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Search Results: 1 - 10 of 32585 matches for " Shaoliang Huang "
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In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles
Ying Meng,Shaoliang Huang,Jun Min,Zhongmin Guo
Science China Life Sciences , 2006, DOI: 10.1007/s11427-006-0259-3
Abstract: Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
Induction of embryonic stem cells to hematopoietic cellsin vitro
Ling Xu,Chunping Qiao,Shaoliang Huang,Shunong Li,Xuan Wu
Chinese Science Bulletin , 2000, DOI: 10.1007/BF02898978
Abstract: In order to get hematopoietic cells from embryonic stem (ES) cells and to study development mechanisms of hematopoietic cells, the method of inducing embryonic stem cells to hematopoietic cells was explored by differenciating mouse ES cells and human embryonic cells in three stages. The differentiated cells were identified by flow cytometry, immunohistochemistry and Wright’s staining. The results showed that embryoid bodies (EBs) could form when ES cells were cultured in the medium with 2-mercaptoethanol (2-ME). However, cytokines, such as stem cell factor (SCF), thrombopoietin (TPO), interleukin-3 (IL-3), interleukin-6 (IL-6), erythropoietin (EPO) and granular colony stimulating factor (G-CSF), were not helpful for forming EBs. SCF, TPO and embryonic cell conditional medium were useful for the differentiation of mouse EBs to hematopoietic progenitors. Eighty-six percent of these cells were CD34+ after 6-d culture. Hematopoietic progenitors differentiated to B lymphocytes when they were cocultured with primary bone marrow stroma cells in the DMEM medium with SCF and IL-6. 14 d later, most of the cells were CD34-CD38+. Wright’s staining and immunohistochemistry showed that 80% of these cells were plasma-like morphologically and immunoglubolin positive. The study of hematopoietic cells from human embryonic cells showed that human embryonic cell differentiation was very similar to that of mouse ES cells. They could form EBs in the first stage and the CD34 positive cells account for about 48.5% in the second stage.
In vitro differentiation of mouse ES cells into hepatocytes with coagulation factors VIII and IX expression profiles

Ying Meng,Shaoliang Huang,Jun Min,Zhongmin Guo,

中国科学C辑(英文版) , 2006,
Abstract: Coagulation factors II, V, VII, VIII, IX and X are produced by hepatocytes. So factors VIII and IX deficiencies, which result in hemophilia A and B, have the potential to respond to cellular replacement therapy. Embryonic stem (ES) cells provide a unique source for therapeutic applications. Here, E14 mouse ES cells have been induced into hepatocytes in vitro. Morphology revealed that ES-derived hepatic-like cells were round or polyhedral shaped with distinct boundary of individual cells, and some arranged in trabeculae. These cells expressed endodermal-or liver-specific mRNA—transthyretin (TTR), α1-anti-trypsin (AAT), α-fetoprotein (AFP), albumin (ALB), glucose-6-phoshpatase (G6P) and tyrosine aminotransferase (TAT). Approximately (85.1±0.5)% of the ES-derived cells was stained positive green with ICG uptake. These cells were also stained magenta as a result of PAS reaction. In this paper, expression of coagulation factors VIII and IX mRNA in the ES-derived cells is documented. Therefore, ES cells might be developed as substitute donor cells for the therapy of coagulation factor deficiencies.
Experimental investigation on flow characteristics of deionized water in microtubes
ShaoLiang Xu,XiangAn Yue,JiRui Hou
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0118-z
Abstract: The flow characteristics of deionized water in microtubes with diameters ranging from 2 to 30 μm are investigated. The experimental results show that the flow characteristics in microtubes with diameters of 16 μm and larger ones are in agreement with the classical theory. However, as the diameters are decreased to 5 and 2 μm, the nonlinear flow characteristics prevail and the results indicate significant departure of flow characteristics from the predictions of the conventional theory, and the smaller the diameters, the larger the departure. As the Reynolds number increases, the degree of nonlinear flow characteristics decrease gradually and the experimental results are approximately equal to the theoretical expectation. The minimum Reynolds number in this study is only 2.46×10 5.
STUDIES ON DEVELOPMENT OF THERMOREGULATION IN MONGOLIAN GERBIL, MERIONES UGUICULATUS
长爪砂土鼠体温调节发育的研究

Jing Shaoliang,
景绍亮

生态学报 , 1982,
Abstract: 恒温动物体温调节发育的研究,具有重要的理论和实践意义,近几十年来有较多进展。但是,国内在啮齿动物方面尚未有过研究报道。 长爪砂土鼠(Meriones unguioulatus)分布于我国北部及蒙古的荒漠草原和干草原地带,是一种主要的草原害鼠(夏武平等,1966)。近二十多年来又成为广泛应用的实验动物,在国内外都有饲养种群(田嶋嘉雄,1972;Mele,1972)。关于长爪砂土鼠体温调节的发育,迄
catena-Poly[[chloridodimethyltin(IV)]-μ-chloroacetato-κ2O:O′]
Shaoliang Zhang,Junhong Zhang,Rufen Zhang
Acta Crystallographica Section E , 2011, DOI: 10.1107/s160053681104918x
Abstract: In the title polymeric coordination compound, [Sn(CH3)2(C2H2ClO2)Cl]n, the Sn atom has a distorted trigonal–bipyramidal geometry, with two O atoms of the ligands in axial positions and two methyl groups and one Cl atom in equatorial positions. Adjacent Sn atoms are bridged by the two O atoms of the carboxylate ligand, forming a chain structure along the a-axis direction.
Observation and simulation of flux rope structures at the dayside magnetopause
Hailong Cui,Shuping Jin,Shaoliang Liu,Zhenxing Liu,A. Balogh
Chinese Science Bulletin , 2003, DOI: 10.1360/03wd0258
Abstract: The signatures of flux ropes with obvious core magnetic field are detected by Cluster II at the dayside magnetopause during 11:00–11:15 UT on Mar. 2, 2001. The similar characteristics can be found from the magnetic field variations recorded by the four spacecrafts (Cluster II C1–C4). All the three ( /+) bipolar signatures in the B N component are accompanied with enhancements of B M and magnetic field strength B in the boundary normal coordinates (LMN coordinates). A MHD simulation with two dimensions and three components is performed to explore the reconnection process driven by the incoming flow of solar wind at the dayside magnetopause. The numerical results can illustrate the recurrent formation of magnetic structures with a core magnetic field. The time history of the magnetic field B and three components B x, By and B z at a given point of the current sheet can reproduce the observational features of the events mentioned above.
SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler
Ruibang Luo, Binghang Liu, Yinlong Xie, Zhenyu Li, Weihua Huang, Jianying Yuan, Guangzhu He, Yanxiang Chen, Qi Pan, Yunjie Liu, Jingbo Tang, Gengxiong Wu, Hao Zhang, Yujian Shi, Yong Liu, Chang Yu, Bo Wang, Yao Lu, Changlei Han, David W Cheung, Siu-Ming Yiu, Shaoliang Peng, Zhu Xiaoqian, Guangming Liu, Xiangke Liao, Yingrui Li, Huanming Yang, Jian Wang, Tak-Wah Lam, Jun Wang
GigaScience , 2012, DOI: 10.1186/2047-217x-1-18
Abstract: To overcome these challenges, we have developed its successor, SOAPdenovo2, which has the advantage of a new algorithm design that reduces memory consumption in graph construction, resolves more repeat regions in contig assembly, increases coverage and length in scaffold construction, improves gap closing, and optimizes for large genome.Benchmark using the Assemblathon1 and GAGE datasets showed that SOAPdenovo2 greatly surpasses its predecessor SOAPdenovo and is competitive to other assemblers on both assembly length and accuracy. We also provide an updated assembly version of the 2008 Asian (YH) genome using SOAPdenovo2. Here, the contig and scaffold N50 of the YH genome were ~20.9 kbp and ~22 Mbp, respectively, which is 3-fold and 50-fold longer than the first published version. The genome coverage increased from 81.16% to 93.91%, and memory consumption was ~2/3 lower during the point of largest memory consumption.The increased use of next generation sequencing (NGS) has resulted in an increased growth of the number of de novo genome assemblies being carried out using short reads. Although there are several de novo assemblers available, there remains room for improvement as shown in recent assembly evaluation projects such as Assemblathon 1 [1] and GAGE [2]. Since the publication of the first version of SOAPdenovo [3], it has been used to assemble many large eukaryotic genomes, but reports have indicated areas that would benefit from updates, including assembly coverage and length [4,5].SOAPdenovo2, as with SOAPdenovo, is made up of six modules that handle read error correction, de Bruijn graph (DBG) construction, contig assembly, paired-end (PE) reads mapping, scaffold construction, and gap closure. The major improvements we have made for in SOAPdenovo2 are: 1) enhancing the error correction algorithm, 2) providing a reduction in memory consumption in DBG constructions, 3) resolving longer repeat regions in contig assembly, 4) increasing assembly length and cover
Arabidopsis Fatty Acid Desaturase FAD2 Is Required for Salt Tolerance during Seed Germination and Early Seedling Growth
Jiantao Zhang,Hua Liu,Jian Sun,Bei Li,Qiang Zhu,Shaoliang Chen,Hongxia Zhang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0030355
Abstract: Fatty acid desaturases play important role in plant responses to abiotic stresses. However, their exact function in plant resistance to salt stress is unknown. In this work, we provide the evidence that FAD2, an endoplasmic reticulum localized ω-6 desaturase, is required for salt tolerance in Arabidopsis. Using vacuolar and plasma membrane vesicles prepared from the leaves of wild-type (Col-0) and the loss-of-function Arabidopsis mutant, fad2, which lacks the functional FAD2, we examined the fatty acid composition and Na+-dependent H+ movements of the isolated vesicles. We observed that, when compared to Col-0, the level of vacuolar and plasma membrane polyunsaturation was lower, and the Na+/H+ exchange activity was reduced in vacuolar and plasma membrane vesicles isolated from fad2 mutant. Consistent with the reduced Na+/H+ exchange activity, fad2 accumulated more Na+ in the cytoplasm of root cells, and was more sensitive to salt stress during seed germination and early seedling growth, as indicated by CoroNa-Green staining, net Na+ efflux and salt tolerance analyses. Our results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers, and thereby to maintain a low cytosolic Na+ concentration for salt tolerance during seed germination and early seedling growth in Arabidopsis.
Biodistribution of fullerene derivative C60(OH)x(O)y
Qingnuan Li,Yan Xiu,Xiaodong Zhang,Ruili Liu,Qianqian Du,Xiaoguang Sun,Shaoliang Chen,Wenxin Li
Chinese Science Bulletin , 2001, DOI: 10.1007/BF02900619
Abstract: The biological behavior of fullerene derivatives shows their considerable potential application in medicine field. To understand the biodistribution of C60 derivatives as drugs or drug carriers, a simple water-soluble derivative C60(OH)x(O)y was labeled with99mTc. A γ-counter and a single photo emission computed tomography (SPECT) were used to assess the biodistribution and metabolism of the labeled compound in mice and rabbits, respectively. The results showed that the compound could be absorbed rapidly by tissues, especially by coronal bone, breastbone, backbone, extremity honeycomb, liver and spleen. The clearance was slow from all tissues except for brain. The compound might be excreted through urine and enteron. The biodistribution shows some difference from that of Yamago et al. In this note, we discuss the possible reason leading to the difference. Further study is needed to find out to what extent the C60 itself determines the biodistribution of derivatives.
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