three kinds of interaction between dark sectors), we explore the evolution of $\beta$ and its effects on parameter estimation. In addition to the SNLS3 data, we also take into account the Planck distance priors data of the cosmic microwave background (CMB), the galaxy clustering (GC) data extracted from SDSS DR7 and BOSS, as well as the direct measurement of Hubble constant from the Hubble Space Telescope (HST) observation. We find that, for all the interacting dark energy (IDE) models, adding a parameter of $\beta$ can reduce $\chi^2$ by $\sim$ 34, indicating that $\beta_1 = 0$ is ruled out at 5.8$\sigma$ confidence level (CL). Furthermore, it is found that varying $\beta$ can significantly change the fitting results of various cosmological parameters: for all the dark energy models considered in this paper, varying $\beta$ yields a larger $\Omega_{c0}$ and a larger $w$; on the other side, varying $\beta$ yields a smaller $h$ for the $w$CDM model, but has no impact on $h$ for the three IDE models. This implies that there is a degeneracy between $h$ and $\gamma$. Our work shows that the evolution of $\beta$ is insensitive to the interaction between dark sectors, and then highlights the importance of considering $\beta$'s evolution in the cosmology fits.
Revisit of constraints on holographic dark energy: SNLS3 dataset with the effects of time-varying $β$ and different light-curve fitters
Shuang Wang,Jia-Jia Geng,Yi-Liang Hu,Xin Zhang
Physics , 2013, DOI: 10.1007/s11433-014-5628-5
Abstract: Previous studies have shown that for the Supernova Legacy Survey three-year (SNLS3) data there is strong evidence for the redshift-evolution of color-luminosity parameter $\beta$ of type Ia supernovae (SN Ia). In this paper, we explore the effects of varying $\beta$ on the cosmological constraints of holographic dark energy (HDE) model. In addition to the SNLS3 data, we also use Planck distance prior data of cosmic microwave background (CMB), as well as galaxy clustering (GC) data extracted from Sloan Digital Sky Survey (SDSS) data release 7 and Baryon Oscillation Spectroscopic Survey (BOSS). We find that, for the both cases of using SN data alone and using SN+CMB+GC data, involving an additional parameter of $\beta$ can reduce $\chi^2$ by $\sim$ 36; this shows that $\beta$ deviates from a constant at 6$\sigma$ confidence levels. Adopting SN+CMB+GC data, we find that compared to the constant $\beta$ case, varying $\beta$ yields a larger fractional matter density $\Omega_{m0}$ and a smaller reduced Hubble constant $h$; moreover, varying $\beta$ significantly increases the value of HDE model parameter $c$, leading to $c\approx 0.8$, consistent with the constraint results obtained before Planck. These results indicate that the evolution of $\beta$ should be taken into account seriously in the cosmological fits. In addition, we find that relative to the differences between the constant $\beta$ and varying $\beta(z)$ cases, the effects of different light-curve fitters on parameter estimation are very small.
Study of Core Competence of Logistics Cluster: The Integration and the Extension of Value Chain  [PDF]
Shuang Wang
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.51003
Abstract: This paper focuses on the study of core competence of logistics cluster with the solution in two levels: one is the integration of supply chain, and the other point is the extension of value chain; both of them are based on the measurement of agglomeration level of logistics cluster and association level of cluster external resources. Hereby the MAEI model is proposed which is used to evaluate the agglomeration and association level as well as to enhance the core competence of logistics cluster by the solution of integration and extension of value chain.
A Review of the Effectiveness and Boundary Conditions of Leader Humility  [PDF]
Shuang Xu
Journal of Service Science and Management (JSSM) , 2019, DOI: 10.4236/jssm.2019.122016
Abstract: In recent years, top-down leadership style such as humility has received widespread attention in academic and practical areas. Although theoretical and empirical studies have shown that the humble leadership has a positive impact, it lacks systematic review of its mechanism and the research about boundary conditions of its positive impact. The article begins with a brief overview of the research status of humble leaders, and then combs the study of the boundary conditions of the humble leadership when it comes to individual and group levels. Finally, the future research is proposed.
Research on How to Deal with the Dilemma of Global Cooperative Governance of Cross-Border Telecom Network Fraud in China  [PDF]
Yingchao Geng
Chinese Studies (ChnStd) , 2017, DOI: 10.4236/chnstd.2017.64023
Abstract: The rapid growth of cross-border telecom network fraud in the globalized information ocean and the economic tide has posed a threat to China and the global security and stability, and its governance is imminent. Under the analysis framework of global collaborative governance, this paper analyzes the external environmental factors and related internal governance factors, such as the background of cross-border telecom network fraud governance, which needs our attention. From the level of synergetic governance and the autonomy of the subject, the analysis of this paper draws a conclusion that cross-border telecom network fraud improvement is confronted with many dilemmas, for instance, the difficulty in reaching a consensus of values, the malconformation of legal norms, the high pressure of public opinion and the weakness of human nature. The paper puts forward the countermeasures based on Chinese perspective, from four aspects of value guidance, legal soundness, system regulation and technical complementation to work with all parties to manage the main forces, to combat and prevent cross-border telecommunications network fraud, achieving a good prospect of shared governance.
Closed orbit correction of HIMM synchrotron
Geng Wang,Jian Shi,Jian-Cheng Yang,Jia-Wen Xia,Shuang Ruan,Bo Wu,He Zhao
Physics , 2015,
Abstract: The correction of closed orbit has great influence on the operation of synchrotron. The design of correction system is one significant component of lattice design. It is suggested to set BPMs at the peaks of betatron oscillation. The correctors need to be located at the positions where \b{eta} function is large or the sources of large errors. In the simulation of the closed orbit correction of HIMM synchrotron, one important reason affecting the result of horizontal correction is the longitudinal alignment error of dipole magnet. It is advisable to decrease this kind of alignment error while the deflection angle of dipole magnet is large.
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