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Search Results: 1 - 10 of 4582 matches for " Shin Mizukami "
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Photochemical Reactions of Microcystin-LR Following Irradiation with UV Light  [PDF]
Yoshihiro Mizukami
Open Journal of Physical Chemistry (OJPC) , 2016, DOI: 10.4236/ojpc.2016.64008
Abstract: Photochemical reactions of microcystin-LR, a toxic compound produced by some blue green algae, were investigated. Ultraviolet absorption of microcystin-LR was assessed. Time-dependent density functional theory (TDDFT) calculations indicated that absorption peak at 238 nm was mainly due to excitation of electrons from the linear chain structure Adda of microcystin-LR. Irradiation of microcystin-LR with UV light resulted in the reduction of the 238 nm absorption peak and the appearance of a new peak at 300 nm. Density functional theory (DFT) and TDDFT calculations with a model molecule suggested that this 300 nm peak was due to tricyclo-Adda microcystin-LR, an intermediate in photochemical reactions of microcystin-LR. Analysis of the rate of this photochemical reaction showed that it was a first order reaction.
Effects of small interfering RNA targeting thymidylate synthase on survival of ACC3 cells from salivary adenoid cystic carcinoma
Takashi Shirasaki, Shin-ichiro Maruya, Hiroki Mizukami, Seiji Kakehata, Hidekachi Kurotaki, Soroku Yagihashi, Hideichi Shinkawa
BMC Cancer , 2008, DOI: 10.1186/1471-2407-8-348
Abstract: An ACC cell line (ACC3) was transfected with siRNA targeting the TS gene and inhibition of cell growth and induction of apoptosis-associated molecules were evaluated in vitro. In addition, the in vivo effect of TS siRNA on tumor progression was assessed using a xenograft model.Our results demonstrated that ACC3 cells showed significantly higher TS expression than non-cancer cell lines and the induction of TS siRNA led to inhibition of cell proliferation. The effect was associated with an increase in p53, p21, and active caspase-3 and S-phase accumulation. We also found up-regulation of spermidine/spermine N1-acetyltransferase (SSAT), a polyamine metabolic enzyme. Furthermore, treatment with TS siRNA delivered by atelocollagen showed a significant cytostatic effect through the induction of apoptosis in a xenograft model.TS may be an important therapeutic target and siRNA targeting TS may be of potential therapeutic value in ACC.Adenoid cystic carcinoma (ACC) is one of the most common salivary gland carcinomas and has indolent characteristics and an ultimately fatal outcome. Although ACC is thought to typically grow slowly, late occurrence of locoregional recurrence and distance metastasis is very common because of a propensity for perineural invasion. ACC shows various histological patterns including tubular, cribriform, and solid types. It has been widely accepted that the prognosis for tumors that predominantly comprise solid patterns is worse than that of those with a tubular or cribriform pattern [1]. Several molecules, including p16, p27, and E-cadherin, have been proposed as prognostic markers [2-4], but markers useful as therapeutic targets have not been identified in ACC.Thymidylate synthase (TS), a folate-dependent enzyme, plays a crucial role in DNA biosynthesis. TS catalyzes the transfer of one methyl group from methylenetetrahydrofolate to generate deoxythymidine-5'-monophosphate (dTMP) from 2'-deoxyuridine-5'-monophosphate (dUMP). dTMP is then phosphoryl
Differential expression of topoisomerase IIα protein in salivary gland carcinomas: histogenetic and prognostic implications
Shin-ichiro Maruya, Takashi Shirasaki, Takahiko Nagaki, Seiji Kakehata, Hidekachi Kurotaki, Hiroki Mizukami, Hideichi Shinkawa
BMC Cancer , 2009, DOI: 10.1186/1471-2407-9-72
Abstract: The protein expression of topoIIα was evaluated immunohistochemically in formalin-fixed, paraffin-embedded tissue from 54 salivary gland carcinomas and 20 benign tumors (10 pleomorphic adenomas and 10 Warthin's tumors). The primary salivary gland carcinoma specimens consisted of 17 adenoid cystic carcinomas, 7 adenocarcinomas not otherwise specified, 7 mucoepidermoid carcinomas, 6 salivary duct carcinomas, 3 acinic cell carcinomas, 3 carcinomas ex pleomorphic adenomas, 3 epithelial-myoepithelial carcinomas, 2 carcinosarcomas, 2 lymphoepithelial carcinomas, 2 myoepithelial carcinomas, 1 oncocytic carcinoma, and 1 squamous cell carcinoma. The associations between clinicopathological factors and outcome were analyzed.Of the 54 primary salivary gland carcinomas, 38 (70%) showed positive expression (≥10%) of topoIIα protein, and 16 carcinomas (30%) and all benign tumors were negative (p < 0.001). Expression of topoIIα was more frequently observed in salivary duct carcinoma, carcinoma ex pleomorphic adenoma, adenocarcinoma, and adenoid cystic carcinoma, solid type, and it was associated with advanced stage and shortened survival.The results of the present study suggest that topoIIα expression is associated with histologically aggressive subtypes and shortened survival. Furthermore, it may provide useful prognostic information and suggests the potential efficacy of topoIIα-targeting therapy in patients with salivary gland carcinoma.Salivary gland carcinomas are uncommon neoplasms, accounting for approximately 5% of those arising in the head and neck region [1]. These tumors are characterized by widely varied histological features with heterogeneous and unpredictable clinical behaviors. In general, these carcinomas may be categorized into low grade and high grade malignancies based on their origin from either the terminal (intercalated) or the excretory ductal epithelium, respectively [2]. Biological and clinical classifications have been dependent on this histogenetic clas
Relativistic Internally Contracted Multireference Electron Correlation Methods
Toru Shiozaki,Wataru Mizukami
Physics , 2015,
Abstract: We report internally contracted relativistic multireference configuration interaction (ic-MRCI), complete active space second-order perturbation (CASPT2), and strongly contracted n-electron valence state perturbation theory (NEVPT2) on the basis of the four-component Dirac Hamiltonian, enabling accurate simulations of relativistic, quasi-degenerate electronic structure of molecules containing transition-metal and heavy elements. Our derivation and implementation of ic-MRCI and CASPT2 are based on an automatic code generator that translates second-quantized ansatze to tensor-based equations, and to efficient computer code. NEVPT2 is derived and implemented manually. The rovibrational transition energies and absorption spectra of HI and TlH are presented to demonstrate the accuracy of these methods.
Back-to-Back Comparison of Auto-Fluorescence Imaging (AFI) Versus High Resolution White Light Colonoscopy for Adenoma Detection
Kentaro Moriichi, Mikihiro Fujiya, Ryu Sato, Jiro Watari, Yoshiki Nomura, Toshie Nata, Nobuhiro Ueno, Shigeaki Maeda, Shin Kashima, Kentaro Itabashi, Chisato Ishikawa, Yuhei Inaba, Takahiro Ito, Kotaro Okamoto, Hiroki Tanabe, Yusuke Mizukami, Yusuke Saitoh, Yutaka Kohgo
BMC Gastroenterology , 2012, DOI: 10.1186/1471-230x-12-75
Abstract: This study enrolled 88 patients who underwent colonoscopy at Asahikawa Medical University and Kushiro Medical Association Hospital. A randomly selected colonoscopist first observed the sigmoid colon and rectum with conventional high resolution endosopy (HRE). Then the colonoscopist changed the mode to AFI and handed to the scope to another colonoscopist who knew no information about the HRE. Then the second colonoscopist observed the sigmoid colon and rectum. Each colonoscopist separately recorded the findings. The detection rate, miss rate and procedural time were assessed in prospective manner.The detection rate of flat and depressed adenoma, but not elevated adenoma, by AFI is significantly higher than that by HRE. In less-experienced endoscopists, AFI dramatically increased the detection rate (30.3%) and reduced miss rate (0%) of colorectal adenoma in comparison to those of HRE (7.7%, 50.0%), but not for experienced endoscopists. The procedural time of HRE was significantly shorter than that of AFI.AFI increased the detection rate and reduced the miss rate of flat and depressed adenomas. These advantages of AFI were limited to less-experienced endoscopists because experienced endoscopists exhibited a substantially high detection rate for colorectal adenoma with HRE.
Basolateral Mg2+ Extrusion via CNNM4 Mediates Transcellular Mg2+ Transport across Epithelia: A Mouse Model
Daisuke Yamazaki equal contributor,Yosuke Funato equal contributor,Jiro Miura,Sunao Sato,Satoru Toyosawa,Kazuharu Furutani,Yoshihisa Kurachi,Yoshihiro Omori,Takahisa Furukawa,Tetsuya Tsuda,Susumu Kuwabata,Shin Mizukami,Kazuya Kikuchi,Hiroaki Miki
PLOS Genetics , 2013, DOI: 10.1371/journal.pgen.1003983
Abstract: Transcellular Mg2+ transport across epithelia, involving both apical entry and basolateral extrusion, is essential for magnesium homeostasis, but molecules involved in basolateral extrusion have not yet been identified. Here, we show that CNNM4 is the basolaterally located Mg2+ extrusion molecule. CNNM4 is strongly expressed in intestinal epithelia and localizes to their basolateral membrane. CNNM4-knockout mice showed hypomagnesemia due to the intestinal malabsorption of magnesium, suggesting its role in Mg2+ extrusion to the inner parts of body. Imaging analyses revealed that CNNM4 can extrude Mg2+ by exchanging intracellular Mg2+ with extracellular Na+. Furthermore, CNNM4 mutations cause Jalili syndrome, characterized by recessive amelogenesis imperfecta with cone-rod dystrophy. CNNM4-knockout mice showed defective amelogenesis, and CNNM4 again localizes to the basolateral membrane of ameloblasts, the enamel-forming epithelial cells. Missense point mutations associated with the disease abolish the Mg2+ extrusion activity. These results demonstrate the crucial importance of Mg2+ extrusion by CNNM4 in organismal and topical regulation of magnesium.
Introducing a Function with Plural Derivatives  [PDF]
Changsoo Shin
Journal of Applied Mathematics and Physics (JAMP) , 2016, DOI: 10.4236/jamp.2016.43056
Abstract: It is a solid truth in mathematics that the derivative of a function is unique. We want to show that there exist particular functions all of which have the same form but their derivatives are different. Even though this may seem quite novel, such function could be crucial for the purpose of describing the world such as related to mental phenomena where the logic of the current mathematics is not adequate.
Possible Effect of Pressure Solution on the Movement of a Canister in the Buffer of Geological Disposal System  [PDF]
Koichi Shin
International Journal of Geosciences (IJG) , 2017, DOI: 10.4236/ijg.2017.82006
Abstract: One of the major concepts of the geological disposal of high level radioactive waste is to enclose a metallic container with bentonite buffer which is considered to be impermeable and chemically stable. Since the average density of the container is around 6 to 7 and very heavy compared to bentonite, the scenario of container sinking has been evaluated because excess sinking makes short the pathway of nuclide migration in the bentonite and is detrimental to the safety of the disposal system. Previous considerations on container sinking have been made from the viewpoint of mechanical deformation of the bentonite. In this paper, a chemical deformation process is presented as another mechanism of container sinking, which has not been previously considered for the container sinking in the field of radioactive waste disposal. The chemical deformation mentioned in this paper is the deformation through the process of pressure solution of minerals constituting the buffer, transportation by diffusion and precipitation. That such chemical deformation is a ubiquitous phenomenon occurring in various scales in the crust of the earth will be shown through the review of previous works. Then, some future research topics will be suggested which would be required in order to evaluate the container sinking in the safety case for radioactive waste disposal.
“Passive-bending colonoscope” significantly improves cecal intubation in difficult cases
Takeshi Mizukami,Haruhiko Ogata,Toshihumi Hibi
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i32.4454
Abstract: Colonoscopy sometimes causes pain during insertion, especially in difficult cases. Over-insufflation of air causes elongation or acute angulations of the colon, making passage of the scope difficult and causing pain. We previously reported a sedative-risk-free colonoscopy insertion technique, namely, “Water Navigation Colonoscopy”. Complete air suction after water infusion not only improves the vision, but also makes water flow down to the descending colon, while the sigmoid colon collapses and shortens. While non-sedative colonoscopy can be carried out without pain in most cases, some patients do complain of pain. Most of these patients have abnormal colon morphology, and the pain is caused while negotiating the “hairpin” bends of the colon. The “hairpin” bends of the colon should be negotiated by gently pushing the full-angled colonoscope. The proximal 10-20 cm from the angulated part of the conventional colonoscope is stiff, with a wide turning radius, therefore, a conventional colonoscope cannot be negotiated through the “hairpin” bends of the colon without stretching them and causing pain. The “passive-bending colonoscope” has a flexible tip with a narrow turning radius, so that the scope can be negotiated through the “hairpin” bends of the colon with a minimum turning radius and minimal discomfort. Therefore, the intubation and pain-reducing performance of the “passive-bending colonoscope” was assessed in difficult cases.
Aprendizagem da docência: algumas contribui es de L. S. Shulman
Maria da Gra?a Nicoletti Mizukami
Educa??o : Revista do Centro de Educa??o UFSM , 2004,
Abstract: O que os professores precisam saber para poder ensinar e para que seu ensino possa conduzir as aprendizagens dos alunos? Como os professores aprendem a ensinar? Como professores constróem conhecimentos sobre o ensino? Muitos pesquisadores vêm estudando a base de conhecimento profissional para o ensino a partir de uma variedade de perspectivas teórico-metodológicas. S o apresentadas nesse texto algumas contribui es de L. S. Shulman referentes à compreens o de processos de aprendizagem profissional da docência. Esta escolha se justifica pelo fato das obras do autor, principalmente nas duas últimas décadas, terem influenciado tanto pesquisas como políticas de forma o e desenvolvimento profissional de professores. Partindo de análises referentes ao paradigma de pesquisa sobre ‘pensamento do professor' e ‘conhecimento do professor' realizadas por Shulman s o apresentados inicialmente dois modelos, o primeiro referente a conhecimentos da docência e o segundo ao processo pelo qual tais conhecimentos s o aprendidos ao longo de processos formativos e do exercício profissional: a base de conhecimento para o ensino e o processo de raciocínio pedagógico. Ancorados nesses dois modelos e no conceito de profiss o docente s o apresentados, na parte final, argumentos de Shulman em defesa da sistematiza o do corpo de conhecimento relacionado ao ensino sob forma de casos de ensino. Casos de ensino e métodos de casos s o considerados sob uma perspectiva formativa e investigativa. Palavras-chave: Forma o de professores. Casos de Ensino e Métodos de Casos. Desenvolvimento Profissional de Professores.
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