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Search Results: 1 - 10 of 97 matches for " Safar Farajnia "
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Screening of Pseudomonas sp. for Cephalosporin Acylase Activity
Asghar Tanomand,Rahib Abeshov,Safar Farajnia
Research Journal of Biological Sciences , 2012,
Abstract: Medically useful semisynthetic cephalosporin antibiotics are made from precursor 7-aminocepha-losporanic acid (7-ACA). Cephalosporin Acylase (CA), which catalyzes hydrolysis of both glutary l-7-aminocephalosporanic acid (GL-7ACA) and cephalosporin C (CPC) to 7-ACA, is thus a very important enzyme for producing semisynthetic beta-lactam antibiotics. The cephalosporin acylase can be found in several Pseudomonas sp. (such as P. putida, P. cepacia BY 21 P. nitroreducens, P. syringae, p. SE83, p. V22, p. SY-77, p. sp.130 , ) and other bacteria. Therefore, screening of cephalosporin asylase positive pseudomonas is very important. We isolated 130 Pseudomonas sp. from clinical and environmental specimens (75 clinical samples, 40 hospital environment samples, 10 water samples, 5 soil samples). All of isolated Pseudomonas sp. were tested for cephalosporin acylase by P.C.R method and analysis of gel electrophoresis patterns. We found only 2 cephalosporin acylase pseudomonas positive from clinical samples. These strains were selected for sequencing and cloning in E. coli and assessment of gene expression and cephalosporin acylase production in others stages of our study.
Dose-Dependent Effect of Flouxetine on 6-OHDA-Induced Catalepsy in Male Rats: A Possible Involvement of 5-HT1A Receptors
Hamdolah Sharifi,Alireza Mohajjel Nayebi,Safar Farajnia
Advanced Pharmaceutical Bulletin , 2013, DOI: 10.5681/apb.2013.033
Abstract: Purpose: Progressive loss of dopaminergic neurons of the substantia nigra pars compacta (SNc) in Parkinson’s disease (PD) leads to impairment of motor skills. Several evidences show that the role of serotonergic system in regulation of normal movement is pivotal and mediates via 5-HT1A receptors. Our previous study has shown that fluoxetine in acute injections able to attenuate catalepsy in 6-hydroxydopamine (6-OHDA)-lesioned rats. Since drugs are used chronically in clinic, in this study we attempted to evaluate effect of chronic administration of fluoxetine on 6-OHDA-induced catalepsy. Methods: Catalepsy was induced by unilateral infusion of 6-OHDA (8 μg/2 μl/rat) into the central region of SNc and assayed by using bar-test. Fluoxetine (1, 2.5, 5 and 10 mg/kg) was injected intraperitonealy (ip) for 10 days and its anti-cataleptic effect was assessed at the 10th day. Results: Fluoxetine in high doses (5 and 10 mg/kg) worsened 6-OHDA-induced catalepsy while it had anti-cataleptic effect at the dose of 1mg/kg. The anti-cataleptic effect of fluoxetine (1mg/kg) was reversed by co-administration with NAN-190 (0.5 mg/kg, ip), as a5-HT1Areceptor antagonist. Conclusion: According to the results it can be concluded that fluoxetine has anti-cataleptic effect in parkinsonian rats only at low doses, whereas at higher doses it worsens catalepsy. It’s anti-cataleptic effect is exerted through affecting on 5-HT1Areceptors. However, at high doses other mechanisms may be involved. Further clinical studies are needed to prove it’s possible clinical application as an adjuvant therapy in reducing catalepsy of PD.
The Effect of Chronic Administration of Buspirone on 6-Hydroxydopamine-Induced Catalepsy in Rats
Hamdolah Sharifi,Alireza Mohajjel Nayebi,Safar Farajnia
Advanced Pharmaceutical Bulletin , 2012,
Abstract: Purpose: Several evidences show that serotonergic neurons play a role in the regulation of movements executed by the basal ganglia. Recently we have reported that single dose of buspirone improved 6-hydroxydopamine (6-OHDA) and haloperidol-induced catalepsy. This study is aimed to investigate effect of chronic intraperitoneal (i.p.) administration of buspirone on 6-OHDA-induced catalepsy in male Wistar rats. Methods: Catalepsy was induced by unilateral infusion of 6-OHDA (8 μg/2 μl/rat) into the central region of the SNc and was assayed by the bar-test method 5, 60, 120 and 180 min after drugs administration in 10th day. The effect of buspirone (0.5, 1 and 2 mg/kg, i.p. for 10 days) was assessed in 6-OHDA-lesioned rats. Results: The results showed that chronic injection of buspirone (0.5, 1 and 2 mg/kg, i.p. for 10 days) decreased catalepsy when compared with the control group. The best anticataleptic effect was observed at the dose of 1 mg/kg. The catalepsy-improving effect of buspirone was reversed by 1-(2-methoxyphenyl)- 4-[4-(2-phthalimido) butyl]piperazine hydrobromide (NAN-190), 0.5 mg/kg, i.p.,as a 5-HT1A receptor antagonist. Conclusion: Our study indicates that chronic administration of buspirone improves catalepsy in a 6-OHDA-induced animal model of parkinson's disease (PD). We also suggest that buspirone may be used as an adjuvant therapy to increase effectiveness of antiparkinsonian drugs. In order to prove this hypothesis, further clinical studies should be done.
Screening of Pseudomonas sp. for Cephalosporin Acylase Activity
Asghar Tanomand,Rahib Abeshov,Safar Farajnia
Research Journal of Biological Sciences , 2008,
Abstract: Medically useful semisynthetic cephalosporin antibiotics are made from precursor 7-aminocepha-losporanic acid (7-ACA). Cephalosporin Acylase (CA), which catalyzes hydrolysis of both glutary l-7-aminocephalosporanic acid (GL-7ACA) and cephalosporin C (CPC) to 7-ACA, is thus a very important enzyme for producing semisynthetic beta-lactam antibiotics. The cephalosporin acylase can be found in several Pseudomonas sp. (such as P. putida, P. cepacia BY 21 P. nitroreducens, P. syringae, p. SE83, p. V22, p. SY-77, p. sp.130 ,...) and other bacteria. Therefore, screening of cephalosporin asylase positive pseudomonas is very important. We isolated 130 Pseudomonas sp. from clinical and environmental specimens (75 clinical samples, 40 hospital environment samples, 10 water samples, 5 soil samples). All of isolated Pseudomonas sp. were tested for cephalosporin acylase by P.C.R method and analysis of gel electrophoresis patterns. We found only 2 cephalosporin acylase pseudomonas positive from clinical samples. These strains were selected for sequencing and cloning in E. coli and assessment of gene expression and cephalosporin acylase production in others stages of our study.
Screening and Identification of PGA Producing E. coli Isolates by PCR Technique
Kafshnochi Magsoud,Safar Farajnia,Raheb Aboshof,Hanieh Rezaee
Research Journal of Biological Sciences , 2012,
Abstract: The PGA gene of Escherichia coli encodes a penicillin G acylase (PGA). Penicillin G acylase 1(PGA, EC 3.5.1.11) is a type II penicillin acylase that hydrolyzes Penicillin G to 6-aminopenicillanic acid (6-APA) and phenyl acetic acid (PAA). PGAs have been found in numerous bacteria and fungi and the PGA of Escherichia coli has been well characterized. In free-living E. coli, PGA is thought to act as a Scavenger enzyme for many different natural esters and amides of PAA and its derivatives, such as hydroxyphenylacetic acid (HPA). In this study E. coli, a member of Enterobacteriasea was investigated for PGA. The main aim of this study was screening of E. coli strains from environmental and clinical specimens for PGA by PCR and then cloning, sequencing and recombinant expression of cloned PGA in the E. coli. Total 280 E. coli isolates were collected from water, soil and clinical specimens. Specimens were transported to the laboratory and then routine tests were done for identification. In this study we found PGA gene in only 6 E. coli isolates. All of PGA positive isolates were from Clinical specimens. The PGA gene from one of the positive isolate were amplified and cloned in pGEM T-easy vector. This clone will be used for further study and production of recombinant Penicillin acylase.
Evaluation of Selective and Nonselective Media for Isolation of Helicobacter pylori from Gastric Biopsy Specimens
M.Y. Alikhani,N. Sadeghifard,Safar Farajnia,M. Hajia
Pakistan Journal of Biological Sciences , 2007,
Abstract: The aim of the present study was to compare six media, three selective and three nonselective media, to determine the best combination of media for the primary isolation of Helicobacter pylori. Over a period of 8 months, mucosal antral biopsy specimens were obtained from 97 dyspeptic patients undergoing endoscopy. Biopsy samples were plated in parallel on all six media. Egg yolk emulsion agar (EYE), Skirrow’ s medium and modified Thayer-Martin medium were used as selective media; modified chocolate agar (MCHOC), Triptycase Soy Agar (TSA) and brain heart infusion agar were used as nonselective media. Overall, by using these six media, H. pylori were recovered from biopsy specimens from 48 of 97 patients, yielding an isolation rate of 49%. Comparison of all possible combinations of the six media showed that the highest rate of isolation of H. pylori was 100% (48 of 48) with EYE-MCHOC, followed by 97% (47 of 48) when EYE-SK was used. Conversely, it was found that none of the media used alone yielded a 100% rate of recovery (the maximum recovery rate was 92%, which was achieved with EYE). These results indicate that the association of EYE and MCHOC yielded the maximum recovery of H. pylori from gastric biopsy specimens. Therefore, the use of selective and nonselective media in parallel offers optimal recovery rates with only a slight increase in costs.
Detection of Acute Childhood Meningitis by PCR, Culture and Agglutination Tests in Tabriz, Iran
Reza Ghotaslou,Safar Farajnia,Fatemeh Yeganeh,Shahram Abdoli-Oskouei
Acta Medica Iranica , 2012,
Abstract: Meningitis is one of the hazardous and life threatening infections and is associated with mortality and morbidity. The aim of this study was to determine etiological agents of childhood bacterial meningitis. The culture, Gram staining, agglutination and PCR assays were used to examine CSF specimens from 277 patients with presumed bacterial meningitis for the occurrence of 4 most common infectious agents consist of N. meningitis, H. influnsae, S. pneumoniae and S. agalactiae between 2008 and 2009 at different wards of the Children Hospital of Tabriz. The mean age of patients was 35±2 (Mean±SEM) month, (minimum 11 days maximum14 years), of all cases 59.6% male and 40.4% female. Overall the diagnosis was confirmed with a CSF culture in 11/277 (3.97%), by agglutination test in 14/277 (5.05%). The isolated bacteria included S. pneumoniae 5 cases, H. influnsae 2 cases, N. meningitis 3 cases and P. aeroginusae 1 case. A positive PCR assay allowed us to diagnose bacterial meningitis in 19 patients (6.8%). In the present study, we found PCR to be a useful and sensitive method for the detection of bacterial DNA in the CSF samples from suspected meningitis patients. Furthermore, to maximize management of meningitis cases, a combination of culture and PCR is necessary.
Screening and Identification of PGA Producing E. coli Isolates by PCR Technique
Kafshnochi Magsoud,Safar Farajnia,Raheb Aboshof,Hanieh Rezaee
Research Journal of Biological Sciences , 2008,
Abstract: The PGA gene of Escherichia coli encodes a penicillin G acylase (PGA). Penicillin G acylase 1(PGA, EC 3.5.1.11) is a type II penicillin acylase that hydrolyzes Penicillin G to 6-aminopenicillanic acid (6-APA) and phenyl acetic acid (PAA). PGAs have been found in numerous bacteria and fungi and the PGA of Escherichia coli has been well characterized. In free-living E. coli, PGA is thought to act as a Scavenger enzyme for many different natural esters and amides of PAA and its derivatives, such as hydroxyphenylacetic acid (HPA). In this study E. coli, a member of Enterobacteriasea was investigated for PGA. The main aim of this study was screening of E. coli strains from environmental and clinical specimens for PGA by PCR and then cloning, sequencing and recombinant expression of cloned PGA in the E. coli. Total 280 E. coli isolates were collected from water, soil and clinical specimens. Specimens were transported to the laboratory and then routine tests were done for identification. In this study we found PGA gene in only 6 E. coli isolates. All of PGA positive isolates were from Clinical specimens. The PGA gene from one of the positive isolate were amplified and cloned in pGEM T-easy vector. This clone will be used for further study and production of recombinant Penicillin acylase.
DNA-Delivery Methods to Produce Transgenic Plants
Behrooz Darbani,Safar Farajnia,Mahmoud Toorchi,Saeed Zakerbostanabad
Biotechnology , 2008,
Abstract: Since the 1980s, diverse methods for plant transformation have been described including biological, chemical and physical based methods. Transformation is performed to introduce novel traits, study basic biological processes, or produce recombinant proteins of interest. We review Agrobacterium-mediated transformation as well as non-biological based approaches for the production of transgenic plants. This review presents the methods of gene transfer into plants, applications, advantages and disadvantages of each method.
Plant Transformation: Needs and Futurity of the Transgenes
Behrooz Darbani,Safar Farajnia,Shahin Noeparvar,C. Neal Stewart Jr.
Biotechnology , 2008,
Abstract: To produce transgenic plants which have various applications in agricultural and non-agricultural fields, a marker gene is necessary to recover a viable transgenic plant. To express or transcribe of transgenes, utilization of promoters is also unavoidable. Analysis of transgenes includes copy number, insertion site, integration stability, expression and it`s pattern and variability is immensely important in order to develop a successful transgenic event. This review presents the necessities for better recovery of transgenic plants, transcription or expression of transgenes, as well as methods to analyze transgenes.
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