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Search Results: 1 - 10 of 150130 matches for " Sarah B. Geisler "
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Ordered Assembly of the Adhesive and Electrochemical Connections within Newly Formed Intercalated Disks in Primary Cultures of Adult Rat Cardiomyocytes
Sarah B. Geisler,Kathleen J. Green,Lori L. Isom,Sasha Meshinchi,Jeffrey R. Martens,Mario Delmar,Mark W. Russell
Journal of Biomedicine and Biotechnology , 2010, DOI: 10.1155/2010/624719
Abstract: The intercalated disk (ID) is a complex structure that electromechanically couples adjoining cardiac myocytes into a functional syncitium. The integrity of the disk is essential for normal cardiac function, but how the diverse elements are assembled into a fully integrated structure is not well understood. In this study, we examined the assembly of new IDs in primary cultures of adult rat cardiac myocytes. From 2 to 5 days after dissociation, the cells flatten and spread, establishing new cell-cell contacts in a manner that recapitulates the in vivo processes that occur during heart development and myocardial remodeling. As cells make contact with their neighbors, transmembrane adhesion proteins localize along the line of apposition, concentrating at the sites of membrane attachment of the terminal sarcomeres. Cx43 gap junctions and ankyrin-G, an essential cytoskeletal component of voltage gated sodium channel complexes, were secondarily recruited to membrane domains involved in cell-cell contacts. The consistent order of the assembly process suggests that there are specific scaffolding requirements for integration of the mechanical and electrochemical elements of the disk. Defining the relationships that are the foundation of disk assembly has important implications for understanding the mechanical dysfunction and cardiac arrhythmias that accompany alterations of ID architecture.
Evaluation of Soil Organic Carbon and Soil Moisture Content from Agricultural Fields in Mississippi  [PDF]
Prem B. Parajuli, Sarah Duffy
Open Journal of Soil Science (OJSS) , 2013, DOI: 10.4236/ojss.2013.32009
Abstract:

Independent observation of the effects of agricultural management practices on soil organic carbon (SOC) with soil moisture content (SMC) is essential to quantify their potential relationships for sustainable ecosystems. Soil water retention studies and soil carbon stocks have been mapped in some areas worldwide. However, few studies have been conducted in the southeastern US, particularly in Mississippi. The objectives of this research study were to collect soil samples from fields chosen to be representative of the watersheds they are contained within, analyze the soil samples for carbon content and soil moisture content, and evaluate the relationship between SOC and different parameters (land use, vertical distribution, temporal distribution, and soil moisture content). Field sites were chosen based on their compositional similarity shared with the watershed as a whole in the Town Creek watershed (TCW) and Upper Pearl River watershed (UPRW) in Mississippi. Monthly soil samples from different depths (6 inch, 12 inch, and 24 inch) were collected from crop, pasture, and forest field areas. Soil samples were analyzed using bench analysis, elemental analysis, and statistical analysis. This study was able to demonstrate the SOC distribution in the soil layers across all three land uses studied. It was also shown that there does seem to be an interactive effect of parameters such as land use type, vertical distribution, and time on carbon accretion within the soil. Results of this study also determined that the near surface (6-in) layer was found to contain significantly more carbon than either the 12 inch or 24 inch layers (p < 0.01) across all field types. There was found to be a high degree of variability within the soil moisture data and correlation between SOC and SMC. It was found that carbon amount is not influenced by SMC but SMC could be influenced by SOC.

The Outer Halo Cluster System of NGC 1399
B. Dirsch,D. Geisler,T. Richtler,J. C. Forte
Physics , 2001,
Abstract: We investigate the outer halo globular cluster population of NGC 1399. This study uses wide-field imaging of this cluster system, which covers the largest area studied with CCD photometry until now. The cluster system of NGC 1399 is found to extend further than 100 kpc from the galaxy. A population of metal-rich, as well as metal-poor clusters has been identified at these large radii. At radii smaller than 55 kpc the specific frequency of the red cluster system remains constant, while that of the blue clusters increases proportional to $r^{0.8\pm0.2}$. For larger radii, the uncertainty of the galaxy light profile does not permit any reliable statement.
Dust and molecular shells in asymptotic giant branch stars - Mid-infrared interferometric observations of R Aql, R Aqr, R Hya, W Hya and V Hya
R. Zhao-Geisler,A. Quirrenbach,R. Koehler,B. Lopez
Physics , 2012, DOI: 10.1051/0004-6361/201118150
Abstract: Mid-IR (8 - 13 micron) interferometric data of four oxygen-rich AGB stars (R Aql, R Aqr, R Hya, and W Hya) and one carbon-rich AGB star (V Hya) were obtained with MIDI/VLTI between April 2007 and September 2009. The spectrally dispersed visibility data are analyzed by fitting a circular fully limb-darkened disk (FDD). Results. The FDD diameter as function of wavelength is similar for all oxygen-rich stars. The apparent size is almost constant between 8 and 10 micron and gradually increases at wavelengths longer than 10 micron. The apparent FDD diameter in the carbon-rich star V Hya essentially decreases from 8 to 12 micron. The FDD diameters are about 2.2 times larger than the photospheric diameters estimated from K-band observations found in the literature. The silicate dust shells of R Aql, R Hya and W Hya are located fairly far away from the star, while the silicate dust shell of R Aqr and the amorphous carbon (AMC) and SiC dust shell of V Hya are found to be closer to the star at around 8 photospheric radii. Phase-to-phase variations of the diameters of the oxygen-rich stars could be measured and are on the order of 15% but with large uncertainties. From a comparison of the diameter trend with the trends in RR Sco and S Ori it can be concluded that in oxygen-rich stars the overall larger diameter originates from a warm molecular layer of H2O, and the gradual increase longward of 10 micron can be most likely attributed to the contribution of a close Al2O3 dust shell. The chromatic trend of the Gaussian FWHM in V Hya can be explained with the presence of AMC and SiC dust. The observations suggest that the formation of amorphous Al2O3 in oxygen- rich stars occurs mainly around or after visual minimum. However, no firm conclusions can be drawn concerning the mass-loss mechanism.
Quantifying Hydrologic and Water Quality Responses to Bioenergy Crops in Town Creek Watershed in Mississippi  [PDF]
Prem B. Parajuli, Sarah E. Duffy
Journal of Sustainable Bioenergy Systems (JSBS) , 2013, DOI: 10.4236/jsbs.2013.33028
Abstract:

Bioenergy crops are considered as a feedstock source, which can be grown in marginal soils. However, these crops may have different levels of crop yield potential and environmental benefits. The objectives of this study were to model and compare the effects of four bioenergy crops (corn—Zea mays, soybeanGlycine max (L.) Merr., miscanthus—Miscanthus-giganteus, and switchgrass—Panicum virgatum) in the Town Creek watershed (TCW) in northeast Mississippi using the Soil and Water Assessment Tool (SWAT) model. The calibrated SWAT model for TCW was used to quantify impacts to streamflow, crop yield, and sediment yield. The SWAT model reasonably (3·s-1) from the TCW when compared with the USGS observed stream flow (29.34 m3·s-1. In addition, model reasonably predicted (±6%) average annual corn yield (4.66 Mg·ha-1

Harmonizing health information systems with information systems in other social and economic sectors
Macfarlane,Sarah B.;
Bulletin of the World Health Organization , 2005, DOI: 10.1590/S0042-96862005000800012
Abstract: efforts to strengthen health information systems in low- and middle-income countries should include forging links with systems in other social and economic sectors. governments are seeking comprehensive socioeconomic data on the basis of which to implement strategies for poverty reduction and to monitor achievement of the millennium development goals. the health sector is looking to take action on the social factors that determine health outcomes. but there are duplications and inconsistencies between sectors in the collection, reporting, storage and analysis of socioeconomic data. national offices of statistics give higher priority to collection and analysis of economic than to social statistics. the report of the commission for africa has estimated that an additional us$ 60 million a year is needed to improve systems to collect and analyse statistics in africa. some donors recognize that such systems have been weakened by numerous international demands for indicators, and have pledged support for national initiatives to strengthen statistical systems, as well as sectoral information systems such as those in health and education. many governments are working to coordinate information systems to monitor and evaluate poverty reduction strategies. there is therefore an opportunity for the health sector to collaborate with other sectors to lever international resources to rationalize definition and measurement of indicators common to several sectors; streamline the content, frequency and timing of household surveys; and harmonize national and subnational databases that store socioeconomic data. without long-term commitment to improve training and build career structures for statisticians and information technicians working in the health and other sectors, improvements in information and statistical systems cannot be sustained.
Rhetorik im Dienst nationaler Mythenbildung. Zu Vertextungsstrategien und Funktionen des Pyren enmotivs in Jacint Verdaguers Canigó [Rhetoric in the service of the formation of myths. Strategies on textualisation and functions of the Pyrenees in Jacint Verdaguer’s Canigó]
B?sch, Sarah
Zeitschrift für Katalanistik , 2004,
Abstract: The research literature on one of the most famous literary texts of the Catalan Renaixen a, the verse epic Canigó, repeatedly alludes to the symbolic and identity establishing function of the Pyrenees in the context of the development of the Catalan national consciousness in the 19th century. Unlike the interpretations of the Pyrenees motive, that are based on both the dramatic art of the plot and the context when the work was created, the contribution concentrates on analysing the linguistic-stylistic strategies of textualisation of this motive-complex. Taking into account the semantic implications of the Pyrenees-metaphoric in other works by this author, his aim is to develop superordinated categories for the rhetoric varieties of the motive and to highlight their text inherent functions.
Quantising general relativity using QED theory, an overview and extension
Sarah B. M. Bell
Physics , 2004,
Abstract: We summarise and discuss some of our previous results, which show that Bohr's theory of the one-electron atom may be derived from the theory underpinning Quantum ElectroDynamics (QED) or vice versa, and that General Relativity may also be derived from QED theory in the classical limit, if we use Newtonian mechanics in the right frame and self-similar tesseral hierarchies. We circumvent Newton's arguments against Descartes' vortex theory to show that the inverse square law for a force combined with the equation of circular motion and Bohr's quantisation of angular momentum may be derived from the vortex theory and Special Relativity. We remark on the electro-weak interaction, the number of dimensions needed, and their connection with tesseral hierarchies.
Revisitando o conceito de cidadania: notas para uma educa??o politécnica
Geisler, Adriana;
Trabalho, Educa??o e Saúde , 2006, DOI: 10.1590/S1981-77462006000200008
Abstract: in opposition to a community and school project which have been denying the effective citizenship to a great contingent of youth and brazilian workers, the polytechnic conception emerges as a part of an emancipating project which is far from the liberal ideology and from its reflexes on the education and work fields. retaking the issue of the citizenship meaning nowadays, this text aims to identify to which citizenship concept the polytechnic conception belongs, considering the educational practices field and its connection to the current aspect of the world of work.
Expression of a prokaryotic P-type ATPase in E. coli Plasma Membranes and Purification by Ni2+-affinity chromatography
Geisler Markus
Biological Procedures Online , 1998, DOI: 10.1251/bpo9
Abstract: In order to characterize the P-type ATPase from Synechocystis 6803 [Geisler (1993) et al. J. Mol. Biol. 234, 1284] and to facilitate its purification, we expressed an N-terminal 6xHis-tagged version of the ATPase in an ATPase deficient E. coli strain. The expressed ATPase was immunodetected as a dominant band of about 97 kDa localized to the E. coli plasma membranes representing about 20-25% of the membrane protein. The purification of the Synecho-cystis 6xHis-ATPase by single-step Ni-affinity chromatography under native and denaturating conditions is described. ATPase activity and the formation of phosphointermediates verify the full function of the enzyme: the ATPase is inhibited by vanadate (IC50= 119 &mgr;M) and the formation of phosphorylated enzyme intermediates shown by acidic PAGE depends on calcium, indicating that the Synechocystis P-ATPase functions as a calcium pump.
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