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Search Results: 1 - 10 of 29269 matches for " Shin-Hae Lee "
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Overexpression of Fatty-Acid- -Oxidation-Related Genes Extends the Lifespan of Drosophila melanogaster
Shin-Hae Lee,Su-Kyung Lee,Donggi Paik,Kyung-Jin Min
Oxidative Medicine and Cellular Longevity , 2012, DOI: 10.1155/2012/854502
Abstract: A better understanding of the aging process is necessary to ensure that the healthcare needs of an aging population are met. With the trend toward increased human life expectancies, identification of candidate genes affecting the regulation of lifespan and its relationship to environmental factors is essential. Through misexpression screening of EP mutant lines, we previously isolated several genes extending lifespan when ubiquitously overexpressed, including the two genes encoding the fatty-acid-binding protein and dodecenoyl-CoA delta-isomerase involved in fatty-acid β-oxidation, which is the main energy resource pathway in eukaryotic cells. In this study, we analyzed flies overexpressing the two main components of fatty-acid β-oxidation, and found that overexpression of fatty-acid-β-oxidation-related genes extended the Drosophila lifespan. Furthermore, we found that the ability of dietary restriction to extend lifespan was reduced by the overexpression of fatty-acid-β-oxidation-related genes. Moreover, the overexpression of fatty-acid-β-oxidation-related genes enhanced stress tolerance to oxidative and starvation stresses and activated the dFOXO signal, indicating translocation to the nucleus and transcriptional activation of the dFOXO target genes. Overall, the results of this study suggest that overexpression of fatty-acid-β-oxidation-related genes extends lifespan in a dietary-restriction-related manner, and that the mechanism of this process may be related to FOXO activation.
Overexpression of Fatty-Acid- -Oxidation-Related Genes Extends the Lifespan of Drosophila melanogaster
Shin-Hae Lee,Su-Kyung Lee,Donggi Paik,Kyung-Jin Min
Oxidative Medicine and Cellular Longevity , 2012, DOI: 10.1155/2012/854502
Abstract: A better understanding of the aging process is necessary to ensure that the healthcare needs of an aging population are met. With the trend toward increased human life expectancies, identification of candidate genes affecting the regulation of lifespan and its relationship to environmental factors is essential. Through misexpression screening of EP mutant lines, we previously isolated several genes extending lifespan when ubiquitously overexpressed, including the two genes encoding the fatty-acid-binding protein and dodecenoyl-CoA delta-isomerase involved in fatty-acid β-oxidation, which is the main energy resource pathway in eukaryotic cells. In this study, we analyzed flies overexpressing the two main components of fatty-acid β-oxidation, and found that overexpression of fatty-acid-β-oxidation-related genes extended the Drosophila lifespan. Furthermore, we found that the ability of dietary restriction to extend lifespan was reduced by the overexpression of fatty-acid-β-oxidation-related genes. Moreover, the overexpression of fatty-acid-β-oxidation-related genes enhanced stress tolerance to oxidative and starvation stresses and activated the dFOXO signal, indicating translocation to the nucleus and transcriptional activation of the dFOXO target genes. Overall, the results of this study suggest that overexpression of fatty-acid-β-oxidation-related genes extends lifespan in a dietary-restriction-related manner, and that the mechanism of this process may be related to FOXO activation. 1. Introduction The trend towards increased life expectancy demands a greater understanding of the aging process to ensure that healthcare needs of an aging population are met. This goal requires identification of the so-called “longevity candidate genes,” which are potential genes important to the regulation of lifespan, as well as appropriate understanding of how the effects of these genes are modulated by environmental factors such as diet. Numerous longevity candidate genes have been identified in model systems using extended longevity mutant phenotypes, offering important insights into the mechanisms of aging and lifespan determination [1–5]. Insulin/insulin-like growth factor (IGF) signaling (IIS), a major nutrient-sensing pathway, is a well-characterized age-related pathway. The loss of IIS function by mutations affecting insulin/IGF receptor, phosphatidylinositol-3 kinase (PI3K), Akt, and forkhead box (FOXO) has been found to extend the lifespan of C. elegans, Drosophila, and mammals [3, 6–11]. In addition, energy-sensing pathways such as those associated with
Differences in Skin Properties of Korean Women at the Initial Aging Phase  [PDF]
Gae Won Nam, Eun Joo Kim, Yu Chul Jung, Choon Bok Jeong, Kye Ho Shin, Hae Kwang Lee
Journal of Cosmetics, Dermatological Sciences and Applications (JCDSA) , 2014, DOI: 10.4236/jcdsa.2014.41007
Abstract:


Many studies on aging have focused on evaluating differences between older and younger people, but only a few have focused on differences in skin properties among subjects from the same age group according to their skin aging status. In this study, we evaluated the facial skin condition and life style factors in 110 Korean women aged 25 to 35 in an attempt to evaluate factors which may affect the skin aging status in the initial aging phase. The facial skin condition of 110 healthy Korean women was assessed over two successive 6-month periods, summer and winter. Using clinical assessments including aging, wrinkles and skin’s elasticity values, the subjects were divided into 7 groups. Then, various facial skin conditions and life style factors were examined between a severe aging group and mild aging group. In the severe aging group, the mean value pH was lower and the mean value of water content was slightly lower than that of women in the mild aging group. Also, the seasonal site variation in water content and sebum secretion level were significantly higher in the severe aging group than in the mild aging group. Topical sunscreen using percentage was not significantly different between the two groups. However, the number of cosmetic subject use was slightly higher in the mild aging group than in the severe aging group. The study suggested that there were several differences in skin characteristics between women in the severe aging group and in the mild aging group at the initial aging phase. Seasonal site variation between cheek and forehead was the most dominant differences. We also considered that life style factors such as cosmetic use could affect skin aging status.



Identification of protein functions using a machine-learning approach based on sequence-derived properties
Bum Lee, Moon Shin, Young Oh, Hae Oh, Keun Ryu
Proteome Science , 2009, DOI: 10.1186/1477-5956-7-27
Abstract: A highly accurate prediction method capable of identifying protein function, based solely on protein sequence properties, is described. This method analyses and identifies specific features of the protein sequence that are highly correlated with certain protein functions and determines the combination of protein sequence features that best characterises protein function. Thirty-three features that represent subtle differences in local regions and full regions of the protein sequences were introduced. On the basis of 484 features extracted solely from the protein sequence, models were built to predict the functions of 11 different proteins from a broad range of cellular components, molecular functions, and biological processes. The accuracy of protein function prediction using random forests with feature selection ranged from 94.23% to 100%. The local sequence information was found to have a broad range of applicability in predicting protein function.We present an accurate prediction method using a machine-learning approach based solely on protein sequence properties. The primary contribution of this paper is to propose new PNPRD features representing global and/or local differences in sequences, based on positively and/or negatively charged residues, to assist in predicting protein function. In addition, we identified a compact and useful feature subset for predicting the function of various proteins. Our results indicate that sequence-based classifiers can provide good results among a broad range of proteins, that the proposed features are useful in predicting several functions, and that the combination of our and traditional features may support the creation of a discriminative feature set for specific protein functions.The need to analyse the massive accumulation of biological data generated by high-throughput human genome projects has stimulated the development of new and rapid computational methods. Computational approaches for predicting and classifying protei
Path Renewal Method in Filtering Based Wireless Sensor Networks
Jin Myoung Kim,Young Shin Han,Hae Young Lee,Tae Ho Cho
Sensors , 2011, DOI: 10.3390/s110201396
Abstract: In applications of wireless sensor networks, there are many security issues. Attackers can create false reports and transmit the reports to the networks. These false reports can lead not only false alarms, but also the depletion of limited energy resources. In order to filter out such false reports during the forwarding process, Ye et al. proposed the statistical en-route filtering (SEF). Several research efforts to enhance the efficiency of SEF have been made. Especially, the path selection method proposed by Sun et al. can improve the detection power of SEF by considering the information on the filtering keys of and distances of upstream paths. However, such selection mechanism could lead to favored paths in heavy traffic, which would result in unbalanced energy consumption. In this paper, we propose a path renewal method to provide load balancing for sensor networks in terms of energy consumption. In our method, a node renews its upstream path to save energy resources if the remaining energy of and the communication traffic of the node exceed some threshold values. We show the effectiveness of the proposed method in terms of balanced energy consumption and filtering power by providing simulation results.
Molecular and Cellular Changes in the Lumbar Spinal Cord following Thoracic Injury: Regulation by Treadmill Locomotor Training
Hae Young Shin, Hyosil Kim, Min Jung Kwon, Dong Hoon Hwang, KiYoung Lee, Byung Gon Kim
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088215
Abstract: Traumatic spinal cord injury (SCI) often leads to debilitating loss of locomotor function. Neuroplasticity of spinal circuitry underlies some functional recovery and therefore represents a therapeutic target to improve locomotor function following SCI. However, the cellular and molecular mechanisms mediating neuroplasticity below the lesion level are not fully understood. The present study performed a gene expression profiling in the rat lumbar spinal cord at 1 and 3 weeks after contusive SCI at T9. Another group of rats received treadmill locomotor training (TMT) until 3 weeks, and gene expression profiles were compared between animals with and without TMT. Microarray analysis showed that many inflammation-related genes were robustly upregulated in the lumbar spinal cord at both 1 and 3 weeks after thoracic injury. Notably, several components involved in an early complement activation pathway were concurrently upregulated. In line with the microarray finding, the number of microglia substantially increased not only in the white matter but also in the gray matter. C3 and complement receptor 3 were intensely expressed in the ventral horn after injury. Furthermore, synaptic puncta near ventral motor neurons were frequently colocalized with microglia after injury, implicating complement activation and microglial cells in synaptic remodeling in the lumbar locomotor circuitry after SCI. Interestingly, TMT did not influence the injury-induced upregulation of inflammation-related genes. Instead, TMT restored pre-injury expression patterns of several genes that were downregulated by injury. Notably, TMT increased the expression of genes involved in neuroplasticity (Arc, Nrcam) and angiogenesis (Adam8, Tie1), suggesting that TMT may improve locomotor function in part by promoting neurovascular remodeling in the lumbar motor circuitry.
Oncoprotein HCCR-1 expression in breast cancer is well correlated with known breast cancer prognostic factors including the HER2 overexpression, p53 mutation, and ER/PR status
Seon-Ah Ha, Youn Lee, Seung Shin, Hyun Kim, Sanghee Kim, Hong Namkoong, Hae Kim, Sang Jung, Yu Lee, Yeun Chung, Sang Jung, Jin Kim
BMC Cancer , 2009, DOI: 10.1186/1471-2407-9-51
Abstract: Oncogene HCCR-1 expression levels were determined in normal breast tissues, breast cancer tissues and cancer cell lines. We examined whether HCCR-1 protein expression in breast cancer is related to different biological characteristics, including ER, PR, p53 genotype, and HER2 status in 104 primary breast cancer tissues using immunohistochemical analyses.HCCR-1 was upregulated in breast cancer cells and tissues compared with normal breast tissues. In this study, overexpression of HCCR-1 was well correlated with known breast cancer prognostic markers including the presence of steroid receptors (ER and PR), p53 mutation and high HER2 overexpression. HCCR-1 was not detected in the ER-negative, PR-negative, p53 negative and low HER2 breast cancer tissues. These data indicate that the level of HCCR-1 in breast cancer tissues is relatively well correlated with known breast cancer factors, including the HER2 overexpression, p53 mutation, and ER/PR status.Determination of HCCR-1 levels as options for HER2 testing is promising although it needs further evaluation.Cells expressing HCCR-1 are tumorigenic in nude mice [1]. The functional role of this oncogene in tumorigenesis is manifested as a negative regulator of the p53 tumor suppressor [1]. In the previous study, we investigated HCCR-1 protein expression in breast cancer and the possibility of using HCCR-1 as a useful biomarker for human breast cancer [2]. We also examined whether HCCR-1 protein expression in breast cancer is related to different biological characteristics including ER, PR, p53 genotype, and the HER2 status. Northern and Western blot analyses and immunohistochemical studies indicate that the HCCR-1 mRNA and protein are overexpressed in breast cancer tissues compared to the normal breast tissues [1,2]. Serological studies revealed an 86.8% sensitivity for HCCR-1 in breast cancer, which was higher than 21.0% for CA15-3 [2,3]. The HCCR-1 assay has an advantage over CA15-3 in diagnosing breast cancer. These res
A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle
Hyun Cheong, Du-Hak Yoon, Byung Park, Lyoung Kim, Joon Bae, Sohg Namgoong, Hae Lee, Chang Han, Ji Kim, Il-Cheong Cheong, Hyoung Shin
BMC Genetics , 2008, DOI: 10.1186/1471-2156-9-33
Abstract: By direct DNA sequencing in 24 unrelated Korean cattle, we identified 39 sequence variants within exons and their flanking regions in CAPN1. Among them, 12 common polymorphic sites were selected for genotyping in the beef cattle (n = 421). Statistical analysis revealed that a polymorphism in the 3'UTR (c.2151*479C>T) showed significant association with MS (Pcor. = 0.02).Our findings suggest that polymorphisms in CAPN1 might be one of the important genetic factors involved in carcass quality in beef cattle, although it could be false positive association.Genetic improvement has long been considered an important factor in the competitiveness of beef cattle production. Identification of the genes and/or polymorphisms underlying quantitative/qualitative traits, and an understanding of how these genes/polymorphisms interact with the environment or with other genes affecting economic traits might be the keys to successful application of marker-assisted selection in the commercial animal population. As one of these economic traits, marbling is intramuscular fat that gives meat flavor and tenderness. Thus, an increase in the degree of marbling raises the level of meat quality.Calpain is a ubiquitous cytoplasmic cysteine protease, the activity of which is absolutely dependent on calcium [1]. Two genes of calpain (CAPN1 [macro-calpain] and CAPN2 [mili-calpain]) have been identified [2]. CAPN1 degrades myofibrillar proteins under postmortem conditions and appears to be the primary enzyme in the postmortem tenderization process [3-6]. Regulation of CAPN1 activity has been correlated with variation in meat tenderness, and previous studies also identified a quantitative trait locus influencing meat tenderness on chromosome 29 where CAPN1 lies [7-9].In the CAPN1 gene, more than 100 single nucleotide polymorphisms (SNPs) have been identified in Bos indicus or Bos taurus [8,10,11] (S. N. White and T. Smith, unpublished data). Among them, four polymorphisms, two non-synonymous SNPs (
Trend Analysis of Water Pollutant at Summer Rainfall Season  [PDF]
Youngshin Lee, Sanghee Shin
Journal of Environmental Protection (JEP) , 2014, DOI: 10.4236/jep.2014.54026
Abstract:

This study, with Hongdong Reservoir, is intended to evaluate the changes in water quality in the lake before and after rainfall in summer. Various non-point source pollutions are scattered around the reservoir, and to determine the pollution level by pollution source, samples were taken at the same point before rainfall (1st), during rainfall (2nd) and after rainfall (3rd) and concentration was measured. Pollutant concentration curve at the measuring points (HDS1, HDS2, HDS3) appeared to be similar with the hydrological curve. When comparing the concentration immediately before rainfall with event mean concentration (EMC), SS and COD were 4 - 59 times and 1 - 4 times, respectively. However, when it comes to total nitrogen (T-N), concentration arrived at the reservoir stayed the range of 1.3 - 12.0 mg/L in all 3 cases without significant variation, which indicated that total nitrogen load is critical when arriving at the reservoir, irrespective of rainfall, and thus it’s necessary to consider non-point source pollution runoff also in addition to point source pollution when developing the water quality improvement measures in reservoir.

Renegotiating the KOREA-US FTA. American Hegemony and the
Lee Hae-young
Asia-Pacific Journal : Japan Focus , 2010,
Abstract: On December 3, 2010 South Korea and the United States announced that they had reached agreement on a new KOREA-US FTA with comprise agreements on both automobiles and agriculture. The agreement may face difficult hurdles in the legislatures of both countries that must ratify it. This article examines the process and politics that led to the agreement.Whether the Free Trade Agreement reached between South Korea and the United States (KORUS FTA) prior to the November 12 Seoul G20 Summit constituted a hasty "renegotiation" of the 2007 Treaty to comply with US demands on the eve of President Barak Obama's visit to Seoul, is no longer just a matter of semantics. Of course, it is apparent why the Lee Myung-bak administration -- as well as certain news outlets under its influence -- has doggedly insisted on referring to the negotiations by a different name. First, this was to hide the fact that the administration's declaration of "no renegotiation" had failed. Second, it was a kind of ploy to avoid a National Assembly review, the argument being that no such review was required since this was not "renegotiation." However hard the Lee administration has worked to conceal it, the U.S. press with its freedom of speech has provided a detailed account of virtually all matters. And one can even find a fair amount of information trickling through the South Korean press as well.It took fierce resistance to renegotiation in South Korea to prevent the two presidents from announcing the conclusion of the FTA as planned during Obama's visit to Seoul for the G20 Summit.
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