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Search Results: 1 - 10 of 325301 matches for " S. Raveendar "
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Improved Agrobacterium Mediated Transformation in Cowpea Vigna unguiculata L. Walp.
S. Raveendar,S. Ignacimuthu
Asian Journal of Plant Sciences , 2010,
Abstract: Agrobacterium tumefaciens strain LBA4404 carrying binary vector pCAMBIA1305.1, with a hygromycin phosphotransferase gene (hpt) and a β-glucuronidase (GUS) gene interrupted with an intron, was used for transformation of Vigna unguiculata cotyledonary node explants. High concentration of acetosyringone (200 μM) in the Agrobacterium culture and co-cultivation medium with 1 g L-1 L-cysteine, 250 mg L-1 Na-thiosulphate and 150 mg L-1 dithiothretiol (DTT) proved to be indispensable for successful transformation. Three days old cotyledonary nodes were used for transformation studies. Binary vector pCAMBIA 1305.1 proved to be very efficient for transformation. Stable transformation with 1.61% efficiency was achieved using optimized conditions. Transformed green shoots were rooted on medium containing hygromycin. Transformed shoots tested positive for hpt gene by polymerase chain reaction. GUS activity was detected in cotyledonary nodes and leaves of the putative transformants. Southern analysis of putative transformants showed the integration of hpt into the plant genome.
Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
Sebastin Raveendar,Gi-An Lee,Young-Ah Jeon,Yun Jeong Lee,Jung-Ro Lee,Gyu-Taek Cho,Joon-Hyeong Cho,Jong-Hyun Park,Kyung-Ho Ma,Jong-Wook Chung
Molecules , 2015, DOI: 10.3390/molecules20011543
Abstract: The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch ( Vicia sativa subsp. s ativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.
Study of the Structural and Electrical Properties of Cr-Doped BiFeO3 Ceramic  [PDF]
S. S. Arafat, S. Ibrahim
Materials Sciences and Applications (MSA) , 2017, DOI: 10.4236/msa.2017.810051
Abstract: Multiferroic BiFe1-xCrxO3 (x = 0.2 and 0.4) ceramics were synthesized in a single phase. The effects of Cr3+ substitution on the crystal structure, dielectric permittivity and leakage current were investigated. Preliminary X-ray structural studies revealed that the samples had a rhombohedral perovskite crystal structure. The dielectric constant ε' significantly increased while the dielectric loss tanδ was substantially decreased with the increase in Cr3+ substitution. The temperature effect on the dielectric properties exhibited an anomaly corresponding to magneto-electric coupling in the samples and was shifted to lower temperatures with the increase in Cr3+ substitution. The leakage current density also reduced in magnitude with the increase in the Cr3+ substitution.
Degree Splitting of Root Square Mean Graphs  [PDF]
S. S. Sandhya, S. Somasundaram, S. Anusa
Applied Mathematics (AM) , 2015, DOI: 10.4236/am.2015.66086
Abstract: Let \"\" be an injective function. For a vertex labeling f, the induced edge labeling \"\" is defined by, \"\" or \"\"; then, the edge labels are distinct and are from \"\". Then f is called a root square mean labeling of G. In this paper, we prove root square mean labeling of some degree splitting graphs.
A Parameter Estimation Model of G-CSF: Mathematical Model of Cyclical Neutropenia  [PDF]
S. Balamuralitharan, S. Rajasekaran
American Journal of Computational Mathematics (AJCM) , 2012, DOI: 10.4236/ajcm.2012.21002
Abstract: We investigate the FFT (Fast Fourier Transform) model and G-CSF (granulocyte colony-stimulating factor) treatment of CN (Cyclical Neutropenia). We collect grey collies and normal dog’s data from CN and analyze the G-CSF treatment. The model develops the dynamics of circulating blood cells before and after the G-CSF treatment. This is quite natural and useful for the collection of laboratory data for investigation. The proposed interventions are practical. This reduces the quantity of G-CSF required for potential maintenance. This model gives us good result in treatment. The changes would be practical and reduce the risk side as well as the cost of treatment in G-CSF.
Synthesis, Thermal Behaviour, XRD, and Luminescent Properties of Lighter Lanthanidethiodipropionate Hydrates Containing Aminogunidine as Neutral Ligand  [PDF]
S. Packiaraj, S. Govindarajan
Open Journal of Inorganic Chemistry (OJIC) , 2014, DOI: 10.4236/ojic.2014.43006
Abstract:
Aminoguanidine lanthanide thiodipropionate hydrates of composition [Ln(Agun)2(tdp)3·nH2O], Agun = Aminoguanidine, tdp = thiodipropionic acid, where Ln = La, Pr, Nd and Sm if n = 2, have been prepared and characterized by physic-chemical techniques.
Fuzzy Based Intelligent Monitoring of Critical Lines in the Restructured Power Market  [PDF]
S. Rajasekaran, S. Sathiyamoorthy
Circuits and Systems (CS) , 2016, DOI: 10.4236/cs.2016.79191
Abstract: Restructured electric market environment allows the power wheeling transactions between the power producers and customers to meet the growing load demand. This will lead to the possible of congestion in the transmission lines. The possible contingencies of power components further worsen the scenario. This paper describes the methodology for the identification of critical transmission line by computing the real power and reactive power performance indices. It also demonstrates the importance of fuzzy logic technique used to rank the transmission lines according to the severity and demonstrated on IEEE-30 bus system.
Error Correction Circuit for Single-Event Hardening of Delay Locked Loops  [PDF]
S. Balaji, S. Ramasamy
Circuits and Systems (CS) , 2016, DOI: 10.4236/cs.2016.79210
Abstract: In scaled CMOS processes, the single-event effects generate missing output pulses in Delay-Locked Loop (DLL). Due to its effective sequence detection of the missing pulses in the proposed Error Correction Circuit (ECC) and its portability to be applied to any DLL type, the ECC mitigates the impact of single-event effects and completes its operation with less design complexity without any concern about losing the information. The ECC has been implemented in 180 nm CMOS process and measured the accuracy of mitigation on simulations at LETs up to 100 MeV-cm2/mg. The robustness and portability of the mitigation technique are validated through the results obtained by implementing proposed ECC in XilinxArtix 7 FPGA.
High Frequency Charging Techniques—Grid Connected Power Generation Using Switched Reluctance Generator  [PDF]
S. Sridharan, S. Sudha
Circuits and Systems (CS) , 2016, DOI: 10.4236/cs.2016.714352
Abstract: Power generation becomes the need of developed, developing and under developed countries to meet their increasing power requirements. When affordability increases their requirement of power increases, this happens when increased per capita consumption. The existing power scenario states that highest power is produced using firing of coals called thermal energy. A high efficiency Switched Reluctance Generator (SRG) based high frequency switching scheme to enhance the output for grid connectivity is designed, fabricated and evaluated. This proposed method generates the output for the low wind speed. It provides output at low speed because of multi-level DC-DC converter and storage system. It is an efficient solution for low wind power generation. The real time readings and results are discussed.
Calculation of the Gravitational Constant G Using Electromagnetic Parameters  [PDF]
Jesús Sánchez
Journal of High Energy Physics, Gravitation and Cosmology (JHEPGC) , 2017, DOI: 10.4236/jhepgc.2017.31012
Abstract: In this paper, we will derive the following formula for the value of the gravitational constant G: \"\" (1). This equation has only 0.81% error compared to the common accepted value \"\" [1]. The parameters in the equation are the following: \"\" the fine structure constant, q the elementary charge, \"\" the mass of the electron, \"\" the permittivity of the free space, e the exponential function and \"\" the relation between a circumference and its diameter. Values attached: \"\" [2], \"\"</span
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