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Search Results: 1 - 10 of 45964 matches for " SU Yong-Quan "
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Molecular cloning and sequence analysis of macrophage migration inhibitory factor(MIF) gene from Thunnus albacares
黄鳍金枪鱼巨噬细胞移动抑制因子(MIF)的克隆与序列分析

MAO Yong,WANG Jun,ZHANG Zhi-wen,WANG Ding,SU Yong-quan,
毛勇
,王军,张之文,王定,苏永全

海洋科学 , 2009,
Abstract: 采用同源克隆的方法、利用RACE技术从黄鳍金枪鱼(Thunnus albacares)肝脏中克隆了其MIF(TaMIF)的cDNA序列.TaMIF的cDNA全长706 bp含一个345 bp的开放阅读框,编码一个长115氨基酸的蛋白质.信号肽预测分析表明TaMIF没有信号肽.序列分析与进化分析表明,黄鳍金枪鱼与近海养殖鱼类MIF的氨基酸序列高度相似、进化地位相近,预示着深海鱼类TaMIF与近海养殖鱼类具有相似的生物学功能.研究结果是对深海鱼类MIF基因信息资料的重要补充.
Development of a Clinically Relevant Animal Model for the Talar Osteonecrosis in Sheep
Chao-Fan Yuan, Jun-Lin Wang, Yong-Quan Zhang, Xiao-Kang Li, Yi Li, Su-Hua Wu, Zhi-Yong Zhang, Zheng Guo
International Journal of Medical Sciences , 2012,
Abstract: There are a lot of reports and reviews about osteonecrosis of the talus (ONT), yet reports about the animal model of ONT to evaluate proper therapeutic approaches are rarely heard. In our study, a novel animal model was established. Pure ethanol was injected into the cancellous bone of sheep's talus. Macroscopic observation, X-ray, CT and histology were performed at two, four, 12 and 24 weeks postoperatively. It was revealed that the trabeculae of talar head began to change their structure after two weeks postoperatively compared to the normal talus. The ONT was obvious at the end of the fourth week, and their outstanding feature was the damage of trabeculae bone and formation of cavities. CT scans and pathological changes of the subjects all showed characteristics of the early stage of osteonecrosis, also the sections of the specimens confirmed necrosis of tali. By 12 weeks, the phenomenon of necrosis still existed but fibrous tissue proliferated prominently and bone reconstruction appeared in certain area. Most specimens (3/4) got late stage necrosis which presented as synarthrosis in X-ray and mass proliferation of fibrous tissue in histology at the end of 24 weeks. The novel animal model of ONT was successful, and it is inclined to deteriorate without any intervention. The study provides us a new way to evaluate various treatments on ONT in laboratory, which may eventually pave way to clinical applications.
Simultaneous Purification of Superoxide Dismutase, Catalase and Hemoglobin from Bovine Erythrocyte Lysate with PEG 600 as Chaperon
以聚乙二醇为层析伴侣同时制备SOD、过氧化氢酶和血红蛋白

WANG Yong-Quan,LU Xiu-Ling,SU Zhi-Guo State Key Laboratory of Biochemical Engineering,
王永权
,路秀玲,苏志国

生物工程学报 , 2005,
Abstract: Superoxide dismutase, catalase and hemoglobin were purified simultaneously from the same batch of bovine erythrocyte lysate. The process involves an initial anion exchange chromatography, followed by a hydrophobic interaction chromatography and gel filtration chromatography. 0.75% polyethylene glycol 600 was added as a purification chaperon before the anion exchange chromatography. The hemoglobin fraction passed through the ion exchange column without being retained. The superoxide dismutase and catalase were adsorbed by the column and were eluted separately during elution. The two eluted fractions containing crude superoxide dismutase and catalase were further purified with hydrophobic interaction chromatography and gel filtration chromatography in sequence. The specific activities of superoxide dismutase and catalase were 15932u/mg and 65918u/mg, respectively. SDS-polyacrylamide gel electrophoresis and gel filtration chromatography were used to analyze the purity of the proteins. The purity of superoxide dismutase, catalase and hemoglobin were 77.6%, 81.9% and 99.9%, respectively. The total recoveries for superoxide dismutase, catalase and hemoglobin were 47.4%, 29.6% and 88.7%, respectively.
CONCEPT AND DETERMINATION OF LIMIT DISPLACEMENTS OF PRIMARY SUPPORT SYSTEM OF RAILWAY TUNNEL
铁路隧道初期支护极限位移的意义及确定

ZHU Yong-quan,ZHANG Su-min,JING Shi-ting,
朱永全
,张素敏,景诗庭

岩石力学与工程学报 , 2005,
Abstract: Concept of limit displacements of primary support system of railway tunnel are presented,and the method for determining the limit displacements is introduced systematically. The continuum model is adopted,and the cross section pattern and the material properties are used according to the design standard for railway tunnel lining. The limit displacements of the primary support system of railway tunnels are simulated and calculated for the tunnels of different depths in surrounding rocks of different classification. The calculation results are further treated in a statistical manner and compared with the allowable displacements. The limit displacements for single track tunnels and for double track tunnels are discriminated. Criteria are established respectively for crown settlement,horizontal convergence at arch springer and at wall foot. The depth ranges in the original codes are adopted,and the displacement range values are determined by combining the two sets of limit values. A series of limit displacement data are finally determined synthetically,which are consistent with the current displacement criterion for railway tunnel stability.
STUDY OF CULTURED DAIQU AND MINYUE POPULATIONS LAGER YELLOW CROAKER PSEUDOSCIAENA CROCEA AND ITS HYBRID FILIAL GENERATIONS USING SSR TECHNOLOGY
闽粤群和岱衢群养殖大黄鱼(Pseudosciaena crocea)及其杂交子代遗传差异的 SSR 分析

HUANG Zhen-Yuan,SU Yong-Quan,ZHANG Jian-She,WU Chang-Wen,
黄振远
,苏永全,张建设,吴常文

海洋与湖沼 , 2011,
Abstract: 采用SSR方法进行了闽粤群(MY)和岱衢群(DQ)及其正交和反交大黄鱼的遗传差异的研究。结果表明, Shannon’s 多样性指数为 DQ 群高于 MY 群, 分别为 2.14、2.04; 反交群高于正交群分别为2.13、2.03; 杂交群体中为 2.24 与其亲体群的相似(2.22)。亲体群间基因分化系数 Fst 值为 0.020, 低于杂交后代的 0.026。AMOVA 显示, 亲体群和后代群基因差异均很低, 且亲体群基因分化度低于和杂交后代群, Fst 值分别为 0.011、0.032。Nei 遗传距离显示子代间遗传距离大与亲代代间的遗传距离分别为 0.39、0.22。UPGMA 聚类将其分为 3 组, 其中 DQ 群与 MY 群遗传距离最近。可以认为养殖的DQ群与MY群大黄鱼间遗传背景差异较低, 其中DQ族遗传背景较好, 杂交后代群的基因多样性和基因差异性稍有提高, 其中反交效果较好。
GENETIC DIVERSITY IN POPULATIONS OF CISTOPUS SP.BASED ON THE SEQUENCES OF MITOCHONDRIAL 16S rRNA AND CO Ⅰ GENE FRAGMENTS
东南沿海小孔蛸(Cistopus sp.)线粒体序列的比较研究

YANG Qiu-Ling,LIN Xiang-Zhi,ZHENG Xiao-Dong,SU Yong-Quan,
杨秋玲
,林祥志,郑小东,苏永全

海洋与湖沼 , 2009,
Abstract: 采用线粒体DNA 16S rRNA和COⅠ基因序列对福建、浙江沿海分布的5个小孔蛸(Cistopus sp.)群体进行了遗传多样性研究。结果表明,序列总长度分别为497-501bp(16S rRNA)和652bp(COⅠ);小孔蛸5个群体的序列碱基组成均显示出较高的A+T含量(16S rRNA基因72.1%,COⅠ基因65.9%)。16S rRNA基因片段在种内和群体间个体差异均较小,5个群体共检测到8个变异位点(其中7个插入/缺失位点);COⅠ基因序列不存在插入/缺失位点,群体内个体间变异位点数为21个。系统进化树表明,宁德群体和象山群体与其它三个群体的亲缘关系较远,但存在基因交流。线粒体16S rRNA和COⅠ基因在群体间无显著多态性分布。
Analysis of inosine monophosphate in six fish musculatures
6种鱼类肌肉组织肌苷酸的检测分析

LIU Xu,WANG Jun,ZHANG Wen,DU Jia-ying,SU Yong-quan,
刘旭
,王军,张纹,杜佳莹,苏永全

海洋科学 , 2008,
Abstract: 采用高效液相色谱技术,在国内首次测定了条纹斑竹鲨(Chilocsyllium plagiosum)、大黄鱼(Pseudosci-aena crocea)、鳜(Siniperca chuatsi)、青石斑鱼(Epinephelus awoara)、牙鲆(Paralichthys olivaceus)和乌塘鳢(Bostrichthys sinensis)等6种鱼类新鲜和冻存肌肉组织中肌苷酸含量。结果表明:(1)不同鱼类肌肉组织中肌苷酸质量比不同,牙鲆新鲜肌肉组织中肌苷酸质量比最高,为4.214 mg/g,其余5种鱼类为2.365~3.634 mg/g。(2)10 d冻存试验中,经-70℃超低温冻存,6种鱼类冻存肌肉组织中肌苷酸质量比均高于新鲜肌肉组织,大黄鱼肌肉组织冻存1 d肌苷酸质量比达最大值,鳜肌肉组织冻存10 d肌苷酸质量比呈上升态势未出现最大值,其余5种鱼类肌肉组织肌苷酸质量比均在冻存第5天达到最大值。长时间的冻存将会降低食用鱼肉中肌苷酸质量比,导致鲜味下降,但经1周左右短时间的冻存后可明显提高鱼肉中肌苷酸质量比,增加鲜味,提高食用价值。
Histologic comparative study of muscles of several commercial fishes in coastal waters of the southeast China seas
东南沿海几种经济鱼类肌肉组织学比较研究

XU Bin,SU Yong-quan,ZHANG Wen,GUO Ming-lan,WANG Jun,
徐斌
,苏永全,张纹,郭明兰,王军

海洋科学 , 2009,
Abstract: Muscles of 5 large yellow croaker (Pseudosciaena crocea) strains and 5 other species of fish in the southeast coastal waters were observed by using paraffin section. The results showed that among large yellow croaker strains, there was no significant difference in muscle fibers between wild fish and their cultured progenies, but gynogenesis strain and its hybrid progenies showed a greater density of muscle fibers than other strains. There were also evident differences between species, and the differences reflected the genetic features and living mode of the fish species.
A Series of New Studies on Common Chinese Words
汉语俗字新考

ZHANG Yong-quan,
张涌泉

浙江大学学报(人文社会科学版) , 2005,
Abstract: 宋代以后的一些字典辞书,诸如<龙龛手镜>、<四声篇海>、<字汇>、<字汇补>、<康熙字典>,以及今人编的<汉语大字典>、<中华字海>等,都收载了大量有音无义或音义全无以及与正字关系不明的疑难俗字.由于种种原因,汉语俗字的研究长期以来没有得到人们应有的重视,所以上述字典中的这些疑难俗字也很少有人问津.这种情况不但对载有这些疑难俗字的古籍的解读带来了困难,也对现代大型字典编纂的质量带来了严重的负面影响.本文在<汉语俗字丛考>的基础上,依据敦煌出土文献等大量第一手资料,对<汉语大字典>等大型字典中的二十多个疑难俗字进行了考辨,并纠正了旧辞书辨释方面的一些错误.
Reaction Kinetics and Mechanism of Photocatalytic Degradation of Gaseous Toluene
气相甲苯光催化降解反应动力学及机理

YIN Yong-quan,ZHENG Yan,SU Yuan-cheng,CUI Quan,CUI Zhao-jie,
殷永泉
,郑艳,苏元成,崔瑔,崔兆杰

过程工程学报 , 2009,
Abstract: Chemical kinetics and intermediates in photocatalytic degradation of gaseous toluene were investigated under UV irradiation with TiO2. According to the intermediates analyzed by GC/MS, possible mechanism for photocatalytic degradation of gaseous toluene was proposed. The result showed that the degradation of toluene followed the first-order chemical kinetics. With the increase of initial toluene concentration, the reaction rate of photocatalytic degradation of toluene increased and the reaction rate decreas...
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