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Search Results: 1 - 9 of 9 matches for " SEPTELIA INAWATI WANANDI "
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AKTIVITAS SPESIFIK MANGANESE SUPEROXIDE DISMUTASE (MnSOD) DAN KATALASE PADA HATI TIKUS YANG DIINDUKSI HIPOKSIA SISTEMIK: HUBUNGANNYA DENGAN KERUSAKAN OKSIDATIF
Masagus Zainuri,Septelia Inawati Wanandi
Media of Health Research and Development , 2012,
Abstract: Enzim MnSOD dan katalase adalah antioksidan endogen yang dapat menangkap dan menguraikan radikal bebas di dalam sel menjadi zat yang kurang reaktif. Stres oksidatif menyebabkan kerusakan oksidatif lipid yang dapat dideteksi dengan peningkatan kadar Malondialdehyde (MDA) dalam sel. Hipoksia sistemik kronik adalah keadaan yang disebabkan berkurangnya asupan oksigen secara sistemik dalam jangka waktu lama. Keadaan ini dapat menyebabkan stres oksidatif yang berakibat pada kerusakan oksidatif sel dalam berbagai jaringan. Penelitian kami terdahulu membuktikan bahwa terjadi respon yang berbeda pada berbagai jaringan tikus yang diinduksi hipoksia sistemik kronik. Penelitian ini bertujuan menganalisis aktivitas spesifik enzim MnSOD, dan katalase pada sel hati tikus yang diinduksi hipoksia sistemik kronik dan hubungannya dengan kerusakan oksidatif. Jaringan hati tikus jantan strain Sprague Dawley (Rattus novergicus L), diinduksi hipoksia sistemik kronik selama 1,7,14 dan 21 hari. Pada homogenat hati tikus dilakukan pemeriksaan aktivitas spesifik MnSOD, aktivitas spesifik katalase dan kadar MDA. Hasil pengamatan menunjukkan terjadi penurunan aktivitas spesifik MnSOD dan katalase yang bermakna (p<0,05) disertai peningkatan kadar MDA pada hari ke-7 induksi hipoksia sistemik. Selain itu didapat hubungan negatif antara aktivitas spesifik MnSOD dan katalase dengan kadar MDA, sehingga MnSOD dan katalase diduga berperan dalam mencegah kerusakan oksidatif lipid dalam sel hati. Dari hasil penelitian ini dapat disimpulkan bahwa induksi hipoksia sistemik hingga 7 hari menyebabkan kerusakan oksidatif pada sel hati ditandai dengan peningkatan MDA dan diikuti dengan respons penurunan aktivitas antioksidan MnSOD dan katalase. Kata Kunci : MnSOD, katalase, kerusakan oksidatif, malondialdehyde, hati.
Anti free radical & anti inflammatory effect of rebamipide in chronic gastritis  [PDF]
Marcellus Simadibrata, Ari Fahrial Syam, Aziz Rani, Septelia Inawati Wanandi, Achmad Fauzi, Murdani Abdullah
Open Journal of Gastroenterology (OJGas) , 2013, DOI: 10.4236/ojgas.2013.31011
Abstract:

Background/Aim: Free radicals have a role in the development of chronic gastritis. The aim of this study to know the effect and efficacy of rebamipide on free radicals in chronic gastritis. Method: Forty five patients in the division gastroenterology Cipto Mangunkusumo Hospital Jakarta 2009-2010 with moderate and severe gastritis endoscopically were included in this study. Before and after rebamipide treatment the patient were performed endoscopical examination and were taken 5 biopsies for histopathological examination and free radicals (MDA & Carbonyl Compound) examination. All patients were given rebamipide 100 mg three times a day for 28 days. Data were analyzed with t test or wilcoxon signed rank test. Exclusion: GERD, Peptic ulcer, PPI treatment, NSAID consumption etc. The symptoms were recorded on day-0 and day-28. The severity symptoms were measured by VAS. Result: The mucosal damage on day-0 was 2.268 ± 0.45 vs day-28 was 1.707 ± 0.78 (P < 0.001). The antrum neutrophil: day-0: 0.12 ± 0.46 vs day-28: 0.10 ± 0.37 (P = 0.710) and corpus neutrophil: day-0: 0.12 ± 0.40 vs day-28: 0.07 ± 0.26 (P = 0.421). The mean endoscopical mucosal severity score was decreased significantly on day- 28 compared to day-0 (1.707 ± 0.78 vs 2.268 ± 0.45; P < 0.05). The other histopathological appearances between day-0 and day-28 were not different. Rebamipide can reduce the mean of MDA from 5.28 ± 3.54 on day-0 to 4.15 ± 2.71 on day-28 (P = 0.047). The mean of carbonyl compound on day-0 was 4.14 ± 3.01 and on day-28 was 5.12 ± 2.71 (P = 0.642). Conclusion: Rebamipide significantly reduced the extend of symptoms associated with chronic gastritis. The improvement in symptoms was associated with the decreased of endoscopic severity score and the mean gastric mucosal malondialdehyde (MDA)

Relative Expression of HIF-1α mRNA in Rat Heart, Brain and Blood During Induced Systemic Hypoxia
Septelia Inawati Wanandi,Reni Paramita,Syarifah Dewi
Makara Seri Sains , 2009,
Abstract: Hypoxia is a pathological condition in which the body as a whole or region of the body (tissue or cell) deprived of adequate oxygen supply. The transcriptional regulator hypoxia inducible factor-1 (HIF-1) is an essential mediator of O2 homeostasis. Unlike the β sub unit (HIF-1β), the activity of HIF-1α is controlled in an oxygen-dependent manner. It has been reported that the stability and expression of HIF-1α during hypoxia is remarkably higher than those under normoxic conditions.The aim of this study was to analyze the adaptive tissue responses during induced systemic hypoxia by comparation of relative expression of mRNA HIF-1α in rat heart, brain and blood. Twenty-five male Sprague Dawley rats were subjected to systemic hypoxia by placing them in the hypoxic chamber supplied by 8-10% of O2 for 0, 1, 7, 14 and 21 days, respectively. The relative expression level of HIF-1α mRNA in brain, heart and leucocyte cells were analyzed using quantitative RT-PCR assay (Real Time PCR) based on Pfaff's formula. This study demonstrates that the increased of relative expression of HIF-1α mRNA during induced systemic hypoxia reached its maximum level at day 7 (in heart) or at day 14 (in brain), whereas in leucocyte cells the stimulation of HIF-1α expression was intensively maintained up to 21 days although the expression has reached the remarkably high level. We could conclude that HIF-1α as an oxygen sensing during systemic hypoxia has different capacity and sensitivity in brain, heart and blood tissues, due to the importance of oxygen homeostasis in each tissue.
Isolation and Characterization of Chelonia mydas Myoglobin
MOHAMAD SADIKIN,RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO
HAYATI Journal of Biosciences , 2012,
Abstract: Green sea turtle – Chelonia mydas is a lung respiration animal that is able to dive and stay uder sea water for hours without needing to surface for oxygen. As oxygen supply in muscles is assured by myoglobin, we propose to study some characteristic of this muscle protein in green sea turtles. To achieve this objective, pure green sea turtle myoglobin has to be made available. Therefore, our first task is to purify this muscle protein from green sea turtles. Skeletal muscles from 3 green sea turtle hatchlings were studied icroscopically and biochemically. Microscopy observation showed a general structure of striated muscle. Biochemical studies revealed that green sea turtle myoglobin could be more purely isolated to a certain degree by ephadex G-75 gel filtration and purified by immunoaffinity gel chromatography rather than direct purification by DEAE-Sepharose ion exchange chromatography technique. SDS-PAGE analysis showed that green sea turtle myoglobin ran together with horse myoglobin as 17 kDa molecular weight proteins.
Blood Gasses Contents of Green Turtle (Chelonia mydas) Hatch Treated by Different Temperatures
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2011,
Abstract: The aim of this research was to gain the profile of blood gasses of green turtle (Chelonia mydas) hatch. Blood gas of the green turtle was analysed after exposuring them at 28 oC and 50% of humidity for 24 hours in a pvc tube and at 34 oC under sunlight exposured with 47% of humidity for 30 minutes. The result showed the different values of blood gas contents. This result showed indication of metabolism activities and poikilothermic adaptation of green turtle hatch. This information can be used to support for turtle hatchery in Indonesia.
Myoglobin Expression in Chelonia mydas Brain, Heart and Liver Tissues
RINI PUSPITANINGRUM,SEPTELIA INAWATI WANANDI,RONDANG ROEMIATI SOEGIANTO,MOHAMAD SADIKIN
HAYATI Journal of Biosciences , 2010,
Abstract: An understanding of the underpinning physiology and biochemistry of animals is essential to properly understand the impact of anthropogenic changes and natural catastrophes upon the conservation of endangered species. An observation on the tissue location of the key respiratory protein, myoglobin, now opens up new opportunities for understanding how hypoxia tolerance impacts on diving lifestyle in turtles. The respiratory protein, myoglobin has functions other than oxygen binding which are involved in hypoxia tolerance, including metabolism of reactive oxygen species and of the vascular function by metabolism of nitric oxide. Our work aims to determine whether myoglobin expression in the green turtle exists in multiple non muscle tissues and to confirm the hypothesis that reptiles also have a distributed myoglobin expression which is linked to the hypoxiatolerant trait. This initial work in turtle hatch Chelonia mydas confirms the presence of myoglobin transcriptin brain, heart and liver tissues. Furthermore, it will serve as a tool for completing the sequence and generating an in situ hybridization probe for verifying of cell location in expressing tissues.
EXPRESSION AND GENOTYPE OF MANGANESE SUPEROXIDE DISMUTASE IN LUNG CANCER CELLS OF SMOKER PATIENTS
Septelia Inawati Wanandi,Elisna Syahruddin,Reni Paramita,Ay Ly Margareth
Makara Seri Kesehatan , 2009,
Abstract: Although the most common cause of lung cancer is long-term exposure to tobacco smoke, the role of genetic factor for the cell defense mechanism, such as MnSOD, should also be considered. This study aims to analyze the expression and genotype of MnSOD in lung cancer cells of smoker patients. Samples were normal and lung cancer cells of patients operated in Persahabatan Hospital from May to December 2008, as well as lung cancer cells extracted from FFPE collection. Leukocyte cells of healthy smoker subjects were used as controls. The MnSOD mRNA expression was analyzed using Real Time RT-PCR and the specific activity using xantin oxidase inhibition assay. The genotyping was performed using PCR-RFLP. The result showed that the MnSOD specific activity in lung cancer of smoker patients is higher than in leukocyte cells of smoker controls. Compared to the expression of MnSOD in the normal lung cells of patients, in the lung cancer cells the level of MnSOD mRNA was lower, whereas its specific activity was higher (1.988 times). The samples from lung cancer patients have a Val/Val genotype frequency of 100%. In this study, we could conclude that MnSOD expression is altered in lung cancer cells.
Towards a New World Order
Jusuf Wanandi
Kasarinlan : Philippine Journal of Third World Studies , 1992,
Abstract: Teilhard de Chardin has shown that the world and man evolve in stages toward perfection. Each stage shows an improvement from the previous stage. The evolution of man is relatively recent compared to the evolution of the world and the universe. Therefore, man's evolution towards perfection is still going to take a long time. In this perspective, it appears to be too premature for Francis Fukuyama to predict the end of history with the victory of the ideas of liberalism-capitalism-democracy over socialism-communism-autocracy.
The Effect of Inai (Lawsonia inermis Linn) Leaves Extract on Blood Sugar Level: An Experimental Study
Syamsudin,Inawati,Hendig Winarno
Research Journal of Pharmacology , 2013,
Abstract: This is a study of the effect of Inai (Lawsonia inermis Linn) leaves extract on glucose, total cholesterol and triglyceride of blood of mice which was induced by alloxan of 70 mg kg 1 BW. Inai leaves extract was obtained by the percolation of dried inai leaves using 70% ethanol. Sample treatments were done at day of 0, 3, 7 and 14th after the mice underwent the hyperglycaemic condition. The results showed that the feeding of 0.8 g kg 1 BW of inai extract decreased the glucose concentration from 194 mg dL 1 to normal condition after the 14th day. A similar result occured on total cholesterol concentration in which the total cholesterol concentration decreased from 148.9-55.3 mg dL 1 and triglyceride concentration decreased from 225.7-76.9 mg dL 1.
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