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Semi-Quantitative Analysis of Endometrial HOXA10 and BTEB1 Mrna Expressions in the Implantation Window of Patients With Endometriosis and Myoma
Shokrzadeh Sh,Saidijam M,Dehghan A,Esna-Ashari F
Tehran University Medical Journal , 2012,
Abstract: Background: The techniques used in assisted reproductive technologies have progressed considerably, but many embryos do not implant after transfer upon the use of these techniques. One of the causes of infertility is repeated implantation failure due to decreased endometrial receptivity. Furthermore, in clinical conditions such as endometriosis and myoma, implantation decreases after embryo transfer. In this case-control study the expression patterns of HOXA-10 and BTEB1 mRNAs were evaluated at the time of implantation in patients with myoma and endometriosis.Methods : In this study performed in Hamadan University of Medical Sciences during 1389, the cases included 16 patients with endometriosis and myoma (8 in each group) and the control group consisted of 8 fertile women. Endometrial sampling was performed at mid-secretory phase. Later, the expression patterns of HOXA-10 and BTEB1 mRNAs were evaluated using a semi-quantitative RT-PCR method. Results : The optimal PCR cycles determined were 30, 32 and 26 for HOXA10, BTEB1 and β-actin, respectively. Endometrial HOXA-10 and BTEB1 mRNA expression levels (normalized to -actin expression) at the time of implantation were significantly decreased in the endometrium of infertile patients with endometriosis compared with that of healthy fertile controls (P<0.05). A similar pattern was seen in patients with myomas for both HOXA10 and BTEB1 genes, (P<0.05). Conclusion: It seems that lower expression of HOXA-10 and BTEB1 mRNAs in the implantation window of endometrium that increase normally, could account for some aspects of infertility in patients with endometriosis and myoma.
Designing and constructing an 100 bp DNA Ladder by combining PCR and enzyme digestion methods
Saidijam M,Khanahmad Shahreza H,Rikhtegaran Tehrani Z,Karimizare S
Tehran University Medical Journal , 2011,
Abstract: "n Normal 0 false false false EN-US X-NONE AR-SA MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Arial; mso-bidi-theme-font:minor-bidi;} Background: Molecular DNA markers are one of the most important tools in molecular biology labs. The size of DNA molecules is determined by comparing them with known bands of markers during gel electrophoresis. There are many different protocols to produce these kinds of molecular markers. In this study we have suggested an efficient strategy to produce molecular weight markers in industrial proportions."n"nMethods : To achieve the desired sizes of DNA fragments, a combination of two previously known methods, restriction enzyme digestion and polymerase chain reaction (PCR), were used. The enzymatic digestion process was based on designing and constructing plasmids which equaled in size with the desired length of DNA fragments and produced the desired DNA fragment upon linearization. In the PCR method, the desired length of DNA fragments were cloned in multiple cloning sites of pTZ57R plasmid and in a PCR reaction, the new constructed plasmid was used as a template to produce the final fragment."n"nResults : Upon application of this strategy, 2000 and 3000 bp DNA fragments were produced by enzymatic digestion of plasmids of the same size. Moreover, 100 to 1500 bp fragments were produced during PCR using only a set of forward and reverse primers at the flanking region of pTZ57R multiple cloning site."n"nConclusion: The highest advantage of this cost-benefit approach is to produce different types of molecular weight markers by using an effective and short protocol."n"nKeywords: DNA markers, DNA Ladder, agarose gel electrophoresis, molecular weight.
Association Between Cholestryl Ester Transfer Protein D442G Polymorphism on Serum Lipid Levels and CETP Activity in Hypercholesterolemic Patients
Barkhordari A,Hassanzadeh T,Saidijam M,Esmaeili R
Tehran University Medical Journal , 2012,
Abstract: Background: Hypercholesterolemia is considered a major risk factor for pancreatitis, atherosclerosis and coronary heart disease. Cholesteryl ester transfer protein gene polymorphisms are known to be associated with changes in lipid levels. We investigated the association between a polymorphism in the CETP gene (D442G) with plasma lipid levels and CETP activity in patients with hypercholesterolemia. Methods: This case/control study that be done in Hamadan university of medical sciences (from October 2008 to September 2009), included 102 patients with hypercholesterolemia and 200 healthy individuals. Polymerase chain reaction and restriction fragment length polymorphisms were used to determine genotypic distribution and allelic frequencies of polymorphisms. The plasma CETP activity was measured by a kit in a fluorescence spectrometer. Lipid concentrations were measured by routine biochemical and enzymatic assays. Results: Plasma cholesteryl ester transfer protein activity was significantly higher in the cases than the controls (P<0.05). The genotypic and allelic frequencies for this polymorphism were not statistically different between the patients with hypercholesterolemia and the controls (in controls: DD 96%, DG 4%, GG 0% and in cases: DD 86%, DG 10%, GG 4%), (P>0.05). Plasma HDL-C, LDL-C and TC were higher in both groups with GG and DG genotypes than with DD genotype, whereas serum CETP activity was lower in GG genotype compared with other genotypes (GD or DD), (P<0.05). Conclusion: The results showed that D442G polymorphism of CETP gene was associated with changes in lipid profile and plasma CETP activity in the selected population and it might have a role in contributing to a genetic risk for developing coronary artery disease.
Pediculus Capitis Infestation According to Sex and Social Factors in Hamedan-Iran
Mansour Nazari,Massoud Saidijam
Pakistan Journal of Biological Sciences , 2007,
Abstract: To determine the intensity of Pediculus capitis infestation (abundance) among school children, children`s sex and social factors were analyzed as modifiers of the general prevalence of parasitism. The study included 847 school children (407 girls, 440 boys) between 6 and 12 years, from 12 in public rural primary schools of Hamedan, Province of Hamedan, Iran. Classic prevalence was obtained as the percentage of children with nits and/or lice. The general prevalence was 6.85% (girls: 13.5%; boys: 0.7%, p< 0.001), head lice were much more commonly detected in girls than in boys. The obtained results showed that there was significant variations between head lice infestation and the factors such as parents` literacy, type of hair, previous infestation, sharing of bed and comb and care centers, while there was no significant variation between school grade, parents` job, members of family and pediculosis in the studied areas (p> 0.05). Sex and social factor are important modifiers of P. capitis general prevalence and degree of infestation. The classification of children by intensity of infestation allowed a more precise delimitation of this condition, which is especially important for disease surveillance and application of control measures.
BC4707 Is a Major Facilitator Superfamily Multidrug Resistance Transport Protein from Bacillus cereus Implicated in Fluoroquinolone Tolerance
Roger Simm, Aniko V?r?s, Jaakko V. Ekman, Marianne S?dring, Ingerid Nes, Jasmin K. Kroeger, Massoud Saidijam, Kim E. Bettaney, Peter J. F. Henderson, Mirja Salkinoja-Salonen, Anne-Brit Kolst?
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036720
Abstract: Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more specific, but modest role in the efflux of norfloxacin. In addition to this, transcriptional analyses showed that BC4707 is also expressed during growth of B. cereus under non-stressful conditions where it may have a role in the normal physiology of the bacteria. Altogether, the results indicate that bc4707, which is part of the core genome of the B. cereus group of bacteria, encodes a multidrug resistance efflux protein that is likely involved in maintaining intracellular homeostasis during growth of the bacteria.
Study of Duct Characteristics Deduced from Low Latitude Ground Observations of Day-Time Whistler at Jammu  [PDF]
M. Altaf, M. M. Ahmad
International Journal of Astronomy and Astrophysics (IJAA) , 2013, DOI: 10.4236/ijaa.2013.33032
Abstract:

Propagation characteristics of low latitude whistler duct characteristics have been investigated based on day-time measurements at Jammu. The morphogical characteristics of low latitude whistlers are discussed and compared with characteristics of middle and high latitude whistlers. The Max. electron density (Nm) at the height of the ionosphere obtained from whistler dispersion comes out to be higher than that of the background which is in accordance with the characteristics of whistler duct. The equivalent width is found to be close to the satellite observations and the characteristics of whistler duct in low latitude ionosphere are similar to those in middle and high latitude ionosphere. The width of ducts estimated from the diffuseness of the whistler track observed during magnetic storm is found to lie in the range of 50 - 200 Km.

Review Article: Immobilized Molecules Using Biomaterials and Nanobiotechnology  [PDF]
Magdy M. M. Elnashar
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2010, DOI: 10.4236/jbnb.2010.11008
Abstract: Immobilized molecules using biomaterials and nanobiotechnology is a very interesting topic that touching almost all aspects of our life. It uses the sciences of biology, chemistry, physics, materials engineering and computer science to develop instruments and products that are at the cutting edge of some of today’s most promising scientific frontiers. In this review article, the author based on his experience in this arena has tried to focus on some of the supports for im-mobilization; the most important molecules to be immobilized such as DNA, cells, enzymes, metals, polysaccharides, etc and their applications in medicine, food, drug, water treatment, energy and even in aerospace. He specified a special section on what is new in the arena of supports and technologies used in enzyme immobilization and finally a recommendation by the author for future work with a special attention to up-to-date references.
Using of the generalized special relativity (GSR) in estimating the neutrino masses to explain the conversion of electron neutrinos  [PDF]
M. H. M. Hilo
Natural Science (NS) , 2011, DOI: 10.4236/ns.2011.34044
Abstract: In this work the Generalized Special Relativity (GSR) is utilized to estimate masses of some elementary particles such as, neutrinos. These results are found to be in conformity with experimental and theoretical data. The results obtained may explain some physical phenomena, such as, conversion of neutrinos from type to type when solar neutrino reaches the Earth.
Postischemic Lower Extremity Wounds Treated with and without Negative Pressure Dressing  [PDF]
Naz?mmü?
Surgical Science (SS) , 2012, DOI: 10.4236/ss.2012.37075
Abstract: Background: Vacuum-assisted dressing is a noninvasive closure system of the wound, which makes localized and controlled negative pressure. Its mechanical tension reduces edema, stimulates granulation tissue formation and angiogenesis, and prepares the wound bed for closure. In this study, a patient has been presented, who suffered from serious lower extremity wounds due to arterial emboli, one of her wounds has been treated with vacuum-assisted dressing and the other with conventional dressing to evaluate the efficacy of vacuum-assisted dressing in acute ischemic wounds. Methods: A 65-year-old female patient was admitted to the emergency clinic, due to complaint of severe pain in her right lower extremity which suggested an acute arterial occlusion. She immediately underwent an embolectomy operation; however, a few days later, severe ischemia on the leg and foot became appearant. All of the necrosis was sharply debrided under sterile conditions in the operating room, and then lower leg wound was covered with vacuum-assisted dressing, also calcaneal wound was dressed with silver sulphadiazine. Results: Eighteen days after the first dressing with negative pressure, distal leg wound became ready for closure, whereas, enough granulation tissue over the calcaneal area developed merely thirty-four days later. Calcaneal wound closed spontaneously within fifty-three days. Conclusions: When dealing with this experience, using the negative pressure dressing in patient with severe lower limb wounds following arterial emboli, accelerates wound healing by means of developing the granulation tissue, and rapidly prepares the wound for closure, so it may reduce the risk of amputation.
Laplace Transform Method for Unsteady Thin Film Flow of a Second Grade Fluid through a Porous Medium  [PDF]
M. Ali, M. Awais
Journal of Modern Physics (JMP) , 2014, DOI: 10.4236/jmp.2014.53017
Abstract:

In this article, we have effectively used the Numerical Inversion of Laplace transform to study the time-dependent thin film flow of a second grade fluid flowing down an inclined plane through a porous medium. The solution to the governing equation is obtained by using the standard Laplace transform. However, to transform the obtained solutions from Laplace space back to the original space, we have used the Numerical Inversion of Laplace transform. Graphical results have been presented to show the effects of different parameters involved and to show how the fluid flow evolves with time.

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