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Search Results: 1 - 10 of 329771 matches for " Sílvia Leite "
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Carta ao Editor
Sílvia Leite,José Cotter
Jornal Português de Gastrenterologia , 2009,
A constru??o coletiva com crian?as em ambientes virtuais de aprendizagem
Leite, Sílvia Meirelles;Behar, Patricia Alejandra;
Educar em Revista , 2007, DOI: 10.1590/S0104-40602007000100012
Abstract: this article presents an experience with children in relation to the collectivity, in which the effects of the exchanges was accompanied among the subjects. based on this, it is presented an investigation about the interindividual interaction process and the meaning production in a virtual learning environments, aiming the knowledge construction. therefore, it was used the crianet software platform and the etc's collective text publisher as digital supports for the constitution of directed virtual environments to the collective and the learning process. the reading of that process contributed to the understanding of the infantile public insert in the internet, consolidating it while a way of communication that benefice the tranverse and the reciprocity.
Hybridization-Based Detection of Helicobacter pylori at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes
Sílvia Fontenete, Nuno Guimar?es, Marina Leite, Céu Figueiredo, Jesper Wengel, Nuno Filipe Azevedo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0081230
Abstract: The understanding of the human microbiome and its influence upon human life has long been a subject of study. Hence, methods that allow the direct detection and visualization of microorganisms and microbial consortia (e.g. biofilms) within the human body would be invaluable. In here, we assessed the possibility of developing a variant of fluorescence in situ hybridization (FISH), named fluorescence in vivo hybridization (FIVH), for the detection of Helicobacter pylori. Using oligonucleotide variations comprising locked nucleic acids (LNA) and 2’-O-methyl RNAs (2’OMe) with two types of backbone linkages (phosphate or phosphorothioate), we were able to successfully identify two probes that hybridize at 37 °C with high specificity and sensitivity for H. pylori, both in pure cultures and in gastric biopsies. Furthermore, the use of this type of probes implied that toxic compounds typically used in FISH were either found to be unnecessary or could be replaced by a non-toxic substitute. We show here for the first time that the use of advanced LNA probes in FIVH conditions provides an accurate, simple and fast method for H.?pylori detection and location, which could be used in the future for potential in vivo applications either for this microorganism or for others.
Literacy, Ethnicity and Style  [PDF]
Maria Sílvia Cintra
Advances in Anthropology (AA) , 2013, DOI: 10.4236/aa.2013.34A002

I present the result of a two-year research project developed at the “Instituto de Estudos de Linguagem” (IEL/Unicamp, Brazil) and also of action research I have been organizing since 2006 when I started to work as a professor at the “Universidade Federal de Sao Carlos”, in Brazil. Relying on recent developments in New Literacy Studies, I explore the concept of the continuum illiterate-literate and argue that it implies elements of transformation, as well as conservation. I also argue that three intersecting continua must be considered together: the continua oral-written, rural-urban and restricted-full literacy, always taking into consideration ethnicity as a variable (Cohen, 1974). Drawing on ethnographic fieldwork in Brazil, I show how elements of restricted literacy (Goody, 1968) are presented on the threshold of the twenty first century; how they entertain relation with the rural to urban migration and with a marked contrast between different ethnicities; and in what sense this fact may be visible in the everyday use of language and in the style inherent in it.

Nheengatu Language and Its Role in the Tactics of Construction of Indigenous Identity  [PDF]
Maria Sílvia Cintra
Advances in Anthropology (AA) , 2018, DOI: 10.4236/aa.2018.84012
Abstract: Nheengatu is one of the co-official languages of São Gabriel da Cachoeira in the Amazon region (AM/Brazil). About 8000 people in the Upper Rio Negro region speak it, and there exists a contemporary movement for its revitalization in the state of Pará (PA/Brazil). Based upon field research in both these regions, this paper bears reflections on the inter-relations existing between language and identity including references in the areas of Applied Linguistics, Culture Semiotics and Anthropology. I propose a discussion on Nheengatu language, which is often considered in a dysphoric way, i.e., as if it had been imposed on the natives, resulting only from the colonizers’ strategies. I propose we can instead envisage it from the perspective concerning the tactics constructed by the aboriginals in order to preserve and reconquer their identity, i.e., from the point of view of a Poetics of Relation within a complex, contradictory and hybrid linguistic approach.
Condi es de constru o histórica do sindicalismo docente de educa o básica
lvia Leite Cruz
Sociedade e Estado , 2008, DOI: 10.1590/s0102-69922008000100014
Neoplastic Transformation of T Lymphocytes through Transgenic Expression of a Virus Host Modification Protein
Sílvia Cristina Paiva Almeida, Vivian Leite de Oliveira, Sónia Ventura, Margarita Bofill, Robert Michael Evans Parkhouse
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034140
Abstract: Virus host evasion genes are ready-made tools for gene manipulation and therapy. In this work we have assessed the impact in vivo of the evasion gene A238L of the African Swine Fever Virus, a gene which inhibits transcription mediated by both NF-κB and NFAT. The A238L gene has been selectively expressed in mouse T lymphocytes using tissue specific promoter, enhancer and locus control region sequences for CD2. The resulting two independently derived transgenic mice expressed the transgene and developed a metastasic, angiogenic and transplantable CD4+CD8+CD69– lymphoma. The CD4+CD8+CD69– cells also grew vigorously in vitro. The absence of CD69 from the tumour cells suggests that they were derived from T cells at a stage prior to positive selection. In contrast, transgenic mice similarly expressing a mutant A238L, solely inhibiting transcription mediated by NF-κB, were indistinguishable from wild type mice. Expression of Rag1, Rag2, TCRβ-V8.2, CD25, FoxP3, Bcl3, Bcl2 l14, Myc, IL-2, NFAT1 and Itk, by purified CD4+CD8+CD69– thymocytes from A238L transgenic mice was consistent with the phenotype. Similarly evaluated expression profiles of CD4+CD8+ CD69– thymocytes from the mutant A238L transgenic mice were comparable to those of wild type mice. These features, together with the demonstration of (mono-)oligoclonality, suggest a transgene-NFAT-dependent transformation yielding a lymphoma with a phenotype reminiscent of some acute lymphoblastic lymphomas.
Doen?a de Bowen na regi?o perianal tratada com criocirurgia com nitrogênio líquido
Moraes, Aparecida Machado de;Leite, Sílvia Helena Rodrigues;Cintra, Maria Letícia;Terrazas, Eliane Ramires;Souza, Elemir Macedo de;
Anais Brasileiros de Dermatologia , 2002, DOI: 10.1590/S0365-05962002000500007
Abstract: bowen's disease is generally an asymptomatic in situ carcinoma that develops slowly. it rarely occurs in the genital region and mainly affects young persons. skin lesions occurring in areas not exposed to sunlight may be associated with other factors, like hpv, inflammatory disease or colon neoplasia. the disease may be treated by conventional surgery, cryosurgery or photodynamic therapy. the aim is to present the clinical characteristics of a rare case of bowen's disease in a young, black female, who had a large lesion in the perianal region, which tested positive for hpv. the woman had been undergoing treatment for intestinal ulcerative rectocolitis or crohn's disease. she was treated with cryosurgery and there was no sign of recurrence during the ensuing 34 months.
Caracteriza??o das proteínas de superfície de membrana externa da sorovariedade Hardjo isolada de bovinos em Minas Gerais
Rodrigues, Rogério O.;Silva, José A.;Alves, Telma M.;Dorneles, Elaine M.S.;Minharro, Sílvia;Lage, Andrey P.;Leite, R?mulo C.;Moreira, élvio C.;
Pesquisa Veterinária Brasileira , 2011, DOI: 10.1590/S0100-736X2011000700002
Abstract: differences in the protein profile of leptospira sp. strains sponselee, norma and hardjoprajitno were observed, with bands ranging from 175.47 kda to 12.10 kda. strain sponselee presented a 12-band profile, while strain norma showed 11 and strain hardjoprajitno showed 9 bands in the profile. all bands observed in sponselee strain profile could match bands in the other two strains. strain norma lacks a band at 35.77 kda and strain hardjoprajitno lacks the bands at 89.59 kda, 35.77 kda and 12.10 kda. the recognition profile from hyperimmune sera was also different for the studied serovar hadjo strains. the majority of recognized proteins was in the range of 35.83 kda to 29.19 kda. cattle sera against strain norma only recognized low molecular mass proteins in strains norma (6.80 kda) and hardjoprajitno (6.80 kda and 5.30 kda). bovine sera against strain hardjoprajitno recognized a 44.33 kda protein in all studied strains and proteins of 4.22 kda in strains sponselee and norma and of 10.49 kda and 6.16 kda in strain hadjoprajitno. the different identified proteins could become specific targets to the development of diagnostic tests and vaccines against bovine leptospirosis.
Cateterismo cardíaco esquerdo: lacunas nas informa??es transmitidas aos pacientes
Cavalcanti, Taciana de Castilhos;Sarmento-Leite, Rogério;Gottschall, Carlos A. M.;Quadros, Alexandre Schaan de;Goldmeier, Sílvia;Souza, Emiliane Nogueira de;Moraes, Maria Antonieta Pereira;
Revista Brasileira de Cardiologia Invasiva , 2008, DOI: 10.1590/S2179-83972008000200015
Abstract: background: the field of interventional cardiology have been marked by great advancements, but the way of dealing with information, passed on to the patients regarding the procedures, has been put aside. it is imperative to consolidate the orientation transmitted to the patients, instructing them about the benefits which can be attained through their understanding of invasive procedures. our objective was to assess the knowledge of patients submitted to cardiac catheterization in a specialized cardiology hospital. method: cross-section study, performed through interviews involving 11 questions related to the understanding of the patients regarding their disease, about the benefits and potential complications of the exam, and what care should be taken during their recovery. a score was developed and a cut-off point was established at 8 right answers to identify patients with a satisfactory level of knowledge. results: one hundred and forty eight patients were assessed, with a mean age of 60 ± 10 years, 55.4% male gender and of which 67.6% were being submitted to cardiac catheterism for the first time. the average number of right answers was 5.7 ± 2.0 and 17.6% of the patients achieved a score considered of satisfactory knowledge. patients with higher income and education levels showed a better performance in the score. conclusion: the results of this study allow us to conclude that the patients demonstrated unsatisfactory knowledge regarding the outpatient catheterization procedure. these results stress the importance of expanding the activities of health care teams, as a way of reducing this scenario of misinformation.
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