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Search Results: 1 - 10 of 6739 matches for " Sarah Read "
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The negotiation of writer identity in engineering faculty - writing consultant collaborations
Sarah Read
Journal of Writing Research , 2011,
Abstract: Negotiating faculty-writing consultant collaborations in engineering contexts can be challenging when the writing consultant originates in the humanities. The author found that one of the sites of negotiation in the formation of working relationships is that of writer identity, and disciplinary writer identity in particular. In order to confirm her experiential knowledge, the author interviewed her faculty collaborators to further investigate their attitudes and experiences about writing. Analysis of two excerpts of these interviews makes visible "clashes" between the faculty engineers' and the writing consultant's autobiographical and disciplinary writer identities. Implications of the role of writer identity in faculty-writing consultant collaborations include considering the value of extending this negotiation explicitly to students and the question of how writing curriculum can explicitly engage students in the formation of positive disciplinary writer identities
Additive and Transcript-Specific Effects of KPAP1 and TbRND Activities on 3′ Non-Encoded Tail Characteristics and mRNA Stability in Trypanosoma brucei
Sara L. Zimmer, Sarah M. McEvoy, Sarita Menon, Laurie K. Read
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0037639
Abstract: Short, non-encoded oligo(A), oligo(U), or A/U tails can impact mRNA stability in kinetoplastid mitochondria. However, a comprehensive picture of the relative effects of these modifications in RNA stability is lacking. Furthermore, while the U-preferring exoribonuclease TbRND acts on U-tailed gRNAs, its role in decay of uridylated mRNAs has only been cursorily investigated. Here, we analyzed the roles of mRNA 3′ tail composition and TbRND in RNA decay using cells harbouring single or double knockdown of TbRND and the KPAP1 poly(A) polymerase. Analysis of mRNA abundance and tail composition reveals dramatic and transcript-specific effects of adenylation and uridylation on mitochondrial RNAs. Oligo(A) and A-rich tails can stabilize a proportion of edited and never-edited RNAs. However, non-tailed RNAs are not inherently unstable, implicating additional stability determinants and/or spatial segregation of sub-populations of a given RNA in regulation of RNA decay. Oligo(U) tails, which have been shown to contribute to decay of some never-edited RNAs, are not universally destabilizing. We also show that RNAs display very different susceptibility to uridylation in the absence of KPAP1, a factor that may contribute to regulation of decay. Finally, 3′ tail composition apparently impacts the ability of an RNA to be edited.
Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum
Martin Read, Ingrid B Müller, Sarah L Mitchell, Paul FG Sims, John E Hyde
Malaria Journal , 2010, DOI: 10.1186/1475-2875-9-351
Abstract: Polyclonal antibodies were raised to PfSHMTc and PfSHMTm, and, together with specific markers for the mitochondrion and apicoplast, were employed in quantitative confocal fluorescence microscopy of blood-stage parasites.As well as the expected cytoplasmic occupancy of PfSHMTc during all stages, localization into the mitochondrion and apicoplast occurred in a stage-specific manner. Although early trophozoites lacked visible organellar PfSHMTc, a significant percentage of parasites showed such fluorescence during the mid-to-late trophozoite and schizont stages. In the case of the mitochondrion, the majority of parasites in these stages at any given time showed no marked PfSHMTc fluorescence, suggesting that its occupancy of this organelle is of limited duration. PfSHMTm showed a distinctly more pronounced mitochondrial location through most of the erythrocytic cycle and GFP-tagging of its N-terminal region confirmed the predicted presence of a mitochondrial signal sequence. Within the apicoplast, a majority of mitotic schizonts showed a marked concentration of PfSHMTc, whose localization in this organelle was less restricted than for the mitochondrion and persisted from the late trophozoite to the post-mitotic stages. PfSHMTm showed a broadly similar distribution across the cycle, but with a distinctive punctate accumulation towards the ends of elongating apicoplasts. In very late post-mitotic schizonts, both PfSHMTc and PfSHMTm were concentrated in the central region of the parasite that becomes the residual body on erythrocyte lysis and merozoite release.Both PfSHMTc and PfSHMTm show dynamic, stage-dependent localization among the different compartments of the parasite and sequence analysis suggests they may also reversibly associate with each other, a factor that may be critical to folate cofactor function, given the apparent lack of enzymic activity of PfSHMTm.Malaria parasites are a major cause of mortality and morbidity, resulting in over a million deaths each y
Does the drug sensitivity of malaria parasites depend on their virulence?
Petra Schneider, Brian HK Chan, Sarah E Reece, Andrew F Read
Malaria Journal , 2008, DOI: 10.1186/1475-2875-7-257
Abstract: Two Plasmodium chabaudi lines, one derived from the other by serial passage, were used to establish avirulent and virulent infections in mice. After five days, infections were treated with various doses of pyrimethamine administered over 1 or 4 days. Virulence measures (weight and anaemia), parasite and gametocyte dynamics were followed until day 21.All treatment regimes reduced parasite and gametocyte densities, but infections with the virulent line always produced more parasites and more gametocytes than infections with the avirulent line. Consistent with our hypothesis, drug treatment was disproportionately effective against the less virulent parasites. Treatment did not affect the relative transmission advantage of the virulent line. Neither of the lines contained known mutations conferring classical drug resistance.Drug-sensitivity of malaria parasites can be virulence-dependent, with virulent parasites more likely to survive sub-optimal treatment. If this proves to be general for a variety of drugs and parasite species, selection imposed by sub-optimal drug treatment could result in the evolution of more aggressive malaria parasites.Patients in endemic areas often carry malaria parasites while having levels of antimalarial drugs in their blood that fail to eliminate all parasites. This can be due to the presence of residual levels of drugs when a new infection is acquired [1,2], because recommended treatment could not clear all parasites [3-6] or because of inadequate treatment (for example from low quality drugs [7,8] or poor compliance). Due to variable metabolic drug uptake [9,10], recommended doses may not be adequate for all sub-groups, such as children or pregnant women [4,11,12]. Even during supervised treatment with potent and high quality drugs, including artemisinin-based combination therapies, patients can be cured from disease symptoms but low numbers of parasites to survive and transmit [5,13,14]. In all these cases, drugs are not maintained at su
A Stochastic Finite Element Model for the Dynamics of Globular Macromolecules
Robin Oliver,Daniel J. Read,Oliver G. Harlen,Sarah A. Harris
Physics , 2012, DOI: 10.1016/j.jcp.2012.12.027
Abstract: We describe a novel coarse-grained simulation method for modelling the dynamics of globular macromolecules, such as proteins. The macromolecule is treated as a continuum that is subject to thermal fluctuations. The model includes a non-linear treatment of elasticity and viscosity with thermal noise that is solved using finite element analysis. We have validated the method by demonstrating that the model provides average kinetic and potential energies that are in agreement with the classical equipartition theorem. In addition, we have performed Fourier analysis on the simulation trajectories obtained for a series of linear beams to confirm that the correct average energies are present in the first two Fourier bending modes. We have then used the new modelling method to simulate the thermal fluctuations of a representative protein over 500ns timescales. Using reasonable parameters for the material properties, we have demonstrated that the overall deformation of the biomolecule is consistent with the results obtained for proteins in general from atomistic molecular dynamics simulations.
Contemplating Design: Listening to Children’s Preferences about Classroom Design  [PDF]
Marilyn A. Read
Creative Education (CE) , 2010, DOI: 10.4236/ce.2010.12012
Abstract: This paper focuses on children’s responses about the design of two images of interior classroom environments. Chil-dren reported that key elements were circles, spheres, and windows in the low visual stimulation environment. In the high visual stimulation environment they identified activity materials and the decor as preferred elements in the space. Results from this study can be used by designers of child development centers to guide the design of the space to reflect one that incorporates children’s preferences for design.
A Case of Recurrent Mesocolon Myxoid Liposarcoma and Review of the Literature
Amar M. Eltweri,Gianpiero Gravante,Sarah Louise Read-Jones,Sonpreet Rai,David J. Bowrey,Ian Gordon Haynes
Case Reports in Oncological Medicine , 2013, DOI: 10.1155/2013/692754
Abstract: Background. Liposarcoma is the second most common soft tissue sarcoma affecting predominantly the retroperitoneal space and extremities. Mesenteric liposarcoma is uncommon and occurs in the small bowel mesentery. In this paper we report the case of a recurrent mesocolon myxoid liposarcoma manifesting 6 years from the initial right hemicolectomy for the primary tumour. Case Report. A 41-year-old female presented with a 4-day history of signs and symptoms indicative of small bowel obstruction, subsequently confirmed on plain abdominal X-ray. In 2006 she underwent a right hemicolectomy for a myxoid liposarcoma of the mesentery. The patient was initially managed conservatively; however she showed no signs of improvement and was taken to theatre for an exploratory laparotomy and division of adhesional bands. During this procedure an incidental finding of a dark purple, smooth pelvic mass was identified with similar macroscopic appearance to that of splenic tissue. Histological examination revealed a recurrent mesocolon myxoid liposarcoma. Conclusion. Mesocolon myxoid liposarcoma is a rare soft tissue neoplastic pathology and carries a high risk of recurrence. Therefore, a symptomatic patient with a previous history of primary liposarcoma excision should be treated with a high index of suspicion and a longer period of followup should be considered. 1. Introduction Liposarcoma is a group of malignancies of mesenchymal origin that arise from adipose tissue. The incidence peaks in the fourth to sixth decades of life [1]. CT and MRI are important imaging modalities in determining tissue characteristics, the size of the tumour, and invasion into surrounding structures [2]. When feasible, the main treatment is surgical resection followed by adjuvant chemotherapy and/or radiotherapy [3]. Important prognostic factors include the histological classification and tumour site and size [4] while positive surgical margins are key predictors for local recurrence [3]. Liposarcomas are usually located in the lower limbs of adults [1, 4], rarely in the small bowel mesentery and even less frequently in the mesocolon. In this report, we present the case of a recurrent mesocolon myxoid liposarcoma manifesting six years from the initial right hemicolectomy and review the literature regarding mesenteric liposarcomas. 2. Case Report A 41-year-old female presented to the emergency department with a four-day history of signs and symptoms indicative of abdominal obstruction. Her past medical history included hypothyroidism due to autoimmune thyroiditis, managed with levothyroxine. In
Purification of Laboratory Chemicals, Fourth Edition. By W. L. F. Armarego
R. Read
Molecules , 1997, DOI: 10.3390/21000152
Abstract: No abstract available
Use of a bougie during percutaneous tracheostomy
M Read
Critical Care , 2004, DOI: 10.1186/cc2469
Abstract: A bougie was used during 46. This technique does not use bronchoscopic control. A bougie is passed through the tracheal tube (TT) into the trachea. The TT is withdrawn until the cuff is above the vocal cords. With the cuff fully inflated, the TT is advanced (using the bougie as a guide) until the cuff impacts on the vocal cords. A gas-seal is maintained by gentle pressure on the TT keeping the cuff pressing on the vocal cords. During percutaneous tracheostomy the bougie remains in the trachea. When ventilation through the tracheostomy tube (cuff inflated) is confirmed, the TT and bougie are withdrawn. Throughout the procedure, if ventilation difficulties occur, the TT can be easily re-inserted using the bougie as a guide.Three different bougies were used: types (number used) were Eschmann (29), size 10 Portex disposable (four) and size 12 Portex disposable (13). Three patients were trauma cases: a neutral cervical position was maintained. In 33 cases the Blue Rhino dilator and in 12 cases the Ultra-Perc (White Rhino) dilator was used. One case was a serial dilator (see later).The bougie for airway control for percutaneous tracheostomy was associated with zero hypoxic episodes in 46 cases. Minor bougie damage in two cases caused no problems. Other complications seen were either minor or unlikely to be due to the bougie.
The Origin of the Constant Carbohydrate Diet
CharlesHerbert Read
International Journal of Pediatric Endocrinology , 2008, DOI: 10.1155/2009/469623
Abstract: Within an hour of reporting to the Winnipeg Children's Hospital as the newly appointed Assistant Professor of Pediatrics at the University of Manitoba College of Medicine in September, 1951, I was asked to assume responsibility for the diabetes service. I was totally surprised. Obviously they had not understood my postgraduate activities.After graduating from McGill Medical School, I was in the Royal Canadian Navy for three years and returned to a pediatric residency at the Montreal Children's Hospital (MCH) in 1946. During a rotation to the Royal Victoria Montreal Maternity Hospital, I became intrigued by the clinical problems that a newborn infant of a diabetic mother had. I reviewed the world's literature and found they were not even described. This led me to spending the next four years as a Fellow, two at McGill (Montreal Children's Hospital), and two at Harvard (Massachusetts General Hospital), virtually all in laboratory-based research [1, 2]. In this interval, not only was I never involved in the treatment of anyone who had diabetes, but also I did not even see a child who had it. However, I did learn the pathophysiology of diabetes and thoroughly understood Butler's method [3] for treating diabetic ketoacidosis.I quickly thought the offer over and decided that by working with the children and their parents I probably could learn the day to day problems that face children with insulin-dependent diabetes and how to cope with them. If I needed help, I certainly would ask for it, but in the past four years, I had a lot experience solving problems. So without revealing my deficiencies or my considerable anxiety, I accepted the responsibility.In the clinic, the patients and their mothers told me their major problems were with the American Dietetic, American Diabetic (ADA) diet. "Nothing seemed to work." I listened carefully to their descriptions of trying to make the triple exchanges. Even weighing portions was unsuccessful.This prestigious ADA exchange diet was
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