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Search Results: 1 - 10 of 35 matches for " SUTARNO "
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Sequence variation of bovine mitochondrial ND-5 between haplotypes of composite and Hereford Breeds of beef cattle
SUTARNO
Biodiversitas , 2002,
Abstract: The aims of the study were to: Investigate polymorphisms in the ND-5 region of bovine mitochondrial DNA in the composite and purebred Hereford herds from the Wokalup selection experiment, sequencing and compare the sequences between haplotypes and published sequence from Genebank. A total of 194 Hereford and 235 composite breed cattle from Wokalup Research Station were used in this study. The mitochondrial DNA was extracted using Wizard genomic DNA purification system from Promega. ND-5 fragment of mitochondrial DNA was amplified using PCR and continued with RFLP. Each haplotypes were sequenced. PCR products of each haplotype were cloned into pCR II, transformed, colonies selection, plasmid DNA extraction continued with cycle sequencing. Polymorphisms were found in both breeds of cattle in ND-5 region of mitochondrial DNA by PCR-RFLP analysis. Sequencing analysis confirmed the RFLPs data.
REVIEW: Genetic Diversity: Detection of Gene Variation at the DNA Level and Utilization of Gene Markers on Locating QTLs
SUTARNO
Biodiversitas , 2003,
Abstract: Advanced techniques of molecular biology have provided the opportunity to study genetic diversity within and among breeds at the single gene level. Many DNA markers, either of genomic DNA or cytoplasmic DNA, have been generated recently by utilizing molecular techniques, such as RFLP, microsatellites, PCR-RFLP, RAPD, sequencing etc. PCR-based techniques have recently progressed rapidly for the detection of both known- and unknown-mutation detections that may be applied in locating gene marker for economically important traits. There are basically two different approaches of locating quantitative trait loci (QTLs), candidate gene and random approaches. The first approach is based on prior supporting knowledge of physiological and biochemical evidence, showing that the gene is involved in the trait(s) of interest, while the random marker approach attempts to locate gene markers by measuring genotypes at a large number of loci with unknown phenotypic effects, in the hope that the loci are linked to a QTL influencing the trait of interest.
Genetic variations among Indonesian native cattle breeds based on polymorphisms analysis in the growth hormone loci and mitochondrial DNA
SUTARNO
Biodiversitas , 2010,
Abstract: Sutarno (2010) Genetic variations among Indonesian native cattle breeds based on polymorphisms analysis in the growth hormone loci and mitochondrial DNA. Biodiversitas 11: 1-5. Genetic variation within breeds is important and its study has become a subject of interest in livestock species, as it has many applications in animal breeding and genetics, such as the identification of animals and parentage testing, gene mapping and identifying markers for performance traits. Two loci of bovine growth hormone genes, and two regions of mitochondrial DNA, D-loop and ND-5 were characterized using polymerase chain reaction – restriction fragment length polymorphism (PCR-RFLP) involving 120 Indonesian native cattle of Bali, Madura, PO and West Sumatra breeds. The results indicated that sequence variations were detected both in the growth hormone loci and mitochondrial DNA.
Synthesis of Faujasite from Fly Ash and its Applications for Hydrocracking of Petroleum Distillates
Sutarno Sutarno,Yateman Arryanto
Bulletin of Chemical Reaction Engineering & Catalysis , 2007, DOI: 10.9767/bcrec.2.2-3.9.45-51
Abstract: The synthesis of faujasite from fly ash and its application for hydrocracking catalyst of heavy petroleum distillates have been performed. Faujasite was synthesized from fly ash by hydrothermal reaction in alkaline solution via combination of reflux pretreatment of fly ash with HCl and fusion with NaOH. The preparation of nickel containing catalysts by ion exchange method under similar initial concentration of nickel resulted higher amount of nickel loaded on faujasite than those on zeolite Y, however, the structural damage of faujasite was higher than those of zeolite Y. In the hydrocracking of heavy petroleum distillates over Ni-faujasite and Ni-zeolite Y catalysts, the conversion of heavy gas oil fraction was the most pronounced. The selectivity toward hydrocarbons in the range of gasoline and kerosene obtained over Ni-faujasite catalyst was lower compared to those of Ni-zeolite Y reference catalyst. 2007 BCREC UNDIP. All rights reserved. [Presented at Symposium and Congress of MKICS 2007, 18-19 April 2007, Semarang, Indonesia] [How to Cite: S. Sutarno, Y. Arryanto. (2007). Synthesis of Faujasite from Fly Ash and its Applications for Hydrocracking of Petroleum Distillates. Bulletin of Chemical Reaction Engineering and Catalysis, 2 (2-3): 45-51. doi:10.9767/bcrec.2.2-3.9.45-51] [How to Link/DOI: http://dx.doi.org/10.9767/bcrec.2.2-3.9.45-51 ] [Cited by: Scopus 1 | ScienceAsia |]
Constrained robust estimation of magnetotelluric impedance functions based on a bounded-influence regression M-estimator and the Hilbert transform
D. Sutarno
Nonlinear Processes in Geophysics (NPG) , 2008,
Abstract: Robust impedance estimation procedures are now in standard use in magnetotelluric (MT) measurements and research. These always yield impedance estimates which are better than the conventional least square (LS) estimation because the 'real' MT data almost never satisfy the statistical assumptions of Gaussian distribution upon which normal spectral analysis is based. The robust estimation procedures are commonly based on M-estimators that have the ability to reduce the influence of unusual data (outliers) in the response (electric field) variables, but are often not sensitive to exceptional predictors (magnetic field) data, which are termed leverage points. This paper proposes an alternative procedure for making reliably robust estimates of MT impedance functions, which simultaneously provide protection from the influence of outliers in both response and input variables. The means for accomplishing this is based on the bounded-influence regression M-estimation and the Hilbert Transform operating on the causal MT impedance functions. In the resulting regression estimates, outlier contamination is removed and the self consistency between the real and imaginary parts of the impedance estimates is guaranteed. Using synthetic and real MT data, it is shown that the method can produce improved MT impedance functions even under conditions of severe noise contamination.
Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments
SUHARJA,SUTARNO
Nusantara Bioscience , 2009,
Abstract: Suharja, Sutarno. 2009. Biomass, chlorophyll and nitrogen content of leaves of two chili pepper varieties (Capsicum annum) in different fertilization treatments. Nusantara Bioscience 1: 9-16. This study aims to determine the influence of various fertilization treatments on biomass, chlorophyll and nitrogen content of leaves from two varieties of chili, Sakti (large chili) and Fantastic (curly chili). The study was conducted in the village of Gatak, Karangnongko sub-district, Klaten District, Central Java in September 2006 to March 2007. The study used a complete block design with two factorial of chili varieties and fertilizer treatment. Fertilization treatments includes no fertilizer (control) (P1); manure 2 kg/plant (P2), manure (1 kg/plant) + chemical fertilizer (ZA, SP-36, KCl = 2: 1: 1) + NPK (P3); and manure (1 kg/plant) + chemical fertilizer (SP-36: KCl = 1:1) + liquid organic fertilizer (P4). Chlorophyll content was measured refers to Harborne (1987), whereas leaf nitrogen concentration was measured with Kjeldahl method. Data were analyzed using ANOVA followed by DMRT. The results showed that on the Fantastic chili fertilizer treatment affected the biomass and chlorophyll a, but gave no effect on chlorophyll b, total chlorophyll and leaf nitrogen. On the curly chili fertilizer treatment effected plant fresh weight, chlorophyll a and total chlorophyll, but gave no effect on dry weight, fresh fruit weight, chlorophyll b and leaf nitrogen. It is, therefore, recommended to use the formulation of manure + chemical fertilizer (SP-36: KCl = 1: 1) + liquid organic fertilizer in the cultivation of chili.Key words: biomass, chlorophyll, leaf nitrogen, chili, Capsicum annum, fertilizing.Abstrak. Suharja, Sutarno. 2009. Biomassa, kandungan klorofil dan nitrogen daun dua varietas cabai (Capsicum annum) pada berbagai perlakuan pemupukan. Nusantara Bioscience 1: 9-16. Penelitian ini bertujuan untuk mengetahui pengaruh berbagai perlakuan pemupukan terhadap biomassa, kandungan klorofil dan nitrogen daun dari dua varietas cabai, Sakti (cabai besar) dan Fantastic (cabai keriting). Penelitian dilakukan di Desa Gatak, Kecamatan Karangnongko, Kabupaten Klaten, Jawa Tengah pada September 2006 sampai Maret 2007. Penelitian menggunakan rancangan blok lengkap dengan dua faktorial yaitu varietas cabai dan perlakuan pemupukan. Perlakuan pemupukan meliputi tanpa pupuk (kontrol) (P1); pupuk kandang 2 kg/tanaman (P2), pupuk kandang (1 kg/tanaman) + pupuk kimia (ZA, SP-36, KCl = 2: 1: 1) + NPK (P3); dan pupuk kandang (1 kg/tanaman) + pupuk kimia (SP-36: KCl = 1:1) + pupuk orga
Biomassa, kandungan klorofil dan nitrogen daun dua varietas cabai (Capsicum annum) pada berbagai perlakuan pemupukan
SUHARJA,SUTARNO
Bioteknologi , 2009,
Abstract: Suharja, Sutarno. 2009. Biomassa, kandungan klorofil dan nitrogen daun dua varietas cabai (Capsicum annum) pada berbagai perlakuan pemupukan. Bioteknologi 6: 11-20. Penelitian ini bertujuan untuk mengetahui pengaruh berbagai perlakuan pemupukan terhadap biomassa, kandungan klorofil dan nitrogen daun dari dua varietas cabai, Sakti (cabai besar) dan Fantastic (cabai keriting). Penelitian dilakukan di Desa Gatak, Kecamatan Karangnongko, Kabupaten Klaten, Jawa Tengah pada September 2006 sampai Maret 2007. Penelitian menggunakan rancangan blok lengkap dengan dua faktorial yaitu varietas cabai dan perlakuan pemupukan. Perlakuan pemupukan meliputi tanpa pupuk (kontrol) (P1); pupuk kandang 2 kg/tanaman (P2), pupuk kandang (1 kg/tanaman) + pupuk kimia (ZA, SP-36, KCl = 2:1: 1) + NPK (P3); dan pupuk kandang (1 kg/tanaman) + pupuk kimia (SP-36: KCl =1:1) + pupuk organik cair (P4). Kadar klorofil diukur merujuk Harborne (1987), sedangkan kadar nitrogen daun diukur dengan metode Kjeldahl. Data dianalisis menggunakan Analisis Varians dilanjutkan DMRT. Hasil penelitian menunjukkan pada cabai Fantastic, perlakuan berbagai macam pemupukan berpengaruh terhadap biomassa dan klorofil a, namun tidak berpengaruh terhadap kandungan klorofil b, total klorofil dan nitrogen daun. Pada cabai Sakti perlakuan pemupukan berpengaruh terhadap bobot segar tanaman, kandungan klorofil a dan total klorofil, namun tidak berpengaruh terhadap bobot kering, bobot buah segar, kandungan klorofil b dan nitrogen daun. Oleh kerena itu direkomendasikan untuk menggunakan formulasi pupuk kandang + pupuk kimia (SP-36: KCl = 1: 1) + pupuk organik cair dalam budidaya cabai.
Effects of Anodizing Parameters in Tartaric-Sulphuric Acid on Coating Thickness and Corrosion Resistance of Al 2024 T3 Alloy  [PDF]
Mohammad Zaki Mubarok, ? Wahab,Sutarno, Soleh Wahyudi
Journal of Minerals and Materials Characterization and Engineering (JMMCE) , 2015, DOI: 10.4236/jmmce.2015.33018
Abstract: 2024 T3 is one of aluminium alloys which are widely used in the aircraft structures. Anodizing of alluminium alloy in tartaric-sulphuric acid (TSA) electrolyte is developed to obtain more environmentally-friendly process and to produce anodize layer with better corrosion resistance. In this research work, the influences of anodizing parameters of Al 2024 T3 in TSA on the thickness, weight and corrosion resistance of the anodize layer are studied. Corrosion resistance test was carried out by conducting salt spray test for 336 hours and anodic polarization measurements using potentiostat. Results of three-factor analysis of variance (ANOVA) demonstrated that the most influencing factor that determines the thickness and weight of the anodize layer is temperature, followed by applied voltage, duration of anodizing, voltage-temperature interaction, interaction of temperature-duration of anodizing, interaction of voltage-temperature-duration of anodizing, and interaction of voltage and duration of anodizing. The pit density and corrosion current density (icorr) were found to be dependent on the coating thickness. The anodize layer with a thickness of higher than 3 μm was not experienced to pitting corrosion during 336 hours of salt spray test.
Genetic variation of Anggoli fish (Pristipomoides multidens) based on allozyme patterns
ENDANG WIGATI,SUTARNO,HARYANTI
Biodiversitas , 2003,
Abstract: The objectives of the research were to study the genetic variation and allozyme band pattern of Pristipomoides multidens from several locations of Indonesian sea based on the pattern of allozyme. Samples of the fish were collected from three geographically different water areas of Bali, Sumbawa and Moluccas. Ten different enzymes, ADH, MDH, LDH, α-GPD, PGM, GPI, IDH, ME, EST and SP were used in this study. Polymorphic loci of genetic variation were in line with the Hardy-Weinberg’s equilibrium. The genetic variation was calculated based on the proportion of polymorphic loci, frequency of allele, amount of allele per locus, and heterozygosity. The results of this research indicate that from the 10 different enzymes, 16 loci were detected, and 3 of them were polymorphic (PGM-1, GPI-1 and EST). Population of Moluccas has 2 polymorphic loci (PGM-1 and GPI-1) by proportion of 13%, however, population from Bali and Sumbawa has only 1 polymorphic locus (EST-1) with the value of 6%. The allelic number per locus was 1.06 – 1.125, while the observed heterozygosity (D) of the populations was 0.005. The fish population of Moluccas is having better genetic variation than that of population from Bali and Sumbawa. The genetic distance between populations was between 0.002 – 0.005. The closest genetic distance is between Bali and Sumbawa (D = 0,002), while the longest genetic distance was resulted between populations of Sumbawa and Moluccas (D = 0.005). Based on the UPGMA cluster analysis for the genetic distances, indicated that there was 2 main geographic groups, (1) Moluccas, and (2) Bali and Sumbawa as single population.
Genetic Diversity and Sequence Variations at Growth Hormone Loci among Composite and Hereford Populations of Beef Cattle
SUTARNO,ALAN J. LYMBERY
Biodiversitas , 2000,
Abstract: A total of 194 Hereford and 235 composite breed cattle from Wokalup Research Station were used in this study. The aims of the study were to: Investigate polymorphisms in the growth hormone gene in the composite and purebred Hereford herds from the Wokalup selection experiment, compare genetic diversity in the growth hormone gene of the breeds, sequencing and compare the sequences of growth hormone loci between composite and purebred Hereford herds with published sequence from Genebank. The genomic DNA was extracted using Wizard genomic DNA purification system from Promega. Two fragments of growth hormone gene were amplified using PCR and continued with RFLP. Each genotype in both loci was sequenced. PCR products of each genotypes were cloned into PCR II, transformed, colonies selection, plasmid DNA extraction continued with cycle sequencing. Polymorphisms were found in both breeds of cattle in both loci of GH-L1 and GH-L2 of the growth hormone gene by PCR-RFLP analysis. Sequencing analysis confirmed the RFLPs data, polymorphism detected using AluI at GH-L1 is due to substitution between leusin/ valine at position 127, while polymorphism at the MspI restriction site was caused by transition of C to T at +837 position.
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