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Sodium azide (NaN3)
(2 mM) was used to induce
mutation in black soybean variety CRWD for improvement of isoflavone content.
The individual selections were made in M6, M7 and M8 generations, respectively.
Both high levels of isoflavones and medium seed size traits were used as
selection criteria. Across three growth seasons, the selected five NaN3-induced
mutants accumulated more total
isoflavones (average increases of 5% to 25%) than their wild type variety CRWD. Four mutants yielded
more (average increases of 17% to 25%) than CRWD. Both mutants SA-M-03 (yellow cotyledon) and SA-M-05 (green cotyledon) are rich in isoflavones
and may be useful in food and other applications.