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Search Results: 1 - 10 of 34450 matches for " Shaoxia Zhou "
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Interaction of Stellate Cells with Pancreatic Carcinoma Cells
Hansj?rg Habisch,Shaoxia Zhou,Marco Siech,Max G. Bachem
Cancers , 2010, DOI: 10.3390/cancers2031661
Abstract: Pancreatic cancer is characterized by its late detection, aggressive growth, intense infiltration into adjacent tissue, early metastasis, resistance to chemo- and radiotherapy and a strong “desmoplastic reaction”. The dense stroma surrounding carcinoma cells is composed of fibroblasts, activated stellate cells (myofibroblast-like cells), various inflammatory cells, proliferating vascular structures, collagens and fibronectin. In particular the cellular components of the stroma produce the tumor microenvironment, which plays a critical role in tumor growth, invasion, spreading, metastasis, angiogenesis, inhibition of anoikis, and chemoresistance. Fibroblasts, myofibroblasts and activated stellate cells produce the extracellular matrix components and are thought to interact actively with tumor cells, thereby promoting cancer progression. In this review, we discuss our current understanding of the role of pancreatic stellate cells (PSC) in the desmoplastic response of pancreas cancer and the effects of PSC on tumor progression, metastasis and drug resistance. Finally we present some novel ideas for tumor therapy by interfering with the cancer cell-host interaction.
A Novel Low Temperature PCR Assured High-Fidelity DNA Amplification
Guofan Hong,Sin Hang Lee,Shichao Ge,Shaoxia Zhou
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140612853
Abstract: As previously reported, a novel low temperature (LoTemp) polymerase chain reaction (PCR) catalyzed by a moderately heat-resistant (MHR) DNA polymerase with a chemical-assisted denaturation temperature set at 85 °C instead of the conventional 94–96 °C can achieve high-fidelity DNA amplification of a target DNA, even after up to 120 PCR thermal cycles. Furthermore, such accurate amplification is not achievable with conventional PCR. Now, using a well-recognized L1 gene segment of the human papillomavirus (HPV) type 52 (HPV-52) as the template for experiments, we demonstrate that the LoTemp high-fidelity DNA amplification is attributed to an unusually high processivity and stability of the MHR DNA polymerase whose high fidelity in template-directed DNA synthesis is independent of non-existent 3'–5' exonuclease activity. Further studies and understanding of the characteristics of the LoTemp PCR technology may facilitate implementation of DNA sequencing-based diagnostics at the point of care in community hospital laboratories.
Neuroprotective effect of panax notoginseng saponins and its main components  [PDF]
Xumei Wang, Shaoxia Wang, Limin Hu
World Journal of Neuroscience (WJNS) , 2014, DOI: 10.4236/wjns.2014.41002
Abstract:

Stroke is the third leading cause of death and the first cause of adult disability in industrial countries [1]. It is charicaterized by hemiplegia, hemianopsia, aphasia, mouth askew and sever sequelae. It is considered that an ischemic disease without any specific treatment method and few effective drugs such as tPA (human tissue-type plasminogen activator) and Edarovone with specific therapeutic window will cause a lot of disadvantages if being used inaccurate. Root of Panax notoginseng (PN) which is one of traditional Chinese medicines (TCMs), was first found in “Shennong’s Classic of Materia Medica” around 200 AD. Panax notogineng saponins(PNS) is a multi-components mixture containing ginseng and saponins as the most important bioactive components which are commonly used in clinical treatment. Also, ginseng and saponins form the main components of many herbal medicines in the market, e.g., Xueshuantong injection [2], Xuesaitong injection [3], Xuesaitong soft capsule [4] and so on. The main monomers of Panax notoginseng saponins (PNS) are Ginsenoside-Rb1, Gensenoside-Rg1, Gensenoside-Re, Gensenoside-Rd and Panax notoginseng saponins-R1 [5]. In this review, we found some important points as well as shortcomings that require special consideration. We therefore highlighted the advances in neuro-protection of PNS and its main monomers in the area of experimental research.

The screening of a flocculant-producing strain and optimizing of its culture conditions
一种高效微生物絮凝剂产生菌的筛选及培养基优化

ZHOU Li,ZHANG Yongkui,CHEN Xiao,LI Wanquan,XU Shaoxia,
周礼
,张永奎,陈晓,李万全,徐绍霞

环境科学学报 , 2006,
Abstract: 从某自来水处理厂的活性污泥中筛选得到了一株稳定高效的微生物絮凝剂产生菌MBF-33,所产絮凝剂对高岭土悬浮液体系有较好的絮凝作用.通过培养基优化,对高岭土的絮凝率从81.3%提高到95%.实验结果表明:(1)适宜的单一碳源为25 g·L-1葡萄糖;(2)复合碳源效果优于单一碳源,适宜的复合碳源为蔗糖5 g·L-1,葡萄糖20 g·L-1;(3)无机氮不利于该菌生长,适宜的氮源为单一有机氮,为1.5 g·L-1蛋白胨;(4)0.2 g·L-1的MgSO4有利于菌生长,但不利于絮凝剂产生.
Cloning and roles of goldfish maternal factorβ-Catenin cDNA in embryonic development
Jingpu Zhang,Weixian Wang,Shaoxia Zhu
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03184265
Abstract: Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. β-Catenin, an important maternal factor in early embryogenesis, has been analyzed in its expression pattern and functions in this paper. We have cloned goldfishβ-Catenin cDNA gene and compared it with zebrafishβ-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern ofβ-Catenin byin situ hybridization and the roles of β-Catenin on embryonic development by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that β-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of β-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposterior axes, and leads embryos to die before larva get hatched.
Cloning and roles of goldfish maternal factorβ-Catenin cDNA in embryonic development
Cloning and roles of goldfish maternal factor β-Catenin cDNA in embryonic development

ZHANG Jingpu,WANG Weixian & ZHU Shaoxia,
ZHANGJingpu
,WANGWeixian,ZHUShaoxia

科学通报(英文版) , 2004,
Abstract: Interaction between nucleus and cytoplasm has been focused in the field of animal embryonic development, in which study of maternal factors is required positively. b-Catenin, an important maternal factor in early embryo-genesis, has been analyzed in its expression pattern and func-tions in this paper. We have cloned goldfish b-Catenin cDNA gene and compared it with zebrafish b-Catenin cDNA. High homology was found in cDNA and in amino acid sequences between them, 93% (2227/2384 bp) and 98.5% (768/780 aa) respectively. The expression pattern of b-Catenin by in situ hybridization and the roles of b-Catenin on embryonic de-velopment by co-injection of anti-sense RNA and reporter gene, EGFP have been investigated in the whole process of goldfish embryonic development. The results suggest that b-Catenin presents dynamic distribution, mainly locates at body axis, dorsal tissues, head and tail structures after being fertilized. The loss of b-Catenin activity would cause serious destruction of embryo in dorsal tissues and in anteroposte-rior axes, and leads embryos to die before larva get hatched.
印度尼西亚群岛邻近海域SST对越赤道气流的影响
刘秦玉 Liu Qinyu,王韶霞 Wang Shaoxia
大气科学 , 2000, DOI: 10.3878/j.issn.1006-9895.2000.05.07
Abstract: 利用COADS资料研究了印度尼西亚群岛邻近海域海表面温度(SST)的季节变化特征,并根据热带大气扰动量的低阶模式,分析了印度尼西亚群岛邻近SST的季节变化对越赤道气流季节变化的影响.研究结果表明:印度尼西亚群岛邻近海域年平均SST基本上关于赤道对称分布,最高温度出现在赤道附近;由于南海SST冬季较低,冬季(北半球)SST的经向差最大值(大于2℃)出现在110°E附近;夏季(北半球)最大经向温差出现在130~145°E和110°E附近,它有利于105°E越赤道气流的形成和维持.
Application of a nuclear localization signal gene in transgene mice
Jingpu Zhang,Yuge Wang,Guangsan Li,Yu Shen,Shaoxia Zhu,Miao Du
Chinese Science Bulletin , 2002, DOI: 10.1360/02tb9049
Abstract: Efficient gene transfer by cytoplasm co-injection will offer a powerful means for transgenic animals. Using co-injection in cytoplasm, two independent gene constructs, including bovine α-s1-casein-hG-CSF and a mammal expression vector expressing a nuclear localization signal (mNLS), were introduced into fertilized mouse eggs. The target gene construct was docked into host nucleus probably by the nuclear localization signal. Transgene mice have been obtained at 58% (29/50) of integration ratio. Expression level of the positive transgene mice was detected by Western blotting. Maximal expression of human G-CSF was estimated about 540 mg/L of milk. The expression ratio was up to 75% (9/12). The results here have important practical implications for the generation of mammary gland bioreactors and other transgene studies. Co-injection of a target gene with an expression vector of a mammal nuclear localization signal by cytoplasm appears to be a useful, efficient and easy strategy for generating transgenic animals, which may be able to substitute the routine method of pronucleus-injection of fertilized eggs.
Influence of the Sea Surface Temperature in the Vicinity of the Indonesia Archipelago on the Cross Equator Flow
印度尼西亚群岛邻近海域SST对越赤道气流的影响

Liu Qinyu,Wang Shaoxia,
刘秦玉
,王韶霞

大气科学 , 2000,
Abstract: 利用COADS资料研究了印度尼西亚群岛邻近海域海表面温度(SST)的季节变化特征,并根据热带大气扰动量的低阶模式,分析了印度尼西亚群岛邻近SST的季节变化对越赤道气流季节变化的影响.研究结果表明:印度尼西亚群岛邻近海域年平均SST基本上关于赤道对称分布,最高温度出现在赤道附近;由于南海SST冬季较低,冬季(北半球)SST的经向差最大值(大于2℃)出现在110°E附近;夏季(北半球)最大经向温差出现在130~145°E和110°E附近,它有利于105°E越赤道气流的形成和维持.
Application of a nuclear localization signal gene in transgene mice

ZHANG Jingpu,WANG Yuge,LI Guangsan,SHEN Yu,ZHU Shaoxia,DU Miao,

科学通报(英文版) , 2002,
Abstract: Efficient gene transfer by cytoplasm co-injec- tion will offer a powerful means for transgenic animals. Using co-injection in cytoplasm, two independent gene constructs, including bovine (?-s1-casein-hG-CSF and a mammal expression vector expressing a nuclear localization signal (mNLS), were introduced into fertilized mouse eggs. The target gene construct was docked into host nucleus probably by the nuclear localization signal. Transgene mice have been obtained at 58% (29/50) of integration ratio. Expression level of the positive transgene mice was detected by Western blotting. Maximal expression of human G-CSF was estimated about 540 mg/L of milk. The expression ratio was up to 75% (9/12). The results here have important practical implications for the generation of mammary gland bioreactors and other transgene studies. Co-injection of a target gene with an expression vector of a mammal nuclear localization signal by cytoplasm appears to be a useful, efficient and easy strategy for generating transgenic animals, which may be able to substitute the routine method of pronucleus-injection of fertilized eggs.
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