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Search Results: 1 - 10 of 412285 matches for " Rosa M.; Salgado Arocena "
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Aislamiento de células mononucleares de sangre periférica para trasplante de células madre: Método simplificado
Cortina Rosales,Lázaro; Hernández Ramírez,Porfirio; López De Roux,Maríadel Rosario; Artaza Sanz,Heriberto M; Dorticós Balea,Elvira; Macías Abraham,Consuelo; Socarrás Ferrer,Berta B; Lam Díaz,Rosa María; González Suárez,Tania; Matamoros,María de los ángeles; González Iglesias,Ana Iris; Salgado Arocena,Odalis; Hernández Rego,Yakima;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2008,
Abstract: in the last years, the topic of stem cells has arisen an increasing interest for its therapeutic potential in diseases that have not an effective treatment so far. a prospsective and exploratory study was conducted in patients with obliterant atherosclerosis of the lower limbs to evaluate the safety and effectiveness of a manual method of collection and processing of mononuclear cells and of cd34+ cells, starting from mobilized peripheral blood. the blood was processed in closed collection systems, using hydroxyethyl starch as a potentiator of erythrocyte sedimentation. the patients were treated with granulocyte colony stimulating factor at total doses of 40μcg/kg of weight during 2 days. self-donation was performed when the leukocyte count was higher than 20 x 109/l. to assess the efficacy of the method, the amounts of nucleated cells, of mononuclear cells and of cd 34+ cells in the cellular concentrate were analyzed. cellular viability was determined and a microbiological study of the material obtained was conducted. it was proved that the method is efficient and safe, since adequate cellular levels with a high viability and absence of bacterial contamination are attained. on the other hand, it is simple and cheap, which allows its application in other health centres, particularly in those with less resources. this makes possible that more patients benefit from the stem cell treatment.
Implante percutáneo de células mononucleares de sangre periférica movilizadas con factor estimulante de colonias granulocíticas, en la osteoartrosis de rodilla. Primer caso comunicado en Cuba
Baganet Cobas,Aymara María; Hernández Ramírez,Porfirio; Fernández Delgado,Norma; Forrellat Barrios,Mariela; González Iglesias,Ana Iris; Pérez Fuentes,Enrique; Macías Abraham,Consuelo; Dorticós Balea,Elvira; Lam Díaz,Rosa M.; Salgado Arocena,Odalys; Villanueva Cagigas,Emilio; León Valladares,Dayneri; Smith García-Menocal,Héctor; Pozo Alonso,Desi; álvarez Hernández,Roberto; Simón Pita,Ana María;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2010,
Abstract: the degenerative joint disease, also known as osteoarthrosis affects to 10% of elderlies aged 60. it is mainly characterized by pain in the involved joint, crepitation, morning stiff and a progressive limitation of movement of that joint leading to a partial or total wear of articular cartilage. the treatment of the knee osteoarthrosis is a great challenge. the recent advances in use of regenerative medicine suggest that adult stem cells could represent a promisor alternative in the treatment of this entity. in a female patient aged 61 presenting with knee osteoarthrosis authors placed a percutaneous implant of autologous mononuclear cells mobilized to peripheral blood by granulocyte colony-stimulating factor achieving a fast clinical and radiological improvement. this result suggests that the procedure used is a feasible, simple, safe and less expensive method for treatment of articular degenerative lesions.
Una experiencia con maestros rurales: Triangulando la evaluación: una reflexión sobre las reflexiones de los docentes en el proceso formativo
Arocena Gómez,Marcela; Guantay,Rosa Evelia;
Cuadernos de la Facultad de Humanidades y Ciencias Sociales. Universidad Nacional de Jujuy , 2000,
Abstract: in this work we recovers the word of teachers, directors and supervisors of the departments of iruya and santa victoria (county of salta - argentina), raised in an evaluation device foreseen inside the mark of an experience of continuous formation among the teachers of rural schools of the mentioned area and the participants of the workshop iv of the faculty in sciences of the education of the unsa., formalized in a project of investigation and participant development that it extends from the year 1993 at 1996. put on practice the triangulation of the evaluation of the teachers arises like demand of the own project with the objective of contributing to the reflection about the reflections of the teachers in the formative process. the methodological option was "to read the word of the teachers like a transactional act" (dubois, 1985) in the one that the reflections - evaluations of the teachers - it establish a dialectical relationship with our own reflection - evaluation of the successive approaches to the definition of the educational practice in the rural schools. to recover the practice of the teachers: "what" they say, "how" they say it, without neglecting "when" and "from where"; this allowed to refer the practices to coordinates of time (historical process) and space (performance contexts), finding movements and permanent restructurings in the identity of the participants that these converge in an approach of the me to the us. we identify four key moments that give bill of becoming dialectical of the history of the group and of the group processes generated in their breast. a) of personal crisis - professional b) of reconceptualization of itself and of the practice c) of consolidation of the me in the group d) of crisis starting from the conflicts generated by the personal and professional growth at the end of the training process, the rural teachers begin to register in their experience the construction of new "miradors" from those which to contemplate what h
Rendimento de piment?o em fun??o da aduba??o organica e mineral
Sediyama, Maria Aparecida N;Vidigal, Sanzio M;Santos, Marlei Rosa dos;Salgado, Luís Tarcísio;
Horticultura Brasileira , 2009, DOI: 10.1590/S0102-05362009000300006
Abstract: this experiment was carried out in a field in oratorios, minas gerais state, brazil, to evaluate the effect of organic fertilization associated to mineral fertilization on the production of pepper. the experiment was arranged in the randomized block design, with four replications, in a 4 x 3 factorial scheme; four doses of organic fertilizer (0; 30; 60 and 90 t ha-1) three doses of mineral fertilization (0; 750 and 1500 kg ha-1 of the npk 4-14-8 formulation applied to furrows at planting). twenty-eight pepper seedlings, cultivar magda, were distributed into each 14 m2 plot, at 1.0x0.5 m spacing. nutrient contents were evaluated in the leaves at the beginning of flowering, production of total, commercial and non-commercial fruits were determined. commercial fruits were classified based on the length and diameter, into extra, special and first class. the extra class accounted for most of the commercial fruit production. fertilization with organic compost was efficient for pepper nutrition, increasing pepper yield. the mineral fertilizer had additional effect for fruit production. the highest yield of commercial fruits was estimated when associated 84.43 t ha-1 of composed organic with the highest dose of mineral fertilizer.
Organic Amendments and Earthworm Addition Improve Properties of Nonacidic Mine Tailings
P. M. Rutherford,J. M. Arocena
Applied and Environmental Soil Science , 2012, DOI: 10.1155/2012/395964
Abstract: In many mined areas, lack of topsoil limits conversion of disturbed landscapes to former or other productive uses. We examined the use of biosolids (10 or 20% by dry mass), with or without sawdust, pulp sludge, and the contribution of an earthworm species (Dendrobaena veneta) to improve the properties of nonacidic mine tailings. Pulp sludge more rapidly immobilized excessive concentrations from biosolids early in the study; however, total mineral N concentrations were similar in pulp sludge and sawdust treatments by week 29. Although -N concentrations were generally greater in treatments with earthworms, these trends were not statistically significant ( ). In general, Bray P concentrations were greater in the presence of earthworms. Soil thin sections showed that earthworms mixed organic residues into elongated spherical units within mine tailings. Organic residues in combination with earthworm addition may improve the chemical and microstructural properties of non-acidic mine tailings, producing a substrate conducive for plant establishment. 1. Introduction Mining operations around the world produce large quantities of residuals such as tailings, overburden, and waste rock. Past mineral extractions in the Sierra de Cartagena (SE Spain) for >2,500 years left behind large volumes (i.e., usually 700,000–900,000?m3 per deposit) of wastes composed of overburden rocks and tailings [1]. A copper and gold mine in northern British Columbia (Canada) generated nearly 25 million tonnes of milled waste rock in 2007 [2]. In Canada and other jurisdictions, industry is required to convert disturbed landscapes to former or other productive uses following mine extraction and processing activities. High-quality topsoil is often limiting at mine sites, so mines often need to utilize on-site substrates (such as mine tailings) to create mine soils for reclamation activities. Establishment of vegetation is key to reclamation of mine tailings and other residuals [2–5]. Plant cover minimizes the dispersion of particulate matter and contaminants through wind and water erosion, and improves the aesthetic value of unvegetated landscapes [6, 7]. However, mine soils require suitable physical and chemical properties in order to support the growth of plants and associated soil organisms. Tailings are typically fine sands low in organic matter and plant available macronutrients such as N and P. Various industrial, environmental, and municipal waste treatment facilities generate organic materials that can be added to mine residuals to increase soil organic matter (SOM) and/or nutrient
Expression of VjbR under Nutrient Limitation Conditions Is Regulated at the Post-Transcriptional Level by Specific Acidic pH Values and Urocanic Acid
Gastón M. Arocena, Angeles Zorreguieta, Rodrigo Sieira
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035394
Abstract: VjbR is a LuxR homolog that regulates transcription of many genes including important virulence determinants of the facultative intracellular pathogen Brucella abortus. This transcription factor belongs to a family of regulators that participate in a cell-cell communication process called quorum sensing, which enables bacteria to respond to changes in cell population density by monitoring concentration of self produced autoinducer molecules. Unlike almost all other LuxR-type proteins, VjbR binds to DNA and activates transcription in the absence of any autoinducer signal. To investigate the mechanisms by which Brucella induces VjbR-mediated transcriptional activation, and to determine how inappropriate spatio-temporal expression of the VjbR target genes is prevented, we focused on the study of expression of vjbR itself. By assaying different parameters related to the intracellular lifestyle of Brucella, we identified a restricted set of conditions that triggers VjbR protein expression. Such conditions required the convergence of two signals of different nature: a specific pH value of 5.5 and the presence of urocanic acid, a metabolite involved in the connection between virulence and metabolism of Brucella. In addition, we also observed an urocanic acid, pH-dependent expression of RibH2 and VirB7, two additional intracellular survival-related proteins of Brucella. Analysis of promoter activities and determination of mRNA levels demonstrated that the urocanic acid-dependent mechanisms that induced expression of VjbR, RibH2, and VirB7 act at the post-transcriptional level. Taken together, our findings support a model whereby Brucella induces VjbR-mediated transcription by modulating expression of VjbR in response to specific signals related to the changing environment encountered within the host.
MRI and endoscopic ultrasonography in the staging of gastric cancer Resonancia magnética y ecoendoscopia para el estadiaje del cáncer gástrico
M. G. Arocena,A. Barturen,L. Bujanda,O. Casado
Revista Espa?ola de Enfermedades Digestivas , 2006,
Abstract: Objective: to determine the diagnostic precision of endoscopic ultrasounds (EUS) and magnetic resonance imaging (MRI) in the preoperative staging of gastric cancer. Methods: a prospective, blind study was carried out in 17 patients diagnosed with gastric cancer (GC) using endoscopic biopsy from November 2002 to June 2003. Patients underwent preoperative MRI and EUS. The reference test used was pathology, and laparotomy for non-resectable cases. Results: MRI (53%) was better than EUS in the assessment of gastric wall infiltration (35%). MRI (50%) was also superior to EUS (42%) for N staging. After pooling stages T1-T2 and T3-T4 together, results improved for both MRI (67 and 87.5%, respectively) and EUS (67 and 62.5%, respectively) (p < 0.05). N staging -lymph node invasion- results were correct in 50% for MRI as compared to EUS (42%). In classifying positive and negative lymph nodes EUS was superior to MRI (73 versus 54%). Conclusions: MRI was the best method in the assessment of gastric wall infiltration. EUS was superior to MRI for T1 staging, and in the assessment of lymph node infiltration.
Fragment-Hopping-Based Discovery of a Novel Chemical Series of Proto-Oncogene PIM-1 Kinase Inhibitors
Gustavo Saluste, Maria I. Albarran, Rosa M. Alvarez, Obdulia Rabal, Miguel Angel Ortega, Carmen Blanco, Guido Kurz, Antonio Salgado, Paolo Pevarello, James R. Bischoff, Joaquin Pastor, Julen Oyarzabal
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045964
Abstract: A new chemical series, triazolo[4,5-b]pyridines, has been identified as an inhibitor of PIM-1 by a chemotype hopping strategy based on a chemically feasible fragment database. In this case, structure-based virtual screening and in silico chemogenomics provide added value to the previously reported strategy of prioritizing among proposed novel scaffolds. Pairwise comparison between compound 3, recently discontinued from Phase I clinical trials, and molecule 8, bearing the selected novel scaffold, shows that the primary activities are similar (IC50 in the 20 to 150 nM range). At the same time, some ADME properties (for example, an increase of more than 45% in metabolic stability in human liver microsomes) and the off-target selectivity (for example, an increase of more than 2 log units in IC50 vs. FLT3) are improved, and the intellectual property (IP) position is enhanced. The discovery of a reliable starting point that fulfills critical criteria for a plausible medicinal chemistry project is demonstrated in this prospective study.
Plasmaféresis terapéutica. Análisis de 2 a?os en el Instituto de Hematología e Inmunología
Fernández-Delgado,Norma; Forrellat-Barrios,Mariela; González-Suárez,Tania; Salgado-Arocena,Odalys;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2012,
Abstract: therapeutic plasma exchange is an extracorporeal procedure in which, after removing the whole blood, plasma is separated from other cellular components with the aim of reducing or eliminating pathological substances involved in the pathophysiology of the disease. today, apheresis procedure is the most frequently used in clinical medicine. in this paper, we mention fundamental advances in their indications, methods and replacement solutions. we evaluate indications of plasmapheresis, which were performed at the institute of hematology and immunology from january 2009 to december 2010. there were evaluated 295 plasmapheresis performed in 35 patients with 9 diseases or conditions associated with them. 60 % matched class i of the american association of apheresis (asfa) and this procedure was effective in 88.6 % of cases. the exchange volume ranged from 750 to 2 400 ml and the most common replacement solution was saline solution, followed by fresh frozen plasma and albumin. 4.06 % of these patients had some adverse reactions related to the proceeding, but these were mild and easily controlled. plasmapheresis is a procedure with enormous therapeutic benefits when used in diseases, which have proven its effectiveness.
Plasmaféresis terapéutica. Análisis de 2 a os en el Instituto de Hematología e Inmunología Therapeutic plasmapheresis. Two-year analysis at the Institute of Hematology and Immunology
Norma Fernández-Delgado,Mariela Forrellat-Barrios,Tania González-Suárez,Odalys Salgado-Arocena
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2012,
Abstract: La plasmaféresis terapéutica es un procedimiento extracorpóreo en el que, a partir de la extracción de sangre total, se separa el plasma del resto de los componentes celulares con el objetivo de disminuir o eliminar determinadas sustancias patológicas que intervienen en la fisiopatología de la enfermedad. En la actualidad, es el procedimiento de aféresis más frecuentemente usado en la medicina clínica. Se mencionan los avances fundamentales en sus indicaciones, métodos y soluciones de reposición, y se evalúan las indicaciones de plasmaféresis realizadas en el Instituto de Hematología e Inmunología en el período comprendido entre enero de 2009 y diciembre de 2010. Se evaluaron 295 plasmaféresis realizadas a 35 pacientes con 9 enfermedades o condiciones asociadas con ellas; el 60 % correspondieron con la categoría I de la Asociación Americana de Aféresis (ASFA) y el proceder mostró efectividad en el 88,6 % de los casos. El volumen de recambio osciló entre 750 y 2 400 mL y la solución de reposición más empleada fue la solución salina, seguida del plasma fresco congelado y la albúmina. En el 4,06 % de los casos se evidenció alguna reacción adversa relacionada con el proceder, pero estas fueron leves y de fácil control. La plasmaféresis es un proceder con enormes beneficios terapéuticos cuando se utiliza en enfermedades en las que ha demostrado su eficacia. Therapeutic plasma exchange is an extracorporeal procedure in which, after removing the whole blood, plasma is separated from other cellular components with the aim of reducing or eliminating pathological substances involved in the pathophysiology of the disease. Today, apheresis procedure is the most frequently used in clinical medicine. In this paper, we mention fundamental advances in their indications, methods and replacement solutions. We evaluate indications of plasmapheresis, which were performed at the Institute of Hematology and Immunology from January 2009 to December 2010. There were evaluated 295 plasmapheresis performed in 35 patients with 9 diseases or conditions associated with them. 60 % matched Class I of the American Association of Apheresis (ASFA) and this procedure was effective in 88.6 % of cases. The exchange volume ranged from 750 to 2 400 mL and the most common replacement solution was saline solution, followed by fresh frozen plasma and albumin. 4.06 % of these patients had some adverse reactions related to the proceeding, but these were mild and easily controlled. Plasmapheresis is a procedure with enormous therapeutic benefits when used in diseases, which have proven its effecti
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