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Search Results: 1 - 10 of 92626 matches for " Shang-jun Yang "
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Row stochastic inverse eigenvalue problem
Shang-jun Yang,Chang-qing Xu
Journal of Inequalities and Applications , 2011,
Abstract: In this paper, we give sufficient conditions or realizability criteria for the existence of a row stochastic matrix with a given spectrum Λ = {λ1, ..., λn} = Λ1 ∪ ∪ Λm ∪ Λm+1, m > 0; where (pk is an integer greater than 1), λk1 = λk > 0, 1 = λ1 ≥ ωk > 0, k = 1, ..., m; Λm+1 = {λm+1}, ωm+1 ≡ λ1 + ..., +λn ≤ λ1, ωk ≥ λk, ω1 ≥ λk, k = 2, ..., m + 1. In the case when p1, ..., pm are all equal to 2, Λ becomes a list of 2m + 1 real numbers for any positive integer m, and our result gives sufficient conditions for a list of 2m + 1 real numbers to be realizable by a row stochastic matrix. AMS classification: 15A18.
A Note on Algorithms for Determining the Copositivity of a Given Symmetric Matrix
Yang Shang-jun,Xu Chang-qing,Li Xiao-xin
Journal of Inequalities and Applications , 2010, DOI: 10.1155/2010/498631
Abstract: In the previous paper by the first and the third authors, we present six algorithms for determining whether a given symmetric matrix is strictly copositive, copositive (but not strictly), or not copositive. The algorithms for matrices of order n≥8 are not guaranteed to produce an answer. It also shows that for 1000 symmetric random matrices of order 8, 9, and 10 with unit diagonal and with positive entries all being less than or equal to 1 and negative entries all being greater than or equal to 1, there are 8, 6, and 2 matrices remaing undetermined, respectively. In this paper we give two more algorithms for n=8,9 and our experiment shows that no such matrix of order 8 or 9 remains undetermined; and almost always no such matrix of order 10 remains undetermined. We also do some discussion based on our experimental results.
A Note on Algorithms for Determining the Copositivity of a Given Symmetric Matrix
Shang-jun Yang,Chang-qing Xu,Xiao-xin Li
Journal of Inequalities and Applications , 2010,
Abstract: In the previous paper by the first and the third authors, we present six algorithms for determining whether a given symmetric matrix is strictly copositive, copositive (but not strictly), or not copositive. The algorithms for matrices of order are not guaranteed to produce an answer. It also shows that for 1000 symmetric random matrices of order 8, 9, and 10 with unit diagonal and with positive entries all being less than or equal to 1 and negative entries all being greater than or equal to , there are 8, 6, and 2 matrices remaing undetermined, respectively. In this paper we give two more algorithms for and our experiment shows that no such matrix of order 8 or 9 remains undetermined; and almost always no such matrix of order 10 remains undetermined. We also do some discussion based on our experimental results.
Towards -Induced Manganese-Containing Superoxide Dismutase Inactivation and Conformational Changes: An Integrating Study with Docking Simulations
Jiang-Liu Yang,Shang-Jun Yin,Yue-Xiu Si,Zhi-Rong Lü,Xiangrong Shao,Daeui Park,Hae Young Chung,Hai-Meng Zhou,Guo-Ying Qian,Zi-Ping Zhang
Enzyme Research , 2011, DOI: 10.4061/2011/307464
Abstract: Superoxide dismutase (SOD, EC 1.15.1.1) plays an important antioxidant defense role in skins exposed to oxygen. We studied the inhibitory effects of Al3+ on the activity and conformation of manganese-containing SOD (Mn-SOD). Mn-SOD was significantly inactivated by Al3+ in a dose-dependent manner. The kinetic studies showed that Al3+ inactivated Mn-SOD follows the first-order reaction. Al3+ increased the degree of secondary structure of Mn-SOD and also disrupted the tertiary structure of Mn-SOD, which directly resulted in enzyme inactivation. We further simulated the docking between Mn-SOD and Al3+ (binding energy for Dock 6.3: ?14.07?kcal/mol) and suggested that ASP152 and GLU157 residues were predicted to interact with Al3+, which are not located in the Mn-contained active site. Our results provide insight into the inactivation of Mn-SOD during unfolding in the presence of Al3+ and allow us to describe a ligand binding via inhibition kinetics combined with the computational prediction. 1. Introduction Superoxide dismutases (SOD, EC 1.15.1.1) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide [1–3]. They play an important antioxidant defense role in skins exposed to oxygen. In this regard, for the treatment of systemic inflammatory diseases including skin ulcer lesions, the topical application of free Mn-SOD or Cu, Zn-SOD extracted from bovine, bacterial, and other species was dramatically effective in skin lesions [4]. It has been reported that significant increase in the levels of SOD occurs in vitiligo patients due to the increased oxidative stress [5]. The involvement of oxidative stress in chronic idiopathic urticaria associated with SOD was also reported [6]: the activity of SOD was markedly increased in lesional skin as compared with skin of healthy subjects, indicating that oxidative stress is crucially involved in chronic idiopathic urticaria and suggesting that oxidative stress is secondary to the development of inflammation. The earlier reports [7, 8] suggested that the activity of activator protein-1, which is associated with tumor promotion, was reduced in Mn-SOD transgenic mice overexpressing Mn-SOD in the skin, suggesting that Mn-SOD reduced tumor incidence by suppressing activator protein-1 activation. The mechanism of Mn-SOD catalysis is very important, and the mechanism therefore needs to be investigated from different sources using various kinetic methods. The information regarding the tertiary structure and the structural integrity of the active site of Mn-SOD is little known and in
Research on the Polymorphism of Heart Fatty Acid-binding Protein Gene in Shanxi Pig Breeds and Their Crossbred Populations Using PCR-RFLP
山西猪种及其杂种群体H-FABP基因的PCR-RFLP研究

YANG Wen-Ping,CAO Guo-Qing,XUE Shang-Jun,LIU Jian-Hua,LI Bu-Gao,ZHOU Zhong-Xiao,
杨文平
,曹果清,石建忠,刘建华,李步高,周忠孝

遗传 , 2005,
Abstract: The genetic variation in 5' - upstream (Hinf I -RFLP)and the second intron (Hinf I *-RFLP, Hae III-RFLP)of heart fatty acid-binding protein(H-FABP)gene were detected with PCR-RFLP in 286 pigs including Mashen, Shanxi white pig and their crossbred populations. The results showed as follows: (1)Mashen presented only DD genotype while other populations varied,and Mashen crossbred populations had only 2 genotypes(DD, Dd) at the Hae III-RFLP site; (2)At the Hinf I -RFLP site of the 5' -upstream region, the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. (3)At the Hinf I *-RFLP site of the second intron, only Mashen presented 2 genotypes (BB, Bb), and frequency of B allele was 0.9667. (4)At the Hae III-RFLP and Hinf I *-RFLP sites, all populations were in Hardy-weinberg equilibrium.
Inhibitory Effect of Phthalic Acid on Tyrosinase: The Mixed-Type Inhibition and Docking Simulations
Shang-Jun Yin,Yue-Xiu Si,Guo-Ying Qian
Enzyme Research , 2011, DOI: 10.4061/2011/294724
Abstract: Tyrosinase inhibition studies are needed due to the medicinal applications such as hyperpigmentation. For probing effective inhibitors of tyrosinase, a combination of computational prediction and enzymatic assay via kinetics was important. We predicted the 3D structure of tyrosinase, used a docking algorithm to simulate binding between tyrosinase and phthalic acid (PA), and studied the reversible inhibition of tyrosinase by PA. PA inhibited tyrosinase in a mixed-type manner with a =65.84±1.10 mM. Measurements of intrinsic and ANS-binding fluorescences showed that PA induced changes in the active site structure via indirect binding. Simulation was successful (binding energies for Dock6.3=?27.22 and AutoDock4.2=?0.97 kcal/mol), suggesting that PA interacts with LEU73 residue that is predicted commonly by both programs. The present study suggested that the strategy of predicting tyrosinase inhibition based on hydroxyl groups and orientation may prove useful for screening of potential tyrosinase inhibitors.
Kinetic Characterization of an Intestinal Trefoil Factor Receptor
Zhang Yong, Wang Lin, Sun Yong, Liang Guang-ping, Wu Dan, Lv Shang-jun, Wu Wei, Peng Xi
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074669
Abstract: Objective To determine whether intestinal epithelial cells have a receptor for intestinal trefoil factor and characterize receptor-ligand binding kinetics. Methods Radioligand binding assays were performed to characterize the binding kinetics between [125I]-labeled ITF and IEC-6, HT-29, Caco2 and HaCaT cells. The Kd, Bmax and other kinetic variables describing the interaction between ITF and its potential receptors were determined. Results Radioligand binding assays performed at 4°C showed that the Kd value for the association between [125I]-ITF and IEC-6, HT-29, and Caco2 cells were 1.99±0.12×10?9 M, 3.89±0.42×10?9 M, and 2.04±0.17×10?9 M, respectively. Bmax values were 1.17±0.04×1011, 3.97±0.29×1011, and 2.03±0.08×1011 sites/cell, respectively. The Ki values for the interaction between IEC-6, HT-29, and Caco2 cells and non-labeled ITF were 20.98±0.57 nM, 36.87±3.35 nM, and 21.38±0.93 nM, respectively, and the IC50 values were 25.21±0.39 nM, 40.68±0.27 nM, and 23.61±0.25 nM, respectively. Radioligand binding kinetic results showed the association rate constants (k+1) for IEC-6, HT-29, and Caco2 cells were 0.22±0.04 min?1, 0.29±0.04 min?1, and 0.26±0.05 min?1, respectively, and the dissociation rate constants (k-1) were 0.06±0.02 min?1, 0.03±0.01 min?1, and 0.04±0.01 min?1, respectively. For the HaCaT cells, the Kd was 4.86±0.28×10?8 M and Bmax was 5.81±0.15×108 sites/cell, the very low specific binding between [125I]-ITF and these cells made it impossible to calculate binding kinetic parameters. Conclusions An ITF-specific receptor appears to be present on the three types of intestinal epithelial cells (IEC-6, HT-29, and Caco-2), and there may be no ITF receptor on epidermal cells.
Optimization of Culture conditions for a methane utilizing strain and its preliminary application
甲烷利用菌培养条件的优化及其初步应用

ZHENG Jun,CHENG Hong-bing,LIU Shang-jun,ZHAO Gen-gui,
郑军
,程红兵,刘尚俊,赵艮贵

中国生物工程杂志 , 2007,
Abstract: 利用统计学实验设计对能够利用甲烷的假单胞菌菌株ME16主要培养条件进行了优化。以液体无机盐和甲烷气体作为培养基分别进行了温度、接种量、甲烷含量和培养pH对细菌生长影响的研究,并在此基础上,利用响应面法(RSM)分析优化了ME16菌株的主要培养条件,得到最佳培养条件为:温度29.4℃,接种量1.8%,甲烷含量25%。采用优化培养条件进行培养,细菌生物量增大0.8倍,达到稳定期的培养时间缩短了50h。该菌株初步应用于甲烷气体的脱除,脱除率达65.7%,表明该菌株能良好地脱除空气中的甲烷。
Study on inducement condition of tetraploid in Macrobrachium nipponense by Cytochalasin B
细胞松弛素B对日本沼虾四倍体的适宜诱导条件的研究

QIAN Guo-Ying,ZHU Qiu-Hua,WANG Cai-Sheng,YIN Shang-Jun,
钱国英
,朱秋华,汪财生,尹尚军

水生生物学报 , 2006,
Abstract: 本文研究了用细胞松弛素B(Cytochalasin B,CB)诱导日本沼虾(Macrobrachium nipponense)多倍体的处理条件和方法。试验水温分别为18—19℃、22—23℃,CB浓度为1.0—2.0mg/L,处理的起始时间在受精后5—9h,处理的持续时间为10—20min。结果表明,在水温为18—19℃时,四倍体卵子的最适诱导条件为:受精后7—8h,CB溶液的浓度1.5mg/L,处理20min,四倍体诱导率最高可达56.1%;在水温为22—23℃时,最适诱导条件为:在受精后6 h左右,CB溶液浓度为1.5mg/L,处理15—20min,四倍体诱导率最高可达54.3%。处理的起始时间和CB溶液的浓度对胚胎的四倍体率有显著影响(P<0.05);胚胎的成活率随CB浓度的提高与处理的持续时间的延长而下降。考虑到胚胎成活率因素,建议选择CB浓度为1.5mg/L,处理持续时间15—20min。
Applications of X-ray Fluorescence Spectrometry in Elemental Analysis of Crystals
X射线荧光光谱在晶体材料组成分析中的应用

ZHUO Shang-Jun,TAG Guang-Yi,JI Ang,SHENG Cheng,SHEN Ru-Xiang,
卓尚军
,陶光仪,吉昂,盛成,申如香

无机材料学报 , 2003,
Abstract: The applications of X-ray fluorescence spectrometry in elemental analysis of crystals were reviewed. Although it is an effective and efficient approach for elemental analysis, X-ray fluorescence analyses require different sample preparation methods depending on different sample types and require calibration standards to correct matrix effects. Abnormal reflection may lead to complicated background and confused qualitative interpretation or/and quantitative measuring conditions if the measured specimen is a single crystal. In this case, the fusion method is preferable. Attention must be paid to the uncertainty of the measurement when the results are employed to a specific purpose.
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