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Determination of Antibiotic Sensitivity Pattern of Helicobacter pylori Isolates from South India Population  [PDF]
Khawaja Shakeel Ahmed, Anghesom Ambesajir Ghebremedhin, Aleem Ahmed Khan, Santosh K. Tiwari, J. D. Ahi, Irshad Ahmed
Advances in Microbiology (AiM) , 2012, DOI: 10.4236/aim.2012.23031
Abstract: Introduction: The importance of H. pylori as an etiological agent in gastroduodenal disease had suggested antibiotic treatment as a main target for the elimination of infection. The successful eradication of H. pylori infection was shown to resolve the gastritis, dramatically accelerate ulcer healing, reduce ulcer recurrence and the prophylactic effect on the recurrence of ulcer bleeding. Materials and Methods: In the present study a total six antimicrobial agents such as Ciprofloxacin, Metronidazole, Norfloxacin, Tetracyclin, Amoxycillin and Clarithromycin were used against 100 clinical isolates from patients with peptic ulcer, and non-ulcer dyspepsia. H. pylori was isolated from the gastric biopsies obtained from the clinical subjects. Results: Results of our study showed 100% resistance to Metronidazole (MIC > 256 μg/ml). 97% of the isolates were observed sensitive to Ciprofloxacin (MIC 0.38 μg), whereas Tetracyclin showed 96% sensitivity (MIC 0.25 μg - 0.125 μg). Resistance to amoxycillin, Norfloxacin, and Clarithromycin were 80%, 38% and 76% respectively (MIC > 256 μg/ml). Conclusion: It is observed that 96% - 97% of the clinical isolates were sensitive to Tetracycline and ciprofloxacin, where all isolates were found to be resistant to Metronidazole. Resistance to Amoxycillin and Clarithromycin were 80% and 76% respectively. Studies attributed the high level of resistance to the frequent use of the drugs to treat various other infections and ineffective drug control policy.
Primary sclerosing cholangitis and Biliary cirrhosis associated with Ulcerative colitis
Avinash Bardia,Santosh K. Tiwari,Sivaram G,Farha Anjum
Journal of Medical and Allied Sciences , 2011,
Abstract: Primary sclerosing cholangitis and Ulcerative colitis are caused by progressive inflammation of the bile duct and large intestine respectively. The existence of any plausible association between Primary sclerosing cholangitis and Ulcerative colitis remains highly elusive. Little is known about the incidence and prevalence of primary sclerosing cholangitis with concomitant Ulcerative colitis in the Indian subcontinent. We report a case of Primary sclerosing cholangitis with long standing Ulcerative colitis which later also developed Primary biliary cirrhosis.
Helicobacter pylori: “A benign fellow traveler or an unwanted inhabitant”
Santosh K. Tiwari,Aleem A. Khan,Pratibha Nallari
Journal of Medical and Allied Sciences , 2011,
Abstract: The recent decades have witnessed an alarming increase in the Helicobacter pylori associated diseases worldwide. In spite of this, deficiencies in our knowledge still exist about its exact epidemiology, the optimum method of its diagnosis and indeed about the precise role it plays in gastric carcinogenesis. In the present article, we review the available literature in an attempt to assign a definite role to this unique gastric pathogen. The acquisition of the cag-PAI has undoubtedly altered the understanding of host-microbe interactions, and growing appreciation of other potential determinants viz: vacA, iceA, babA, hrgA etc., may enable us understand the role of this organism and its gradual transition from a commensal to a pathogen.
Association of Helicobacter pylori restriction endonuclease-replacing gene, hrgA with overt gastrointestinal diseases
G, Manoj;Tiwari, Santosh K.;Sharma, Vishwas;Habeeb, Mohammed Aejaz;Khan, Aleem A.;Cm, Habibullah;
Arquivos de Gastroenterologia , 2008, DOI: 10.1590/S0004-28032008000300011
Abstract: background and aim: helicobacter pylori has been proven to be responsible for causing various gastrointestinal disorders including gastric adenocarcinoma. several genes of pathogen (the genes of the cag-pai, vaca, icea, and baba) either in combination or independently have been reported to significantly increase the risk of ulceration/gastric carcinoma, with the caga gene having the strongest predictive value. pursuit to identify new genes which could serve as a marker of overt disease progression, lead to the discovery of hrga gene. methods: fifty-six indigenous strains of h. pylori from subjects with various gastric disorder were screened to assess the status of hrga gene along with the caga gene using simple polymerase chain reaction using specific oligonucleotide primers. post-amplification, amplicons were subjected for sequencing to identify any strain specific variations in sequences from the h. pylori isolated from different disease manifestations. histopathological analysis was done to ascertain any significant change in the histological scores of subjects infected with caga+/hrga+ and caga-/hrg+ strains. results: all the 56 (100%) subjects amplified with the oligonucleotide primers specific to hrga gene, whereas 81.71% subjects showed the presence of caga gene. sequencing of the amplimers showed 99% homology. histology of the caga+/hrga+ and caga-/hrg+ subjects did not show any significant difference. conclusion: hrga gene of helicobacter pylori is not a ideal surrogate marker for identifying individuals with higher risk of developing overt gastro-duodenal diseases such as neoplasia of the stomach.
Establishing Human Lacrimal Gland Cultures with Secretory Function
Shubha Tiwari, Mohammad Javed Ali, Murali M. S. Balla, Milind N. Naik, Santosh G. Honavar, Vijay Anand P. Reddy, Geeta K. Vemuganti
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0029458
Abstract: Purpose Dry eye syndrome is a multifactorial chronic disabling disease mainly caused by the functional disruptions in the lacrimal gland. The treatment involves palliation like ocular surface lubrication and rehydration. Cell therapy involving replacement of the gland is a promising alternative for providing long-term relief to patients. This study aimed to establish functionally competent lacrimal gland cultures in–vitro and explore the presence of stem cells in the native gland and the established in-vitro cultures. Methods Fresh human lacrimal gland from patients undergoing exenteration was harvested for cultures after IRB approval. The freshly isolated cells were evaluated by flow cytometry for expression of stem cell markers ABCG2, high ALDH1 levels and c-kit. Cultures were established on Matrigel, collagen and HAM and the cultured cells evaluated for the presence of stem cell markers and differentiating markers of epithelial (E-cadherin, EpCAM), mesenchymal (Vimentin, CD90) and myofibroblastic (α-SMA, S-100) origin by flow cytometry and immunocytochemistry. The conditioned media was tested for secretory proteins (scIgA, lactoferrin, lysozyme) post carbachol (100 μM) stimulation by ELISA. Results Native human lacrimal gland expressed ABCG2 (mean±SEM: 3.1±0.61%), high ALDH1 (3.8±1.26%) and c-kit (6.7±2.0%). Lacrimal gland cultures formed a monolayer, in order of preference on Matrigel, collagen and HAM within 15–20 days, containing a heterogeneous population of stem-like and differentiated cells. The epithelial cells formed ‘spherules’ with duct like connections, suggestive of ductal origin. The levels of scIgA (47.43 to 61.56 ng/ml), lysozyme (24.36 to 144.74 ng/ml) and lactoferrin (32.45 to 40.31 ng/ml) in the conditioned media were significantly higher than the negative controls (p<0.05 for all comparisons). Conclusion The study reports the novel finding of establishing functionally competent human lacrimal gland cultures in-vitro. It also provides preliminary data on the presence of stem cells and duct-like cells in the fresh and in-vitro cultured human lacrimal gland. These significant findings could pave way for cell therapy in future.
On Second-Order Duality in Nondifferentiable Continuous Programming  [PDF]
I. Husain, Santosh K. Shrivastav
American Journal of Operations Research (AJOR) , 2012, DOI: 10.4236/ajor.2012.23035
Abstract: A Mond-Weir type second-order dual continuous programming problem associated with a class of nondifferentiable continuous programming problems is formulated. Under second-order pseudo-invexity and second-order quasi-invexity various duality theorems are established for this pair of dual continuous programming problems. A pair of dual continuous programming problems with natural boundary values is constructed and the proofs of its various duality results are briefly outlined. Further, it is shown that our results can be regarded as dynamic generalizations of corresponding (static) second-order duality theorems for a class of nondifferentiable nonlinear programming problems already studied in the literature.
Fritz John Duality in the Presence of Equality and Inequality Constraints  [PDF]
Iqbal Husain, Santosh K. Shrivastav
Applied Mathematics (AM) , 2012, DOI: 10.4236/am.2012.39151
Abstract: A dual for a nonlinear programming problem in the presence of equality and inequality constraints which represent many realistic situation, is formulated which uses Fritz John optimality conditions instead of the Karush-Kuhn-Tucker optimality conditions and does not require a constraint qualification. Various duality results, namely, weak, strong, strict-converse and converse duality theorems are established under suitable generalized convexity. A generalized Fritz John type dual to the problem is also formulated and usual duality results are proved. In essence, the duality results do not require any regularity condition if the formulations of dual problems uses Fritz John optimality conditions.
Sufficient Fritz John Type Optimality Criteria and Duality for Control Problems  [PDF]
Iqbal Husain, Santosh K. Srivastav
American Journal of Industrial and Business Management (AJIBM) , 2013, DOI: 10.4236/ajibm.2013.32029
Abstract:

Sufficient Fritz John optimality conditions are obtained for a control problem in which objective functional is pseudoconvex and constraint functions are quasiconvex or semi-strictly quasiconvex. A dual to the control problem is formulated using Fritz John type optimality criteria instead of Karush-Kuhn-Tucker optimality criteria and hence does not require a regularity condition. Various duality results amongst the control problem and its proposed dual are validated under suitable generalized convexity requirements. The relationship of our duality results to those of a nonlinear programming problem is also briefly outlined.

Non-Darcy Mixed Convection between Differentially Heated Vertical Walls Filled with a Porous Material: Application of New Modified Adomian Decomposition Method  [PDF]
A. K. Tiwari, Premlata Singh
Open Journal of Fluid Dynamics (OJFD) , 2015, DOI: 10.4236/ojfd.2015.54037
Abstract: This paper presents non-Darcy mixed convective flow of an incompressible and viscous fluid in a differentially heated vertical channel filled with a porous material in the presence of a temperature dependent source/sink. The analytical solution of fourth order non-linear ordinary differential equation for temperature field, which is formed by eliminating velocity field from system of governing equations in non-dimensional form, is obtained by using new modified Adomian decomposition method (NMADM) in terms of various parameters. In order to illustrate the interactive influences of governing parameters on the temperature and velocity fields, a numerical study of the analytical solution is performed with respect to three categories of transport processes i) when forced convection is dominated, ii) when forced and natural convection are equal and iii) when natural convection is dominated. Analysis of all categories has revealed that the temperature and velocity profiles are increasing function of modified Darcy number while decreasing function of Forchheimer number.
New Approach for the Determination of Tricyclic Antidepressant Amitriptyline Using β-Cyclodextrin-PEG System via Spectrophotomerty  [PDF]
Pankaj Soni, Santosh K. Sar, Rajmani Patel
Journal of Analytical Sciences, Methods and Instrumentation (JASMI) , 2012, DOI: 10.4236/jasmi.2012.22019
Abstract: A new and simple procedure for the spectrophotometric determination of the tricyclic antidepressant drug amitriptyline is proposed. The method is based on enhancement of sensitivity of the [AMIYTP]+ β-cyclodextrin and PEG molecules involved in formation of molecules inclusion complex, in presence of polyethylene glycol (PEG) medium. The mole ratio of [AMIYTP]+ β-cyclodextrin and PEG molecules in inclusion complex were determined by the curve fitting method. The value of molar absorptivity of {[AMIYTP: (β CD)] PEG} complex in term of the drug lies in rage of (2.20 - 2.23) × 104 L·mole–1·cm–1 at absorption maximum 242 nm. The slope, intercept and correlation co-efficient were found to be 14.21, 0.0046, and 0.998, respectively. The effect of analytical variables on the determination of the drug and composition of the ion associated complex are discussed in the paper. This method is applicable in the determina-tion of amitriptyline in the pharmaceutical preparations.
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