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Epidemiología y bioinformática en el estudio de la Leucemia Linfoma de Células T del Adulto asociada a la infección con VLHT-1
Salcedo-Cifuentes,Mercedes; Restrepo,Oscar; García-Vallejo,Felipe;
Revista de Salud Pública , 2011, DOI: 10.1590/S0124-00642011000100011
Abstract: objectives establishing a correlation between the number of htlv-1 provirus and the characteristics of the genomic environment in atl cases. methodology a systematic search was made of publications as well as a meta-analysis of the pertinent literature considering proviruses per chromosome and structural and functional characteristics of flanking chromatin regions as variables. the concordance of experts' study was evaluated by spearman rho correlation. publication bias was analysed by funnel plot and the egger statisgrapher. a fixed effects model was applied according to heterogeneity evaluation to combine the results of integration occurring in coding sequences as well as coding sequences according to their molecular function. results the expert concepts' kappa index was 0.7 and no publication bias was observed. the meta-analysis result was homogeneous (p>0.05). htlv-1 integration was directed towards several chromosomes' telomeric and subtelomeric regions. the combination of published results in the articles which were analysed supported the hypothesis of integration events being site-directed towards coding regions of the human genome (p<0.05). moreover, the groups of genes having enzymatic and receptor functions was statistically significant. conclusion the results led to concluding that htlv-i integration in the atll cases analysed here was not random but was directed towards regulatory regions. such results could help to explain the role of some processes involved in leukemogenesis.
Violencia sexual en jóvenes de 10 a 19 a?os. Cali, 2001-2006
Restrepo-Gutiérrez,Ana C.; Salcedo-Cifuentes,Mercedes; Bermúdez-Escobar,Amparo;
Revista de Salud Pública , 2009, DOI: 10.1590/S0124-00642009000600005
Abstract: objective characterising sexual violence reported by youngsters aged 10 to 19 in cali between 2001 and 2006. components and methods young people aged 10 to 19 who had been referred to a forensic clinic by a competent authority between 2001 and 2006 were defined as being presumed victims of sexual crimes. variables of time, place and personsubjected to sexual crime committed by a family member (intrafamily), an acquaintance or unknown public individual (extrafamily) were compared. there were 661 cases of presumed victims of sexual abuse from both sexes in the age-range being studied. the information was taken from the forensic clinic's patient records. chi square and relative risk were used in the statistical analysis of the data so collected concerning frequency for presumed sexual victims aged 10 to 19 and analysing the relationships between variables. results 83 % of the cases in the study were female, 78 % were students and more than 50 % were 15 year-old minors. the aggressors were known in 78 % of the cases. a significant association was found between involvement with the aggressor and the place where the incident occurred with a victim's age. conclusions the characteristics of sexual violence occurring in adolescents were constant and comparable with historical studies where family dynamics and organisation made such scene opportune for this type of violent act to be perpetrated.
Epidemiología genómica y paraparesia espástica tropical asociada a la infección por el virus linfotrópico humano de células T tipo 1
Salcedo-Cifuentes,Mercedes; Domínguez,Martha C; García-Vallejo,Felipe;
Revista Panamericana de Salud Pública , 2011, DOI: 10.1590/S1020-49892011001100004
Abstract: objective: characterize the genomic environment of the sequences adjacent to human t-cell lymphotropic virus type 1 (htlv-1) in patients with htlv-1-associated myelopathy/tropical spastic paraparesis (ham/tsp) in different regions of colombia and japan. methods: a total of 71 recombinant clones with human genome sequences adjacent to 5' ltr in patients with ham/tsp were compared to the genome browser and genbank databases. sixteen structural and compositional genome variables were identified, and statistical analysis was conducted in the r computer program, version 2.8.1, in a 0.5 mb window. results: a total of 43.0% of the proviruses were located in the group c chromosomes; 74% of the sequences were located in the telomeric and subtelomeric regions (p < 0.05). a cluster analysis was used to establish the hierarchical relations between the genome characteristics included in the study. the analysis of principal components identified the components that defined the preferred genome environments for proviral integration in cases of ham/tsp. conclusions: htlv-1 was integrated more often in chromatin regions rich in cpg islands with a high density of genes and line type repetitions, and dna transposons which, overall, would form the genomic environments targeted for integration. this new scenario will promote substantial changes in the field of public health and in epidemiological management of infectious diseases. it will also foster the development of powerful tools for increasing the efficiency of epidemiological surveillance
Expansión clónica y caracterización genómica del proceso de integración del virus linfotrópico humano tipo I en la leucemia/linfoma de células T en adultos Clonal expansion and genomic characterization of the human T-cell lymphotropic virus type I during the integration process in adult T-cell leukemia/lymphoma
Mercedes Salcedo-Cifuentes,Jesús Cabrera,Yesid Cuesta-Astroz,Edwin Carrascal
Biomédica , 2009,
Abstract: Introducción. Aunque la integración del virus linfotrópico humano tipo I no es al azar, se desconocen muchos de los detalles de este proceso. Objetivo. Evaluar las características de la cromatina celular adyacente a secuencias provirales en pacientes con leucemia/linfoma de células T en adultos asociada al virus. Materiales y métodos. Se extrajo el ADN de biopsias de siete pacientes colombianos con leucemia/linfoma de células T en adultos y positivos para el virus linfotrópico humano tipo I. éste se amplificó mediante reacción inversa en cadena de la polimerasa, para determinar el grado de expansión clónica y su composición de nucleótidos. A partir de 61 secuencias de ADN humano adyacentes a provirus, provenientes de pacientes leucémicos colombianos y japoneses, se efectuó un análisis in silico para obtener datos sobre su integración, las características de la cromatina y sus funciones asociadas. Resultados. La expansión de clones celulares fue predominantemente oligoclónica. De las 61 secuencias de ADN adyacente a provirus, se seleccionaron 155 alineamientos que cumplieron con los criterios de inclusión (homologías≥95%, e-value≤0,05). De éstos, 74,84% fueron secuencias no codificantes repetidas y no repetidas. El 45,95% de las integraciones provirales se localizó en los cromosomas de los grupos A y B. Se observaron tendencias de integración hacia exones de genes que se replican tempranamente, regulan el ciclo celular y participan en la transducción de se ales. Conclusiones. Los resultados permiten postular que la integración del virus linfotrópico humano tipo I se dirigiría hacia un ambiente genómico caracterizado por elevado contenido de C:G, genes de replicación temprana que regularían el ciclo celular y la transducción de se ales. Introduction. Although the integration of human T-cell lymphotropic virus type I into the T-cells is not a random process, the mechanistic details are not understood. Objectives. The characteristics of the flanking host chromatin were evaluated at the integration sites in adult T-cell leukaemia/lymphoma (ATLL) patients infected with the virus. Materials and methods. From seven leukemic Colombian patients positive for the human T-cell lymphotropic virus type I (HTLV-I), lymphocyte DNA samples were extracted and amplified by inverse polymerase chain reaction (IPCR). Clonal expansion and human genome nucleotide composition in an extension of 50 bp was determined. To establish the characteristics of the human genome flanking provirus, 61 IPCR sequences from Colombian and Japanese ATLL patients, were analyzed in silico to obtain
Expansión clónica y caracterización genómica del proceso de integración del virus linfotrópico humano tipo I en la leucemia/linfoma de células T en adultos
Salcedo-Cifuentes,Mercedes; Cabrera,Jesús; Cuesta-Astroz,Yesid; Carrascal,Edwin; Eizuru,Yoshito; Domínguez,Martha C; Sánchez,Adalberto; García-Vallejo,Felipe;
Biomédica , 2009,
Abstract: introduction. although the integration of human t-cell lymphotropic virus type i into the t-cells is not a random process, the mechanistic details are not understood. objectives. the characteristics of the flanking host chromatin were evaluated at the integration sites in adult t-cell leukaemia/lymphoma (atll) patients infected with the virus. materials and methods. from seven leukemic colombian patients positive for the human t-cell lymphotropic virus type i (htlv-i), lymphocyte dna samples were extracted and amplified by inverse polymerase chain reaction (ipcr). clonal expansion and human genome nucleotide composition in an extension of 50 bp was determined. to establish the characteristics of the human genome flanking provirus, 61 ipcr sequences from colombian and japanese atll patients, were analyzed in silico to obtain insights about the genomic structure, functions and nature of associated chromatin. results. the clonal expansion of cell clones was predominantly oligoclonal. from 61 ipcr sequences, 155 alignments with homology higher than 95% (e-value <0.05) were screened. seventy-five percent of those sequences corresponded to non coding elements that include repetitive and non-repetitive dna. fifty percent of the proviral integrations were associated with chromosomes of a and b groups. viral dna integration tended to favor exons of genes that replicated early, controlled the cell cycle, or were involved in signal transduction. conclusions. the results indicated that htlv-i integration was preferentially directed towards genomic environments with high c:g content, and toward genes that replicate early, regulate cell cycle or involved with signal transduction.
Modelación molecular y variación estructural de las integrasas de dos retrovirus humanos: HTLV-I y VIH-1
García Vallejo,Felipe; Cuesta Astroz,Yesid; Domínguez,Martha C; Sánchez,Adalberto; Salcedo Cifuentes,Mercedes; Díaz,Diane María; Quintana,Milton;
Revista Salud Uninorte , 2009,
Abstract: objective: to analyze the molecular characteristics and amino acid sequence variations of htlv-i and of hiv-1 integrases and their population variants. materials adn methods: data mining and analysis of integrase sequences and protein structure data bases by using appropriate software for modelling and search for polymorphic substitutions in htlv-i and hiv-1 integrase amino acid sequences previously reported. results: htlv-i and hiv-1 integrases are proteins of 288 amino acid residues. structural modeling of tertiary folding of htlv-i integrase catalytic central domain’s, showed closed structural characteristic with those of hiv-1, asv and rsv. from 103 full amino acid sequences of hiv-1 integrase, 53 substitutions located in 46 different codons were recorded. the more frequents correspond to n27g (32,1%), l101i (31,1%), a265v (30,1%) and t123a (27,0%). none of these frequent substitutions introduced changes in the folding of hiv-1 native integrase. conclusion: the tridimensional structure of central catalytic domain would influence the integrase activity and its relationship with potentially inhibitory molecules. those observed amino acid substitutions were neutral and do not alter the native protein structure. our data confirm those previously published, and enable us to propose that in is a new and promissory target for develop more effective antiviral therapies in the xxi century.
Subjectivities of Young People Demobilized from FARC in a Scenario of Peace Construction  [PDF]
Luz Marina Lara Salcedo
Open Journal of Political Science (OJPS) , 2017, DOI: 10.4236/ojps.2017.71007
Abstract: This article presents the results of a research on the displacement of subjectivities of a group of demobilized young people formerly enlisted in the Colombian armed group FARC guerrillas (Fuerzas Armadas Revolucionarias de Colombia, Revolutionary Armed Forces of Colombia). Drawing on a social constructionist perspective (Gergen, 1996), this paper emphasizes a perspective of the subject formed inside the armed group—under new conditions, scenarios, personals traits and ways of living—, as well as the new subject that emerges after deciding to rejoin civilian life. The methodological design was oriented from the central premises of the social constructionist perspective. The methodological approach was the narrative inquiry with its three phases: production, analysis and interpretation. For analytical purposes, the model used was the narrative approach proposed by Coninck & Godard (1998). The main results show that when the youngsters decide leaving the armed group, begins the transition to civilian life; it is a time of confrontation, doubts and fears that young people must face in their own way. The youngsters came from being immersed in an armed group that supported their identity. The transition implies losing a way of living, experiencing the uncertainty, but also with the hope of rebuilding their life projects. In this transit, youngsters feel unprotected and helpless; the values acquired of brotherhood and solidarity in the guerrilla life suddenly disappear and young people is confronted with a world of new social meanings and ways of being and doing. The results show the rupturing and continuities that rise in their subjectivities before and after joining the armed group, as well as the emerging and conflicting subjectivities in the process of disengagement. In addition, great fluctuations of emotional repertoires, as well as identity, ties, values and lifestyles go through a process of redefinition and reconfiguration to settle back into civilian society.
El colapso del Puente Samaná: un nuevo enfoque
Cifuentes Cifuentes Gustavo
Ingeniería e Investigación , 1997,
Abstract: E13 de octubre de 1993, colapsó la superestructura del puente sobre el río Samaná en la carretera Bogotá - Medellín, en Colombia. La Facultad de Minas de la Universidad Nacional, en Medellín, llevó a cabo un estudio que se ala como causa probable del colapso, la falla de la unión de la armadura inferior con un pendolón. Un nuevo estudio analítico realizado en 1996, por la Facultad de Ingeniería de la Universidad Nacional, en Bogotá, indica que el puente tenía problemas de estabilidad general, los cuales se presentan en este artículo, y se indican como posible causa del colapso. Así mismo se muestran algunas soluciones dadas al problema para un puente gemelo.
Refuerzo de elementos estructurales mediante bandas de acero adheridas con resinas epóxicas
Cifuentes Cifuentes Gustavo
Ingeniería e Investigación , 1995,
Abstract: This paper presents the results of test carried out on reinforced concrete beams strengthened by epoxy-bonded steel plates in the Universidad Nacional de Colombia. The test shown the very good behavior of reinforced beams whit additional bonded plates when they have additional anchor plates that can overcome the problem of anchorage failure. When the reinforced beam does not have additional anchor plates, the beam present greater values of stiffness, but they have a non-ductile behavior. EI presente artículo muestra los resultados experimentales y las conclusiones de una investigación llevada a cabo en la Universidad Nacional de Colombia, acerca del comportamiento de vigas de concreto reforzado, con refuerzo adicional consistente en laminas de acero adheridas, mediante resinas epóxicas. Los resultados experimentales, demuestran el buen comportamiento que tiene este tipo de refuerzo siempre y cuando se disponga de un sistema adicional de anclaje de las láminas de acero. Aunque no se disponga de anclaje adicional se comprobó un aumento de rigidez en las vigas estudiadas.
Marcas discursivas y narrativas del humor leve en Papelucho
Literatura y lingüística , 2011, DOI: 10.4067/S0716-58112011000200002
Abstract: in this paper we analyze the humorous perspective of the events contained in the children's novel papelucho, writen by marcela paz. the methodology is based mainly on the essay of italo calvino "lightness". the analysis consists of detecting textual cues that evidence the predominancy of unusual consciousness and knowledge types while observing the referred facts; also, other cues such as word reference dissolution and the appearance of figurative images of lightness are considered. the results would lead us to make interpretative assumptions about the particularities of humor and world vision, from both the character and the humoristic consciousness that articulate the story.
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