oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Search Results: 1 - 8 of 8 matches for " Segenet Kelemu "
All listed articles are free for downloading (OA Articles)
Page 1 /8
Display every page Item
几丁质酶基因在大肠杆菌中的表达及其酶对柱花草胶孢炭疽病原菌的抑菌效果
蒋昌顺,邹冬梅,Segenet,Kelemu
草地学报 , 2000, DOI: 10.11733/j.issn.1007-0435.2000.02.005
Abstract: 构建了几丁质酶基因植物表达载体,该基因在大肠杆菌中能表达35kD的蛋白质。几丁质酶粗提物在体外对柱花草胶孢炭疽病原菌有极显著的抑菌效果。
旗草内生真菌与旗草抗病性研究
黄东益,黄小龙,SEGENET Kelemu
草业学报 , 2009,
Abstract: 旗草内生真菌交织顶孢霉的7个不同分离物对旗草主要病源真菌德氏霉和立枯丝核菌在PDA培养基上的体外对峙试验表明,大多数内生真菌分离物对这2个病原菌都有不同程度的抑制作用,不同分离物对同一病原菌的抑制作用不同,同一分离物对不同病原菌的抑制作用也不同,EB6780.501和EH32a对德氏霉和立枯丝核菌的抑制作用最强。旗草植株抗病试验表明,内生真菌感染的植株对叶枯病具有明显的抗性,但这种抗性随着病原菌立枯丝核菌入侵时间的延长而减弱。在体外抑菌试验中,对病原菌生长抑制能力强的内生真菌分离物,在旗草体内也表现出较强的抗病性,说明抗病内生真菌的体外抑菌筛选是有效的。
Molecular identification and characterization of Colletotrichum sp. isolates from Tahiti lime, tamarillo, and mango
Sanabria Adriana,Mahuku George,Kelemu Segenet,Cadavid Marcela
Agronomía Colombiana , 2010,
Abstract: Anthracnose is a very limiting disease affecting production, as well as postharvest quality of numerous fruit crops in Colombia. The current management practices for this disease are partially effective due to limited information about the etiology, the inoculum sources, population structure and variation of the pathogen. A total of 293 Colletotrichum isolates were obtained from symptomatic tissues collected from Tahiti lime, tamarillo and mango orchards. To determine the Colletotrichum species causing the symptoms, amplification, and PCR product analysis for intergenic regions of the ribosomal DNA were conducted. Genetic diversity of the fungal population was assessed with Random Amplified Microsatellites (RAMS). Results of this study indicated that anthracnose in Tahiti lime and tamarillo are caused by Colletotrichun acutatum whereas symptoms on mango were induced by the species Colletotrichum gloeosporioides, which was also fund in few citrus samples. RAMS data analysis indicated the existence of two distinct species groups, with a low similarity index (35%). RAM profiles also showed a clear host differentiation of isolates. The C. acutatum population originated from tamarillo exhibited a narrow and homogeneous genetic base, while the C. acutatum
Molecular identification and characterization of Colletotrichum spp isolates from tahiti lime, tamarillo, and mango
Sanabria,Adriana; Mahuku,George; Kelemu,Segenet; Cadavid,Marcela; García,Celsa; Hío,Juan C; Martínez,érika; Osorio,Jairo A.;
Agronomía Colombiana , 2010,
Abstract: anthracnose is a very limiting disease affecting production, as well as postharvest quality of numerous fruit crops in colombia. the current management practices for this disease are partially effective due to limited information about the etiology, the inoculum sources, population structure and variation of the pathogen. a total of 293 colletotrichum isolates were obtained from symptomatic tissues collected from tahiti lime, tamarillo and mango orchards. to determine the colletotrichum species causing the symptoms, amplification, and pcr product analysis for intergenic regions of the ribosomal dna were conducted. genetic diversity of the fungal population was assessed with random amplified microsatellites (rams). results of this study indicated that anthracnose in tahiti lime and tamarillo are caused by colletotrichun acutatum whereas symptoms on mango were induced by the species colletotrichum gloeosporioides, which was also fund in few citrus samples. rams data analysis indicated the existence of two distinct species groups, with a low similarity index (35%). ram profiles also showed a clear host differentiation of isolates. the c. acutatum population originated from tamarillo exhibited a narrow and homogeneous genetic base, while the c. acutatum population from tahiti lime was more heterogeneous and genetically complex, as determined by the analysis of molecular variance (amova) and of ni-li coefficient. the c. gloeosporioides population originated from mango and tahiti lime was heterogeneous and highly diverse, with clear host differentiation according to ram profiles. collectively, the results from this study provide new insight into the general characteristics of colletotrichum populations on various hosts; this type of knowledge will prove useful in designing more effective management practices.
Harmonizing the agricultural biotechnology debate for the benefit of African farmers
Segenet Kelemu, George Mahuku, Martin Fregene, Douglas Pachino, Nancy Johnson, Lee Calvert, Idupulapati Rao, Robin Buruchara, Tilahun Amede, Paul Kimani, Roger Kirkby, Susan Kaaria, Kwasi Ampofo
African Journal of Biotechnology , 2003,
Abstract: The intense debate over agricultural biotechnology is at once fascinating, confusing and disappointing. It is complicated by issues of ethical, moral, socio-economic, political, philosophical and scientific import. Its vocal champions exaggerate their claims of biotechnology as saviour of the poor and hungry, while, equally loudly, its opponents declare it as the doomsday devil of agriculture. Sandwiched between these two camps is the rest of the public, either absorbed or indifferent. Biotechnology issues specific to the African public must include crop and animal productivity, food security, alleviation of poverty and gender equity, and must exclude political considerations. Food and its availability are basic human rights issues—for people without food, everything else is insignificant. Although we should discuss and challenge new technologies and their products, bringing the agricultural biotechnology debate into food aid for Africa where millions are faced with life-or-death situations is irresponsible. Agricultural biotechnology promises the impoverished African a means to improve food security and reduce pressures on the environment, provided the perceived risks associated with the technology are addressed. This paper attempts to harmonize the debate, and to examine the potential benefits and risks that agricultural biotechnology brings to African farmers.
Assessment of Knowledge and Practice towards Hepatitis B among Medical and Health Science Students in Haramaya University, Ethiopia
Yonatan Moges Mesfin, Kelemu Tilahun Kibret
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0079642
Abstract: Introduction Hepatitis B (HB) is a serious infection that affects liver and caused by hepatitis B virus (HBV). HB is a serious global public health problem and the health professionals are most at risk. It is contagious and easy to be transmitted from one infected individual to another by blood to blood contact, mother to child, unprotected sexual intercourse, sharing of eating utensils and other barber shop and beauty salon equipment. The aim of this study was to assess knowledge and practices about transmissions and prevention of hepatitis B among medical and health science students on clinical attachment in Haramaya University. Methods and Findings A cross sectional study was conducted among 322 health science and medical students who are starting clinical attachment (year II, III, IV, V and IV) from February 1–15, 2013. Self-administered structured questionnaire was used to collect information. Out of 322 distributed questionnaires, 322 were returned with a response rate of 100.0%. Majority of the students (91%) were in the age group 20–24 and 232 (72%) of the respondents were male. Majorities (95.3%) of students were not fully vaccinated against Hepatitis B and 48.4% of the students were not aware about the availability of post exposure prophylaxis for HB. Mean scores for knowledge and practice were 11.52±2.37 and 2.76±1.1 respectively. Significant and positive linear correlations between knowledge-practice (r = 0.173, p = 0.002) was observed. Study department was significantly associated with mean knowledge and practice of study respondents. Conclusion This study indicates that lack of awareness about Hepatitis B, its route of transmission and modes of prevention among the medical students entering into the profession. Similarly, 95.3% the students were not fully vaccinated against Hepatitis B, which makes them vulnerable to the disease.
Association between HIV/AIDS and Multi-Drug Resistance Tuberculosis: A Systematic Review and Meta-Analysis
Yonatan Moges Mesfin, Damen Hailemariam, Sibhatu Biadglign, Kelemu Tilahun Kibret
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0082235
Abstract: Background Human immunodeficiency virus (HIV), multi-drug resistant tuberculosis (MDR) is emerging as major challenge facing tuberculosis control programs worldwide particularly in Asia and Africa. Findings from different studies on associations of HIV co-infection and drug resistance among patients with TB have been contradictory (discordant). Some institution based studies found strongly increased risks for multi-drug resistant TB (MDR TB) among patients co-infected with TB and HIV, whereas other studies found no increased risk (it remains less clear in community based studies. The aim was to conduct a systematic review and meta-analysis of the association between multi-drug resistant tuberculosis and HIV infection. Methods and findings Systematic review of the published literature of observational studies was conducted. Original studies were identified using databases of Medline/Pubmed, Google Scholar and HINARI. The descriptions of original studies were made using frequency and forest plot. Publication bias was assessed using Funnel plot graphically and Egger weighted and Begg rank regression tests statistically. Heterogeneity across studies was checked using Cochrane Q test statistic and I2. Pool risk estimates of MDR-TB and sub-grouping analysis were computed to analyze associations with HIV. Random effects of the meta-analysis of all 24 observational studies showed that HIV is associated with a marginal increased risk of multi-drug resistant tuberculosis (estimated Pooled OR 1.24; 95%, 1.04–1.43). Subgroup analyses showed that effect estimates were higher (Pooled OR 2.28; 95%, 1.52–3.04) for primary multi-drug resistance tuberculosis and moderate association between HIV/AIDS and MDR-TB among population based studies and no significant association in institution settings. Conclusions This study demonstrated that there is association between MDR-TB and HIV. Capacity for diagnosis of MDR-TB and initiating and scale up of antiretroviral treatment, and collaborations between HIV and TB control programs need to be considered and strengthened.
Determinant Factors Associated with Occurrence of Tuberculosis among Adult People Living with HIV after Antiretroviral Treatment Initiation in Addis Ababa, Ethiopia: A Case Control Study
Kelemu Tilahun Kibret, Alemayehu Worku Yalew, Belaineh Girma Belaineh, Muluken Melese Asres
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0064488
Abstract: Introduction Tuberculosis (TB) is a leading morbidity and mortality, and the first presenting sign in majority of people living with Human Immune deficiency Virus (PLWH). Determinants of active TB among HIV patients on anti retroviral treatment (ART) are not well described in resource limited settings. The aim of this study was to assess determinant factors for the occurrence of TB among people living with HIV after ART initiation in public hospitals and health centers in Addis Ababa, Ethiopia. Methods and Findings A case control study was conducted from December 2011 to February 2012 in 2 public hospitals and 13 health centers in Addis Ababa. The study population consisted of 204 cases and 409 controls. Cases were adult people living with HIV who developed TB after ART initiation and controls were adult people living with HIV who did not develop TB after ART initiation. An interviewer administered structured questionnaire was used to collect information. After adjustment for potential confounders, presence of isoniazid prophylaxis (adjusted odd ratio [AOR] 0.35, 95% confidence interval [CI] 0.125, 0.69) and cotrimoxazole prophylaxis (AOR = 0.19; 95% CI: 0.06, 0.62) had protective benefit against risk of TB. In contrary, bedridden (AOR = 9.36; 95% CI: 3.39, 25.85), having World Health Organization (WHO) clinical stage III/IV (AOR = 3.40; 95% CI: 1.69, 6.87) and hemoglobin level <10 mg/dl (AOR = 7.43; 95% CI; 3.04, 18.31) at enrollment to ART care were predictors for increased risk of tuberculosis in PLWH after ART initiation. Conclusion Increasing coverage of isoniazid preventive therapy and cotrimoxazole preventive therapy reduced risk of TB among HIV patients who started treatment. All PLWH should be screened for TB, but for patients who have advanced disease condition (WHO clinical stage III/IV, being bedridden and having hemoglobin level <10 mg/dl) intensified screening is highly recommended during treatment follow up.
Page 1 /8
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.