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Search Results: 1 - 10 of 13972 matches for " Ruberval Armando Lopes "
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Histometric Study of Alveolar Bone in Rats Submitted to Ethanol During Lactation Estudio Histométrico del Hueso Alveolar en Ratas Sometidas a Etanol Durante la Lactancia
Viviane Curi,Reinaldo Azoubel,Ruberval Armando Lopes,Alex Tadeu Martins
International Journal of Morphology , 2008,
Abstract: The present work studied the adverse effects of maternal exposure of rats to alcohol during lactation, on the development of their off spring. Histometric evaluation by karyometry and of the alveolar bone at the level of the first upper molar of the sucking was perfomed. Two groups of animáis, one coming from mothers exposed to drinking water containing 20% ethanol during the total lactation period and the other of controls coming from mothers receiving only alcohol-free drinking water during this period. On the 21 first day of lactation the young of each group were aleatorily selected and following anesthesia, their heads severed; after histological treatment, serial 6 μm sections on the frontal pla e at the molar level, stained with hematoxilin and eosin, were obtained. The experimental results produced, suggest that sucking from ethanol-treated mothers, show retarded post-natal growth, their alveolar bones presenting scarce, little calcified trabeculae, and a more abundant bone marrow compared to controls. Considerando que la lactancia es un período importante y vulnerable, y que la exposición materna al alcohol durante esta fase puede provocar efectos adversos en las crías en desarrollo, el propósito del presente trabajo es evaluar histométricamente (mediante cariometría y estereología), el hueso alveolar a nivel del primer molar superior, de ratas lactantes sometidas a los efectos del etanol. Con esa finalidad utilizamos dos grupos, uno que recibió etanol al 20% en el bebedero, durante todo el período de lactancia (21días), y otro que recibió solo agua. En el 21° día de lactancia, 5 ratas de cada grupo fueron aleatoriamente seleccionadas. Después de sacrificados, los animales fueron fijados en formol al 10%. Las cabezas, separadas de los cuerpos, fueron incluidas en parafina, y cortadas seriadamente con 6 Lim de grosor, a partir de un plano frontal al nivel de los molares, y te idas con hematoxilina y eosina. Basados en los resultados presentes, es posible concluir que el etanol al 20% en el agua de beber, administrado a ratas lactantes, provoca los siguientes efectos: reducción significativa del peso corporal, alteraciones de las trabéculas del hueso alveolar, observadas estereológicamente, evidenciadas por trabéculas delicadas y poco calcificadas, con médula abundante, núcleos menores en osteoblastos y osteocitos y más achatados en los primeros, y con osteocitos de volúmenes citoplasmático y celular menor y densidad numérica mayor.
EFECTOS DEL CADMIO EN LA MUCOSA YUGAL DE LA RATA DURANTE LA LACTANCIA. ESTUDIO MORFOLóGICO E HISTOMéTRICO
Lara,Cristina Picoli; Watanabe,Ii-Sei; Lopes,Ruberval Armando; Sala,Miguel Angel; Picoli,Fábio;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000300002
Abstract: cadmiun in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. cadmium affects placental functions, can cross the trophoblastic barrier and directly disturb the fetal development. it is also excreted into milk. the organism is particularly susceptible to cadmium exposure at perinatal period. the effect on rat oral epithelium (upper vestible and floor of the mouth) after a continuous exposure to drinking water containing low level of cadmium during lactation was studied. female rats received drinking water containing 300 mg/l cdcl2 ad libitum during the whole lactation. control animals received a similar volume of water without cadmium. lactant rats (21 day-old) were killed by letal dosis of anesthetic. the head were separated, fixed in alfac solution for 24 h. palate and jaw regions were serially sectioned in frontal plane, at level of first molars, and the 6 μm thick sections were stained with hematoxylin and eosin. nuclear epithelium parameters were estimated, as web as cytoplasm and cell volume, nucleus/cytoplasm ration, number and surface density, and epithelial thickness. mean body weignt was 34.86 g for the control group and 18.56 g for the treated group. histologically, the epithelium was thinner, with more numerous and smaller cells. in this experiment, cadmium induced epithelial atrophy, indicating a direct action in oral mucosae cells, besides restricted development in pups
EFECTOS DE LA ADMINISTRACIóN PRENATAL DE CAFEíNA SOBRE LA GLáNDULA SUBMANDIBULAR DE FETOS DE RATA
de Lacerda,Suzie Aparecida; Sala,Miguel Angel; Lopes,Ruberval Armando; Semprini,Marisa; Watanabe,Ii-Sei;
International Journal of Morphology , 2003, DOI: 10.4067/S0717-95022003000100006
Abstract: the effects of a single intraperitoneal injection of caffeine (10 mg/kg body weight) in the 10th day of rat pregnancy on the fetus, especially on the submandibular gland, were studied. at the microscopic examination, it was observed immature submandibular glands, constituted by solid cords and terminal buds, in the fetuses from the treated group. morphometrically it was verified increased nuclear sizes in both the acinar and ductular cells of the injected group fetuses. the relative volumes occupied by either ducts and connective tissue were significantly higher in the submandibular glands of the injected group. no differences were demonstrated between both groups in the mean diameter of both acini and ducts, whereas the thickness of the acinar epithelium was lower in the treated group. the increased relative volume of ducts in the treated group fetuses, in spite of their similar diameters in both groups, is due, certainly, to an increase number of ducts in the submandibular gland in fetuses from rats injected with caffeine in the 10th day of pregnancy.
Effects of cigarette smoke on the Meckel's cartilage of rat fetus: morphologic, morphometric and stereologic study
Brandini, Daniela Atili;Sala, Miguel Angel;Lopes, Ruberval Armando;Semprini, Marisa;Contrera, Mary Garcia Duarte;
Brazilian Dental Journal , 2005, DOI: 10.1590/S0103-64402005000100011
Abstract: the purpose of this study was to investigate the effects of cigarette smoke on the development of the embryo mandible (meckel's) cartilage in rat fetuses. when inhaled by female wistar rats between the 9th and the 12th day of pregnancy, cigarette smoke (5 cigarettes a day) caused intrauterine growth retardation, providing smaller fetuses and placentas. in fetuses from the experimental group, the histopathologic examination revealed a poorly developed meckel's cartilage with smaller chondroblasts showing a scanty cytoplasm with spherical and paler central nuclei, as well as more abundant cartilage matrix. morphometric analysis revealed that meckel's cartilage lacunae were smaller in the fetuses from the experimental group, although not showing any remarkable alteration in shape. the results suggested that inhalation of cigarette smoke by pregnant rats during the organogenic period induced growth retardation and delayed cellular differentiation in rat fetal meckel's cartilage.
Effect of cadmium on the floor of the mouth on rats during lactation
Picoli, Lara Cristina;Watanabe, Ii-Sei;Lopes, Ruberval Armando;Sala, Miguel Angel;Picoli, Fábio;
Brazilian Oral Research , 2004, DOI: 10.1590/S1806-83242004000200003
Abstract: cadmium (cd) present in the air, drinking water and food has the potential to affect the health of people, mainly those who live in highly industrialized regions. cd affects placental function, may cross the placental barrier and directly modify fetal development. it is also excreted into milk. the body is particularly susceptible to cd exposure during perinatal period. the effect on rat oral epithelium (floor of the mouth) after continuous exposure to drinking water containing low levels of cd during lactation was studied. female rats were supplied with ad libitum drinking water containing 300 mg/l of cdcl2 throughout the whole lactation period. control animals received a similar volume of water without cd. lactating rats (21 day-old) were killed by lethal dose of anesthetic. the heads were retrieved, fixed in "alfac" solution (alcohol, acetic acid and formaldehyde) for 24 h, serially sectioned in frontal plane, at the level of the first molars. the 6 μm sections were then stained with hematoxylin and eosin. nuclear epithelium parameters were estimated, as well as cytoplasm and cell volume, nucleus/cytoplasm ratio, numeric and surface densities, and epithelial thickness. mean body weight was 34.86 g for the control group and 18.56 g for the cd-treated group. histologically, the floor of the mouth epithelium was thinner in the treated group, with smaller and more numerous cells. in this experiment, cd induced epithelial hypotrophy, indicating a direct action in oral mucosa cells, besides retarded development of the pups.
Efecto de la infección aguda por diferentes cepas de Trypanosoma cruzi sobre el crecimiento intrauterino de fetos de ratón
SALA,MIGUEL ANGEL; LOPES,RUBERVAL ARMANDO; CARRARO ABRAH?O,ANA AMéLIA; MENEGUETTE,CíCERO;
Parasitología latinoamericana , 2006, DOI: 10.4067/S0717-77122006000100010
Abstract: the objective of this work was to evaluate the effects of the acute maternal infection with different strains of trypanosoma cruzi on the intrauterine growth of the mouse fetus. pregnant mice, 60 day-old, were i.p. inoculated with 2 x 105 trypomastigotes blood stream of t. cruzi (colombian, y, bolivia or rc strains). the results allow to conclude that the t. cruzi infection affect the intrauterine growth of the mouse fetus, with reduction of both weight and length, umbilical cord length, body weight to placenta weight ratio and body weight to umbilical cord length ratio. t. cruzi strains showed different behavior. in general, the less prejudicial to the fetal growth was the rc strain, whereas the most prejudicial were the bolivia and y strains. the parasitemic curves of these strains show the most rapid growth, reaching the peak at the 5th and 7th day, respectively. as inoculation was made to agree the last pregnancy day with the parasitemic peak, the infected animals had the most rapid infection with elevated parasitemy, without possibility of maternal adaptation
Histological and morphometric investigations of changes induced by the RAL strain of Trypanosoma cruzi in the mouse placenta
Abrah?o, Ana Amélia Carraro;Meneguette, Cícero;Sala, Miguel Angel;Lopes, Ruberval Armando;
Revista Brasileira de Ciências Farmacêuticas , 2003, DOI: 10.1590/S1516-93322003000200007
Abstract: pregnant swiss mice (mus musculus) were inoculated intraperitoneally with 2 x 105 trypomastigotes of the ral strain of trypanosoma cruzi on the 7th day of pregnancy and sacrificed on the 19th day of pregnancy. the placenta was sectioned for the assessment of histological and morphometric changes. the ral strain showed intense tropism for the placenta, with parasitism reaching the three placental layers. there was involvement of the maternal and fetal portions of the placentas, and also of giant cells and spongioblasts. the placentas of infected animals presented sparse areas of degeneration and necrosis, with mild dystrophic calcification of the decidua. the inflammatory process consisted of plasmocytes and lymphocytes, revealing involvement of the decidua. cytometric study of giant trophoblastic cells showed that the placentas of the infected group were seriously affected, also with respect to cell volume. the changes provoked by the ral strain in the trophoblastic cells and the difference in behavior observed in the cell population of the various placental regions may affect intrauterine development, probably by a deficient production of hormones such as placental lactogen, which acts as a fetal growth hormone, or indirectly by deficient tissue invasion caused by inefficient utero-placental vascularization, thus impairing fetal nutrition.
Efecto de la infección aguda por diferentes cepas de Trypanosoma cruzi sobre el crecimiento intrauterino de fetos de ratón EFFECT OF THE ACUTE INFECTION BY DIFFERENT STRAINS OF Trypanosoma cruzi INTRAUTERINE GROWTH OF THE MOUSE FETUSES
MIGUEL ANGEL SALA,RUBERVAL ARMANDO LOPES,ANA AMéLIA CARRARO ABRAH?O,CíCERO MENEGUETTE
Parasitología latinoamericana , 2006,
Abstract: El objetivo de este trabajo fue evaluar los efectos de la infección aguda materna con diferentes cepas de T. cruzi sobre el crecimiento intrauterino del ratón. Animales grávidos, de 60 días, fueron inoculados i.p. con 2 x 10(5) tripomastigotes sanguíneos de T. cruzi (cepa colombiana, Y, Bolivia o RC). Los resultados muestran que la infección afecta el crecimiento intrauterino, con reducción de peso y de longitud del cuerpo, longitud del cordón umbilical, relación peso corporal/peso placentario y relación peso corporal/longitud del cordón umbilical. Las cepas usadas muestran comportamiento diferente. En general, la menos perjudicial para el crecimiento fue la cepa RC, mientras que las más deletéreas fueron las cepas Bolivia e Y. Las curvas parasitemicas de estas cepas tienen el crecimiento más rápido, alcanzando el máximo al 5o y 7o día, respectivamente. Como la inoculación fue hecha de modo a coincidir los niveles máximos de parasitemia con el fin de la gravidez, estas cepas causan un proceso infeccioso más rápido, con altos índices de parasitemia, sin permitir la adaptación materna The objective of this work was to evaluate the effects of the acute maternal infection with different strains of Trypanosoma cruzi on the intrauterine growth of the mouse fetus. Pregnant mice, 60 day-old, were i.p. inoculated with 2 x 10(5) trypomastigotes blood stream of T. cruzi (Colombian, Y, Bolivia or RC strains). The results allow to conclude that the T. cruzi infection affect the intrauterine growth of the mouse fetus, with reduction of both weight and length, umbilical cord length, body weight to placenta weight ratio and body weight to umbilical cord length ratio. T. cruzi strains showed different behavior. In general, the less prejudicial to the fetal growth was the RC strain, whereas the most prejudicial were the Bolivia and Y strains. The parasitemic curves of these strains show the most rapid growth, reaching the peak at the 5th and 7th day, respectively. As inoculation was made to agree the last pregnancy day with the parasitemic peak, the infected animals had the most rapid infection with elevated parasitemy, without possibility of maternal adaptation
PALATINE MUCOSA ANGIOARCHITECTURE STUDY OF YOUNG RABBITS USING SCANNING ELECTRON MICROSCOPY ESTUDIO DE LA ANGIOARQUITECTURA DE LA MUCOSA PALATINA DE CONEJOS JóVENES USANDO MICROSCOPíA ELECTRóNICA DE BARRIDO
Marcia Consentino Kronka,Ii-Sei Watana,Marcelo Cavenaghi Pereira da Silva,Ruberval Armando Lopes
Revista chilena de anatomía , 2000,
Abstract: A few papers have been related to young animal's angioarchitecture. This study tries to contribute to research about development of three-dimensional distribution in masticatory mucosa. The vascular corrosion casts were obtained using low viscosity resin - Mercox CL-2B. We could note specific networks at dome of palatine rugae, inter-rugae areas and labial mucosa. Capillary loops were observed only at transverse palatine rugae Pocos trabajos han relatado la angioarquitectura de animales jóvenes. Este estudio contribuye con las investigaciones acerca de la distribución tridimensional de la mucosa palatina en conejos jóvenes usando MEB. La corrosión vascular del molde fue obtenida usando resina de baja densidad - Mercox CL-2B. No pudimos observar redes específicas en la cúpula de los pliegues palatinos, en los interpliegues y mucosa labial. Fueron observados solamente loops capilares y pliegues transversos palatinos
Morfometría de la Parótida de Ratón Infectado por la Cepa RAL de Trypanosoma cruzi en Función del Sexo Morphometry of the Parotid Gland in Mice Infected by the RAL Strain of Trypanosoma cruzi in Function of the Sex
Sergio de Albuquerque,Ruberval Armando Lopes,Miguel Angel Sala,Ana Amelia Carraro Abraháo
International Journal of Morphology , 2007,
Abstract: Fueron estudiadas las alteraciones y el nivel de deterioro de las parótidas de animales infectados con la cepa RAL de Trypanosoma cruzi. Se utilizaron ratones albinos (Mus musculus) de ambos sexos, debido al dimorfismo sexual de las glándulas salivares, inoculados con 2 x 10(4) tripomastigotes sanguíneos de la cepa RAL del T. cruzi. Los animales fueron sacrificados al 12° día de infección, coincidiendo con el pico parasitémico. La parótida fue procesada histológicamente y, posteriormente, evaluada histopatológica y morfometricamente. Los resultados permitieron verificar intenso parasitismo en la glándula, la que presentó desorganización estructural y atrofia generalizada de acinos y conductos, más intensos en las hembras. Concluyendo, la cepa RAL del T. cruzi mostró un comportamiento atípico en relación a otras cepas, causando modificaciones más evidentes en las hembras, debido, posiblemente, a alteraciones hormonales desencadenadas por el T. cruzi They were studied the alterations and the level of deterioration of the parotid gland in mice infected with the RAL strain oí Trypanosoma cruzi. They were used albino mice (Mus musculus) of both sexes, due to the existence of sexual dimorphism of the salivary glands, inoculated with 2 x 10(4) blood trypomastigotes of the RAL strain of T cruzi. The animals were sacrificed at the 12th day of infection, coinciding with the parasitemic peak, and the parotid gland was histologically processed and histopathologically and histometrically studied. The results allow verifying intense parasitism in the parotid gland with structural disorganization and widespread atrophy of acini and ducts, more marked in the females. Concluding, the RAL strain of T cruzi shows an atypical behavior in relation to other strains, provoking more clear modifications in the females, probably due to the hormonal alterations motivated by the T cruzi
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