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Search Results: 1 - 10 of 225038 matches for " SOUTO ELIEZER R. "
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Análise molecular de segmento do RNA2 de comovirus isolados de soja no estado do Paraná
SOUTO, ELIEZER R.;ALMEIDA, áLVARO M. R.;BIANCHINI, ANéSIO;SARTORI, FáBIO;CALVO, éBERSON S.;
Fitopatologia Brasileira , 2002, DOI: 10.1590/S0100-41582002000500014
Abstract: in bean (phaseolus vulgaris) producing areas of paraná state, bean rugose mosaic virus (brmv) has been found in mixed infections with bean golden mosaic virus (bgmv) increasing the severity of symptoms and crop losses. the association of cowpea severe mosaic virus (cpsmv) with bud blight symptoms in soybean (glycine max) plants was recently observed in paraná for the first time. in this work rt-pcr products of 600 bp of brmv-pr rna2 and 594 bp of cpsmv-pr rna2 were cloned and sequenced. the amino acid sequences coded by the rna2 segment from these isolates were compared with sequences of the same rna2 genomic region of six comovirus species from the genbank. the cpsmv-pr showed 85% homology with the cpsmv rna2 from the genbank. moreover, the brmv-pr isolate showed 39 and 44% homology with cpsmv and bean pod mottle virus (bpmv), respectively. this work shows, for the first time, partial sequencing data for brmv. this data may be helpful for the complete molecular characterization of this virus and for establishing strategies to obtain virus resistant plants.
Simultaneous detection of Brazilian isolates of grapevine viruses by TaqMan real-time RT-PCR
Carla R. Dubiela,Thor V.M. Fajardo,Eliezer R. Souto,Osmar Nickel
Tropical Plant Pathology , 2013,
Abstract: The aim of this work was to evaluate the efficiency of real-time RT-PCR for detection of different isolates of ten important virus species that infect grapevines in Brazil: Grapevine leafroll-associated virus (GLRaV-1, -2, -3 and -5), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine virus D (GVD), Grapevine rupestris stem pitting-associated virus (GRSPaV), Grapevine fleck virus (GFkV) and Grapevine fanleaf virus (GFLV). The reactions consisted of individual (simplex) and simultaneous (duplex) virus detections. Thirty six grapevine accessions, regenerated after thermotherapy and tissue culture treatments, have been analysed. All the above-mentioned viruses were sensitively detected in simplex reactions in samples infected with different virus isolates. Specifically to GLRaV-1 it was necessary to mix reagents refered by different sources to achieve the amplification. GVA, GRSPaV, GLRaV-2 and GLRaV-3 combined with GVB, GFLV, GFkV, GVD and GLRaV-5 were accurately detected in duplex trials. It was shown, that real-time RT-PCR (TaqMan) is able to efficiently detect different local virus species and isolates.
Mosaic in Senna occidentalis in southern Brazil induced by a new strain of Soybean mosaic virus
ALMEIDA áLVARO M. R.,SAKAI JUNICHI,SOUTO ELIEZER R.,KITAJIMA ELLIOT W.
Fitopatologia Brasileira , 2002,
Abstract: Plants of Senna occidentalis (sin. Cassia occidentalis) with mosaic symptoms were collected near a soybean (Glycine max) field where some plants exhibited symptoms of mosaic and blistering. A preliminary examination of leaf tissue from diseased S. occidentalis by electron microscopy revealed the presence of pinwheel inclusions as well as long flexuous particles, indicating the presence of a potyvirus. Host range, serology, and amino acid sequence from this potyvirus were similar to those from other Brazilian isolates of Soybean mosaic virus (SMV). The 3'- terminal region of the genomic RNA was cloned and a cDNA sequence of 1.9 kb upstream of the poly (A) tract was determined. The sequence contains a single open reading frame and a 3'- non-translated region (NTR) of 259 bp. The nucleotide sequence of the CP gene of SMV-Soc was 98% identical to that of Brazilian isolates SMV-B, SMV-L, and SMV-FT10. The percentage of nucleotide identity of their 3'-NTR's was 91, 98, and 99% in relation to SMV-L, SMV-B, and SMV-FT10, respectively. In contrast to other Brazilian SMV isolates studied, SMV-Soc was able to infect sunflower (Helianthus annuus). Based on these results, the S. occidentalis isolate was identified as a new strain of SMV belonging to the SMV strain, group G5 and was named SMV-Soc. This is the first report of naturaly occurring SMV infecting plants of S. occidentalis in Brazil, adding this weed as a new source of SMV in the field.
Análise molecular de segmento do RNA2 de comovirus isolados de soja no estado do Paraná
SOUTO ELIEZER R.,ALMEIDA áLVARO M. R.,BIANCHINI ANéSIO,SARTORI FáBIO
Fitopatologia Brasileira , 2002,
Abstract: Nas áreas produtoras de feij o (Phaseolus vulgaris) do Estado do Paraná observa-se anualmente a ocorrência do vírus do mosaico em desenho do feijoeiro (Bean rugose mosaic virus, BRMV), principalmente em infec es mistas com o vírus do mosaico dourado do feijoeiro (Bean golden mosaic virus, BGMV), acarretando maior severidade de sintomas e causando perdas na produ o. Recentemente constatou-se a presen a do vírus do mosaico severo do caupi (Cowpea severe mosaic virus, CPSMV) associado a sintomas de queima do broto em planta es de soja (Glycine max) na regi o de Londrina, sendo este um fato novo no Estado. Neste trabalho, parte do RNA2 de dois comovirus isolados de soja no Paraná foram clonados e sequenciados, sendo 600 pares de bases (pb) do BRMV-PR e 594 pb do CPSMV-PR. Posteriormente, as seqüências correspondentes de aminoácidos foram comparadas com seis seqüências de vírus do gênero Comovirus depositadas no GenBank. Com base nestes dados observou-se que o segmento do RNA2 do isolado CPSMV-PR apresentou homologia de 85% com parte de uma seqüência já conhecida do RNA2 do CPSMV, enquanto que o segmento do RNA2 do isolado BRMV-PR apresentou homologia de 39% com o CPSMV, e de 44% com o Bean pod mottle virus (BPMV). Este trabalho apresenta pela primeira vez dados de sequenciamento parcial do BRMV, o que poderá contribuir para sua completa caracteriza o molecular e para o estabelecimento de estratégias para obten o de plantas resistentes ao vírus.
Detec??o do Southern bean mosaic virus no Paraná, e separa??o do Bean rugose mosaic virus em feijoeiro
Gasparin, Marcos D. G.;Bianchini, Anésio;Souto, Eliezer R. de;Gaspar, José Osmar;Almeida, álvaro M. R.;Kitajima, Elliot W.;
Fitopatologia Brasileira , 2005, DOI: 10.1590/S0100-41582005000100013
Abstract: plants of bean (phaseolus vulgaris), showing symptoms of stunt, stem necrosis and chlorotic mosaic, similar to those induced by virus infection were found in a bean field in londrina, paraná. electron microscopy examinations showed isometric virus particles in the cell cytoplasm. double immunodifusion serological tests with antiserum for southern bean mosaic virus (sbmv) gave positive results when tested against plant sap from infected bean plants. the virus was purified and the molecular mass of its coat protein was estimated as 30 kda, the expected value for the coat protein of viruses from the genus sobemovirus. the host range of the virus was restricted to bean and some soybean (glycine max) cultivars. it was possible to separate two isometric viruses commonly found in bean based on the immunity reaction of crotalaria sp., chenopodium quinoa and mucuna deeringiana to sbmv, and on the susceptibility reaction of the same hosts to bean rugose mosaic virus (brmv).
Mosaic in Senna occidentalis in southern Brazil induced by a new strain of Soybean mosaic virus
ALMEIDA, áLVARO M. R.;SAKAI, JUNICHI;SOUTO, ELIEZER R.;KITAJIMA, ELLIOT W.;FUKUJI, TATIANA S.;HANADA, KAORU;
Fitopatologia Brasileira , 2002, DOI: 10.1590/S0100-41582002000200005
Abstract: plants of senna occidentalis (sin. cassia occidentalis) with mosaic symptoms were collected near a soybean (glycine max) field where some plants exhibited symptoms of mosaic and blistering. a preliminary examination of leaf tissue from diseased s. occidentalis by electron microscopy revealed the presence of pinwheel inclusions as well as long flexuous particles, indicating the presence of a potyvirus. host range, serology, and amino acid sequence from this potyvirus were similar to those from other brazilian isolates of soybean mosaic virus (smv). the 3'- terminal region of the genomic rna was cloned and a cdna sequence of 1.9 kb upstream of the poly (a) tract was determined. the sequence contains a single open reading frame and a 3'- non-translated region (ntr) of 259 bp. the nucleotide sequence of the cp gene of smv-soc was 98% identical to that of brazilian isolates smv-b, smv-l, and smv-ft10. the percentage of nucleotide identity of their 3'-ntr's was 91, 98, and 99% in relation to smv-l, smv-b, and smv-ft10, respectively. in contrast to other brazilian smv isolates studied, smv-soc was able to infect sunflower (helianthus annuus). based on these results, the s. occidentalis isolate was identified as a new strain of smv belonging to the smv strain, group g5 and was named smv-soc. this is the first report of naturaly occurring smv infecting plants of s. occidentalis in brazil, adding this weed as a new source of smv in the field.
Effect of crop rotation on specialization and genetic diversity of Macrophomina phaseolina
Almeida, álvaro M.R.;Sosa-Gomez, Daniel R.;Binneck, Eliseu;Marin, Silvana R.R.;Zucchi, Maria I.;Abdelnoor, Ricardo V.;Souto, Eliezer R.;
Tropical Plant Pathology , 2008, DOI: 10.1590/S1982-56762008000400001
Abstract: charcoal rot, caused by macrophomina phaseolina, is one of the most important diseases of soybean. genetic variability among soybean isolates has been observed but the effect of host specialization on genetic variability has not been reported. in this work, isolates from soybean, corn and sunflower were evaluated based on cross inoculations and number of microsclerotia/g of roots. the highest and lowest significant (p<0.005) production of microsclerotia was obtained in soybean (863 microsclerotia/g of roots) and sunflower (578 microsclerotia/g of roots), respectively, regardless of the origin of the isolate. additionally, the effect of a 20-year crop rotation on genetic variability based on rapd was studied. eighty-nine isolates from five populations were obtained from soil samples under four crop rotation systems and an uncropped soil. seven clusters were obtained considering a similarity level of 85%. analysis indicated that m. phaseolina is a highly diverse species and also revealed a strong effect of the rotation system on genetic diversity. amova was conducted for the rapd data. from the total genetic variability, 21% (p<0.0001) could be explained by the differences between populations while 79% could be explained by differences within populations ( φst = 0.2110; p<0.0001). the mean coefficient of gene differentiation (gst) estimated among the five populations indicated 27% of differentiation between populations similar to the amova results where φst= 0.2110. total gene diversity estimated indicated high levels of variability (ht =0.3484). results suggest that genetic differentiation of m. phaseolina can be altered by crop rotation.
Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil
Almeida, álvaro M. R.;Sakai, Junichi;Hanada, Kaoru;Oliveira, Thalita G.;Belintani, Priscila;Kitajima, Elliot W.;Souto, Eliezer R.;Novaes, Tanara G. de;Nora, Paulo S.;
Fitopatologia Brasileira , 2005, DOI: 10.1590/S0100-41582005000400005
Abstract: a virus was isolated from soybean (glycine max) plants with symptoms of dwarfing and bud blight in wenceslau braz county, paraná, brazil. the host range and properties resembled those of tobacco streak virus (tsv). the purified virus showed three peaks in a frozen sucrose gradient. antiserum was produced and the virus was serologically related to tsv. electron microscopy detected 28 nm spherical particles. coat protein (cp) had a mr of 29.880 da. a fragment of 1028 nt was amplified, cloned and sequenced. one open reading frame with 717 nt was identified and associated to the cp. the cp gene shared 83% identity with the sequence of tsv cp from white clover (trifolium repens) (genbank caa25133). this is the first report of the biological and molecular characterization of tsv isolated from soybeans. it is proposed that this isolate be considered a strain of tsv named tsv-br.
Detec??o do Sugarcane mosaic virus no Paraná e limpeza somaclonal por cultura de tecidos
Barboza, Ant?nio A.L.;Silva Júnior, Hélio M.;Souto, Eliezer R.;Silva, Clandio M.;Marcuz, Fernanda S.;Vieira, Rafael A.;
Fitopatologia Brasileira , 2007, DOI: 10.1590/S0100-41582007000400010
Abstract: an isolate of sugarcane mosaic virus (scmv) associated with mosaic symptoms in the rb925268 sugarcane clone was identified in paraná state. axilary buds of 4 to 8 mm used as explants were regenerated into plantlets using ms medium with and without ribavirin antiviral, at concentrations of 10 to 60 mg/l. ribavirin was toxic to sugarcane at all concentrations, resulting in plant death at concentrations of 30 mg/l or higher. the complete elimination of scmv was obtained in ms medium with 25 mg/l ribavirin concentration, confirmed by sap inoculation into sorghum bicolor cv. rio and by rt-pcr.
Immunocapture-RT-PCR detection of Cassava common mosaic virus in cassava obtained from meristem-tip culture in Paraná state
Silva, Jaqueline M.;Carnelossi, Patrícia R.;Bijora, Taise;Facco, Cassiele U.;Picoli, Marcelo H.S.;Souto, Eliezer R.;Oliveira, Arildo J.B.;Almeida, álvaro M.R.;
Tropical Plant Pathology , 2011, DOI: 10.1590/S1982-56762011000500001
Abstract: a virus survey conducted in the northwest region of paraná, the main cassava-producing region of that state, showed cassava common mosaic virus (cscmv) to be widespread, infecting more than 90% of plants from all cassava cultivars. a cscmv isolate was purified and used to raise a high-titer (1/1.000) polyclonal antiserum for indexing plants produced from meristem-tip culture, using pta-elisa. from an initial production of 110 plants of cultivar olho junto, 31 remained infected as indicated by pta-elisa. to improve the sensitivity of virus detection, an immunocapture-rt-pcr (ic-rt-pcr) protocol was established. virus-specific igg, purified and combined with a primer set for the genus potexvirus, could readily detect cscmv in cassava crude leaf extracts. ic-rt-pcr products of 750 bp were amplified from six out of 35 plants previously tested as virus-negative by pta-elisa. sequence analysis of cloned ic-rt-pcr products confirmed they were part of the cscmv replicase gene, indicating that the virus was still present after thermotherapy and meristem-tip culture. pta-elisa enabled initial screening of virus-positive cassava, reducing the number of plants to be further analyzed by ic-rt-pcr. though cscmv has been considered of minor importance, its widespread nature, as noticed in paraná, indicates the need for adoption of effective control measures.
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