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Search Results: 1 - 10 of 76574 matches for " Salazar Schettino Paz Maria "
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Analysis of Variability of Clones and Subclones of Trypanosoma cruzi Derived from Mexican Strains by the Behavior in Mice and Culture Cells
Marco Antonio Becerril Flores,Salazar Schettino Paz Maria,Ramirez Zamudio Lina
Research Journal of Medical Sciences , 2012,
Abstract: To better understand the biological heterogeneity of behavior of Trypanosoma cruzi strains isolated from Mexico, 6 strains of T. cruzi were cloned by the Miles method (drops of suspension with parasites were diluted in phosphate-buffered saline solution and inoculated to mice).Virulence and infectivity of 10 or 11 clones derived from each strain were determined in female Balb/c mice and Vero culture cells, respectively. Variability of clones was determined by Tukey F statistic test. Only 1 strain, T5, showed interclonal variability and its clones were subcloned by the same method; they showed similar behavior as their parental clones. Clones increased the virulence or had the same behavior after maintaining them for 1 year in mice. Seven clones were eliminated and the virulence of the 4 remaining clones was attenuated when they were maintained in LIT axenic culture for 1 year. The study showed that the strains of T. cruzi in Mexico are either monoclonal or polyclonal and the clones could be eliminated or selected from the environment along time; therefore it is possible to observe that the behavior of T. cruzi strains can change when they are maintained in laboratory for several years.
Evaluación de la función y sincronía de la contracción ventricular en pacientes con enfermedad de Chagas en estadio de latencia
Nivardo Sobrino, Ayax;Jiménez-ángeles, Luis;Bialostozky, David;Vázquez, Clara;Martínez, Irma;Salazar-Schettino, Paz Maria;Bucio-Torres, Martha;Ruiz-Hernández, Adela;Cabrera-Bravo, Margarita;
Archivos de cardiología de México , 2009,
Abstract: objective: to compare the left ventricular function and the ventricular synchrony in patients with chagas disease in latency stage respect to a control group. methods: we analyze a prospective, comparative, transversal and non randomized study of the left ventricular function (lvf) and the ventricular contraction synchronicity (vcs) in 36 subjects with positive serology for chagas disease (18 males and 18 females), with mean of 15 ± 5 years old. the findings were compared with respect to 23 control volunteers (11 males and 12 females) with mean of 28 ± 5 years old. lvf and vcs were evaluated using equilibrium radionuclide angiography images (erna). the comparison of both chagas and control populations was carried out by t student test for independent samples, considering a statistically significant value of p < 0.05. results: the parameters of the ventricular function and the ventricular synchronicity in subjects with positive serology for chagas disease were not statistically different with respect to the parameters of the control group. however, although they have a homogeneous contraction, the mean time of contraction for the right and the left ventricle is statistically smaller with respect to the control group. conclusions: in clinically incipient stages of chagas disease we do not found abnormalities in the ventricular function and the ventricular synchronicity. it's necessary to consider the follow up of the studied populations using indices for the identification of abnormalities of the autonomic nervous system.
Phylogeographic Pattern and Extensive Mitochondrial DNA Divergence Disclose a Species Complex within the Chagas Disease Vector Triatoma dimidiata
Fernando A. Monteiro, Tatiana Peretolchina, Cristiano Lazoski, Kecia Harris, Ellen M. Dotson, Fernando Abad-Franch, Elsa Tamayo, Pamela M. Pennington, Carlota Monroy, Celia Cordon-Rosales, Paz Maria Salazar-Schettino, Andrés Gómez-Palacio, Mario J. Grijalva, Charles B. Beard, Paula L. Marcet
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0070974
Abstract: Background Triatoma dimidiata is among the main vectors of Chagas disease in Latin America. However, and despite important advances, there is no consensus about the taxonomic status of phenotypically divergent T. dimidiata populations, which in most recent papers are regarded as subspecies. Methodology and Findings A total of 126 cyt b sequences (621 bp long) were produced for specimens from across the species range. Forty-seven selected specimens representing the main cyt b clades observed (after a preliminary phylogenetic analysis) were also sequenced for an ND4 fragment (554 bp long) and concatenated with their respective cyt b sequences to produce a combined data set totalling 1175 bp/individual. Bayesian and Maximum-Likelihood phylogenetic analyses of both data sets (cyt b, and cyt b+ND4) disclosed four strongly divergent (all pairwise Kimura 2-parameter distances >0.08), monophyletic groups: Group I occurs from Southern Mexico through Central America into Colombia, with Ecuadorian specimens resembling Nicaraguan material; Group II includes samples from Western-Southwestern Mexico; Group III comprises specimens from the Yucatán peninsula; and Group IV consists of sylvatic samples from Belize. The closely-related, yet formally recognized species T. hegneri from the island of Cozumel falls within the divergence range of the T. dimidiata populations studied. Conclusions We propose that Groups I–IV, as well as T. hegneri, should be regarded as separate species. In the Petén of Guatemala, representatives of Groups I, II, and III occur in sympatry; the absence of haplotypes with intermediate genetic distances, as shown by multimodal mismatch distribution plots, clearly indicates that reproductive barriers actively promote within-group cohesion. Some sylvatic specimens from Belize belong to a different species – likely the basal lineage of the T. dimidiata complex, originated ~8.25 Mya. The evidence presented here strongly supports the proposition that T. dimidiata is a complex of five cryptic species (Groups I–IV plus T. hegneri) that play different roles as vectors of Chagas disease in the region.
Tres especies de triatominos y su importancia como vectores de Trypanosoma Cruzi en México
Salazar Schettino,Paz M.; de Haro Arteaga,Irene; Cabrera Bravo,Margarita;
Medicina (Buenos Aires) , 2005,
Abstract: observations made in the field and in the laboratory on three vectors of trypanosoma cruzi: triatoma barberi, t. pallidipennis and t. dimidiata are here presented. results are compared in relation with their ecotopes, vectorial capacity, biological characteristic, entomological indexes and capture places. in relation with their ecotopos t. barberi has preference for the interior of the human home, in walls, directly in contact with beds; t. pallidipennis, when it is inside the houses, prefers floors and among clothes, although their main ecotope is wild; t. dimidiata has localization in floors, specially under the beds, in the angle between wall and floor; t. barberi and t. dimidiata are attracted by the light. the cycles of life were of 523, 171 and 510 days, respectively for triatoma barberi, t. pallidipennis and t. dimidiata. the vectorial capacity was determined according to the time taken in defecating during or after its sucking blood: t. barberi defecates during its feeding, while t. pallidipennis and t. dimidiata make it from 10 to 20 and of 20 to 30 minutes after starting the process, respectively. the indexes of natural infection were of 56.6 for t.barberi, 29 for t.pallidipennis and 15.6 for t. dimidiata, the metacyclogenics indexes that indicate percentages of metacyclics trypomastigotes in later intestine of vectors was in t. barberi 76.6, t. pallidipennis 15 and t. dimidiata 26, with what it can be concluded that the first species is the best transmitter of t. cruzi in mexico.
Cuticular hydrocarbons of Chagas disease vectors in Mexico
Juárez, M Patricia;Carlson, David A;Salazar Schettino, Paz María;Mijailovsky, Sergio;Rojas, Gloria;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000600012
Abstract: capillary gas-liquid chromatography was used to analyse the cuticular hydrocarbons of three triatomine species, triatoma dimidiata, t. barberi and dipetalogaster maxima, domestic vectors of chagas disease in mexico. mixtures of saturated hydrocarbons of straight and methyl-branched chains were characteristic of the three species, but quantitatively different. major methylbranched components mostly corresponded to different saturated isomers of monomethyl, dimethyl and trimethyl branched hydrocarbons ranging from 29 to 39 carbon backbones. sex-dependant, quantitative differences in certain hydrocarbons were apparent in t. dimidiata.
Comportamiento biológico de Triatoma pallidipennis (Hemiptera: Reduviidae) en el estado de Morelos, México
Bautista,Norma; Rojas,Gloria; De Haro,Irene; Bucio,Martha; Salazar Schettino,Paz María;
Boletín chileno de parasitología , 2001, DOI: 10.4067/S0365-94022001000200004
Abstract: t. pallidipennis was found naturally infected in localities of domestic, peridomestic and wild areas in the state of morelos, méxico. in agreement with the triatomines high potentiality like vectors of t. cruzi, protozoan that causes chagas' disease and the knowledge of these bugs in méxico, the aim of the present research was to collect triatomines for assessment of t. cruzi infection, to characterize and compare life cycles, identification of feeding preferences and observe indexes of experimental infectivity. seven isolates of t. cruzi were obtained, from differents localities which curves of parasites showed different behaviors. histopathological study, carried out by counting of amastigotes nests, which affected the gastrocnemius muscle and heard was observed. the life cycles of t. pallidipennis was completed in 131 days. the feeding preferences were rats, cats and pigs.
Iniciativa México: Propuesta para el control y vigilancia epidemiológica de la enfermedad de Chagas en México
Salazar Schettino,Paz María; Cravioto Q,Alejandro; Tapia Conver,Roberto;
Boletín chileno de parasitología , 2001, DOI: 10.4067/S0365-94022001000200008
Abstract: it is showed the programme for the knowledge, control and epidemiological surveillance of chagas disease in mexico.
Distribución Espacial de Vectores de la Enfermedad de Chagas en el Estado de Guanajuato 1998-2000 Distribución Espacial de Vectores de la Enfermedad de Chagas en el Estado de Guanajuato 1998-2000
Jorge López Cárdenas,Francisco Ernesto González Bravo,Paz María Salazar Schettino
Acta Universitaria , 2012,
Abstract: This study has been perfomed in order to verify the existence and location of vectors of Chagas disease in the entities of Guanajuato state to limit the territories and risk areas. Determining the infection of Trypanosoma cruzi in the vectors, evaluates the risk to human population. This research has been conducted evaluating the information of the labeled triatominos obtained by the field personnel of the Secretaria de Salud del estado de Guanajuato, and classified by the Laboratorio Estatal de Salud del Estado de Guanajuato. The information was obtained from 1998 to 2000 in the entities where work brigades are located and the reported abscence of vectors probably is due the absence of search on them. The index of infestation was not performed due the different search intensity across the territory and the sample method. Just the location of every studied specie to the collecting area was established. Triatoma mexicana, T. barberi, T. longipennis, T. pallidipennis y T. dimidiata, was present in 162 places of 25 entities of the states; at heighs of 870 to 2200 meters above sea level. Inside the house is the site where T. mexicana was present in a major frecuency, for T. barberi was the yard, for T. longipennis inside the house, as well as the T. pallidipennis and the ninphs, T. dimidiata which was collected once, did not reported collect site. El presente estudio se realizó con la finalidad de llevar a cabo la verificación de la existencia y la ubicación de los vectores de la enfermedad de Chagas en los municipios del Estado de Guanajuato y a través de esto, delimitar áreas de distribución espacial de las especies dentro de este territorio, contribuyendo así, a establecer zonas de riesgo y al determinar la infección del Trypanosoma cruzi en los vectores, se conocerá el riesgo para la población humana. Esta investigación se realizó a través de la información del envío etiquetado de triatominos obtenidos por personal de campo de la Secretaría de Salud de Guanajuato y enviados para su clasificación taxonómica al Laboratorio Estatal de Salud Pública del mismo estado. Se recabó información de tres a os (1998 - 2000) de los municipios donde se encuentran brigadas de trabajo y la ausencia reportada de vectores en algunos de ellos, probablemente se debe a la falta de búsqueda en los mismos. No se realizó el índice de infestación de vectores debido a que el método de búsqueda no tuvo la misma intensidad en todo el territorio, ni el método de muestreo lo permitió; sólo se estableció la localización de cada una de las especies según el área de donde fue colectada.
Cuticular hydrocarbons of Chagas disease vectors in Mexico
Juárez M Patricia,Carlson David A,Salazar Schettino Paz María,Mijailovsky Sergio
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: Capillary gas-liquid chromatography was used to analyse the cuticular hydrocarbons of three triatomine species, Triatoma dimidiata, T. barberi and Dipetalogaster maxima, domestic vectors of Chagas disease in Mexico. Mixtures of saturated hydrocarbons of straight and methyl-branched chains were characteristic of the three species, but quantitatively different. Major methylbranched components mostly corresponded to different saturated isomers of monomethyl, dimethyl and trimethyl branched hydrocarbons ranging from 29 to 39 carbon backbones. Sex-dependant, quantitative differences in certain hydrocarbons were apparent in T. dimidiata.
First Case of Natural Infection in Pigs: Review of Trypanosoma cruzi Reservoirs in Mexico
Salazar-Schettino Paz María,Bucio Martha Irene,Cabrera Margarita,Bautista Jacobo
Memórias do Instituto Oswaldo Cruz , 1997,
Abstract: An epidemiological research project was performed in the State of Morelos including collection of samples for blood smears and culture, serological tests, and xenodiagnoses from a total of 76 domestic and peridomestic mammals. Two strains of Trypanosoma cruzi were isolated by haemocultures; one from a pig (Sus scrofa), the first case of natural infection reported in Mexico, and the other from a dog (Canis familiaris). This study summarizes current information in Mexico concerning confirmed reservoirs of T. cruzi
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