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Xenodiagnostico con Lutzomyia youngi en casos venezolanos de leishmaniasis cutaned por Leishmania braziliensis
Rojas, Elina;Scorja, Jose V.;
Memórias do Instituto Oswaldo Cruz , 1989, DOI: 10.1590/S0074-02761989000100006
Abstract: eight patients infected with leishmania braziliensis were used for xenodiagnosis with lutzomtyia youngi, before and after specific antileishmanial treatment with "glucantime" and "gabbromycin". all of them infected sandflies fed on the borders of the skin lesions before the treatment, suggesting that infected persons might act as reservoirs of infection for an indoor-bitting sandfly species. the negative results obtained by xenodiagnosis carried out after specific treatment of the same individuals indicated cure of the patients, and a reduction of risk for further intradomiciliary transmission.
Leishmania braziliensis: aislamiento de lesiones por inoculación de hámsteres con o sin adición de lisado de glándulas salivares de Lutzomyia youngi
Rojas,Elina; Scorza,José V.;
Revista de Saúde Pública , 1995, DOI: 10.1590/S0034-89101995000100002
Abstract: homogenized biopsy tissue from the cutaneous leishmaniasis lesions of 50 patients from trujillo, venezuela, were inoculated subcutaneously into the tarsi of male hamsters. homogenized tissue either alone or mixed with salivary gland lysates of lutzomyia youngi were used for inoculation. homogenized tissue alone yielded 58.5% of infections with a mean of twelve weeks for prepatency, while those mixed with sandfly lysate resulted in 92% of infections with a mean prepatency of three weeks.
Presencia de nectomonadas metaciclicas de L. pifanoi en la hipofaringe de Lutzomyia youngi y la ingestion de azúcares
Rojas,Elina; Scorza,José V.;
Revista de Saúde Pública , 1996, DOI: 10.1590/S0034-89101996000300006
Abstract: the presence of metacyclical promastigotes of leishmania pifanoi in the hypopharyngeal duct of lutzomyia youngi is reported. the insects were experimentally infected by engorgement on the tarsal lesions of hamsters. the metacyclics, whose morphology is illustrated, appeared in the hypopharynx 5 to 9 days after engorgement; they were more frequently found in the insects fed on unrefined commercial sugar. they role of amino derivates of glucose and galactose, as well as of amino acids in the development and migration of metacyclics, is investigated.
Leishmania braziliensis: aislamiento de lesiones por inoculación de hámsteres con o sin adición de lisado de glándulas salivares de Lutzomyia youngi
Rojas Elina,Scorza José V.
Revista de Saúde Pública , 1995,
Abstract: Homogeneizados de biopsias de lesiones cutáneas de 50 casos de leishmaniasis tegumentaria de Trujillo, Venezuela, han sido inoculados en hámsteres machos. Se ha comparado la infectvidad de Leishamania braziliensis, de homogeneizados simples, con la de los mezclados con lisado de glándula salival de Lutzomyia youngi, registrandose un 58,5% de infecciones para una media de 12 semanas de prepatencia con los homogeneizados simples, contra 92% de infecciones con una media de 3 semanas de prepatencia, cuando cada uno de los inóculos de homogeneizado se mezcló con lisado equivalente al de una glándula salival de flebótomo.
Presencia de nectomonadas metaciclicas de L. pifanoi en la hipofaringe de Lutzomyia youngi y la ingestion de azúcares
Rojas Elina,Scorza José V.
Revista de Saúde Pública , 1996,
Abstract: Se evidencia la presencia de promastigotos metacíclicos de Leishmania pifanoi en el conducto hipofaríngeo de Lutzomyia youngi infectados experimentalmente por ingurgitación sobre lesiones tarsales de hámsteres. La aparición de metacíclicos en la hipofaringe, cuya morfología se ilustra, ocurre entre los 5 y 9 días de desarrollo postprandial y es más frecuente en insectos alimentados con sacarosa comercial no refinada. Se investiga el papel de derivados aminados de glucosa y galactosa y también de aminoácidos, en la promoción y migración de los metacíclicos.
Excreción de promastigotos de Leishmania pifanoi por Lutzomyia youngi experimentalmente infectada
Rojas,Elina; Scorza,Jose V.; Espinoza,Alba;
Revista de Saúde Pública , 1995, DOI: 10.1590/S0034-89101995000600011
Abstract: the increase in the promastigotes population of leishmania pifanoi in lutzomyia youngi experimentally infected and kept on 50% sacarose under constant conditions of temperature and humidity is described. two stages in the differentation and growth of the parasites are recognised between two and twenty-four hours after meals. the pleomorphic differentiation of the amastigotes in short promastigotes which multiply by binary division for 60 hours, when the rupture of the peritrophic membrane occurs, takes place within 48 hours. the second stage occurs between 72 and 96 hours when some of the parasites migrate to the esophagic valve and the rest of the free parasites are excreted in fecal drops as large, active promastigotes. the first drops excreted react positively to glucose or contain crystals of urate. the excess promastigotes of the second stage of development are eliminated in the last excretions and react positively to the hemoscreen and biuret tests for total proteins as also for glucose and account for 82% of the of drops excreted. the excretion of parasites by lu. youngi is a normal stage in the growth of l. pifanoi into a vector.
Excreción de promastigotos de Leishmania pifanoi por Lutzomyia youngi experimentalmente infectada
Rojas Elina,Scorza Jose V.,Espinoza Alba
Revista de Saúde Pública , 1995,
Abstract: Se describe el desarrollo poblacional promastigótico de Leishmania pifanoi en Lutzomyia youngi experimentalmente infectada y mantenida con sacarosa al 50% bajo condiciones constantes de temperatura y humedad. Se reconocen dos etapas para la diferenciación y el crecimiento de los parásitos entre las dos y ciento veinte horas postprandiales. Hasta 48 horas tiene lugar la diferenciación pleomórfica de amastigotos en promastigotos cortos, que se multiplican por división binaria hasta las 60 horas, cuando ocurre la ruptura de la membrana peritrófica. La segunda etapa tiene lugar entre las 72 y 96 horas cuando algunos parásitos migran hacia la válvula esofágica y los demás parásitos libres son excretados en gotitas fecales como promastigotos grandes y activos. Las primeras gotitas excretadas dan reacción positiva a glucosa o contienen cristales de urato. El exceso de promastigotos de la segunda fase de desarrollo es eliminado en las últimas excretas que dan reacción positiva con las pruebas Hemoscreen y Biuret para proteínas totales y también para glucosa, y constituyen el 82% del total de gotas excretadas. La excreción de parásitos por Lu. youngi es fase normal del desarrollo de L. pifanoi en un vector.
Infectividad del perro (Canis familiaris) para Lutzomyia youngi en Trujillo, Venezuela
Hernández,Dalila; Rojas,Elina; Scorza,José Vicente; Jorquera,Alicia;
Biomédica , 2006,
Abstract: introduction. in trujillo, venezuela the prevalence for american tegumentary leishmaniasis (atl) is 38 per 100.000 inhabitants. objective. in a periurban, rural settlement of the capital city trujillo, we studied the potential capability of the domestic dog (canis familiaris) as a source of infection for lutzomyia youngi, a phlebotomine sand fly species abundant in the study area and whose domestic vectorial activity has been proven. materials and methods. dogs with dermal lesions suggestive of atl and parasitological confirmation of infection, were selected for xenodiagnosis by allowing sylvatic phlebotomines from a atl free area, to feed ad libitum over each animal′s entire body surface. the insects′ intestinal tracts were dissected 5 days after the blood meal in order to look for flagellate forms. when these were found, parasitological identification was performed by the multiplex-pcr technique. results. four hundred and fifty five sand flies engorged over two dogs in three different assays; promastigotes were found in 4 (0.88%) of the specimens on only one occasion. pcr identified dna of the leishmania viannia subgenus. conclusion. the household dog has the potential of being a domestic risk factor in the atl transmission cycle.
CAPACITACIóN COMUNITARIA EN HERRAMIENTAS DE DIFUSIóN ANTIDENGUE EN EL CAMBALACHE, TRUJILLO, VENEZUELA
Gutiérrez V, Olga,Naranjo de P.,Rojas Elina
Salud, Arte y Cuidado , 2012,
Abstract: Dengue is an endemic disease that is increasing in Venezuela, meaning a public health problem, the best method to treat it is to prevent. Creating a community training component aimed at the diffusion, through health education in the population sector ¨the cambalache¨Pampanito municipality of Trujillo state, a descriptive study was conducted with a purposive sample of 24 volunteer families from a population of 40 families belonging to the sector with the interviews and participant observation data were oral antiDengue us to understandthe culture in the population. As data collection instruments, we use the interview guide and diary, the total number of individuals who formed the sample was successfully observed that the community has 100% knowledge, 80% training and 20% organization in the disease control endemic and epidemic Dengue. Educational activities were conducted with different families microradiales sector editing, games, puzzles, existing material. The population refers to the care given by health agencies and education is poor and remains affected by the vector. We conclude that training and training in antidengue dissemination tools were well accepted by the community. The implementation of information days about Dengue was the basis for the conduct of workshops and development of micro, wallets and other tools for community member’s newspapers that allowed the reorganization of the community in which it was observed an epistemological problem of health.
Reducción de flora contaminante en alimentos mediante tratamiento con microondas
Montilla M,Miriam del C; Scorza B,José V; Rojas M,Elina M;
Revista del Instituto Nacional de Higiene Rafael Rangel , 2010,
Abstract: food that is served by mobile stall and consumed on the street is a nutritional alternative for workers and students but, sometimes, the bacterial contamination of this ?fast food? can be a diseasis transmitted by food to consumer. in this study of the effect of microwave ovens on the contamination of food, seventy-seven examples of ?fast food? were collected from mobile stalls (twenty-one hot-dogs, thirty-two empanadas and twenty-for arepas). the sample were taken in aseptic conditions and transferred to the laboratory in cold storage. each sample was divided into two parts. one part was irradiated with microwaves for one minute and the subjected to a bacteriological analysis. the other was not subjected to the radiation treatment. fecal contamination was confirmed in 60% of the samples examined; the one minute exposure to microwaves reduced the levels of bacterial coliforms by 85.4%. the results revealed the destruction of bacteria in 90% of the hot-dogs, in 40% of the empanadas and in 66.6% of de arepas that were diagno-sed as positive. the effect of microwaves proved to be an effective technique in reducing the levels of bacterial conta-minants in food-stuffs.
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