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Search Results: 1 - 10 of 1693 matches for " Shinya Hasegawa "
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Expression of Acetaldehyde Dehydrogenase Gene Increases Hydrogen Production and Acetate Consumption by Rhodobacter sphaeroides  [PDF]
Shinya Hasegawa, Jyumpei Kobayashi, Tomoe Komoriya, Hideki Kohno, Kazuaki Yoshimune
Energy and Power Engineering (EPE) , 2015, DOI: 10.4236/epe.2015.79037
Abstract: Rhodobacter sphaeroides RV (RV) produces high yields of hydrogen from organic acids in the presence of light. The hydrogen production from acetate is lower than that from lactate, probably because of its low ability to metabolize acetate. In this study, gene of acetaldehyde dehydrogenase (ACDH, EC 1.2.1.10) that catalyzes the reversible conversion of acetaldehyde and CoA to acetyl-CoA with the concurrent reduction of NAD to NADH, is overexpressed in the RV strain. The produced acetyl-CoA can be oxidized to carbon dioxide in the tricarboxylic acid (TCA) cycle, wherein electrons are generated and used for hydrogen production. The byproduct NADH can be used as reducing agent for acetate to produce acetaldehyde by acetate dehydrogenase. The recombinant RV strain (RVAC) expressing the ACDH gene showed ACDH activity with a specific activity of 3.2 mU/ mg, and the RV and the recombinant RV strain that harbored the intact (empty) plasmid pLP-1.2 (RVI) showed no detectable ACDH activity. The hydrogen yields of the RVAC strain from 21-mM acetate were 1.5-fold higher than that of the wild type RV strain and also that of the RVI strain. In contrast, hydrogen yield from 21-mM lactate was 30% lower than that in the control strains.
Variables associated with suicide ideation and plans in a Japanese population  [PDF]
Takuya Hasegawa, Chiyoe Murata, Tatsuya Noda, Tomoko Takabayashi, Takashi Ninomiya, Shinya Hayasaka, Toshiyuki Ojima
Health (Health) , 2011, DOI: 10.4236/health.2011.35049
Abstract: The purpose of our study was to clarify variables associated with suicide ideation and plans in a Japanese population. We conducted a random-sampling survey on mental health and suicide using a self-administered questionnaire for Hamamatsu City residents aged 15-79 yrs between May and June, 2008. This included questions about gender, age, outpatient treatment, alcohol problems, depression, living ar-rangements, marital status, annual family income, industry types as well as suicide ideation and plans. The correlation between these variables and suicide ideation or plans was then analyzed with multiple logistic regression analysis by gender. A total of 1051 responded to this questionnaire (response rate, 53.9%). Variables statistically associated with suicide ideation in males included alcohol problems, depression, lower annual family income, and ac-commodations/eating/drinking services, while in females, the variables were younger age, outpatient treatment, depression, living alone, being single, being separated, lower annual family income, accommodations/eating/drinking services and unemployment. On the other hand, variables statistically associated with suicide plans in males were younger age, alcohol problems, depression, and lower annual family income, while in females they were younger age, alcohol problems, depression, being separated, lower annual family income, manufacturing, and accommodations/eating/drinking services. Ex-cept for industry types, variables associated with suicide ideation or plans were consistent with previous studies. The reason why workers engaging in manufacturing, or accommoda-tions/eating/drinking services were more likely to have suicide ideation or plans may be attributed to the structures and/or stresses unique to those industries.
(1R*,2S*,4S*,5R*)-Cyclohexane-1,2:4,5-tetracarboxylic dianhydride
Akira Uchida,Masatoshi Hasegawa,Eiichiro Takezawa,Shinya Yamaguchi
Acta Crystallographica Section E , 2012, DOI: 10.1107/s1600536812003571
Abstract: The title compound, C10H8O6, a promising raw material to obtain colorless polyimides which are applied to microelectronic and optoelectronic devices, adopts a folded conformation in which the dihedral angle between the two anhydro rings is 55.15 (8)°. The central six-membered ring assumes a conformation intermediate between boat and twist-boat. In the crystal, molecules are linked by weak C—H...O interactions, forming a layer parallel to the bc plane.
Development of 3D CAD/FEM Analysis System for Natural Teeth and Jaw Bone Constructed from X-Ray CT Images
Aki Hasegawa,Akikazu Shinya,Yuji Nakasone,Lippo V. J. Lassila,Pekka K. Vallittu,Akiyoshi Shinya
International Journal of Biomaterials , 2010, DOI: 10.1155/2010/659802
Abstract: A three-dimensional finite element model of the lower first premolar, with the three layers of enamel, dentin, and pulp, and the mandible, with the two layers of cortical and cancellous bones, was directly constructed from noninvasively acquired CT images. This model was used to develop a system to analyze the stresses on the teeth and supporting bone structure during occlusion based on the finite element method and to examine the possibility of mechanical simulation.
Development and applicability of Hospital Survey on Patient Safety Culture (HSOPS) in Japan
Shinya Ito, Kanako Seto, Mika Kigawa, Shigeru Fujita, Toshihiko Hasegawa, Tomonori Hasegawa
BMC Health Services Research , 2011, DOI: 10.1186/1472-6963-11-28
Abstract: In order to examine possibilities to introduce the Hospital Survey on Patient Safety Culture (HSOPS) in Japan, the authors of this study translated the HSOPS into Japanese, and evaluated its factor structure, internal consistency, and construct validity. Healthcare workers (n = 6,395) from 13 acute care general hospitals in Japan participated in this survey.Confirmatory factor analysis indicated that the Japanese HSOPS' 12-factor model was selected as the most pertinent, and showed a sufficiently high standard partial regression coefficient. The internal reliability of the subscale scores was 0.46-0.88. The construct validity of each safety culture sub-dimension was confirmed by polychoric correlation, and by an ordered probit analysis.The results of the present study indicate that the factor structures of the Japanese and the American HSOPS are almost identical, and that the Japanese HSOPS has acceptable levels of internal reliability and construct validity. This shows that the HSOPS can be introduced in Japan.The past 10 years have witnessed an increasing interest in safety and quality issues in healthcare. Patient safety, including the measurement of patient safety culture, has become a top priority for health systems in developed nations [1].Safety culture is defined as "the product of individual and group values, attitudes, perceptions, competencies, and patterns of behavior that determine the commitment to, and the style and proficiency of, an organization's health and safety management" [2]. Organizations with a favorable safety culture are characterized by communications founded on mutual trust, by shared perceptions of the importance of safety, and by confidence in the efficacy of preventive measures [2]. Implementation of a patient safety culture is thought to minimize adverse events and eliminate preventable harm in health care organizations.A number of self-report questionnaires have been developed to measure the patient safety climate in hospitals [3-6]
Association between Genetic Polymorphisms in Cav2.3 (R-type) Ca2+ Channels and Fentanyl Sensitivity in Patients Undergoing Painful Cosmetic Surgery
Soichiro Ide, Daisuke Nishizawa, Ken-ichi Fukuda, Shinya Kasai, Junko Hasegawa, Masakazu Hayashida, Masabumi Minami, Kazutaka Ikeda
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070694
Abstract: Individual differences in the sensitivity to fentanyl, a widely used opioid analgesic, lead to different proper doses of fentanyl, which can hamper effective pain treatment. Voltage-activated Ca2+ channels (VACCs) play a crucial role in the nervous system by controlling membrane excitability and calcium signaling. Cav2.3 (R-type) VACCs have been especially thought to play critical roles in pain pathways and the analgesic effects of opioids. However, unknown is whether single-nucleotide polymorphisms (SNPs) of the human CACNA1E (calcium channel, voltage-dependent, R type, alpha 1E subunit) gene that encodes Cav2.3 VACCs influence the analgesic effects of opioids. Thus, the present study examined associations between fentanyl sensitivity and SNPs in the human CACNA1E gene in 355 Japanese patients who underwent painful orofacial cosmetic surgery, including bone dissection. We first conducted linkage disequilibrium (LD) analyses of 223 SNPs in a region that contains the CACNA1E gene using genomic samples from 100 patients, and a total of 13 LD blocks with 42 Tag SNPs were observed within and around the CACNA1E gene region. In the preliminary study using the same 100 genomic samples, only the rs3845446 A/G SNP was significantly associated with perioperative fentanyl use among these 42 Tag SNPs. In a confirmatory study using the other 255 genomic samples, this SNP was also significantly associated with perioperative fentanyl use. Thus, we further analyzed associations between genotypes of this SNP and all of the clinical data using a total of 355 samples. The rs3845446 A/G SNP was associated with intraoperative fentanyl use, 24 h postoperative fentanyl requirements, and perioperative fentanyl use. Subjects who carried the minor G allele required significantly less fentanyl for pain control compared with subjects who did not carry this allele. Although further validation is needed, the present findings show the possibility of the involvement of CACNA1E gene polymorphisms in fentanyl sensitivity.
Association between KCNJ6 (GIRK2) Gene Polymorphisms and Postoperative Analgesic Requirements after Major Abdominal Surgery
Daisuke Nishizawa, Makoto Nagashima, Ryoji Katoh, Yasuo Satoh, Megumi Tagami, Shinya Kasai, Yasukazu Ogai, Wenhua Han, Junko Hasegawa, Naohito Shimoyama, Ichiro Sora, Masakazu Hayashida, Kazutaka Ikeda
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0007060
Abstract: Opioids are commonly used as effective analgesics for the treatment of acute and chronic pain. However, considerable individual differences have been widely observed in sensitivity to opioid analgesics. We focused on a G-protein-activated inwardly rectifying potassium (GIRK) channel subunit, GIRK2, that is an important molecule in opioid transmission. In our initial polymorphism search, a total of nine single-nucleotide polymorphisms (SNPs) were identified in the whole exon, 5′-flanking, and exon-intron boundary regions of the KCNJ6 gene encoding GIRK2. Among them, G-1250A and A1032G were selected as representative SNPs for further association studies. In an association study of 129 subjects who underwent major open abdominal surgery, the A/A genotype in the A1032G SNP and -1250G/1032A haplotype were significantly associated with increased postoperative analgesic requirements compared with other genotypes and haplotypes. The total dose (mean±SEM) of rescue analgesics converted to equivalent oral morphine doses was 20.45±9.27 mg, 10.84±2.24 mg, and 13.07±2.39 mg for the A/A, A/G, and G/G genotypes in the A1032G SNP, respectively. Additionally, KCNJ6 gene expression levels in the 1032A/A subjects were significantly decreased compared with the 1032A/G and 1032G/G subjects in a real-time quantitative PCR analysis using human brain tissues, suggesting that the 1032A/A subjects required more analgesics because of lower KCNJ6 gene expression levels and consequently insufficient analgesic effects. The results indicate that the A1032G SNP and G-1250A/A1032G haplotype could serve as markers that predict increased analgesic requirements. Our findings will provide valuable information for achieving satisfactory pain control and open new avenues for personalized pain treatment.
Measurement of Surface SH-Wave Velocities Generated on the Surface of Japanese Cypress Column  [PDF]
Masumi Hasegawa, Junji Matsumura
Open Journal of Acoustics (OJA) , 2014, DOI: 10.4236/oja.2014.44018
Abstract: Polyetherimide resin wedge transducers were used to generate a shear wave that was obliquely incident relative to the surface of a Japanese cypress column for measuring the surface SH-wave velocity. As the inter-transducer distance increased, the propagation time increased and the am-plitude became smaller. The propagation time and the amplitude were significantly correlated with the inter-transducer distance. The SH-wave velocity ranged from 1270 m/s to 1496 m/s. Surface SH-wave velocity was lower in the central part of the column and higher in the outer part. Velocity was negatively correlated with moisture content at 1% of significance level. These results suggest the accomplishment of the first target for applying the surface SH-wave acoustoelastic technique to nondestructive evaluation of drying stress in wood.
Active Stall Control System on NACA0012 by Using Synthetic Jet Actuator  [PDF]
Hiroaki Hasegawa, Shigeru Obayashi
Journal of Flow Control, Measurement & Visualization (JFCMV) , 2019, DOI: 10.4236/jfcmv.2019.71005
Abstract: Flow separation is typically an undesirable phenomenon, and boundary layer control is an important technique for the separation problems on airfoils. The synthetic jet actuator is considered as a promising candidate for flow control applications because of its compact nature and ability to generate momentum without the need for fluidic plumbing. In the present study, an active separation control system using synthetic jets is proposed and practically applied to the stall control of the NACA0012 airfoil in a wind tunnel test. In our proposed system, the flow conditions (stalled or unstalled) can be judged by calculating from two static pressure holes on the airfoil upper surface alone. The experimental results indicate that the maximum lift coefficient increases by 11% and the stall angle rises by 4° in contrast to the case under no control. It is confirmed that our proposed system can suppress the stall on the NACA0012 airfoil and that the aerodynamic performance of the airfoil can be enhanced. The proposed system can also be operated prior to the onset of stall. Therefore, separation control is always attained with no stall for all flow fields produced by changing the angle of attack that were examined.
Atomic force microscopy and Raman scattering spectroscopy studies on heat-induced fibrous aggregates of β-lactoglobulin
Shinya Ikeda
Spectroscopy: An International Journal , 2003, DOI: 10.1155/2003/920321
Abstract: Nanometer-thick fibrous aggregates of β-lactoglobulin alone and its mixture with other globular proteins were formed by heating aqueous solutions at pH 2 with maintaining an effective level of electrostatic repulsion among denatured protein molecules. In atomic force microscopy (AFM) images, these fibrous aggregates appeared to be fairly uniform in width and height and composed of strings of globular elements. Fibrous aggregates formed in β-lactoglobulin individual systems were only slightly thicker than the size of the native β-lactoglobulin monomer, while those formed in the presence of other globular proteins were more than twice thicker, suggesting that different species of globular proteins were incorporated into each individual fibrous aggregate in the mixed systems. At neutral pH, aggregates were generally composed of ellipsoidal primary particles much larger than the size of the monomer, suggesting that aggregation proceeds in two steps at neutral pH. Molecular structural changes probed by Raman scattering spectroscopy revealed that considerable fractions of β-sheet structures remained to be folded during the formation of fibrous aggregates but α-helix structures were partially lost. It was also suggested that a limited extent of hydrophobic interactions among heat-denatured protein molecules is required for the fibrous aggregation.
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