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Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (Lupinus albus L.) Represent a Unique Genepool  [PDF]
Rosy Raman, Raymond B. Cowley, Harsh Raman, David J. Luckett
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.42012

PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.

Genetic Diversity and Lineage Based on SSR Markers of Two Genomic Resources among Trifolium Collections Held within the Australian Pastures Genebank  [PDF]
Devendra R. Malaviya, Harsh Raman, Brian S. Dear, Rosy Raman, Ajoy K. Roy, Pankaj Kaushal, Amaresh Chandra, Steve J. Hughes
Open Journal of Genetics (OJGen) , 2019, DOI: 10.4236/ojgen.2019.91001
Abstract: Trifolium alexandrinum, an important forage legume, suffers from narrow genetic base. The present investigation was envisaged to reveal the inter- and intra-species genetic diversity and lineage among 64 accessions, representing a global collection, of T. alexandrinum; it’s two probable progenitor species (T. salmoneum and T. subterraneum) and the three genetically distant species (T. repens, T. vesiculosum, T. michelianum). A set of Simple Sequence Repeats (SSR) primer-pairs developed from T. alexandrinum have shown to amplify alleles across the species under study, suggesting utility of the newly developed resource for assessing molecular diversity among Trifolium species. These SSRs markers together with previously reported SSRs, derived from T. repens, enabled to reveal high intra-species polymorphism in T. alexandrinum and successfully discriminate different species investigated in this study. The diverse accessions determined herein provide a superior resource for further breeding
Localisation of Loci Involved in Resistance to Diaporthe toxica and Pleiochaeta setosa in White Lupin (Lupinus albus L.)  [PDF]
Rosy Raman, Cina Vipin, David J. Luckett, Raymond B. Cowley, Gavin J. Ash, John D. I. Harper, Andrzej Kilian, Harsh Raman
Open Journal of Genetics (OJGen) , 2014, DOI: 10.4236/ojgen.2014.43022

L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. Phomopsis blight (PB) and Pleiochaeta root rot (PRR), caused by the fungal pathogens Diaporthe toxica and, Pleiochaeta setosa respectively, are two major yield-limiting diseases of the L. albus crop. The extent of genetic diversity in 94 accessions of L. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing PCR-based genic, and microarray-based Diversity Arrays Technology (DArT), markers. All accessions were evaluated for resistance to PB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to PRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic- and 674 DArT) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in PB and PRR resistance using linear regression analysis. Several markers showed significant association with PB or PRR resistance at P < 0.05. Our results showed that favourable alleles for PB and PRR resistance are present in the diverse accessions investigated and they will provide valuable materials for lupin breeding.

Rosy Jan
Trends in Information Management , 2006,
Abstract: The study compares nine constituent gateways of Intute: Health and Life Sciences by submitting select Biotechnology related terms. The results reveal that "Bioethicsweb" contain largest number of resources on'cloning' and "Vetgate— retrieves largest number of resources for 'Antibiotics' while great strength of resources on select term 'Gene' is found in "Bioresearch'' gateway. However, the intute' provides a platform to retrieve results from all the nine gateways in a single interface. It is observed that the 'Intute' supports Boolean, Truncation and Phrase searching. The results further reveal that significant resources are not retrieved while searching on resource type basis. KEYWORDS : Biotechnology; Subject gateways; Quality resources
Preservation of Library, Archival and Digital Documents: Problems and Perspectives Ramaiah, L.S., & Sujatha, G. (Eds.). New Delhi: Ess Ess Publications, 2008. XXXIV+499. Rs. 1300. ISBN 81-7000-538-8 (Hard Bound)
Rosy Jan
Trends in Information Management , 2009,
May, Christopher. (2008). Digital Rights Management: The Problem of Expanding Ownership Rights. Oxford: Chandos, xv+ 162p. $ 69.95. ISBN 1-84334-124-7 (Paper Back).
Rosy Jan
Trends in Information Management , 2008,
Citation Analysis of Library Trends
Rosy Jan
Webology , 2009,
Abstract: Citation analysis of all the journal articles published in the Library Trends from 1994-2007 is carried out. 593 articles are published in the journal during 14 years. Highest number (52) of articles is published in 2004. The Journal contained 15662 references for the study period of which 13783 are p-citations and 1879 are e-citations. Every issue published approx. 11 articles and each article has an average of 23.2 p-references and 3.1 e-citations. It was found that 44.51 % print books are consulted by the authors and 0% e-books are accessed. Authors have consulted 44.04% p-journals as against 11.82% e-journals. Figures shows that 88.14% other web references are used in the articles reference. Female contribution (52.34%) accounts more than male contribution (47.66%).
Exact Solutions of the SU(2) Yang-Mills-Higgs Theory
Rosy Teh
Physics , 2000, DOI: 10.1142/S0217751X01004906
Abstract: Some exact static solutions of the SU(2) Yang-Mills-Higgs theory are presented. These solutions satisfy the first order Bogomol'nyi equations, and possess infinite energies. They are axially symmetric and could possibly represent monopoles and an antimonopole sitting on the z-axis.
Genome-Wide Delineation of Natural Variation for Pod Shatter Resistance in Brassica napus
Harsh Raman, Rosy Raman, Andrzej Kilian, Frank Detering, Jason Carling, Neil Coombes, Simon Diffey, Gururaj Kadkol, David Edwards, Margaret McCully, Pradeep Ruperao, Isobel A. P. Parkin, Jacqueline Batley, David J. Luckett, Neil Wratten
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0101673
Abstract: Resistance to pod shattering (shatter resistance) is a target trait for global rapeseed (canola, Brassica napus L.), improvement programs to minimise grain loss in the mature standing crop, and during windrowing and mechanical harvest. We describe the genetic basis of natural variation for shatter resistance in B. napus and show that several quantitative trait loci (QTL) control this trait. To identify loci underlying shatter resistance, we used a novel genotyping-by-sequencing approach DArT-Seq. QTL analysis detected a total of 12 significant QTL on chromosomes A03, A07, A09, C03, C04, C06, and C08; which jointly account for approximately 57% of the genotypic variation in shatter resistance. Through Genome-Wide Association Studies, we show that a large number of loci, including those that are involved in shattering in Arabidopsis, account for variation in shatter resistance in diverse B. napus germplasm. Our results indicate that genetic diversity for shatter resistance genes in B. napus is limited; many of the genes that might control this trait were not included during the natural creation of this species, or were not retained during the domestication and selection process. We speculate that valuable diversity for this trait was lost during the natural creation of B. napus. To improve shatter resistance, breeders will need to target the introduction of useful alleles especially from genotypes of other related species of Brassica, such as those that we have identified.
Space Weather – Sun Earth Relations  [PDF]
K. Sundara Raman
International Journal of Astronomy and Astrophysics (IJAA) , 2011, DOI: 10.4236/ijaa.2011.11003
Abstract: Sun, a star of spectral type G2 is the main source of energy to the Earth. Being close to the Earth, Sun pro-duces a resolvable disk of great detail, which is not possible for other stars. Solar flares and coronal mass ejections are the enigmatic phenomena that occur in the solar atmosphere and regularly bombard the Earth’s environment in addition to the solar wind. Thus it becomes important for us not only to understand these physical processes of the Sun, but in addition how these activities affect the Earth and it’s surrounding. Thus a branch of study called ‘Space Weather’ had emerged in the recent past, which connects the Sun Earth rela-tions. This paper details about the solar activity and associated energetic phenomena that occur in the atmosphere of the Sun and their influence on the Earth.
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