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Search Results: 1 - 10 of 138690 matches for " Satyavani K "
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Phylogeny of Indian Rhizophoraceae Based on the Molecular Data from Chloroplast tRNALEUUAA Intergenic Sequences
S. Gurudeeban,K. Satyavani,T. Ramanathan
Pakistan Journal of Biological Sciences , 2013,
Abstract: Molecular identification data of unexplored Indian Rhizophoraceae an eco-friendly mangroves are an imperative aspect in molecular phylogenetics. We describe the phylogenetic relationships among the Rhizophoraceae genus Rhizophora, Ceriops and Bruguiera using tRNA Leu (UAA) intron sequences as a molecular marker. The results of present study reveals congeneric relationship between R. apiculata, R. mucronata, B. gymnorhiza indicating a high degree of gene flow within them and the pairwise distribution of study plants among Rhizophoraceae family. The phylogram constructed using tRNA Leu sequence clearly clustered the species of the same genus in individual group. The stem-loop could be divided into two classes, both built up from two base pairing heptanucleotide repeats. Size variation was primarily caused by different numbers of repeats but some strains also contained additional sequences in this stem-loop. The statistical summaries of DNA sequence data can enable to identify the structural signature of the genome as well as to classify phylogenetic relationships among different species reflected in the difference of genetic diversity distributions within their DNA sequences.
Genetic Identification of Ceriops decandra (Chiru Kandal) using tRNA (Leu) Molecular Marker
S. Gurudeeban,K. Satyavani,T. Ramanathan
Asian Journal of Plant Sciences , 2012,
Abstract: Ceriops decandra is a glabrous shrub belongs to a family Rhizophoraceae distributed in a region including Southeast India, East Africa and Australia. The present study aimed to identify a tool in identifying the mangrove at the molecular level. The chloroplast trnL region was amplified from extracted total genomic DNA using the Polymerase Chain Reaction (PCR) and sequenced. Sequence of the principal agarose gel band revealed that Ceriops decandra and deposited in NCBI with the accession No. JN871232.
Bitter Apple (Citrullus colocynthis): An Overview of Chemical Composition and Biomedical Potentials
S. Gurudeeban,K. Satyavani,T. Ramanathan
Asian Journal of Plant Sciences , 2010,
Abstract: The present study reveals the medicinal uses, chemical compositions and examines recent investigations on the therapeutic activities of extracts and chemicals identified from Citrullus colocynthis. The article also presents some of the functions of the chemicals present and attempt to emphasize and create an awareness of the great potential of Citrullus colocynthis as a source of novel agrochemicals, compounds of medicinal value and biologically active compounds.
Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad
Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T
Journal of Nanobiotechnology , 2011, DOI: 10.1186/1477-3155-9-43
Abstract: The callus extract react with silver nitrate solution confirmed silver nanoparticles synthesis through the steady change of greenish colour to reddish brown and characterized by using FT-IR, AFM. Toxicity on HEp 2 cell line assessed using MTT assay, caspase -3 assay, Lactate dehydrogenase leakage assay and DNA fragmentation assay.The synthesized silver nanoparticles were generally found to be spherical in shape with size 31 nm by AFM. The molar concentration of the silver nanoparticles solution in our present study is 1100 nM/10 mL. The results exhibit that silver nanoparticles mediate a dose-dependent toxicity for the cell tested, and the silver nanoparticles at 500 nM decreased the viability of HEp 2 cells to 50% of the initial level. LDH activities found to be significantly elevated after 48 h of exposure in the medium containing silver nanoparticles when compared to the control and Caspase 3 activation suggested that silver nanoparticles caused cell death through apoptosis, which was further supported by cellular DNA fragmentation, showed that the silver nanoparticles treated HEp2 cells exhibited extensive double strand breaks, thereby yielding a ladder appearance (Lane 2), while the DNA of control HEp2 cells supplemented with 10% serum exhibited minimum breakage (Lane 1). This study revealed completely would eliminate the use of expensive drug for cancer treatment.Citrullus colocynthis (Bitter cucumber) belongs to the family of cucurbitaceae, which are abundantly grown along the arid soils of Southeast coast of Tamil Nadu. It has a large, fleshy perennial root, which sends out slender, tough, angular, scabrid vine-like stems. The therapeutic potentials viz., antimicrobial [1], anti inflammatory [2], anti diabetic [3] and anti oxidant [4] effect of Citrullus colocynthis have reported in our laboratory. For conservation of this potent medicinal plant we have micro propagated and transplanted to the coastal region of Parangipettai.Nanoparticles usually referred as
Direct Organogenesis of Seaside Heliotrope (Heliotropium crassavicum) Using Stem Explants
K. Satyavani,V. Dheepak,S. Gurudeeban,T. Ramanathan
Pakistan Journal of Biological Sciences , 2013,
Abstract: Heliotropium crassavicum L. is a sand binder salt marsh herb with enormous traditional value and widely found in South Asia America and Europe. In the direct method of regeneration from stem explants, we observed the maximum number of shoot regeneration after four weeks culture of MS elongation medium with 2.0 mg L-1 of 2, 4-D (17.27±0.51). It was clear that MS medium with 2.0 mg mL-1 2, 4-D alone suitable for shoot multiplication as well as shoot elongation then compared to other combination of auxin and cytokinin. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing 2, 4-dichlorophenoxy acetic acid (0.5-3.0 mg L-1). The maximum number of root regeneration (6.4±0.416) and root length (6.08±0.07) were observed in MS rooting medium fortified with 2.5 mg L-1 of 2, 4-D after 2 weeks of culture. 85% of in vitro raised plantlets with well-developed shoots and roots were transferred to ex vivo conditions into polythene bag containing sterile compost with ratio (v/v/v) of organic fertilizer: sand: peat (1: 2: 2; 3: 1:0 or 2: 2: 1). Sixty five percent of acclimated plants were transferred to the pots under full sun where they grew well without any detectable phenotypic variations.
Toxicity Assessment of Expired Pesticides to Green Algae Pseudokirchneriella subcapitata
G. Satyavani,G. Chandrasehar,K. Krishna Varma,A. Goparaju
ISRN Toxicology , 2012, DOI: 10.5402/2012/247072
Biochemical Indicators for Rooting in Casuarina equisetifolia Clones
K. Satyavani,Kannan C.S. Warrier,T. Ramanathan,S. Gurudeeban
Asian Journal of Plant Sciences , 2010,
Abstract: Casuarina equisetifolia is the wide spread exotic trees in peninsular Indian and well known member of the family Casuarinacea. The present study was carried out biochemical indicators of ten good rooting and poor rooting clones of Casuarina equisetifolia. The DNS and anthrone method of reducing sugar and carbohydrate was estimated respectively. The result of the analysis revealed significant difference between the good rooting and poor rooting clones with higher the content reducing sugar (6.99-12.68) in better rooting, lesser the content of carbohydrates (20.09-58.59) present in better rooting. The present investigation reveals reducing sugar and carbohydrates plays a major role in rooting of Casuarina equisetifolia.
Ion Hydration and Associated Defects in Hydrogen Bond Network of Water: Observation of Reorientationally Slow Water Molecules Beyond First Hydration Shell in Aqueous Solutions of MgCl$_2$
Upayan Baul,Satyavani Vemparala
Physics , 2014, DOI: 10.1103/PhysRevE.91.012114
Abstract: Effects of presence of ions, at moderate to high concentrations, on dynamical properties of water molecules are investigated through classical molecular dynamics simulations using two well known non-polarizable water models. Simulations reveal that the presence of magnesium chloride (MgCl$_2$) induces perturbations in the hydrogen bond network of water leading to the formation of bulk-like domains with \textquoteleft defect sites\textquoteright~on boundaries of such domains: water molecules at such defect sites have less number of hydrogen bonds than those in bulk water. Reorientational autocorrelation functions for dipole vectors of such defect water molecules are computed at different concentrations of ions and compared with system of pure water. Earlier experimental and simulation studies indicate significant differences in reorientational dynamics for water molecules in the first hydration shell of many dissolved ions. Results of this study suggest that defect water molecules, which are beyond the first hydration shells of ions, also experience significant slowing down of reorientation times as a function of concentration in the case of MgCl$_2$. However, addition of cesium chloride(CsCl) to water does not perturb the hydrogen bond network of water significantly even at higher concentrations. This difference in behavior between MgCl$_2$ and CsCl is consistent with the well-known Hofmeister series.
Atomistic simulations of a multicomponent asymmetric lipid bilayer
Anirban Polley,Satyavani Vemparala,Madan Rao
Physics , 2012,
Abstract: The cell membrane is inherently asymmetric and heterogeneous in its composition, a feature that is crucial for its function. Using atomistic molecular dynamics simulations, the physical properties of a 3-component asymmetric mixed lipid bilayer system comprising of an unsaturated POPC (palmitoyl-oleoyl-phosphatidyl-choline), a saturated SM (sphingomyelin) and cholesterol are investigated. In these simulations, the initial stages of liquid ordered, $l_o$, domain formation are observed and such domains are found to be highly enriched in cholesterol and SM. The current simulations also suggest that the cholesterol molecules may partition into these SM-dominated regions in the ratio of $3:1$ when compared to POPC-dominated regions. SM molecules exhibit a measurable tilt and long range tilt correlations are observed within the $l_o$ domain as a consequence of the asymmetry of the bilayer, with implications to local membrane deformation and budding. Tagged particle diffusion for SM and cholesterol molecules, which reflects spatial variations in the physical environment encountered by the tagged particle, is computed and compared with recent experimental results obtained from high resolution microscopy.
Aggregation of rod-like polyelectrolyte chains in the presence of monovalent counterions
Anoop Varghese,R. Rajesh,Satyavani Vemparala
Physics , 2012, DOI: 10.1063/1.4771920
Abstract: Using molecular dynamics simulations, it is demonstrated that monovalent counterions can induce aggregation of similarly charged rod-like polyelectrolyte chains. The critical value of the linear charge density for aggregation is shown to be close to the critical value for the extended-collapsed transition of a single flexible polyelectrolyte chain, and decreases with increasing valency of the counterions. The effective interaction potential between two rod-like polyelectrolyte chains is measured, and is related to the angular distribution of the condensed counterions.
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