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Protective Effects of Some Medicinal Plants from Lamiaceae Family Against Beta-Amyloid Induced Toxicity in PC12 Cell
P Balali,M Soodi,S Saeidnia
Tehran University Medical Journal , 2012,
Abstract: Background: Excessive accumulation of beta-amyliod peptide (Aβ), the major component of senile plaques in Alzheimer's disease (AD), causes neuronal cell death through induction of oxidative stress. Therefore, antioxidants may be of use in the treatment of AD. The medicinal plants from the Lamiaceae family have been widely used in Iranian traditional medicine. These plants contain compounds with antioxidant activity and some species in this family have been reported to have neuroprotective properties. In the present study, methanolic extract of seven plants from salvia and satureja species were evaluated for their protective effects against beta-amyloid induced neurotoxicity.Methods: Aerial parts of the plants were extracted with ethyl acetate and methanol, respectively, by percolation at room temperature and subsequently, methanolic extracts of the plants were prepared. PC12 cells were incubated with different concentrations of the extracts in culture medium 1h prior to incubation with Aβ. Cell toxicity was assessed 24h after addition of Aβ by MTT assay.Results: Satureja bachtiarica, Salvia officinalis and Salvia macrosiphon methanolic extracts exhibited high protective effects against Aβ induced toxicity (P<0.001). Protective effects of Satureja bachtiarica and Salvia officinalis were dose-dependent.Conclusion: The main constituents of these extracts are polyphenolic and flavonoid compounds such as rosmarinic acid, naringenin, apigenin and luteolin which have antioxidant properties and may have a role in neuroprotection. Based on neuroprotective effect of these plants against Aβ induced toxicity, we recommend greater attention to their use in the treatment of Alzheimer disease.
Hepatoxicity of aqueous extract and fractionated methanol extract of Phytolacca americana by isolated rat liver perfusion system
M Karami, F Naghshvar, S Saeidnia, N Omrani
African Journal of Biotechnology , 2010,
Abstract: Recombinant retroviral vector containing human tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) gene was constructed and investigation of the in vitro invasion and metastasis of gastric cancer cells transfected with TIMP-2 was carried out. Human TlMP-2 was isolated from recombinant vector Bluescript 1/TIMP-2(+), and then inserted into the retroviral vector pL-MT. Correct orientation was verified by restriction endonuclease digestion. Human full length TIMP-2 gene was ligated into a plasmid, which was then transfected into PA317 cell line. G418-resistant individual clones were selected to transfect human SGC-7901 cell line. Cell proliferation, cell electrophoresis, soft agar colony formation and in vitro invasion were detected to analyze the bio-behavioral changes of cancer cells. The results from restriction endonuclease digestion were as theoretically expected. The cell electrophoresis rate, colony number and invasion ability in SGC-7901 cells and MFC cells transfected with TIMP-2 gene were significantly decreased when compared with control group. However, no significant changes were noted in the proliferation of cancer cells. We successfully construct a recombinant retroviral vector containing human TIMP-2. TIMP-2 transfection could markedly alter the membrane charge of cancer cells, resulting in decreased electrophoresis capacity, cell migration and invasion. However, cell growth was not affected by TIMP-2. These results suggested TIMP-2 transfection might exert effects on the malignant phenotype of cancer cells through affecting extracellular environment, which provided a new way to investigate gene regulation of in vitro collagen metabolism.
Immunosuppressive principles from Achillea talagonica, an endemic species of Iran
S Saeidnia,N Yassa,R Rezaeipoor,A Shafiee
DARU : Journal of Pharmaceutical Sciences , 2009,
Abstract: ABSTRACT Background and the purpose of study: Achillea talagonica Boiss. (Asteraceae) grows in the western and central parts of Iran. This plant has long been used in traditional medicine as an anti-inflammatory agent for treatment of rheumatic pain. Previously, the immunosuppressive activity of the aqueous extract of this endemic plant in experimental animals was reported. In this research, isolation of the main immunologically active components of A. talagonica, which were effective on humoral immune responses in BALB/c mice is elucidated. Methods: In order to find the main immunosuppressive components of A. talagonica, methanol and methanol-water (80% and 50% v:v) extracts were injected to BALB/c mice and the hemagglutinating antibody titer was assayed after immunization with SRBC (sheep red blood cells). Guided by this assay, active principles were separated by chromatographic methods. Results: Isolated compounds were identified as caffeic acid 9-O-glucoside (1), quercetin (2), luteolin (3), 3'-methoxy luteolin (4), proline (5) and choline (6) by comparison of their spectral data with those of reported in literatures. Immunosuppressive property of choline (5 mgkg-1) was comparable to those of prednisolone (10 mgkg-1); although, quercetin (20 mgkg-1) and caffeoyl glucoside (20 mgkg-1) decreased anti-SRBC titer in comparison with control groups. Major conclusion: Immunosuppressive effects of A. talagonica are due to some components belonging to betaine, flavonol and phenoilc esters.
A review on phytochemistry and medicinal properties of the genus Achillea
S Saeidnia,AR Gohari,N Mokhber-Dezfuli,F Kiuchi
DARU : Journal of Pharmaceutical Sciences , 2011,
Abstract: Achillea L. (Compositae or Asteraceae) is a widely distributed medicinal plant throughout the world and has been used since ancient time. Popular indications of the several species of this genus include treatment of wounds, bleedings, headache, infammation, pains, spasmodic diseases, fatulence and dyspepsia. Phytochemical investigations of Achillea species have revealed that many components from this genus are highly bioactive. There are many reports on the mentioned folk and traditional effects. Although, the medicinal properties of Achillea plants are recognized worldwide, there are only one review article mainly about the structures of the phytochemical constituents of Achillea. The present paper reviews the medicinal properties of various species of Achillea, which have been examined on the basis of the scientifc in vitro, in vivo or clinical evaluations. Various effects of these plants may be due to the presence of a broad range of secondary active metabolites such as favonoids, phenolic acids, coumarins, terpenoids (monoterpenes, sesquiterpenes, diterpenes, triterpenes) and sterols which have been frequently reported from Achillea species
Lignans and neolignans from Stelleropsis antoninae
AR Gohari,S Saeidnia,M Bayati-Moghadam,Gh Amin
DARU : Journal of Pharmaceutical Sciences , 2011,
Abstract: "n "n Background and the purpose of the study: Stelleropsis antoninae Pobed. (Family: Thymelaeaceae) grows wildly as an herbaceous plant in Iran. Most of the Thymelaeaceous plants contain lignans and neolignans, which have important pharmacologically properties. In the present study, the isolation and identification of the main lignans and neolignans of S. antoninae, which has not been previously reported is described and compared to other species. "nMethods: Column (CC) and High Performance Liquid Chromatographic (HPLC) methods were used for the isolation and purification, and 1H-NMR, 13C-NMR, HMBC, HMQC, H-H COSY and MS were employed for the identification of the compounds isolated from the methanol extract. "nResults: From the methanol extract of the aerial parts of S. antoninae four lignans, syringaresinol (1), syringaresinol 4-O-β-D-glucopyranoside (4), syringaresinol 4-O- β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside (5), liriodendrin (6), and two neolignans, 5-methoxylariciresinol 4'-O-β-D-glucopyranoside (3) and dehydrodiconiferyl alcohol 4-O-β -D-glucopyranoside (2) were isolated and identified. Major conclusion: The results of this study show that siringaresinol, a well-known bioavtive compound, and its glucosides are the main lignans, and lariciresinol and coniferyl alcohol derivatives are the main neolignans of S. antoninae.
Three phenolic glycosides and immunological properties of Achillea millefolium from Iran, population of Golestan
Yassa N.,Saeidnia S.,Pirouzi R.,Akbaripour M.
DARU : Journal of Pharmaceutical Sciences , 2007,
Abstract: From MeOH extract of aerial parts of Achillea millefolium L. collected from Golestan province of Iran, three glycosylated phenolic compounds, luteolin 7-O-glucoside, apigenin 7-O-glucoside and caffeic acid glucoside were isolated and identified by spectroscopic analyses. Immunological properties of different fractions of plant extract were studied on humoral immune system of BALB/c mice by Microhaemagglutination test. Among these fractions only two fractions at 125 mg kg-1 and 61.5 mg kg-1 showed a significant decrease in the anti- SRBC titer of mice (P<0.05). The immunological properties of the latter fractions may be due to glycosylated derivatives of caffeic acid.
In vitro antioxidant analysis of Achillea tenuifolia
H Asgarirad, F Pourmorad, SJ Hosseinimehr, S Saeidnia, MA Ebrahimzadeh, F Lotfi
African Journal of Biotechnology , 2010,
Abstract: Achillea tenuifolia (AT) is one of the most herbs are being used by people as a traditional medicinal remedy. Antioxidant activity of AT different extracts and total flavonoid and phenol levels in the extracts were investigated in this study. Plant extracts were prepared by maceration method using ethyl acetate, methanol and methanol-water (1:1). Folin Ciocalteu reagent in terms of gallic acid equivalent achieved the total phenol's content. AlCl3 was used as a reagent for flavonoid determination. Flavonoid content of the plant extracts obtained in terms of quercetin equivalent. DPPH radical scavenging effect of the extracts was determined by UV spectroscopy. Also in order to determine lipid peroxidation inhibition of the extracts of A. tenuifolia, ferric thiocyanate method with BHT, a synthetic reference standard, was carried out in this study. Phenol contents were 43.97 ± 0.034, 74.16 ± 0.55 and 106 ± 0.693 mg g-1 in the ethyl acetate, methanol and methanol-water extracts, respectively. Flavonoid amount obtained in the ethyl acetate, methanol and methanol-water extracts were 10.6 ± 1.85, 23.1 ± 0.5 and 190 ± 1.3 mg g-1, respectively. The percentage of DPPH radical scavenged by the most active extract (methanol-water) of A. tenuifolia was 92% at a concentration of 1 mgml-1 greater than 94% of BHT at 2 mgml-1. IC50 of methanol-water extract and BHT were 0.015 and 0.053 mgml-1, respectively. Lipid peroxidation inhibition was observed by the most polar extract of AT about 91.84%. Phenol and flavonoids content confirm the existence of more polar hydroxyl containing chemical structures in the plant. The potency of radical scavenging effect of methanol-water extract was about 3.5 times greater than synthetic antioxidant BHT. The inhibitory activity of the extracts on the lipid peroxidation of linoleic acid in ferric thiocyanate test was also significant (> 90%). In this study we concluded that there is a direct relation between phenol and flavonoid content of plant extracts and the antioxidant activity. So that the greater amount of phenolic compounds leads to more potent radical scavenging and lipid peroxidation inhibition activities as it was observed in A. tenuifolia polar extract in the present study.
Isolation of a new quinic acid derivative and its antibacterial modulating activity
A.R. Gohari,S Saeidnia,K Mollazadeh Moghaddam,N Yassa
DARU : Journal of Pharmaceutical Sciences , 2010,
Abstract: "nBackground and the purpose of the study: The species Hymenocrater calycinus, belongs to the plant family Lamiaceae and grows wildly in the north-east of Iran. Previously, the antimicrobial activity of the plant extracts was reported. In the present study, the bioactivity-guided fractionation of the methanol extract of H. calycinus and the combination effects of the isolated compound with cell wall active agents against S. aureus and E. coli was investigated. "n "nMethods: Column and thin layer chromatographic methods were used for isolation and purification and spectroscopic data (MS, 1H- and 13C-NMR, HMQC, HMBC and 1H-1H COSY) were employed for identification of the compound isolated from the extract. A disk diffusion method was used to determine the antibacterial activity of the isolated compound against S. aureus and E. coli in comparison with 7 different antibiotics.Results: The isolated compound 1 was identified as 3-(3, 4- dihydroxyphenyl) lactic acid 2-O-quinic acid. Compound 1 (500 μg/disc) enhanced antibacterial effect of ampicillin, ciprofloxacin, vancomycin and cefepime against S. aureus and activated the effects of ampicillin and vancomycin against E. coli. "nConclusion: Results showed that the compound 1 was not active against both tested strains at any concentration below 1 mg/disk, and as a result the enhancing effect of the compound could be due its association with antibiotics.
Who Plays Dual Role in Cancerous and Normal Cells? Natural Antioxidants or Free Radicals or the Cell Environment
Soodabeh Saeidnia,Mohammad Abdollahi
International Journal of Pharmacology , 2012,
Abstract: Not Available.
Application of spectroscopy in herbal metabolomics
Soodabeh Saeidnia, Ahmad Reza Gohari
DARU Journal of Pharmaceutical Sciences , 2012, DOI: 10.1186/2008-2231-20-91
Abstract:
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