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Search Results: 1 - 10 of 46310 matches for " Sant Ana Filho "
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Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purifica??o
Zeraik, Ana Eliza;Souza, Fernanda Sant'Ana de;Fatibello-Filho, Orlando;Leite, Oldair D.;
Química Nova , 2008, DOI: 10.1590/S0100-40422008000400003
Abstract: in this work we describe both a chromatographic purification procedure and a spot test for the enzyme peroxidase (pod: ec 1.11.1.7). the enzyme was obtained from crude extracts of sweet potatoes and the chromatographic enzyme purification procedure resulted in several fractions. therefore a simple, fast and economic spot test for monitoring peroxidase during the purification procedure was developed. the spot test is based on the reaction of hydrogen peroxide and guaiacol, which is catalyzed by the presence of peroxidase yielding the colored tetraguaiacol.
Phylogenetic correlograms and the evolution of body size in South American owls (Strigiformes)
Diniz-Filho, José Alexandre Felizola;Sant'Ana, Carlos Eduardo Ramos de;
Genetics and Molecular Biology , 2000, DOI: 10.1590/S1415-47572000000200008
Abstract: during the last few years, many models have been proposed to link microevolutionary processes to macroevolutionary patterns, defined by comparative data analysis. among these, brownian motion and ornstein-uhlenbeck (o-u) processes have been used to model, respectively, genetic drift or directional selection and stabilizing selection. these models produce different curves of pairwise variance between species against time since divergence, in such a way that different profiles appear in phylogenetic correlograms. we analyzed variation in body length among 19 species of south american owls, by means of phylogenetic correlograms constructed using moran's i coefficient in four distance classes. phylogeny among species was based on dna hybridization. the observed correlogram was then compared with 500 correlograms obtained by simulations of brownian motion and o-u over the same phylogeny, using discriminant analysis. the observed correlogram indicates a phylogenetic gradient up to 45 mya, when coefficients tend to stabilize, and it is similar to the correlograms produced by the o-u process. this is expected when we consider that body size of organisms is correlated with many ecological and life-history traits and subjected to many constraints that can be modeled by the o-u process, which has been used to describe evolution under stabilizing selection.
Expression of the cell cycle regulation proteins p53 and p21WAF1 in different types of non-dysplastic leukoplakias
Visioli, Fernanda;Lauxen, Isabel Silva;Sant'Ana Filho, Manoel;Rados, Pantelis Varvaki;
Journal of Applied Oral Science , 2012, DOI: 10.1590/S1678-77572012000300013
Abstract: objectives: the aim of this study was to analyze the immunolabeling of two cell cycle protein regulators, p53 and p21waf1, in non-dysplastic leukoplakias with different epithelial alterations: acanthosis, hyperkeratosis and acanthosis combined with hyperkeratosis, and compare them with dysplastic leukoplakias. material and methods: this was a prospective cohort study involving 36 patients with oral homogeneous leukoplakias. excisional biopsies were performed and the patients remain under clinical follow-up. the leukoplakias were divided into four groups: 6 acanthosis, 9 hyperkeratosis, 10 acanthosis combined with hyperkeratosis, and 11 epithelial dysplasias. paraffin-embebeded sections were immunostained for p53 and p21waf1. five hundred cells from the basal layer and 500 from the parabasal layer were counted to determine the percentage of positive cells. a qualitative analysis was also carried out to determine the presence or absence of immunohistochemical staining in the intermediate and superficial layers. groups were compared with anova (p<0.05). pearson's correlation coefficient was used to test for associations between the two markers, p53 and p21waf1. results: no leukoplakia recurred and no malignant transformation was observed whitin a follow-up period of 3-6 years. the mean percentage of p53 staining in the basal and parabasal layers was similar in all groups. p21waf1 staining differed between layers was as follows: in the basal, only 3 to 4% of cells were stained, while in the parabasal, between 16 and 28% of the epithelial cells were stained in the four different studied groups with no statistically significant difference (p>0.05). conclusions: our findings failed to differentiate the non-dysplastic lesions by means of p53 and p21waf1 immunostaining, notwithstanding similar profiles between non-dysplastic and dysplastic leukoplakias were observed.
Psicólogo e escola: a compreens?o de estudantes do ensino fundamental sobre esta rela??o
Sant'Ana, Izabella Mendes;Euzébios Filho, Antonio;Lacerda Junior, Fernando;Guzzo, Raquel Souza Lobo;
Psicologia Escolar e Educacional , 2009, DOI: 10.1590/S1413-85572009000100004
Abstract: this study investigates how students from an elementary public school in campinas, s?o paulo, brazil, perceived the psychologist’s role at school. the information was obtained from drawings and writings carried out by 127 students in their initial grades, besides 113 students in their final grades answered two questions about the work of the psychologist. the results indicated that in general students perceived the psychologist as a professional who talks about the aspects of () life and gives support to the school community, acting as a mediator of the social interactions in this context. the final-grade students’ expectations about the work of the psychologist involved: guidance, help to school and their members to solve problems and active participation in school daily life. the results showed a positive tendency towards the inclusion of a psychologist in school, according to students, despite the limited understanding of her or his role.
Otimiza??o da técnica de citometria de fluxo para análise do fator tissular em monócitos de sangue periférico
Vieira, Lauro M.;Dusse, Luci M. Sant'Ana;Martins Filho, Olindo A.;Carvalho, Maria G.;
Revista Brasileira de Hematologia e Hemoterapia , 2003, DOI: 10.1590/S1516-84842003000300004
Abstract: the development of the flow cytometric assay using monoclonal antibodies labeled with different fluorescent substances enables the identification of a particular cell population even if it is present in heterogeneous cell samples. this technique has been applied to oncohematology and bone marrow transplantation studies. two combined fluorescent monoclonal antibodies enable the study of a particular cell population in the expression of procoagulant substances produced by cells involved in homeostatic mechanisms. the application of this methodology creates the perspective of pathophysiologic studies related to hypercoagulability states. considering that monocytes are highly involved in pathophysiologic mechanisms contributing to thrombus formation and atheromatous plaques and that tissue factor represents the principal physiologic activator of the clotting system, this study constitutes a potential tool for obtaining new insights of the role of monocytes in diseases associated to hypercoagulability states. the present work aimed to establish the optimization of conditions for measuring the tissue factor expression in monocytes stimulated or not by lipopolysaccharides from escherichia coli and analyzed by flow cytometric assay based on a previous methodology. blood samples were collected from healthy subjects and divided in two age ranges. studies on monocytes were carried out comparing two methods of analysis, which define the percentage of cells expressing tissue factor on their surface. from the results, it was concluded that there is no difference between the two age ranges related to the tissue factor expression in monocytes. in addition, there were no significant differences between the two assessed methods of analysis.
Otimiza o da técnica de citometria de fluxo para análise do fator tissular em monócitos de sangue periférico
Vieira Lauro M.,Dusse Luci M. Sant'Ana,Martins Filho Olindo A.,Carvalho Maria G.
Revista Brasileira de Hematologia e Hemoterapia , 2003,
Abstract: A citometria de fluxo permite a análise individual de células quando há express o de moléculas de membrana, produtos citoplasmáticos e nucleares. Quando se utiliza a marca o prévia das células com anticorpos monoclonais é possível estudar constituintes de membrana característicos de uma determinada linhagem celular. Assim, esta metodologia tem sido largamente utilizada, principalmente na área de onco-hematologia e dos transplantes de medula óssea. A possibilidade de se utilizarem dois ou mais anticorpos monoclonais marcados com fluorocromos diferentes permite o estudo em uma determinada popula o celular de substancias pró-coagulantes produzidas por células envolvidas nos mecanismos da hemostasia, abrindo-se a possibilidade de aplica o desta metodologia em áreas de estudo da fisiopatologia relacionada aos estados de hipercoagulabilidade. Os monócitos est o altamente envolvidos nos processos fisiopatológicos envolvendo a forma o de trombo e placas ateromatosas, e o Fator Tissular (FT) consiste no principal ativador fisiológico do sistema de coagula o sangüínea. Monócitos estimulados ou n o por lipopolissacárides de Escherichia coli expressam FT em condi es fisiológicas normais. Neste trabalho buscou-se otimizar as condi es de determina o da express o de FT em monócitos por citometria de fluxo, a partir de metodologia já descrita, em duas popula es de indivíduos sadios, com faixas etárias diferentes, no sentido de se estabelecerem os níveis de express o de FT. Para a análise dos resultados foram utilizadas duas metodologias que definem o percentual de células expressando FT. Dos resultados obtidos, pode-se concluir que n o há diferen a significativa na express o de FT de monócitos em fun o da idade e que os dois métodos de análise utilizados n o diferem entre si.
Phylogenetic correlograms and the evolution of body size in South American owls (Strigiformes)
Diniz-Filho José Alexandre Felizola,Sant'Ana Carlos Eduardo Ramos de
Genetics and Molecular Biology , 2000,
Abstract: During the last few years, many models have been proposed to link microevolutionary processes to macroevolutionary patterns, defined by comparative data analysis. Among these, Brownian motion and Ornstein-Uhlenbeck (O-U) processes have been used to model, respectively, genetic drift or directional selection and stabilizing selection. These models produce different curves of pairwise variance between species against time since divergence, in such a way that different profiles appear in phylogenetic correlograms. We analyzed variation in body length among 19 species of South American owls, by means of phylogenetic correlograms constructed using Moran's I coefficient in four distance classes. Phylogeny among species was based on DNA hybridization. The observed correlogram was then compared with 500 correlograms obtained by simulations of Brownian motion and O-U over the same phylogeny, using discriminant analysis. The observed correlogram indicates a phylogenetic gradient up to 45 mya, when coefficients tend to stabilize, and it is similar to the correlograms produced by the O-U process. This is expected when we consider that body size of organisms is correlated with many ecological and life-history traits and subjected to many constraints that can be modeled by the O-U process, which has been used to describe evolution under stabilizing selection.
15N-labed glycine synthesis
Tavares Claudinéia R.O.,Bendassolli José A.,Coelho Fernando,Sant'ana Filho Carlos R.
Anais da Academia Brasileira de Ciências , 2006,
Abstract: This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used (50, 100, and 150 mL), in order to calibrate the best operational condition; glycine masses obtained were 1.7, 2, and 3.2 g, respectively. With the development of a system for 15NH3 recovery, it was possible to recover 71, 83, and 87% of the ammonia initially used in the synthesis. With the required adaptations, the same system was used to recover methanol, and 75% of the methanol initially used in the amino acid purification process were recovered.
Avalia o do uso de terra de diatomácea contra a infesta o de gr os de milho Evaluation of the use of diatomaceous earth against the corn grains infestation
Luidi E. G. Antunes,Edar Ferrari Filho,Roberto Gottardi,Josué SantAna
Revista Brasileira de Ciências Agrárias , 2011, DOI: 10.5039/agraria.v6i4a1486
Abstract: Estima-se que s o perdidos 10% do total produzido na pós-colheita de gr os no Brasil com o ataque de pragas. Entre os principais insetos que atacam produtos armazenados, destacam-se as espécies Sitophilus zeamais e Tribolium castaneum, que podem ser controladas com terra de diatomácea. Objetivou-se verificar a eficiência de diferentes dosagens de terra de diatomácea no controle de adultos de S. zeamais e T. castaneum em gr os de milho durante o armazenamento. Utilizaram-se gr os de milho com 12, 14 e 16% de umidade, com 92,35% de gr os inteiros, e doses de 1000 e 2000 g t-1 de terra de diatomácea. Cada tratamento foi composto por cinco repeti es com 100 g de gr os tratados, ou n o no caso do controle, infestados com 10 adultos de ambas as espécies. Ocorreram três períodos de infesta es: 1 hora, 10 dias e 20 dias após a aplica o do produto. Verificou-se a mortalidade dos insetos e a análise tecnológica dos gr os aos 60 dias após cada período de infesta o. Maiores médias de mortalidade (100%) foram obtidas no menor teor de umidade (12%) e maiores níveis de gr os carunchados (44,15% e 24,55%) nos tratamentos controle dos gr os com os maiores teores de umidade (14 e 16%, respectivamente). Conclui-se, assim, que o tratamento com terra de diatomácea é eficiente para o controle de S. zeamais e T. castaneum, reduzindo os danos nos gr os de milho, especialmente com teor de umidade em torno de 12%. Grains postharvest losses in Brazil are estimated to be about 10%, mainly due to insects infestation. The species Sitophilus zeamais and Tribolium castaneum, which can be controlled by diatomaceous earth, are the principal insects infesting stored products. The objective of this study was to determine the effectiveness of different diatomaceous earth doses on the control of adult S. zeamais and T. castaneum in stored corn grains. Corn grains at 12, 14 and 16% humidity, with 92.35% of whole grain, were treated with 1000 and 2000 g t-1 doses of diatomaceous earth. Each treatment was composed by five replications with 100 g of treated grains, or not (control), infested with 10 adult insects of each species. Three infestation periods were observed: 1 hour, 10 days and 20 days after the application of the product. The insect mortality and the technological grain analysis were verified 60 days after each infestation period. Higher mortality means (100%) were obtained with the lowest (12%) humidity content, and higher rotten grain levels (44.15% and 24.55%) were observed in control treatments of grains with the highest humidity contents (14 and 16%, respectively). Therefo
Distribution of CD8 and CD20 lymphocytes in chronic periapical inflammatory lesions
Philippi Christine Kalvelage,Rados Pantelis Varvaki,Sant'ana Filho Manoel,Barbachan Jo?o Jorge Diniz
Brazilian Dental Journal , 2003,
Abstract: The objective of this study was to investigate the distribution of CD8+ and CD20+ lymphocytes in chronic periapical inflammatory lesions. A total of 90 periapical inflammatory lesions (chronic abscesses, abscessed cysts, and inflammatory cysts) were evaluated. The biotin-streptavidin immunohistochemical technique was used to identify cytotoxic/suppressor T-lymphocytes (CD8) and B-lymphocytes (CD20). Age ranged from 10 to 67 years. Patients between 26 and 45 years old (54.4%), females (52.2%), and white patients (74.4%) were more frequently affected. CD8+ cell distribution was as follows: 1) fibrous capsule: diffuse in 58.8% of chronic abscesses and absent in 64.1% of abscessed cysts and in 70.6% of inflammatory cysts; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 82.4% of inflammatory cysts; 3) sub-epithelial zone: absent in 53.0% of inflammatory cysts and diffuse in 56.4% of abscessed cysts; 4) suppurative zone: diffuse in 100% of chronic abscesses and in 97.5% of abscessed cysts. CD20+ cell distribution was as follows: 1) fibrous capsule: absent in 100% of inflammatory cysts, in 94.8% of abscessed cysts, and in 88.3% of chronic abscesses; 2) infiltration zone: diffuse in 100% of abscessed cysts and in 53% of inflammatory cysts; 3) sub-epithelial zone: absent in 58.8% of inflammatory cysts and focal in 46.2% of abscessed cysts; 4) suppurative zone: diffuse in 100% of abscessed cysts and in 100% of chronic abscesses. The distribution of the lymphocytic infiltrate in the lesions was usually diffuse for both types of lymphocytes.
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