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Search Results: 1 - 10 of 326808 matches for " S. Nath Sanyal "
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Evaluation of chemopreventive response of two cycloxygenase-2 inhibitors, etoricoxib and diclofenac in rat colon cancer using FTIR and NMR spectroscopic techniques
Kaur Saini,M.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2010,
Abstract: non steroidal anti inflammatory drugs (nsaids) are efficacious in chemoprevention of colorectal cancer. therefore, the potential ability of etoricoxib, a selective cycloxygenase-2(cox-2) inhibitor and diclofenac, a preferential cox-2 inhibitor are considered in the chemoprevention of 1, 2-dimethylhydrazine (dmh) induced colon carcinogenesis in rat model. dmh was injected s.c. for six weeks while etoricoxib and diclofenac were fed daily orally alone and also in combination with an weekly subcutaneous injection of 1,2-dimethylhydrazine dihydrochloride (dmh) to the rats. after the treatment period of 6 weeks the animals were sacrificed by an overdose of ether anesthesia and the colonic tissues were removed and studied by the ftir and nmr spectroscopic techniques to evaluate the changes occurring in the lipid bilayer of colonic membrane lipids. the alterations in wave number of ftir spectra as well as the chemical shifts of nmr spectra were recorded which signify the modulation of membrane lipids during colon carcinogenesis and possible cancer prevention by the oral administration of nsaids in an experimental model of chemical induced colon carcinogenesis.
Evaluation of chemopreventive response of two cycloxygenase-2 inhibitors, etoricoxib and diclofenac in rat colon cancer using FTIR and NMR spectroscopic techniques Evaluación de la respuesta quimiopreventiva de dos inhibidores de la ciclooxigenasa 2, etoricoxib y diclofenaco en el cáncer de colon murino empleando las técnicas espectroscópicas FTIR Y NMR
M. Kaur Saini,S. Nath Sanyal
Nutrición Hospitalaria , 2010,
Abstract: Non steroidal anti inflammatory drugs (NSAIDs) are efficacious in chemoprevention of colorectal cancer. Therefore, the potential ability of Etoricoxib, a selective cycloxygenase-2(COX-2) inhibitor and Diclofenac, a preferential COX-2 inhibitor are considered in the chemoprevention of 1, 2-dimethylhydrazine (DMH) induced colon carcinogenesis in rat model. DMH was injected s.c. for six weeks while Etoricoxib and Diclofenac were fed daily orally alone and also in combination with an weekly subcutaneous injection of 1,2-dimethylhydrazine dihydrochloride (DMH) to the rats. After the treatment period of 6 weeks the animals were sacrificed by an overdose of ether anesthesia and the colonic tissues were removed and studied by the FTIR and NMR Spectroscopic techniques to evaluate the changes occurring in the lipid bilayer of colonic membrane lipids. The alterations in wave number of FTIR spectra as well as the chemical shifts of NMR spectra were recorded which signify the modulation of membrane lipids during colon carcinogenesis and possible cancer prevention by the oral administration of NSAIDs in an experimental model of chemical induced colon carcinogenesis. Los fármacos antiinflamatorios no esteroideos (AINE) son eficaces en la prevención del cáncer colorrectal. Por lo tanto, la capacidad potencial de Etoricoxib, un inhibidor selectivo de la ciclooxigenasa-2(COX-2), y de Diclofenaco, un inhibidor preferencial de la COX-2, se considera en la quimioprevención de la carcinogénesis de colon inducida por 1, 2-dimetilhidracina (DMH) en el modelo murino. Se inyectó s.c. DMH durante 6 semanas a la vez que se administraban diariamente por vía oral Etoricoxib y Diclofenaco solos y en combinación con una inyección s.c. semanal de dihidrocloruro de 1,2-dimetilhidracina (DMH) a las ratas. Después del período de tratamiento de 6 semanas, se sacrificó a los animales mediante una sobredosis de anestesia con éter y se extirpó el tejido colónico para estudio con las técnicas espectroscópicas FTIR y NMR para evaluar los cambios que ocurrieron en la bicapa lipídica de las membranas lipídicas colónicas. Se registraron las alteraciones en el número de onda del espectro FTIR así como las desviaciones químicas del espectro NMR, lo que significa la modulación de los lípidos de membrana durante la carcinogénesis colónica y la posible prevención del cáncer mediante la administración de AINE por vía oral en un modelo experimental de carcinogénesis colónica inducida por un agente químico.
Effect of non-steroidal anti-inflammatory drugs and the pro-carcinogen 1, 2 dimethylhydrazine on the rat intestinal membrane structure and function
Mittal,N.; Singh Kanwar,S.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2008,
Abstract: the present study was designed to evaluate the effects of three non-steroidal anti-inflammatory drugs (nsaids) with varying cycloxygenase selectivities on the small intestinal biochemical composition, function and histology during 1, 2-dimethylhydrazine (dmh) administration. sprague dawley male rats were divided into five different groups viz: group 1 (control, vehicle treated), group 2 (dmh-treated, 30 mg/kg body weight/week in 1 mm edta-saline, subcutaneously), group 3 (dmh + aspirin-60 mg/kg body weight), group 4 (dmh + celecoxib-6 mg/kg body weight), group 5 (dmh + etoricoxib-0.64 mg/kg body weight). after six weeks of treatment, brush border membrane was isolated from the jejunum segment of all the groups and changes in the associated enzymes such as sucrase, lactase, maltase, alkaline phosphatase, membrane lipid composition, fluorescence polarizations of diphenylhexatriene, pyrene excimer formation, histological changes and surface characteristics were studied. the results indicated a significant alteration in the enzyme activity as well as changes in the structure and function of the intestine in the presence of the pro-carcinogen, dmh, which suggests the possible chemopreventive efficacy of nsaids against the intestinal cancer.
Intestinal toxicity of non-steroideal anti-inflammatory drugs with differential cyclooxigenase inhibition selectivity
Chopra,S.; Kishore Saini,R.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2007,
Abstract: the present study was designed to investigate the gastrointestinal side effects of cycloxygenase (cox) inhibitor with varying selectivity, called the non-steroidal antiinflammatory drugs (nsaids) viz., non-selective cox-1 & 2 inhibitor -aspirin, prefentially selective cox-2 inhibitor- nimesulide and highly selective cox-2 inhibitor- celecoxib. treatment with nsaids exhibited a decrease in the activity of rat intestinal brush border membrane associated enzymes such as sucrase, lactase, maltase and alkaline phosphatase as compared to the control in the duodenum, jejunum and ileum. the uptake of d-glucose and l-histidine in the everted intestinal sac was found to be decreased. also the decease of glucose and histidine uptake was found to be dependent on the substrate concentration, temperature and the time interval of incubation. the physical state and composition of brush border membrane was found to be altered as evident in the ftir spectrum, by appearance of new peaks while disappearance of certain peaks occurred which were characteristics of the control membrane. the changes in wave number as well as peaks height were also noticed. alterations in protein profile of the membrane were demonstrated using sds-page analysis where disappearance of few bands and change in the relative intensities of the bands were noticed and correlated with the alterations that have taken place at the molecular level. histological studies have depicted a marked decrease in the absorption surface area such as the villi height of the intestinal segment. in addition, crypt number also deceased in the treated animals, an indication that such changes also correlate well with the changes in the transport of the end product nutrients.
Effects of non steroidal anti-inflammatory drugs on the antioxidant defense system and the membrane functions in the rat intestine
Nair,P.; Singh Kanwar,S.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2006,
Abstract: in the present study the effects of two cycloxygenase-2 (cox-2) selective inhibitors, celecoxib and nimesulide as compared to a non-selective cox inhibitor, aspirin was studied in the rat intestine. female wistar rats weighing between 150-175 g were divided into four groups having 8 animals each as follows: group 1(control), group 2- aspirin (40 mg/kg), group 3- nimesulide (10 mg/kg) and group 4- celecoxib (10 mg/kg). after 35 days of treatment the animals were sacrificed, intestine removed and the effects on the antioxidant defense system, membrane composition and functions along with the membrane specific enzymes were studied in different regions of the intestine. the study showed a significant increase in the lipid peroxide levels as tba-reactive substance as well as the conjugated dienes, except for celecoxib treated group which showed a decrease. significant decrease was also observed in the level of reduced glutathione (gsh), superoxide dismutase (sod), glutathione-s-transferase and catalase activities for aspirin and nimesulide group while celecoxib caused an increase in glutathione reductase (gr). aspirin and nimesulide exhibited an increase in the brush border membrane (bbm) bound enzyme activities like sucrase, lactase, maltase and alkaline phosphatase in the small intestine while celecoxib showed decrease in lactase, maltase and alkaline phosphatase. the phospholipid content increased only for aspirin treated group while cholesterol decreased in all the treatment groups. also celecoxib treatment brought about an increase in glycolipid content. the membrane fluidity was studied by the rotational diffusion of 1, 6, diphenyl, 1, 3, 5 hexatriene (dph) incorporated in the membrane and the fluorescence polarization (p), fluorescence anisotropy(r), anisotropy parameter [r0/r -1]-1 and order parameter [s2 = (4/3r - 0.1)/ r0] were recorded. no significant change in the fluorescence parameters were observed in the bbm and the liposomes made from the bbm lipids for
Intestinal toxicity of non-steroideal anti-inflammatory drugs with differential cyclooxigenase inhibition selectivity Toxicidad intestinal de los fármacos antiinflamatorios no esteroideos con una selectividad diferenciada en la inhibición de la ciclooxigenasa
S. Chopra,R. Kishore Saini,S. Nath Sanyal
Nutrición Hospitalaria , 2007,
Abstract: The present study was designed to investigate the gastrointestinal side effects of cycloxygenase (COX) inhibitor with varying selectivity, called the non-steroidal antiinflammatory drugs (NSAIDs) viz., non-selective COX-1 & 2 inhibitor -aspirin, prefentially selective COX-2 inhibitor- nimesulide and highly selective COX-2 inhibitor- celecoxib. Treatment with NSAIDs exhibited a decrease in the activity of rat intestinal brush border membrane associated enzymes such as sucrase, lactase, maltase and alkaline phosphatase as compared to the control in the duodenum, jejunum and ileum. The uptake of D-glucose and L-histidine in the everted intestinal sac was found to be decreased. Also the decease of glucose and histidine uptake was found to be dependent on the substrate concentration, temperature and the time interval of incubation. The physical state and composition of brush border membrane was found to be altered as evident in the FTIR spectrum, by appearance of new peaks while disappearance of certain peaks occurred which were characteristics of the control membrane. The changes in wave number as well as peaks height were also noticed. Alterations in protein profile of the membrane were demonstrated using SDS-PAGE analysis where disappearance of few bands and change in the relative intensities of the bands were noticed and correlated with the alterations that have taken place at the molecular level. Histological studies have depicted a marked decrease in the absorption surface area such as the villi height of the intestinal segment. In addition, crypt number also deceased in the treated animals, an indication that such changes also correlate well with the changes in the transport of the end product nutrients. Se dise ó este estudio para investigar los efectos adversos gastrointestinales de los inhibidores de la ciclooxigenasa (COX) con selectividad variable, denominados fármacos antiinflamatorios no esteroideos (AINE), inhibidores no selectivos de la COX-1 y la COX-2 -aspirina, los inhibidores predominantemente selectivos de la COX-2- nimesulida, y los inhibidores muy selectivos de la COX-2 -celecoxib. El tratamiento con AINE mostró un descenso de la actividad de las enzimas asociadas a la membrana en cepillo intestinal de la rata, tales como sucrasa, lactasa, maltasa y fosfatasa alcalina, en comparación con el control, en el duodeno, yeyuno e íleon. Se halló que la captación de D-glucosa y L-histidina en el saco intestinal revertido estaba disminuida. También se encontró que la captación de glucosa e histidina dependía de la concentración de sustrato, la t
Effects of non steroidal anti-inflammatory drugs on the antioxidant defense system and the membrane functions in the rat intestine Efectos de los fármacos aniinflamatorios no esteroideos sobre el sistema de defensa antioxidante y las funciones de membrana en el intestino de rata
P. Nair,S. Singh Kanwar,S. Nath Sanyal
Nutrición Hospitalaria , 2006,
Abstract: In the present study the effects of two cycloxygenase-2 (COX-2) selective inhibitors, celecoxib and nimesulide as compared to a non-selective COX inhibitor, aspirin was studied in the rat intestine. Female Wistar rats weighing between 150-175 g were divided into four groups having 8 animals each as follows: Group 1(Control), Group 2- Aspirin (40 mg/kg), Group 3- Nimesulide (10 mg/kg) and Group 4- Celecoxib (10 mg/kg). After 35 days of treatment the animals were sacrificed, intestine removed and the effects on the antioxidant defense system, membrane composition and functions along with the membrane specific enzymes were studied in different regions of the intestine. The study showed a significant increase in the lipid peroxide levels as TBA-reactive substance as well as the conjugated dienes, except for celecoxib treated group which showed a decrease. Significant decrease was also observed in the level of reduced glutathione (GSH), superoxide dismutase (SOD), glutathione-s-transferase and catalase activities for aspirin and nimesulide group while Celecoxib caused an increase in glutathione reductase (GR). Aspirin and nimesulide exhibited an increase in the brush border membrane (BBM) bound enzyme activities like sucrase, lactase, maltase and alkaline phosphatase in the small intestine while celecoxib showed decrease in lactase, maltase and alkaline phosphatase. The phospholipid content increased only for aspirin treated group while cholesterol decreased in all the treatment groups. Also celecoxib treatment brought about an increase in glycolipid content. The membrane fluidity was studied by the rotational diffusion of 1, 6, diphenyl, 1, 3, 5 hexatriene (DPH) incorporated in the membrane and the fluorescence polarization (p), fluorescence anisotropy(r), anisotropy parameter [r0/r -1]-1 and order parameter [S2 = (4/3r - 0.1)/ r0] were recorded. No significant change in the fluorescence parameters were observed in the BBM and the liposomes made from the BBM lipids for the treatment groups. These results indicate that celecoxib may be accepted as a safer drug in terms of overall gastro-intestinal toxicity as compared to the aspirin and nimesulide. En el presente estudio se comparaban los efectos de dos inhibidores selectivos de la ciclo-oxigenasa-2 (COX-2), celecoxib y nimesulide, con los de un inhibidor no selectivo de la COX, la aspirina, en el intestino de la rata. Se escogieron ratas Wistar hembra, con un peso de 150-175 g, y se las dividió en 4 grupos, con 8 animales cada uno, como sigue: Grupo 1 (Control), Grupo 2 - Aspirina (40 mg/kg), Grupo 3 - Nimes
Effect of non-steroidal anti-inflammatory drugs and the pro-carcinogen 1, 2 dimethylhydrazine on the rat intestinal membrane structure and function Efecto de los fármacos antiinflmatorios no esteroideos y del procarcinógeno 1,2-dimetilhidracina sobre la estructura y función de la membrana intestinal de la rata
N. Mittal,S. Singh Kanwar,S. Nath Sanyal
Nutrición Hospitalaria , 2008,
Abstract: The present study was designed to evaluate the effects of three non-steroidal anti-inflammatory drugs (NSAIDs) with varying cycloxygenase selectivities on the small intestinal biochemical composition, function and histology during 1, 2-dimethylhydrazine (DMH) administration. Sprague Dawley male rats were divided into five different groups viz: Group 1 (control, vehicle treated), Group 2 (DMH-treated, 30 mg/kg body weight/week in 1 mM EDTA-saline, subcutaneously), Group 3 (DMH + aspirin-60 mg/kg body weight), Group 4 (DMH + celecoxib-6 mg/kg body weight), Group 5 (DMH + etoricoxib-0.64 mg/kg body weight). After six weeks of treatment, brush border membrane was isolated from the jejunum segment of all the groups and changes in the associated enzymes such as sucrase, lactase, maltase, alkaline phosphatase, membrane lipid composition, fluorescence polarizations of diphenylhexatriene, pyrene excimer formation, histological changes and surface characteristics were studied. The results indicated a significant alteration in the enzyme activity as well as changes in the structure and function of the intestine in the presence of the pro-carcinogen, DMH, which suggests the possible chemopreventive efficacy of NSAIDs against the intestinal cancer. El presente estudio se dise ó para evaluar los efectos de tres fármacos antiinflamatorios no esteroideos (AINE) con diferente selectividad por la ciclooxigenasa sobre la composición bioquímica, la función y la histología del intestino delgado durante la administración de 1,2-dimetilhidracina (DMH). Se distribuyó a ratas macho Sprague Dawley en grupos distintos: Grupo 1 (control, tratado con vehículo), Grupo 2 (tratado con DMH, 30 mg/kg de peso /semana en 1 mM de EDTA-salino, subcutáneo), Grupo 3 (DMH + aspirina-60 mg/kg de peso), Grupo 4 (DMH + celecoxib-6 mg/kg de peso), Grupo 5 (DMH + etoricoxib-0,64 mg/kg de peso). Tras seis semanas de tratamiento, se aisló la membrana en cepillo de un segmento del yeyuno en todos los grupos y se estudiaron los cambios en las enzimas asociadas tales como sucrasa, lactasa, maltasa, fosfatasa alcalina, en la composición lipídica de la membrana, las polarizaciones de fluorescencia del difenilhexatrieno, la formación del excímero pireno, los cambios histológicos y las características de la superficie. Los resultados indican una alteración significativa de la actividad enzimática así como cambios en la estructura y función del intestino en presencia del procarcinógeno DMH, lo que sugiere la posible eficacia quimio-preventiva de los AINE frente al cáncer de intestino.
The effect of prostaglandin synthase inhibitor, aspirin on the rat intestinal membrane structure and function
Kaur,G.; Kaur,J.; Mittal,N.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2010,
Abstract: aspirin at a dose of 50 mg/kg body weight was found to decrease the activity of the rat intestinal brush border membrane (bbm) - associated enzymes such as the sucrase, lactase, maltase and alkaline phosphatase. aspirin treatment also led to a decrease in the microviscosity in the native as well as the benzyl alcohol treated membrane which might be due to the lipid peroxidative damage in the membrane. physical correlation of the membrane oxidative damage was evident as the fourier transformation infra red (ftir) study of the aspirin treated membrane, which include an increased proportion of gauche to trans conformer, shift in the methylene c-h asymmetric and symmetric stretching frequencies, c = o double bond stretching, nh bending, antisymmetric (n)-ch3 bending, c-n stretching and antisymmetric cnc stretching while there was no change in the ch2 wagging and twisting as well as in nh-bending amide bond i and ii. aspirin treatment also caused an alteration in the glucose and histidine transport, as evident by a decreased vmax value while the apparent km remaining unchanged in the control and aspirin-treated animals confirming that there was no change in the substrate affinity constant of the membrane transport proteins for the glucose and the basic amino acid, although the rate of transport decreased considerably. there was a decrease noted in the energy of activation of glucose and histidine transport when studied at different temperature but no change in the temperature of phase transition in the bbm with aspirin treatment, thus implying that perhaps the thermotropic phase transition in the membrane may have relatively little effect on the transport processes. the result suggests an underlying molecular mechanism indicating the implied membrane damage by aspirin, an important member of the non-steroidal antiinflammatory drug (nsaid) family which could possibly through an oxidative damage may lead to an altered molecular structure, physical state and biological func
Chemopreventive response of diclofenac, a non-steroidal anti-inflammatory drug in experimental carcinogenesis
Kaur Saini,M.; Kaur,J.; Sharma,P.; Nath Sanyal,S.;
Nutrición Hospitalaria , 2009,
Abstract: the chemopreventive response was evaluated of nonsteroidal anti-inflammatory drug, diclofenac, a preferential cyclooxygenase-2 (cox-2) inhibitor in 1,2-dimethyhydrazine (dmh)-induced colon cancer in rat model. the signs of neoplasm were evident in the animals receiving 30mg of dmh per kg body weight in a weekly s.c injection for six weeks. the putative biomarker of carcinogenesis was visible in the form of multiple plaque lesions in dmh treatment and then regression seen in those animals which also received an oral dose of diclofenac, 8 mg/kg body weight whereas no such macroscopic neoplastic lesions were seen in the animals receiving diclofenac only or the control animals receiving the vehicle of the drug. histopathological results showed the presence of early aberrant changes in the form of severe dysplasia and also numerous crypt fissions in the apical surface of the colonic mucosa. a very high expression of cox-2 was seen in the colonic epithelium of dmh-treated rats, as analyzed by immunohistochemistry. also, the apoptotic events were assessed by terminal deoxynucleotidyl dutp nick end labeling (tunel) assay, where the dmh group shows few number of tunel positive cells which dramatically increased in the diclofenac treatment. the results suggest that diclofenac could be an effective chemopreventive agent in colon cancer, where perhaps apoptosis plays a very dominant end effect in cancer cell killings.
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