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Search Results: 1 - 10 of 12093 matches for " Santiesteban Torres "
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Evaluación bioquímico-inmunológica de infectados crónicos por el virus de la hepatitis B: Comportamiento de la respuesta inmune en sus contactos familiares
Santiesteban Torres,Jorge Arturo; Alerm González,Alina; González Griego,Antonio;
Revista Cubana de Investigaciones Biom??dicas , 2000,
Abstract: chronic infection by hbv is a clear and important cause of morbidity and mortality worldwide. an important problem, for the control and eradication of this disease is the existance of chronic hbv carriers and their family contacts. a biochemical and immunological study of chronic hbv reservoirs were conducted to determine the relation between the concentrations of hbv surface antigen, detected and quantified by the elisa method, and the serum viral dna concentrations; as well as the correlation between the levels of antigenemia and the transaminase values (alat). a direct linking was proved between viral dna and hbsag levels and also between antigen concentration and alat values. a significant percent of carriers with high viral charges and normal enzymatic levels was observed. the performance of immune response to hbv in those persons living with the chronic hbv-infested subjects was analyzed and the frequency of occurence of immune, sensitive and infected persons was estimated resulting in a high number of infected persons and persons living with the chronic patients without protection. qualitative and quantitative elisa were used to assess the response of antibodies to hbsag, induced by vaccines or by normal contacts of blood related and non-related persons in the family, but there was no difference among them. the hbsag concentration of the carrier was associated with levels of antibodies to hbsag; after completion of the vaccination program in blood related and non-related subjects, then the association was found to be negative in the former and the existance of shared gens linked to the control of anti-hbsag response and viral clearing were infered
Redise?o y estandarización de un método inmunoenzimático para detectar anticuerpos anti-estreptoquinasa en muestras de suero humano
López Matilla,Lien; González Griego,Antonio; Santiesteban Torres,Jorge Arturo; Alerm González,Alina;
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: a sandwich, heterogenous, qualitative, doble antigen immunoenzimatic method was standardized to detect anti-streptokinase antibodies in pure samples of human serum. different concentrations of the recovering antigen and conjugate dilutions, as well as various times and temperatures of incubation of the samples were assayed. the cut-off value of the assay and its detection limit were established and it was proved if there were some unspecificities. 0.1 mg/ml was determined as an optimal recovering concentration for a conjugate dilution of 1:100 000. the optimum conditions of incubation of the samples were 30 min at lab temperature and there were no interferences of other analytes with the technique.the optical densities between 0.282 and 0.654 were established as normal values of the technique.
Conservación de cepas vacunales de Leptospira en nitrógeno líquido
Borrero Maura,Reinier; Batista Santiesteban,Niurka; Blain Torres,Kirenia; Valdés Abreu,Yolanda;
Revista Cubana de Medicina Tropical , 2010,
Abstract: introduction: leptospira interrogans strains of cuban vaccine vax-spiral?are preserved in fletcher's semi-solid medium, which guarantees the preservation of the cell viability for a few months. objective: to evaluate the cryopreservation at liquid nitrogen of 6 different cryoprotectors as a long-term preservation method for leptospira interrogans strains used as antigens in vaccine vax-spiral? against leptospirosis. methods: viability was systematically evaluated according to the cell yield of the recently thawed strains in emjh medium. virulence stability was estimated in hamsters and antigenicity was evaluated by microscopic agglutination test using reference antisera from vaccinal serogroups. results: the use of 2.5% and 5% dimethyl sulfoxide, and 2.5% glycerol allowed quick recovery of the three strains without virulence or antigenicity depletion after 19 months of cryopreservation. conclusions: the results showed the feasibility of the cryopreservation at liquid nitrogen using suitable cryoprotectant as a preservation method for vaccinal strains of leptospira.
Estudio del transporte madre-hijo de anticuerpos antiestreptoquinasa
Lien López Matilla,Antonio González Griego,Alina Alerm González,Arturo Santiesteban Torres
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: Se estudió cómo se comporta el transporte de anticuerpos anti-estreptoquinasa entre la madre y el recién nacido y cuáles son los factores que influyen en este. Se colectaron 125 muestras sanguíneas de puérperas, por la vena antecubital y de sus respectivos hijos a partir del cordón umbilical. Se confeccionaron encuestas para la recolección de datos a partir de las historias clínicas de las madres y los recién nacidos. Se empleó un método inmunoenzimático, heterogéneo, cualitativo, tipo sandwich de doble antígeno para la detección de anticuerpos anti-estreptoquinasa. A partir de los resultados obtenidos se concluyó que existe un transporte activo de los anticuerpos anti-estreptoquinasa de la madre al feto y que este transporte se ve influenciado por la edad cronológica de la madre, la edad gestacional, el estado nutricional de la madre y el peso del recién nacido. The behavior of the transport of streptokinase antibodies from the mother to the newborn infant, as well as the factors influencing on it, were studied here. 125 blood samples were obtained from the antecubital vein of the puerperas and from the umbilical cord of their children. Surveys were done to collect data from the medical histories of the mothers and the newborn infants. An immunoenzimatic, heterogenous and qualitatitve sandwich doble antigen method was used to detect anti-streptokinase antibodies. According to the results, it was concluded that there is an active transport of anti-streptokinase antibodies from the mother to the fetus and that this transport is influenced by the chronological age of the mother, the gestational age, the mother’s nutritional status and the birth weight.
Redise o y estandarización de un método inmunoenzimático para detectar anticuerpos anti-estreptoquinasa en muestras de suero humano
Lien López Matilla,Antonio González Griego,Jorge Arturo Santiesteban Torres,Alina Alerm González
Revista Cubana de Investigaciones Biom??dicas , 2002,
Abstract: Se procedió a estandarizar un método inmunoenzimático, heterogéneo, cualitativo, tipo sandwich de doble antígeno para la detección de anticuerpos contra la estreptoquinasa en muestras puras de suero humano. Se ensayaron diferentes concentraciones del antígeno de recubrimiento y diluciones del conjugado, así como diferentes tiempos y temperaturas de incubación de las muestras. Se estableció el valor de corte del ensayo y su límite de detección y se comprobó si existían inespecificidades. Se determinó como concentración óptima de recubrimiento 0,1 μg/mL para una dilución del conjugado de 1:100 000. Las condiciones óptimas de incubación de las muestras fueron 30 min a temperatura de laboratorio y no se comprobaron interferencias de otros analitos con la técnica. Se establecieron como valores normales de la técnica las densidades ópticas que se encuentran entre 0,282 y 0,654. A sandwich, heterogenous, qualitative, doble antigen immunoenzimatic method was standardized to detect anti-streptokinase antibodies in pure samples of human serum. Different concentrations of the recovering antigen and conjugate dilutions, as well as various times and temperatures of incubation of the samples were assayed. The cut-off value of the assay and its detection limit were established and it was proved if there were some unspecificities. 0.1 mg/mL was determined as an optimal recovering concentration for a conjugate dilution of 1:100 000. The optimum conditions of incubation of the samples were 30 min at lab temperature and there were no interferences of other analytes with the technique.The optical densities between 0.282 and 0.654 were established as normal values of the technique.
Conservación de cepas vacunales de Leptospira en nitrógeno líquido Preservation for vaccinal strains of Leptospira at liquid nitrogen
Reinier Borrero Maura,Niurka Batista Santiesteban,Kirenia Blain Torres,Yolanda Valdés Abreu
Revista Cubana de Medicina Tropical , 2010,
Abstract: INTRODUCCIóN: las cepas de Leptospira interrogans que integran la vacuna cubana vax-SPIRAL son conservadas en medio semisólido de Fletcher, el cual garantiza el mantenimiento de la viabilidad de las células por pocos meses. OBJETIVO: en este estudio, se evaluó la crioconservación en nitrógeno líquido de 6 variantes de crioprotectores como método de conservación a largo plazo de las cepas de Leptospira interrogans, utilizadas como antígenos en la vacuna antileptospirósica vax-SPIRAL . MéTODOS: la viabilidad se evaluó periódicamente según el rendimiento celular alcanzado por las cepas recién descongeladas en medio EMJH. La estabilidad de la virulencia fue estimada en hámster. La antigenicidad antes y después de la crioconservación fue comparada mediante microaglutinación, frente a una batería de antisueros de referencia correspondientes a los serogrupos vacunales. RESULTADOS: solo el empleo de dimetilsulfóxido a 2,5 y 5 %, y glicerol 2,5 % como agentes crioprotectores permitió una rápida recuperación de las 3 cepas, tras 19 meses de crioconservación sin afectación de su virulencia y antigenicidad. CONCLUSIONES: los resultados obtenidos demuestran la factibilidad de la crioconservación en nitrógeno líquido con un apropiado agente crioprotector, como método de conservación de cepas vacunales de Leptospira. INTRODUCTION: Leptospira interrogans strains of Cuban vaccine vax-SPIRAL are preserved in Fletcher's semi-solid medium, which guarantees the preservation of the cell viability for a few months. OBJECTIVE: to evaluate the cryopreservation at liquid nitrogen of 6 different cryoprotectors as a long-term preservation method for Leptospira interrogans strains used as antigens in vaccine vax-SPIRAL against leptospirosis. METHODS: viability was systematically evaluated according to the cell yield of the recently thawed strains in EMJH medium. Virulence stability was estimated in hamsters and antigenicity was evaluated by microscopic agglutination test using reference antisera from vaccinal serogroups. RESULTS: the use of 2.5% and 5% dimethyl sulfoxide, and 2.5% glycerol allowed quick recovery of the three strains without virulence or antigenicity depletion after 19 months of cryopreservation. CONCLUSIONS: the results showed the feasibility of the cryopreservation at liquid nitrogen using suitable cryoprotectant as a preservation method for vaccinal strains of Leptospira.
Evaluación bioquímico-inmunológica de infectados crónicos por el virus de la hepatitis B: Comportamiento de la respuesta inmune en sus contactos familiares
Jorge Arturo Santiesteban Torres,Alina Alerm González,Antonio González Griego
Revista Cubana de Investigaciones Biom??dicas , 2000,
Abstract: La infección crónica por el virus de la hepatitis B (VHB) es una clara e importante causa de morbilidad y mortalidad en el mundo. Un importante problema para el control y la erradicación de la enfermedad es la exisniveles de anti-AgsHB, después del esquema completo de vacunación en consanguíneos y no consanguíneos, resultó ser de tipo negativa en los primeros e infirió la existencia de genes compartidos relacionados con el control de la respuesta anti-AgsHB y con el aclaramiento viral Chronic infection by HBV is a clear and important cause of morbidity and mortality worldwide. An important problem, for the control and eradication of this disease is the existance of chronic HBV carriers and their family contacts. A biochemical and immunological study of chronic HBV reservoirs were conducted to determine the relation between the concentrations of HBV surface antigen, detected and quantified by the ELISA method, and the serum viral DNA concentrations; as well as the correlation between the levels of antigenemia and the transaminase values (ALAT). A direct linking was proved between viral DNA and HbsAg levels and also between antigen concentration and ALAT values. A significant percent of carriers with high viral charges and normal enzymatic levels was observed. The performance of immune response to HBV in those persons living with the chronic HBV-infested subjects was analyzed and the frequency of occurence of immune, sensitive and infected persons was estimated resulting in a high number of infected persons and persons living with the chronic patients without protection. Qualitative and quantitative ELISA were used to assess the response of antibodies to HbsAg, induced by vaccines or by normal contacts of blood related and non-related persons in the family, but there was no difference among them. The HbsAg concentration of the carrier was associated with levels of antibodies to HbsAg; after completion of the vaccination program in blood related and non-related subjects, then the association was found to be negative in the former and the existance of shared gens linked to the control of anti-HbsAg response and viral clearing were infered
Pasteurización de soluciones de inmunoglobulinas de uso intravenoso. 1.: Uso de azúcares como estabilizantes
Cádiz Lahens,Armando; Hernández Machín,Janhna; Joó Sánchez,Lázaro; Moya Torres,Aniel; Camero Santiesteban,Amador;
Revista Cubana de Farmacia , 1999,
Abstract: pasteurization from the union of ii supernatants of 4 batches of normal plasma subjected to alcoholic fractionation was carried out using following stabilizers: sorbitol, sacarose and 30 % dextrose. the level of molecular aggregation wass determined by gel- filtration chromatography. sorbitol exhibited the best results with polymer formation ranging from 10 - 15 %; the values match those reported by other authors. according to who requirements, these polymers should be eliminated through other procedures for obtaining values under 10 %.
Complicaciones del trauma craneoencefálico severo en la unidad de cuidados intensivos pediátricos Complications of severe cranioencephalic trauma in the pediatric intensive care unit
Andrés Andrés Matos,Carlos Alberto Vázquez Torres,Leonor Verdecia Sánchez,Melba Zaldívar Santiesteban
Revista Cubana de Pediatr?-a , 2011,
Abstract: Introducción: el trauma craneoencefálico severo es responsable de serias complicaciones médicas que exacerban la lesión cerebral secundaria, y frecuentemente son la causa de muerte. Ojetivo: conocer las particularidades de las complicaciones médicas en pacientes con trauma craneoencefálico severo en la unidad de cuidados intensivos del hospital pediátrico "Octavio de la Concepción y de la Pedraja" durante los a os comprendidos entre 2007-2009. Métodos: estudio descriptivo en pacientes con trauma craneoencefálico, durante el período comprendido entre los a os 2007-2009, para analizar las complicaciones que influyeron en su evolución clínica. Resultados: la edad más representada fue la comprendida entre los 11-15 a os (56,90 %); los pacientes incluidos en el grupo de 1-5 a os resultaron más vulnerables a las complicaciones médicas. Las fracturas inestables (62,96 %), la rotura de vísceras macizas (51,85 %) y la insuficiencia respiratoria (44,44 %) fueron las manifestaciones clínicas más evidentes al momento del ingreso. En la Unidad de Cuidados Intensivos las complicaciones médicas frecuentes resultaron ser los trastornos hidroelectrolíticos (94,28 %) y la desnutrición (77,14 %). La hiperglicemia y el distrés respiratorio se relacionaron de manera significativa con la mortalidad. El 92,59 % de los fallecidos sumaron 3 puntos en la escala de Glasgow. La ventilación mecánica fue independiente de las complicaciones médicas. Conclusiones: los traumas de cráneo severos son la base para el desarrollo de varias y graves complicaciones que plantean muchos problemas en la práctica médica. Introduction: the severe cranioencephalic trauma re responsible of serious medical exacerbating the secondary cerebral lesion and frequently are the cause of death. Objective: to know the particularities of the medical complications in patients presenting with severe cranioencephalic trauma seen in the "Octavio de la Concepción y de la Pedraja" Children Hospital from 2007 to 2009. Methods: a descriptive study was conducted in patients presenting with cranioencephalic trauma from 2007 to 2009 to analyze the complication influencing in its clinical course. Results: the age with a greater predominance was that between 11-15 years (56,90 %); the patients included in the 1-5 years group were more vulnerable to medical complications. The unstable fractures (62,96 %), break of solid viscera (51,85 %) and the respiratory failure (44,44 %) were the more obvious clinical manifestations at admission. In the Intensive Care Unit the more frequent clinical complications were: hydroelectric dis
La Biblioteca Virtual de Salud de la provincia Holguín: un diagnóstico preliminar
Ca?edo Andalia,Rubén; Santiesteban Santiesteban,Mirtha; Sao Avilés,Augusto; Ríos Hidalgo,Nancy; Torres Acosta,Rafael; Díaz Ricardo,Oscar; Rojas García,Yaima; Montejo Castells,Marilis; La O Zaldívar,Juana; Pe?a Rodríguez,Karen;
ACIMED , 2003,
Abstract: aimed at the development of the medical virtual library of holguin province a preliminary diagnosis of existing conditions was carried out. the main concepts related to the virtual library are exposed, institutions with a higher scientific potential in health care areas are identified as well as the products and information services that can be moved to the web. the strategy to the introduction of the virtual library is provided as well as its extension to the different institutions of the health care system of the province. finally, some critical aspects for the success of this project are exposed.
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