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Search Results: 1 - 10 of 673282 matches for " S. A. Kotova "
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Low molecular weight thymic factor inhibits histamine release from basophils
S. I. Yalkut,E. V. Gulling,T. Yu. Gots,S. A. Kotova
Mediators of Inflammation , 1993, DOI: 10.1155/s0962935193000638
DNA fragments binding CTCF in vitro and in vivo are capable of blocking enhancer activity
Dmitry A Didych, Elena S Kotova, Segey B Akopov, Lev G Nikolaev, Eugene D Sverdlov
BMC Research Notes , 2012, DOI: 10.1186/1756-0500-5-178
Abstract: Ten CTCF-binding DNA fragments were inserted between the CMV enhancer and CMV minimal promoter driving the herpes simplex virus thymidine kinase (HSV-tk) gene in a vector expressing also the neoR gene under a separate promoter. The constructs were then integrated into the genome of CHO cells, and the cells resistant to neomycin and ganciclovir (positive-negative selection) were picked up, and their DNAs were PCR analyzed to confirm the presence of the fragments between the enhancer and promoter in both orientations.We demonstrated that all sequences identified by their CTCF binding both in vitro and in vivo had enhancer-blocking activity when inserted between the CMV minimal promoter and enhancer in stably transfected CHO cells.Spatial, temporal and tissue specific gene expression in mammals is largely determined by genomic cis-regulatory elements, such as promoters, enhancers, silencers, and insulators (for recent review, see [1,2]). A survey of about 1% of the human genome [3] indicated that the regulatory elements were more abundant in the genome than the genes they control and are mostly distal to the genes that they regulate.While the number and positions of enhancer elements in the whole human genome can be determined with some certainty through P300 binding [4], the number and positions of most insulator elements are not known [2], and methods of their identification in mammals are sparse. Moreover, the definition of insulator is somewhat ambiguous--this term designates elements with enhancer-blocking or chromatin-bordering functions (reviewed in [5]) which are not interrelated at least in some cases [6,7]. In addition, the term "insulator" is sometimes used to designate the elements that bind the CTCF protein but have no proven enhancer-blocking or chromatin-bordering activity.Two basic approaches have been proposed to identify many potential genomic insulators in one experiment. One approach is based on the ChIP-on-chip or ChIP-seq techniques with antibodie
A.A. Kotova
Voprosy Sovremennoj èkonomiki , 2013,
Abstract: The article presents the trends in the Russian financial market and the conditions for integration into the global financial system. Main directions of integration of the financial market of Russia into the global financial system, such as: reform of the existing model of the financial market in Russia with respect to evade speculative model integration and development with developing countries, countries of CIS and BRIC. Stimulation of real investment in the Russian economy. Regular monitoring of investment imbalances in the framework of the state investment policy with regard to determine the excess or deficit of the necessary investment capital by comparing the maximum amount of available internal resources and investment needs within the planned period.
A.A. Kotova
Voprosy Sovremennoj èkonomiki , 2013,
Abstract: The article presents a model of international financial integration, shows the advantages and disadvantages of integration of financial markets, identified the benefits and potential risks of the penetration of foreign banks in the financial markets.
Geomaterials on the Basis of Analcime-Bearing Rocks  [PDF]
Dmitry A. Shushkov, Olga B. Kotova, Boris A. Goldin
Geomaterials (GM) , 2011, DOI: 10.4236/gm.2011.12006
Abstract: Analcime-bearing rocks (Komi Republic, Russia) are perspective raw for geological materials. The article represents possibilities of their application for water purification, as raw for ceramic industry and poor ore for aluminum industry.
Reactive Chemical Vapor Deposition Method as New Approach for Obtaining Electroluminescent Thin Film Materials
Valentina V. Utochnikova,Oxana V. Kotova,Andrey A. Vaschenko,Leonid S. Lepnev,Alexei G. Vitukhnovsky,Natalia P. Kuzmina
Advances in Materials Science and Engineering , 2012, DOI: 10.1155/2012/809028
Abstract: The new reactive chemical vapor deposition (RCVD) method has been proposed for thin film deposition of luminescent nonvolatile lanthanide aromatic carboxylates. This method is based on metathesis reaction between the vapors of volatile lanthanide dipivaloylmethanate (Ln(dpm)3) and carboxylic acid (HCarb orH2Carb′) and was successfully used in case of HCarb. Advantages of the method were demonstrated on example of terbium benzoate (Tb(bz)3) and o-phenoxybenzoate thin films, and Tb(bz)3 thin films were successfully examined in the OLED with the following structure glass/ITO/PEDOT:PSS/TPD/Tb(bz)3/Ca/Al. Electroluminescence spectra of Tb(bz)3 showed only typical luminescent bands, originated from transitions of the terbium ion. Method peculiarities for deposition of compounds of dibasic acids H2Carb′ are established on example of terbium and europium terephtalates and europium 2,6-naphtalenedicarboxylate. 1. Introduction A search for new electroluminescent (EL) materials for organic light-emitting diodes (OLEDs) is still an actual challenge for chemists. The solution of it means not only finding a compound which fits the EL material requirements but also the right choice or creation of a technique providing the transformation of such precursors into the thin films of high smoothness and low thickness. This can be demonstrated on examples of lanthanide coordination compounds which are well-known potential luminescent materials [1–5]. However, it is not that simple to find among them the luminescent lanthanide coordination compound, which demonstrates simultaneously high thermal and UV stability, bright luminescence, while being volatile or possesses high solubility to deposit thin film of high quality by physical methods from gas phase or solutions. In case of highly volatile β-diketonates the obvious physical deposition technique allows to obtain thin films of high quality, and their luminescence efficiency can be increased by variation of the substituents in ligands. However these compounds do not exhibit required UV stability. A different situation arises from lanthanide aromatic carboxylates which show high UV, thermal, and chemical stability, as well as high luminescence efficiency, which can reach 100% in case of Tb(bz)3 [6–9], but for these compounds the choice for the film deposition technique among known methods appears rather impossible. The aromatic carboxylates form extremely rigid 3D networks [10–12], which make them nonvolatile and poorly soluble in organic solvents that complicate deposition of their thin films from gas phase or solution and
Investigation of distortions of the chirp signals in the neighborhood of critical frequencies of ionospheric plasma
Zakharov V. E.,Kotova D. S.
Vestnik Immanuel Kant Baltic Federal University , 2013,
Abstract: Numerical experiments are performed to investigate the dispersion distortions of chirp pulses propagating in the ionosphere with taking the three-dimensional non-uniformity, anisotropy, and dispersion of ionosphere in dependence on geophysical conditions into account. Propagation, absorption,and the time delay of ordinary and nonordinary waves are analased.
Polypeptide Modulators of TRPV1 Produce Analgesia without Hyperthermia
Yaroslav A. Andreev,Sergey A. Kozlov,Yuliya V. Korolkova,Igor A. Dyachenko,Dmitrii A. Bondarenko,Denis I. Skobtsov,Arkadii N. Murashev,Polina D. Kotova,Olga A. Rogachevskaja,Natalia V. Kabanova,Stanislav S. Kolesnikov,Eugene V. Grishin
Marine Drugs , 2013, DOI: 10.3390/md11125100
Abstract: Transient receptor potential vanilloid 1 receptors (TRPV1) play a significant physiological role. The study of novel TRPV1 agonists and antagonists is essential. Here, we report on the characterization of polypeptide antagonists of TRPV1 based on in vitro and in vivo experiments. We evaluated the ability of APHC1 and APHC3 to inhibit TRPV1 using the whole-cell patch clamp approach and single cell Ca 2+ imaging. In vivo tests were performed to assess the biological effects of APHC1 and APHC3 on temperature sensation, inflammation and core body temperature. In the electrophysiological study, both polypeptides partially blocked the capsaicin-induced response of TRPV1, but only APHC3 inhibited acid-induced (pH 5.5) activation of the receptor. APHC1 and APHC3 showed significant antinociceptive and analgesic activity in vivo at reasonable doses (0.01–0.1 mg/kg) and did not cause hyperthermia. Intravenous administration of these polypeptides prolonged hot-plate latency, blocked capsaicin- and formalin-induced behavior, reversed CFA-induced hyperalgesia and produced hypothermia. Notably, APHC3’s ability to inhibit the low pH-induced activation of TRPV1 resulted in a reduced behavioural response in the acetic acid-induced writhing test, whereas APHC1 was much less effective. The polypeptides APHC1 and APHC3 could be referred to as a new class of TRPV1 modulators that produce a significant analgesic effect without hyperthermia.
Penetrating Cations Enhance Uncoupling Activity of Anionic Protonophores in Mitochondria
Yuri N. Antonenko, Ljudmila S. Khailova, Dmitry A. Knorre, Olga V. Markova, Tatyana I. Rokitskaya, Tatyana M. Ilyasova, Inna I. Severina, Elena A. Kotova, Yulia E. Karavaeva, Anastasia S. Prikhodko, Fedor F. Severin, Vladimir P. Skulachev
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0061902
Abstract: Protonophorous uncouplers causing a partial decrease in mitochondrial membrane potential are promising candidates for therapeutic applications. Here we showed that hydrophobic penetrating cations specifically targeted to mitochondria in a membrane potential-driven fashion increased proton-translocating activity of the anionic uncouplers 2,4-dinitrophenol (DNP) and carbonylcyanide-p-trifluorophenylhydrazone (FCCP). In planar bilayer lipid membranes (BLM) separating two compartments with different pH values, DNP-mediated diffusion potential of H+ ions was enhanced in the presence of dodecyltriphenylphosphonium cation (C12TPP). The mitochondria-targeted penetrating cations strongly increased DNP- and carbonylcyanide m-chlorophenylhydrazone (CCCP)-mediated steady-state current through BLM when a transmembrane electrical potential difference was applied. Carboxyfluorescein efflux from liposomes initiated by the plastoquinone-containing penetrating cation SkQ1 was inhibited by both DNP and FCCP. Formation of complexes between the cation and CCCP was observed spectophotometrically. In contrast to the less hydrophobic tetraphenylphosphonium cation (TPP), SkQ1 and C12TPP promoted the uncoupling action of DNP and FCCP on isolated mitochondria. C12TPP and FCCP exhibited a synergistic effect decreasing the membrane potential of mitochondria in yeast cells. The stimulating action of penetrating cations on the protonophore-mediated uncoupling is assumed to be useful for medical applications of low (non-toxic) concentrations of protonophores.
CONTROL OF AKABANE VIRUS PERSISTENCE IN A VERO CELL CULTURE USING THE DIRECT IMMUNOFLUORESCENCE METHOD Контроль персистенции вируса болезни Акабане в культуре клеток VERO с помощью прямого метода иммунофлюоресценции
Kotova O. Y.,Khan E. O.,Kushnir S. D.,Ponomarev V. N.
Polythematic Online Scientific Journal of Kuban State Agrarian University , 2012,
Abstract: The article covers the Akabane disease agent identification in the new green monkey kidney continuous cell line (VERO) that has not been used for that in Russia before. Besides, the time for the virus detection using the fluorescent antibody method was found to be considerable decreased
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