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Search Results: 1 - 10 of 8170 matches for " Shilpa Sharma "
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Gene Expression Profiling of Human c-Kit Mutant D816V  [PDF]
Shilpa Sharma, Gurudutta Gangenahalli
Journal of Cancer Therapy (JCT) , 2016, DOI: 10.4236/jct.2016.76046
Abstract: The tyrosine kinase receptor III, c-Kit/stem cell factor receptor and its ligand, human stem cell factor (huSCF) are the predominant regulator of mitogenesis in the hematopoietic stem and progenitor cells. However, gain-of-function mutations alter c-Kit auto-regulatory mechanisms to aberrant c-Kit signaling, leading to the onset or progression of cancerous transformations. The most common mutation of c-Kit is the substitution of aspartic acid residue in position 816 to valine (D816V), which is majorly responsible for its ligand-independent constitutive activation, and is implicated in hematopoietic malignancies. Currently, molecular targeted therapy is increasingly becoming a hot spot due to its specificity and low toxicity. As the molecular mechanisms responsible for D816V-c-Kit mediated tumorogenicity are largely unknown, in this study, we aimed to investigate the D816V-c-Kit signaling mediated downstream molecular targets. Specifically, we created c-Kit active mutant form D816V and performed inducible gene expression of mutant D816V-c-Kit in monomyelocytic cell line U937. Mutant D816V-c-Kit expressing cells revealed significantly enhanced cellular mitogenic activity compared to wild-type c-Kit expressing cells independent of huSCF. To examine the molecular targets regulating tumorogenic proliferation, we evaluated the consequences of mutant D816V-c-Kit expression on downstream gene expression profile by high throughput microarray technology. The levels of some of the relevant genes (PIK3CB, eIF4B, PRKCDBP, MOAP1) were validated by quantitative polymerase chain reaction. SLA, STAT5B, MAP3K2 and MAPK14 emerged as important downstream molecular targets of mutant D816V-c-Kit. Further, by dissecting the signaling pathways, we also demonstrated that the D816V-c-Kit mediated hematopoietic cell proliferation is dependent on molecular target p38 MAP kinase.
A Novel Technique of Skeletonization for Feature Extraction in Cadastral Maps
Shilpa Sharma,Prabhakar Sharma
International Journal of Electronics and Computer Science Engineering , 2012,
Abstract: A cadastral map is a map showing the boundaries and ownership of land parcels. The British evolved thepresent cadastral system for the purposes of governance and revenue collection. Cadastral maps can be treated as base maps for all land related information. Typically, these maps store information pertaining to ownership details, land use, areal extents, type of development that has been made by the parcel owner, topological relationship with neighboring plots etc. This paper presents a novel method for the extraction of various features of cadastral maps. The extraction process works in two phases. In the first phase, boundary pixels of the cadastral map are found and skeletonization is carried out on them. In the second phase, the manipulated data obtained from the previous phase is analyzed to extract the features of the given cadastral map. The cadastral maps are generally not very accurate. But most of the available skeletonization algorithms are very sensitive to inaccuracies. In the proposed method, the skeletonization algorithm has been modified to take care of the inherent inaccuracies present in the cadastral maps.
Improved Enhanced Chain Based Energy Efficient Wireless Sensor Network  [PDF]
Shilpa Mahajan, Jyoteesh Malhotra, Sandeep Sharma
Wireless Sensor Network (WSN) , 2013, DOI: 10.4236/wsn.2013.54011
Abstract:

In this paper, we have proposed an energy efficient chain based protocol which is an improvement over ECBSN (Energy Efficient Chain Based Sensor Network). ECBSN protocol has certain deficiencies like the non optimal selection of leader nodes in rounds, aggregation and transmission of data by head nodes that leads to unbalanced energy consumption. Aiming at these problems, an improved chain based protocol is proposed. IECBSN adopts a new method of selection of leader nodes based on selection value (SV) parameter .To lower energy consumption further, one more level of hierarchy has been added with a head leader node, which will aggregate data from the leader nodes and pass it to the base station. IECBSN shows an improvement of 20% - 35% as compare to PEGASIS (Power Efficient Gathering in Sensor Information System) and 5% t to 7% from ECBSN on energy consumption and improves network lifetime.

Modified Method for Denoising the Ultrasound Images by Wavelet Thresholding
Alka Vishwa,Shilpa Sharma
International Journal of Intelligent Systems and Applications , 2012,
Abstract: Medical practitioners are increasingly using digital images during disease diagnosis. Several state-of-the-art medical equipment are producing images of different organs, which are used during various stages of analysis. Examples of such equipment include MRI, CT, ultrasound and X-Ray. In medical image processing, image denoising has become a very essential exercise all through the diagnosis as Ultrasound images are normally affected by speckle noise. The noise in the image has two negative outcomes, the first being the degradation of the image quality and the second and more important, obscures important information required for accurate diagnosis.Arbitration between the perpetuation of useful diagnostic information and noise suppression must be treasured in medical images. In general we rely on the intervention of a proficient to control the quality of processed images. In certain cases, for instance in Ultrasound images, the noise can suppress the information which is valuable for the general practitioner. Consequently medical images can be very inconsistent, and it is crucial to operate case to case. This paper presents a wavelet-based thresholding scheme for noise suppression in Ultrasound images and provides the knowledge about adaptive and anisotropic diffusion techniques for speckle noise removal from different types of images, like Ultrasound.
An Ontology Based Reuse Algorithm towards Process Planning in Software Development
Shilpa Sharma,Maya Ingle
International Journal of Advanced Computer Sciences and Applications , 2011,
Abstract: The process planning task for specified design provisions in software development can be significantly developed by referencing the knowledge reuse scheme. Reuse is considered to be one of the most promising techniques to improve software excellence and productivity. Reuse during software development depends much on the existing design knowledge in meta-model, a “read only” repository of information. We have proposed, an ontology based reuse algorithm towards process planning in software development. According to the common conceptual base facilitated by ontology and the characteristics of knowledge, the concepts and the entities are represented into meta-model and endeavor prospects. The relations between these prospects and its linkage knowledge are used to construct an ontology based reuse algorithm. In addition, our experiment illustrates realization of process planning in software development by practicing this algorithm. Subsequently, its benefits are delineated.
An Ontology Driven Information System
Shilpa Sharma,Maya Ingle
International Journal of Computer Technology and Applications , 2011,
Abstract: In the era of coalescing application landscapes a big potential for defining, implementing, disseminating and using ontologies is evolving. Whenever a software development project is not just concerned with a specific application but is part of a landscape of projects located in different domains, an ontology-driven approach may be worth considering and even be advantageous as compared to a traditional approach. One principal goal of this approach is to extend the idea of reuse from the modelling to implementation level. Instead of building systems right from the scratch or from the ready-made components which are plugged together such as hardware modules, ontologies are reusable model components from which particular implementations can be derived for any domain specific application . In this paper, the conceptual modeling is practiced as an Object Oriented Software Engineering and is mapped with the ontology approach to devise Ontology Driven Software Engineering approach. The resultant life cycle models various Ontology Driven Information Systems from different domains such as Expert Systems, Decision Support Systems and Process Control Systems etc. Finally, some perspectives of an Ontology Driven Software Engineering approach are outlined.
Congenital pouch colon - Then and now
Gupta Devendra,Sharma Shilpa
Journal of Indian Association of Pediatric Surgeons , 2007,
Abstract: Congenital pouch colon (CPC), a condition associated with anorectal agenesis has been reported mainly from the Indian subcontinent though there have been few case reports from other areas. The pouch differs from a normal colon structurally, histologically and functionally. The management involves a diversion colostomy at birth with or without the excision of pouch followed by pull through. This review article attempts to compile all the available literature on pouch colon along with the authors′ experience and discuss the relevant issues for proper diagnosis and management. A changing trend has been seen in the most common type of CPC seen over the years from complete congenital pouch colon that accounted for more than 70% of cases earlier to incomplete pouch colon that is more commonly seen now.
Editorial comments
Gupta D,Sharma Shilpa
Journal of Indian Association of Pediatric Surgeons , 2005,
Abstract:
Management of empyema - Role of a surgeon
Gupta D,Sharma Shilpa
Journal of Indian Association of Pediatric Surgeons , 2005,
Abstract: Postpneumonic empyema still remains quite common in developing countries, especially during the hot and humid months. While most cases would respond to antibiotic therapy, needle aspiration and intercostal drainage, few cases require further surgical management. The most common nontubercular etiological agent is Staphylococcus. Tubercular etiology is not uncommon in India, especially due to delayed presentation, multiresistant strains, mismanaged cases, and noncompliance with antitubercular treatment amidst malnutrition and anemia. Clinical symptoms, a skiagram chest followed by thoracentesis are enough for diagnosis. Pleural fluid is usually diagnostic and helps in choosing the appropriate antibiotics. Further investigations and management depends on the stage of the disease. Thoracentesis alone may be sufficient for the exudative phase. In fibrinopurulent stage, a properly sized and well-placed tube thoracostomy with underwater seal is curative in most cases. Interventional radiologists have placed small-bore catheters, specifically directed to the loculated collection and have used fibrinolytics like urokinase, streptokinase, and tissue plasminogen activator (TPA) to break loculations, ameliorate fibrous peel formation, and fibrin deposition. Thoracoscopic debridement and thoracoscopic decortication is an alternative with distinct advantages over thoracotmy and are indicated if there was no response with intercostal drainage procedure. In the organizing stage, a thoracotomy (for decortication) would be required if there is a loculated empyema, underlying lung disease or persistently symptomatic effusions. Timely institution of proper management prevents the need for any surgical intervention and avoids long-term morbid complications.
Stem cell therapy - Hope and scope in pediatric surgery
Gupta Devendra,Sharma Shilpa
Journal of Indian Association of Pediatric Surgeons , 2005,
Abstract: A stem cell is an undifferentiated cell in the body with undetermined function capable of forming various tissues under definite signals received from the body. Stem cell research in animals using embryonal stem cells has been an ongoing program in the west with fruitful results. However, only limited information is available with the use of stem cells in human beings. Of the various sources of stem cells, umbilical cord blood stem cell research has shown potential for future treatment in Alzheimer′s, Parkinson′s, heart attack, stroke and spinal cord injuries. Human trials have been done in diseases like spinal cord injury and chronic liver cirrhosis. Cord blood stem cells have already been effectively used in the treatment of sickle cell, leukemia, non-Hodgkin′s lymphoma and some other cancers, life threatening anemias and auto-immune diseases. Current challenges with the use of stem cells in clinical practice include the provisions to direct the differentiation of embryonic stem cells into specialized cell populations, and also devise ways to guard their development or proliferation once placed in vivo. Only further research and its clinical application will solve the many unanswered queries.
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