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Search Results: 1 - 10 of 7102 matches for " Salcedo-Morales "
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Propagation and conservation of Castilleja tenuiflora Benth. ("hierba del cancer") through in vitro culture
Salcedo-Morales, Guadalupe;Rosas-Romero, Gabriel;Nabor-Correa, Nayeli;Bermúdez-Torres, Kalina;López-Laredo, Alma R.;Trejo-Tapia, Gabriela;
Polibotánica , 2009,
Abstract: we undertook this study to (1) evaluate an in vitro procedure for plantlet regeneration of castilleja tenuiflora benth. (scrophulariaceae) from axillary buds and (2) induce callugenesis and organogenesis through the manipulation of explant type, culture media and plant growth regulators. an efficient propagation protocol for in vitro multiplication and plantlet regeneration of c. tenuiflora using axillary buds of wild plants was developed. shoot multiplication was induced from axillary buds in murashige and skoog (ms) medium containing 0.2 mg l-1 bap and 0.1 mg l-1 naa with an efficiency of 33%. shoot multiplication and elongation were achieved in one step using 0.1 mg l-1 iba and 0.25 mg l-1 bap. after 14 days, an average of four shoots per explant was observed. for rooting, iba was increased to 1.0 mg l-1 and bap was excluded. hyperhydricity was not observed and 88% of the shoots rooted. from one axillary bud, 250 plantlets were produced within eight weeks. to induce callugenesis and organogenesis, explants (leaves and internodes) from plantlets were excised and inoculated into ms, b5 and nn culture media in combination with naa (0-10 μm) and kinetin (0-0.5 μm). in general, rhizogenesis was the main in vitro response (up to 100%) followed by shoot formation (5-50%) and, finally, callugenesis (2-35%). internodes were more competent than leaves for both callugenesis and organogenesis, along with the fact that leaf explants oxidized easily. rhizogenesis depended on exogenous naa, but auxin requirement varied according to the culture medium and type of explant used. on the basis of our results, conditions for callugenesis and organogenesis induction of c. tenuiflora can be recommended: a) callus-internode, 0.1 μm naa and b5 medium; b) rhizogenesis-0.1 μm naa and nn medium; and c) shoots-internode, 0.1 μm naa and ms medium. results of the present study show the feasibility of using in vitro culture to propagate and conserve germplasm of the 'cancer herb' c. tenuiflora.
Densidad estomatal y potencial hídrico en plantas de tomate (Physalis ixocarpa Brot.), propagadas por cultivo de meristemos
Bazaldúa-Mu?oz, C.;Ventura-Zapata, E.;Salcedo-Morales, G.;Maldonado Amaya, U.;López García, A.;
Revista Chapingo. Serie horticultura , 2008,
Abstract: husk tomato is a solanaceous plant from mexico, this plant can grow wild or cultivated. in vitro propagation is an alternative for mass production of pathogen free plants. but some times acclimatization has been the limiting factor to reach this objective. few reports related with husk tomato micropropagation are found, and this is the first one kwnown to us where the changes on stomatal density and water potential and plantlets obtained from apical meristems during the acclimatization, are evaluated. that evaluation was done during the acclimatization from plantlets obtained by mean of apical meristem culture. plants of 30 days old with similar size were placed in individual hydroponics system, during seven weeks, all of them with plastic covers, which were gradually opened each seven days. each time, the temperature and relative humidity were measured, in the acclimatization system and the growth chamber, for water potential estimation. at the sixth week, the water potential in the acclimatization system was close to that of the growth chamber (-107.8 and -102.2 ± 4.91 respectively). on the other hand, the stomatal density was evaluated at 15, 30 and 45 days of the acclimatization stage. significant differences were observed at this parameter between adaxial and abaxial leaves surfaces, and between different plant ages. when both, water potential and stomatal density data were compared, a great correlation was observed. a 100% of the plants survived the acclimatization period.
Establecimiento de un protocolo de permeabilización con detergentes que incrementa la eficiencia en la producción de betacianinas en cultivos de células de Beta vulgaris L
Salcedo-Morales,Guadalupe; Trejo-Tapia,Gabriela; Martínez-Bonfil,Blanca P; De Jesús-Sánchez,Antonia; Arenas-Ocampo,Martha L; Jiménez-Aparicio,Antonio R;
Interciencia , 2009,
Abstract: red beet (beta vulgaris l.) cell suspensions were permeabilized by means of four chemical detergent agents, tween 20, 40 and 80, and triton x-100?, to evaluate the recovery of betacianins (bc). the permeabilizating agent was selected as a function of the quantity of bc released and the contact time. betacianin concentration was measured using digital color image analysis. the results showed that 36% of betacianins was released using triton x-100? (0.7mm) during 10min; using these extraction conditions, the viability remained at 60-70%. this treatment allowed a second growing-cycle, as well as, an additional accumulation of betacianins.
"FACULTAD DISCRECIONAL", PLANEACIóN Y EFICIENCIA EN LA GESTIóN DEL RECURSO HUMANO MILITAR
Javier G Rincón Salcedo,Edna M Morales Vargas
Prolegómenos. Derechos y Valores , 2009,
Abstract: El ejercicio de la Facultad Discrecional como mecanismo legal otorgado al ejecutivo para que pueda retirar por "Razones del Servicio" a funcionarios Militares, ha sido objeto de importantes delimitaciones por parte de los jueces administrativos y constitucionales. Estas delimitaciones, lejos de constituir barreras para el ejercicio de la actividad de la administración dentro de un país en conflicto, deben ser consideradas, por una parte, como mecanismos que, por la vía de considerar que las Actas de evaluación son parte del Acto Administrativo de Retiro, permiten garantizar el Derecho de Defensa de los funcionarios militares, y por otra parte, como instrumentos que contribuyen a "administrar mejor", en la medida que ejercen una presión sobre la Administración para que esta implemente una política coherente de Recursos humanos, que, naturalmente, redundará en el desarrollo de una gestión más eficiente del Estado.
Fístula pancreatogastrocutánea: presentación comentada de un caso
González Salcedo,Joel; Pita Armenteros,Luis; Pérez Jomarrón,Emilio; Morales Díaz,Ignacio;
Revista Cubana de Cirug?-a , 2010,
Abstract: this is the case of a heavy drinker patient aged 39, operated on with urgency due to acute appendicitis; during the operation we found a necrotic-hemorrhagic acute pancreatitis and extensive peritonitis. a programmed peritoneal lavage in wall by plans; afterwards he had fever and it was necessary to drain a subaponeurotic abscess. there was a weight loss and a suppuration by residual orifice in drainage made. a catheter was passed through the orifice for irrigation, secretion cleared and then a fluid of possible intestinal origin flowed. the fistulography showed the existence of a communication between the stomach and the left hypochondrium, obliged us to a reintervention. the patient suffered a hemoperitoneum, and thus a second laparoscopy was performed to carry out a splenectomy in the immediate postoperative period. patient felt well although there was weight loss, tachycardia without fever and computed axial tomography (cat) showed a intracavitary collection in left hypochondrium, draining by transparietal route.
Fecaloma, oblito y oclusión intestinal: informe de un caso, comentarios y una propuesta
Pita Armenteros,Luis; Pérez Jomarrón,Emilio; González Salcedo,Joel; Morales Díaz,Ignacio;
Revista Cubana de Cirug?-a , 2010,
Abstract: this is a case presentation of a patient aged 47 with a huge right humpback appearing at 18 years old. he was operated on 14 years ago from a cystic formation increasing the volume of limbs and the appearance afterwards of a 2,5 cm tumor. ten days before its admission showed a urinary infection, constipation, difficulty to release gases, pain and abdominal distention, visualizing such tumor. at radiography on noted many fecaloma and metallic foreign body. he was operated on verifying the presence of fecalomas, loop distention and foreign body whose tip was visualized and palpated.
Vía lumbar para plastrón apendicular abscedado
Pita Armenteros,Luis; González Salcedo,Joel; Pérez Jomarrón,Emilio; Morales Díaz,Ignacio;
Revista Cubana de Cirug?-a , 2010,
Abstract: this is a case presentation of a woman aged 32 with a torpid course from the onset of her disease. she was operated on due to an abscess appendicular plastron. despite two surgical interventions there were weak signs in right lumbar zone. a lumbotomy was performed and in abscess cavity in retroperitoneum an indwelling catheter was placed for fast dripping clysis during the postoperative period. then, this cavity was roofed off for second-look closure.
Vía lumbar para plastrón apendicular abscedado Lumbar route use for abscess appendicular plastron
Luis Pita Armenteros,Joel González Salcedo,Emilio Pérez Jomarrón,Ignacio Morales Díaz
Revista Cubana de Cirugía , 2010,
Abstract: Se presenta el caso de una paciente de 32 a os de edad, con evolución tórpida desde el inicio de su enfermedad. Se intervino quirúrgicamente por presentar un plastrón apendicular abscedado. A pesar de que requirió 2 intervenciones, aparecieron signos flogísticos en la región lumbar derecha. Se practicó una lumbotomía y en la cavidad del absceso en el retroperitoneo se dejó un catéter para clisis de goteo rápido durante el posoperatorio. Luego se destechó dicha cavidad para cierre por segunda intención. This is a case presentation of a woman aged 32 with a torpid course from the onset of her disease. She was operated on due to an abscess appendicular plastron. Despite two surgical interventions there were weak signs in right lumbar zone. A lumbotomy was performed and in abscess cavity in retroperitoneum an indwelling catheter was placed for fast dripping clysis during the postoperative period. Then, this cavity was roofed off for second-look closure.
Fístula pancreatogastrocutánea: presentación comentada de un caso Pancreatogastrocutaneous fistula: a discussed case presentation
Joel González Salcedo,Luis Pita Armenteros,Emilio Pérez Jomarrón,Ignacio Morales Díaz
Revista Cubana de Cirugía , 2010,
Abstract: Se presenta el caso de un paciente de 39 a os, bebedor inveterado, intervenido de urgencia por apendicitis aguda, y en dicha operación se encontró pancreatitis aguda necrótico-hemorrágica y peritonitis extensa. Se realizó lavado peritoneal programado y síntesis de la pared por planos; más tarde presentó fiebre y fue necesario drenar un absceso subaponeurótico. Hubo pérdida de peso y apareció supuración por el orificio residual del drenaje dejado. Se pasó un catéter por dicho orificio para irrigación, la secreción aclaró y más tarde salió líquido de posible origen intestinal. La fistulografía demostró una comunicación entre el estómago y el hipocondrio izquierdo, que obligó a una reintervención. El paciente sufrió un hemoperitoneo y se realizó una relaparotomía para esplenectomía en el posoperatorio inmediato. Después estuvo bien, aunque continuó la pérdida de peso. Luego presentó taquicardia sin fiebre y la tomografía axial computarizada mostró una colección intracavitaria en el hipocondrio izquierdo, la cual fue drenada por vía transparietal. This is the case of a heavy drinker patient aged 39, operated on with urgency due to acute appendicitis; during the operation we found a necrotic-hemorrhagic acute pancreatitis and extensive peritonitis. A programmed peritoneal lavage in wall by plans; afterwards he had fever and it was necessary to drain a subaponeurotic abscess. There was a weight loss and a suppuration by residual orifice in drainage made. A catheter was passed through the orifice for irrigation, secretion cleared and then a fluid of possible intestinal origin flowed. The fistulography showed the existence of a communication between the stomach and the left hypochondrium, obliged us to a reintervention. The patient suffered a hemoperitoneum, and thus a second laparoscopy was performed to carry out a splenectomy in the immediate postoperative period. Patient felt well although there was weight loss, tachycardia without fever and computed axial tomography (CAT) showed a intracavitary collection in left hypochondrium, draining by transparietal route.
Fecaloma, oblito y oclusión intestinal: informe de un caso, comentarios y una propuesta Fecaloma, foreign body and intestinal occlusion: a care report, comments and a proposal
Luis Pita Armenteros,Emilio Pérez Jomarrón,Joel González Salcedo,Ignacio Morales Díaz
Revista Cubana de Cirugía , 2010,
Abstract: Se presenta el caso de un paciente de 47 a os con enorme giba lumbar derecha que apareció a los 18 a os de edad. Fue operado hace 14 a os de una formación quística con aumento de volumen de las extremidades y aparición, poco después, de un tumor de 2,5 cm. Diez días antes de su ingreso presentó infección urinaria, luego constipación, dificultad para expulsar gases, dolor y distensión abdominal, y se visualizaba la referida tumoración. En la radiografía se observaron múltiples fecalomas y oblito metálico. Fue operado y se constató la presencia de fecalomas, distensión de asas y el oblito cuya punta era la que se visualizaba y palpaba. This is a case presentation of a patient aged 47 with a huge right humpback appearing at 18 years old. He was operated on 14 years ago from a cystic formation increasing the volume of limbs and the appearance afterwards of a 2,5 cm tumor. Ten days before its admission showed a urinary infection, constipation, difficulty to release gases, pain and abdominal distention, visualizing such tumor. At radiography on noted many fecaloma and metallic foreign body. He was operated on verifying the presence of fecalomas, loop distention and foreign body whose tip was visualized and palpated.
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