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Search Results: 1 - 10 of 103576 matches for " Shaoyan Zhang "
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Network Anomaly Detection Using One Class Support Vector Machine
Rui Zhang,Shaoyan Zhang,Yang Lan,Jianmin Jiang
Lecture Notes in Engineering and Computer Science , 2008,
RNA Interference Targeting Slug Increases Cholangiocarcinoma Cell Sensitivity to Cisplatin via Upregulating PUMA
Kejun Zhang,Dong Chen,Xingang Wang,Shaoyan Zhang,Jigang Wang,Yuan Gao,Bomin Yan
International Journal of Molecular Sciences , 2011, DOI: 10.3390/ijms12010385
Abstract: Slug is an E-cadherin repressor and a suppressor of PUMA (p53 upregulated modulator of apoptosis) and it has recently been demonstrated that Slug plays an important role in controlling apoptosis. In this study, we examined whether Slug’s ability to silence expression suppresses the growth of cholangiocarcinoma cells and/or sensitizes cholangiocarcinoma cells to chemotherapeutic agents through induction of apoptosis. We targeted the Slug gene using siRNA (Slug siRNA) via full Slug cDNA plasmid (Slug cDNA) transfection of cholangiocarcinoma cells. Slug siRNA, cisplatin, or Slug siRNA in combination with cisplatin, were used to treat cholangiocarcinoma cells in vitro. Western blot was used to detect the expression of Slug, PUMA, and E-cadherin protein. TUNEL, Annexin V Staining, and cell cycle analysis were used to detect apoptosis. A nude mice subcutaneous xenograft model of QBC939 cells was used to assess the effect of Slug silencing and/or cisplatin on tumor growth. Immunohistochemical staining was used to analyze the expression of Slug and PUMA. TUNEL was used to detect apoptosis in?vivo. The results showed that PUMA and E-cadherin expression in cholangiocarcinoma cells is Slug dependent. We demonstrated that Slug silencing and cisplatin both promote apoptosis by upregulation of PUMA, not by upregulation of E-cadherin. Slug silencing significantly sensitized cholangiocarcinoma cells to cisplatin through upregulation of PUMA. Finally, we showed that Slug silencing suppressed the growth of QBC939 xenograft tumors and sensitized the tumor cells to cisplatin through PUMA upregulation and induction of apoptosis. Our findings indicate that Slug is an important modulator of the therapeutic response of cholangiocarcinoma cells and is potentially useful as a sensitizer in cholangiocarcinoma therapy. One of the mechanisms is the regulation of PUMA by Slug.
Slug down-regulation by RNA interference inhibits invasion growth in human esophageal squamous cell carcinoma
Peng Tang, Zhentao Yu, Kejun Zhang, Yu Wang, Zhongliang Ma, Shaoyan Zhang, Dong Chen, Yanbing Zhou
BMC Gastroenterology , 2011, DOI: 10.1186/1471-230x-11-60
Abstract: We utilized RNA interference to knockdown Slug gene expression, and effects on survival and invasive carcinoma were evaluated using a Boyden chamber transwell assay in vitro. We evaluated the effect of Slug siRNA-transfection and Slug cDNA-transfection on E-cadherin and Bcl-2 expression in ESCC cells. A pseudometastatic model of ESCC in immunodeficient mice was used to assess the effects of Slug siRNA transfection on tumor metastasis development.The EC109 cell line was transfected with Slug-siRNA to knockdown Slug expression. The TE13 cell line was transfected with Slug-cDNA to increase Slug expression. EC109 and TE13 cell lines were tested for the expression of apoptosis-related genes bcl-2 and metastasis-related gene E-cadherin identified previously as Slug targets. Bcl-2 expression was increased and E-cadherin was decreased in Slug siRNA-transfected EC109 cells. Bcl-2 expression was increased and E-cadherin was decreased in Slug cDNA-transfected TE13 cells. Invasion of Slug siRNA-transfected EC109 cells was reduced and apoptosis was increased whereas invasion was greater in Slug cDNA-transfected cells. Animals injected with Slug siRNA-transfected EC109 cells exhihited fewer seeded nodes and demonstrated more apoptosis.Slug down-regulation promotes cell apoptosis and decreases invasion capability in vitro and in vivo. Slug inhibition may represent a novel strategy for treatment of metastatic ESCC.Despite improvements in detection, surgical resection, and (neo-) adjuvant therapy, the overall survival for esophageal squamous cell carcinoma (ESCC), one of the most aggressive carcinomas of the gastrointestinal tract, remains lower than that of other solid tumors due to distant metastasis [1]. Therefore, efforts are ongoing regarding exploration of novel targets and strategies for the management of ESCC, and gene targeting therapies in particular are promising.Multiple studies focusing on the effects of various biological factors on the malignant potential of ESCC have
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Shiwu Zhang, Lisha Qi, Man Li, Danfang Zhang, Shaoyan Xu, Ning Wang, Baocun Sun
Journal of Experimental & Clinical Cancer Research , 2008, DOI: 10.1186/1756-9966-27-62
Abstract: The distribution and expression of CXCL12, CXCR4, MMP-2 and MMP-9 in human prostate cancer and in tumor cells invading nerve tissue were studied with immunohistochemical staining. The effects of exogenous CXCL12 and CXCR4 antagonist AMD3100 on PC3 prostate cancer cells invasiveness were assessed in vitro and in vivo.The expression of CXCL12, CXCR4, MMP-2, and MMP-9 in human prostate cancer were higher than those in hyperplastic prostate tissues (P < 0.05). In vitro CXCL12 could stimulate the PC3 cells invasiveness (P < 0.05) while AMD3100 could inhibit invasiveness. In vivo, the number of nerves around the tumor tissue in the group treated with CXCL12 was significantly higher than that found in the control group (P < 0.05). Both the control group and the CXCL12-treated group had more nerves number near the tumor tissue than it found in the AMD3100-treated group. The positive cell number of CXCL12, CXCR4, MMP-2, MMP-9, and NGF expression ranked from highest to lowest, were the CXCL12-treated, the control, and the AMD3100-treated group(P < 0.05).CXCL12 and its receptor CXCR4 along with MMP-2 and MMP-9 are related with prostate cancer perineural invasion.Perineural invasion (PNI) of malignant tumor cells means tumor cells tend to encroach upon nerve fibers and invade the lamellar sheath, leading to infiltration and metastasis. This phenomenon is often seen in adenoid cystic carcinoma, pancreatic cancer and prostate cancer [1]. Prostate cancer PNI usually localizes in a few important nerves, influencing the patients' quality of life and making it difficult to remedy. Residual tumor cells in nerve tissue may contribute to local recurrence and metastasis and influence prognosis [2]. Other studies have shown that the mechanism of PNI involves the microenvironment around the nerve tract that is suitable for tumor cell growth and proliferation. This situation promotes tumor cell infiltration and diffusion along nerve fibers [3].Chemokines are small, secreted peptides that co
Methylation and Expression of Retinoblastoma and Transforming Growth Factor- 1 Genes in Epstein-Barr Virus-Associated and -Negative Gastric Carcinomas
Xia Liu,Xiuming Tang,Shaoyan Zhang,Yun Wang,Xiaofeng Wang,Chengquan Zhao,Bing Luo
Gastroenterology Research and Practice , 2012, DOI: 10.1155/2012/906017
Abstract: Background. Retinoblastoma (RB) and transforming growth factor-β1 (TGF-β1) are important tumor-related factors. Methods. A series of 30 EBV-associated gastric carcinoma (EBVaGC) and 38 matched EBV-negative gastric carcinoma (EBVnGC) tissues were examined for the promoter methylation of RB by methylation-specific PCR (MSP) method. The expression of RB and TGF-β1 in gastric carcinoma tissues was detected by immunohistochemistry. Results. The methylation rate of RB gene in EBVaGC and EBVnGC was 80.0% (24/30) and 50.0% (19/38), respectively. The difference of RB methylation rate between EBVaGC and EBVnGC was significant (2=6.490,=0.011). There was no significant difference for RB expression between EBVaGC (43.3%, 13/30) and EBVnGC (63.2%, 24/38), and also for TGF-β1 between EBVaGC (56.7%, 17/30) and EBVnGC (63.2%, 24/38). RB methylation was not reversely correlated with RB expression in gastric carcinoma tissues (2=2.943,=0.086,=0.208). RB methylation, loss expression of RB, and TGF-β1 expression were significantly associated with tumor invasion and lymph node metastasis (<0.05), but was not associated with sex, age, histological subtype (differentiation status) and tumor location. Conclusions. Methylation of RB is a common event in gastric carcinomas and EBV induces methylation of RB in EBVaGC, which may contribute to the development of gastric carcinomas. EBV has no significant effect on induction of TGF-β1 expression. Detection of RB methylation, RB expression, and TGF-β1 expression may be helpful to judge the status of tumor invasion and lymph node metastasis in gastric carcinomas.
Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma
Shiwu Zhang, Man Li, Yanjun Gu, Zhiyong Liu, Shaoyan Xu, Yanfeng Cui, Baocun Sun
Journal of Experimental & Clinical Cancer Research , 2008, DOI: 10.1186/1756-9966-27-60
Abstract: Thirty mice inoculated subcutaneously with B16F10 cells were randomly divided into the treatment group and the control group. Thalidomide was administered once a day at a dose of 200 mg/kg for the treatment group starting on the fifth day after inoculation, and an equivalent volume of 0.5% carboxylmethyl cellulose was administered similarly in the control group. The diameter of the tumors was measured daily after inoculation until the mice were sacrificed on the 19th day. The different blood supply patterns were counted after immunohistochemical and PAS histochemical double-Staining. VEGF, NF-κB, PCNA, MMP-2 and MMP-9 expression in tumor tissue was also assessed.The tumor volume(P = 0.019) and the number of vasculogenic mimicry(P = 0.03) and mosaic vessels(P = 0.004) in the treatment group were significantly decreased compared with the control group. VEGF(P = 0.004), NF-κB(P = 0.009), PCNA(P = 0.002), MMP-2 (P = 0.000), MMP-9(P = 0.002) protein expression and MMP-2(P = 0.000) and MMP-9(P = 0.000) mRNA in the treatment group were significantly lower than those in the control groups.Thalidomide inhibits vasculogenic mimicry channel and mosaic vessels formation in melanoma through the regulation of vasculogenic factors, and it can induce necrosis of melanoma cells, which may be related with the NF-κB signaling pathway.Thalidomide was first introduced in the late 1950s for the prevention of morning sickness in pregnant women, but it was withdrawn from the market in the 1960s because of its well-known teratogenicity[1]. Recently thalidomide has been found to have anti-angiogenesis and anti-inflammatory properties, and based on these observations thalidomide has been used as a therapeutic reagent in some malignant tumor including liver cancer, renal cell carcinoma, breast cancer and so on[2]. Many studies also focused on the effects of thalidomide on metastatic melanoma. The mechanism of thalidomide against melanoma maybe attribute to its anti-angiogenic activity. However
Differential expression of decorin, EGFR and cyclin D1 during mammary gland carcinogenesis in TA2 mice with spontaneous breast cancer
Yanjun Gu, Shiwu Zhang, Qiang Wu, Shaoyan Xu, Yanfen Cui, Zhengduo Yang, Xiulan Zhao, Baocun Sun
Journal of Experimental & Clinical Cancer Research , 2010, DOI: 10.1186/1756-9966-29-6
Abstract: Gene expression profiles of spontaneous breast cancer and matched normal mammary gland tissues in TA2 mice were ascertained using an Affymetrix Mouse 430 2.0 array. Twelve mammary tissue samples from five month-old female TA2 mice (Group A), as well as 28 samples from mammary (Group B) and cancer tissues (Group C) of spontaneous breast cancer-bearing TA2 mice, were subsequently used to detect the expression of decorin, EGFR and cyclin D1 by real-time PCR and immunohistochemical methods.Several imprinted genes, oncogenes and tumor suppressor genes were differentially expressed between normal mammary gland tissues and breast cancer tissues of TA2 mice. The imprinted gene decorin and the oncogene EGFR were down-regulated in tumor tissues, while the oncogene cyclin D1 was up-regulated. Immunohistochemistry showed that samples in Group A showed high decorin expression more frequently than those in Group B (P < 0.05). More tissue samples in Group B than Group A were positive for nuclear EGFR, and tissue samples in Group B more frequently showed high nuclear EGFR expression than those in Group A or Group C (P < 0.05). The labeling index for cyclin D1 in Group C was significantly higher than in Group B. Mammary tissues of Group A expressed the highest level of decorin mRNA (P < 0.05), and mammary tissues of Group B expressed the highest level of EGFR mRNA (P < 0.05), while cancer tissues expressed the highest level of cyclin D1 mRNA (P < 0.05).The expression of decorin, EGFR and cyclin D1 in mammary epithelial cells changes with increasing age. The abnormal expression of them may partly contribute to the genesis of spontaneous breast cancer in TA2 mice.The Tientsin Albino 2 (TA2) mouse is an inbred strain originating from the Kunming strain. It has a high incidence of spontaneous breast cancer without the need for external inducers or carcinogens. The morbidity in parous females is 84.1% within an average of 280 days after birthing a litter [1-3]. Until now, the mechanism o
Control of epitaxial relationships of ZnO/SrTiO3 heterointerfaces by etching the substrate surface
Caihong Jia,, Yonghai Chen, Xianglin Liu, Shaoyan Yang, Weifeng Zhang and Zhanguo Wang
Nanoscale Research Letters , 2013, DOI: 10.1186/1556-276X-8-23
Abstract: Wurtzite ZnO thin films with different epitaxial relationships are obtained on as-received and etched (001), (011), and (111) SrTiO3 (STO) by metal-organic chemical vapor deposition (MOCVD). ZnO films exhibit nonpolar (1120) orientation with in-plane orientation relationship of <0001>ZnO//<110>STO on as-received (001) STO, and polar c-axis growth with <1100>ZnO//<110>STO on etched (001) STO substrates. ZnO films change from polar (0001) to semipolar (1012) oriented on as-received and etched (011) STO. On as-received and etched (111) STO, ZnO films show the same growing direction of polar (0001), but different in-plane orientations with 30° rotation. The change of epitaxial relationship of ZnO films on as-received and etched (001), (011), and (111) STO substrates is accompanied with the increase of lattice mismatch, decrease of bond density, and increase of substrate surface roughness. In other words, the epitaxial relationships of ZnO/STO heterointerfaces can be controlled by etching the substrates. These results show that polar, nonpolar, and semipolar ZnO films for different applications can be grown epitaxially on STO substrates by MOCVD.

WU Shaoyan,ZHANG Qingfu,CHEN Huowang,

软件学报 , 1997,
Abstract: Several popular approaches of simulated evolution have been developed separately. These approaches emphasize different facets of the natural evolutionary processes, respectively. One has recognized that the simulated evolution will benefit from the adequate combination between the approaches. This paper characterizes the primary difference among existing approaches as the difference between genetic link and behavioral link. A new model of simulated evolution, called FEBE(family eugenics based evolution), is proposed, which combines the genetic link with the behavioral link in light of the idea of family eugenics. In the FEBE model the orthogonal design technique is introduced into offspring's breeding inside a family so as to enhance the behavioral improvement of individuals. The FEBE model is applied to solve Goldberg's deceptive problem that is challenging to most evolutionary algorithms. The exciting experimental results are achieved.
Room-temperature ferromagnetic semiconductor Mn x Ga1 x Sb
Nuofu Chen,Fuqiang Zhang,Junling Yang,Zhikai Liu,Shaoyan Yang,Chunlin Chai,Zhanguo Wang,Wenrui Hu,Lanying Lin
Chinese Science Bulletin , 2003, DOI: 10.1360/03tb9109
Abstract: Ferromagnetic semiconductor Mn x Ga1 x Sb single crystals were fabricated by Mn-ions implantation, deposition, and the post annealing. Magnetic hysteresis-loops in the Mn x Ga1 x Sb single crystals were obtained at room temperature (300 K). The structure of the ferromagnetic semiconductor MnxGa1 x Sb single crystal was analyzed by X-ray diffraction. The distribution of carrier concentrations in Mn x Ga1 x Sb was investigated by electrochemical capacitance-voltage profiler. The content of Mn in Mn x Ga1 x Sb varied gradually from x = 0.09 near the surface to x = 0 in the wafer inner analyzed by X-ray diffraction. Electrochemical capacitance-voltage profiler reveals that the concentration of p-type carriers in Mn x Ga1 x Sb is as high as 1× 1021 cm 3, indicating that most of the Mn atoms in Mn x Ga1 x Sb take the site of Ga, and play a role of acceptors.
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