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Search Results: 1 - 10 of 271 matches for " SUTIMAN BAMBANG SUMITRO "
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Identification of DNA Photolyase Gene of a Drought Tolerance Branching Line of Kenaf (Hibiscus cannabinus L.)  [PDF]
Estri Laras Arumingtyas, Nur Basuki, Sutiman Bambang Sumitro, Sudjindro  
American Journal of Plant Sciences (AJPS) , 2012, DOI: 10.4236/ajps.2012.36098
Abstract: DNA photolyase is a photoreactivation enzyme which has a role in the mechanism of DNA repair. The existence of DNA photolyase gene could affect the success of mutation process. SM026H is a drought tolerance branching line of kenaf identified by Research Institute for Tobacco and Fibre Crops, Karangploso, Malang, Indonesia which has a potential as a source for mutation breeding. In this research, cloning and sequencing of the DNA photolyase gene of line SM026H were conducted. The DNA of SM026H leaves was isolated using DNeasy Kit, then was amplified by Polymerase Chain Reaction (PCR) using AC1 as a forward primer, paired with AC3R or AC4R as reverse primers and checked using electrophoresis on a 0.7% agarose gel. The AC1-AC3R primer pair produced a DNA fragment with a size of 750 bp, whereas the AC1-AC4R primer pair produced a DNA fragment of 1000 bp in size. Cloning of the AC1-AC3R and AC1-AC4R fragments was done prior to sequencing. Preparation for cloning was done by running and extracting the PCR products from a 0.7% Low Melting Agarose (LMA), and then ligating them to a pCR21 plasmid using an electroporation method. Two sub-fragments of each AC1-AC3R and AC1-AC4R fragments were identified. They were 600 bp and 500 bp, resulting from the AC1-AC3R fragment, and 1300 bp and 500 bp, resulting from the AC1-AC4R fragment. The sequencing result of those sub-fragments was analyzed using a Basic Local Alignment Search Tool (BLAST) program. It was shown that the sequences have a degree of homology to DNA photolyase sequence of Arabidopsis thaliana A. thaliana, Cucumis sativus, Spinacia oleracea, Stellaria longipes and Oryza sativa. These suggested that the kenaf line SM0026H possesses the DNA photolyase gene. However, further study needs to be done to ascertain that the gene was not the cryptochrome.
Isocitrate dehydrogenase, lactate dehydrogenase and α-glycerophosphate dehydrogenase polymorphysm enzyme of Black Tiger Shrimp (Penaeus monodon Fab.) which is hydrogen sulfide resistant
Biodiversitas , 2006,
Abstract: The hydrogen sulfide is one of compound which very often found in the shrimp pond caused by anaerobic decomposition or as a natural condition of the sea water which have volcano activity. This research was obtaining information of the differences of genetic expression between black tiger shrimp which could resist to H2S and the one which could not survive in this H2S. This research also trying to obtain information the genetic variety which could resist to H2S. The genetic variety of black tiger shrimp which could resist to H2S has been analysis with allozyme electrophoresis technique, using specific tissue meat and buffer CAPM (Citric Acid Aminopolimorpholine) pH 6. From the three enzymes analyzed it could be detected that IDH enzyme (Isocitrate dehydrogenase) has locus polymorphic, whereas enzyme α-GDP (α-glycerophosphate dehydrogenase) and LDH (lactate dehydrogenase) with monomorphic locus. The average heterozygosity for the group which could resist to H2S is 0.070, whereas the group which could not survive in the H2S is 0.042. The control group has heterozygosity 0.041. The group which could resist to H2S with higher heterozygosity will have bigger chance to survive and have better adaptation ability in environmental changes. A high heterozygosity made possible for genetic population improvement by exploiting the good gene.
Isolation and Identification of Transforming Growth Factor β from In Vitro Matured Cumulus Oocyte Complexes
HAYATI Journal of Biosciences , 2012,
Abstract: Transforming growth factor-β (TGF-β) is a two-chain polypeptide with molecular weight of 25 kDa which takes significant role in the steroidogenesis process. In the ovarian oocyte in particular, TGF-β has an important role in regulating reproductive function. TGF-β represents a key intrafollicular protein that regulates follicle development and aromatization process. The purpose of this research was to characterize and identify a protein fraction of TGF-β from the bovine isolated oocytes, which is synthesized during in vitro oocyte maturation process. Oocytes were collected from follicles with diameter of 3-8 mm. Oocytes were then matured in TCM 199 media supplemented with 5 μg/mg LH, 3% BSA, and 50 μg/ml gentamicin sulfate, and cultured in CO2 incubator (5%, 38.5 oC) for 20 hours. TGF-β receptors were identified immunohistochemically. Characteristics of the TGF-β protein were determined using SDS PAGE and TGF-β specification was tested using Western Blotting. The results showed that TGF-β receptors were identified and found in cumulus oocyte complexes (COCs). TGF-β protein was isolated from bovine oocytes with molecular weight 25 kDa and it was identified by Western blotting methods in the same molecular weight.
Basic Knowledge and Reasoning Process in the Art Creation  [PDF]
Bambang Sunarto
Open Journal of Philosophy (OJPP) , 2015, DOI: 10.4236/ojpp.2015.55036
Abstract: Basically, process in the art creation is supported by three pillars of existence, namely 1) activity, 2) method, and 3) knowledge. In the application of activities, methods, and knowledge are always accompanied by the reasoning of the creators of art. Reasoning is used to obtain formulation of practical knowledge, productive knowledge, and theoretical knowledge of an object. The creators in directing its attention to the target of creation use a set of logically interrelated concepts, supported by reasoning in varied models. Substance of reasoning contains of several elements, i.e., confidence, the will to work, models, concepts, methods of concept application and the artwork. The mastery of reasoning and the material elements of reasoning are important issues in the development of pillars of artwork creation.
Use of the Language Laboratory in the Communicative Methodology
Bambang Sugeng
TEFLIN Journal , 1998,
Abstract: Abstract : The language laboratory has almost been forgotten lately partly due to intensive implementation of the communicative meth-odology in language teaching. This is unfortunate considering that, as a medium of instruction, the language laboratory is, like all other media, a means of reaching a goal. In this scheme, use of the language lab remains relevant. In order for the language lab to suit the commu-nicative purposes certain requirements need to be fulfilled. First, since the goal of the communicative methodology is directed to communi-cative competence, the use of the language lab should be directed for this purpose. Second, a design should be attempted where use of the language laboratory will expose students with authentic language usc.Third, instructional strategics should be in concord with the coma€‘ municative principles such as communicative gaps, pre- and post-activities, and othcrs.Finally, lessons should be built integratively. Examples are given illustrating integration of the listening, speaking, reading, and writing skills in lesson units.
Choosing Different Contour Interval on a Fully Raster-Based Erosion Modeling: Case Study at Merawu Watershed, Banjarnegara, Central Java
Bambang Sulistyo
Jurnal Tanah Tropika , 2011,
Abstract: The research was aimed to study the efect of choosing different contour interval to produce Digital Elevation Model on a fully raster-based erosion modeling of The Universal Soil Loss Equation using remote sensing data and a geographical information system technique. Methods were applied by analyzing all factors that affecting erosion in GIS environment such data were in the form of raster. Those data were R , K, LS, C and P factors. LS factor was derived from Digital Elevation Model by taking flow direction from each pixel into consideration. Research used 3 contour intervals to produce Digital Elevation Model, i.e. 12.5, 25 and 50 meter. C factor was derived from the formula after applying linearly regression analysis between Normalized Difference Vegetation index of remote sensing data and C factor measured directly on the field. Another analysis was the creation of map of Bulk Density used to convert erosion unit as from Mg ha-1mo-1 to mm mo-1. To know the model accuracy, validation of the model was done by applying statistical analysis and by comparing the result of erosion model (Emodel) with actual erosion (Eactual) which was measured regularly in Merawu watershed. A threshold value of > 0.80 or > 80% was chosen to justify whether the model was accurate or not. The results showed that all Emodel using 3 countour intervals have correlation value of > 0.8. These results were strenghtened with the result of analysis of variance which showing there were no difference between Emodel and Eactual. Among the 3 models, only Emodel using 50 meter countour interval reached the accuracy of 81.13% while the other only had 50.87% (using countour interval 25 meter) and 32.92% (using countour interval 12.5 meter).
Bambang Robi’in
Jurnal Informatika , 2012,
Abstract: Sistem informasi geografis merupakan teknologi untuk mengelola, menganalisis dan menyebarkan informasi geografis yang berkaitan dengan tata letak keruangan dan informasi-informasi yang terkait. Berkaitan dengan sumber daya alam, maka informasi geografis akan berisi mengenai lokasi suatu daerah, dan informasi mengenai sumber daya alam yang terkandung pada daerah tersebut. Perubahan-perubahan yang terjadi kapan saja pada alam berkaitan dengan penambahan atau pengurangan data akan dapat dilakukan secara uptodate tanpa harus membuat baru. Dalam membangun sistem informasi geografis sumber daya alam indonesia indonesia ini menggunakan mapserver sebagai server pemetaan dan script php sebagai bahasa pemrograman web dalam membangun halaman web. Penelitian dilakukan melalui empat tahap yaitu Persiapan, Perancangan, Implementasi, dan tahap Uji coba. Hasil dari penelitian ini adalah Sistem informasi geografis yang menampilkan peta digital dapat disimpan dalam bentuk shape file yang dapat dibuat menggunakan salah satu perangkat lunak sistem nformasi berbasis desktop yaitu ArcView. Selain itu, Sistem infomasi geografis sumber daya alam dapat dijadikan sebagai salah satu sumber inforasi mengenai sebaran sumber daya alam indonesia yang dapat diakses oleh masyarakat luas baik dari dalam maupun luar negeri.
Jambak Jambu Kalko: Nature conservation management of the Serampas of Jambi, Sumatra
Biodiversitas , 2012,
Abstract: Hariyadi B. 2012. Jambak Jambu Kalko: Nature conservation management of the Serampas of Jambi, Sumatra. Biodiversitas 13: 40-45. Serampas is an indigenous group that still inhabits forested areas around the Kerinci Seblat National Park (KSNP) in the island of Sumatra, Indonesia. The group has occupied the area since several centuries ago, long before the KSNP was officially proclaimed as a national park. The blending of local natural conditions, socio-cultural setting and cosmology has gradually produced a number of local wisdoms and practices to manage the local natural resources. The Serampas traditional resource management practices were not only friendly to the environment, but also encourage the more equitable distribution of natural resources among the members of the Serampas community. Jambak jambu kalko is literally means a list of locally important perennial fruit tree species protected by the Serampas customary system. Beyond the jambak jambu kalko the Serampas also recognize customs, traditions, and values that have important role in managing the local natural resources.
FORUM: The Roots of Violence in Indonesian Political History
Bambang Purwanto
Kasarinlan : Philippine Journal of Third World Studies , 2001,
Abstract: Many scholars do not understand the reality of the violence in Indonesia. They point to some individual or group or to the political structure of the New Order as the dalang (mastermind) for the state-sponsored violence of the Suharto and the previous Sukarno regimes. The fact is, it is no longer a structural problem but a socio-cultural one. It is not only Suharto but also Sukarno and Nasution who are responsible for the tradition of killing people to end all movements against the government. The origin of violence should be found long before the New order or the Soeharto presidency. It is in the whole process of Indonesian history since its independence, where the militaristic nature and overwhelming distrust within the state and society were created. At this stage, clearly I have some differences compared to well-known Indonesianists in understanding the present riots or social violence in Indonesia.
Bambang Winarko
International Journal of Electronic Commerce Studies , 2011,
Abstract: The Indonesian Center for Library and Technology Dissemination (ICALTD) has a duty to disseminate agricultural information to researchers, extension workers, academicians, and other general users. Previously, the dissemination was based on printed publications. However, along with the penetration and development of the internet, the method of dissemination has changed to ITC-based dissemination. CD/VCD and internet technology application plays an important role in ICALTD’s dissemination of agricultural information. The involvement of internet technology in the dissemination of agricultural information ranges from purchasing online electronic journals for the main library collection to electronic-based processing and internet-based services. Efforts to educate stakeholders, such as administrators, content providers, and users, are carried out to ensure the successful dissemination of agricultural information.
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