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Search Results: 1 - 10 of 14342 matches for " Rodrigo Josemar Seminoti Jacques "
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Eucalyptus essential oil action on mycorrhizal colonization and the establishment of Eucalyptus grandis in soil contaminated by copper A o do óleo essencial de eucalipto na micorriza o e no estabelecimento de Eucalyptus grandis em solo contaminado por cobre
Ricardo Bemfica Steffen,Zaida Inês Antoniolli,Gerusa Pauli Kist Steffen,Rodrigo Josemar Seminoti Jacques
Pesquisa Florestal Brasileira , 2011, DOI: 10.4336/2012.pfb.67.245
Abstract: The use of eucalyptus essential oil can optimize the growth of ectomycorrhizal isolates, which aid the establishment of forest species in soils contaminated by copper. The study aimed to determine the best application of eucalyptus essential oil in the formation of mycorrhizal seedlings of Eucalyptus grandis and its influence on the establishment of these seedlings in soil contaminated by copper. It was used the ectomycorrhizal fungi (fECM) Pisolithus microcarpus. The application forms of the essential oil were evaluated in a greenhouse, by means of six treatments. Subsequently, the seedlings were transplanted and evaluated in soil contaminated by copper, greenhouse and field. It was determined height, diameter, fresh and dry weight of shoots and roots, copper content in plant, colonization and survival in the field. The essential oil of E. grandis supports the growth of mycorrhizal of eucalyptus seedlings, particularly when applied to the substrate. The application of essential oil increases the colonization, promoting growth and survival of seedlings under greenhouse conditions and field. doi: 10.4336/2011.pfb.31.67.245 A utiliza o do óleo essencial de eucalipto pode otimizar o crescimento de isolados ectomicorrízicos, os quais auxiliam o estabelecimento de essências florestais em solos contaminados por cobre. O trabalho teve por objetivo determinar a melhor forma de aplica o do óleo essencial de eucalipto na forma o de mudas de Eucalyptus grandis micorrizadas e sua influência no estabelecimento dessas mudas em solo contaminado por cobre. Utilizou-se o fungo ectomicorrízico (fECM) Pisolithus microcarpus. As formas de aplica o do óleo essencial foram avaliadas em casa de vegeta o, por meio de seis tratamentos. Posteriormente, as mudas foram transplantadas e avaliadas em solo contaminado por cobre, em condi es de casa de vegeta o e campo. Determinou-se altura, diametro, massa fresca e seca da parte aérea e raízes, teor de cobre na planta, coloniza o micorrízica e sobrevivência no campo. O óleo essencial de E. grandis favorece o crescimento de mudas de eucalipto micorrizadas, principalmente quando aplicado diretamente no substrato. A aplica o do óleo essencial aumenta a coloniza o micorrízica, favorecendo o crescimento e a sobrevivência das mudas em condi es de casa de vegeta o e campo.doi: 10.4336/2011.pfb.31.67.245
Efeito do óleo essencial de Eucalyptus grandis no crescimento de isolados de fungos ectomicorrízicos em diferentes concentra es de cobre, zinco e níquel Essential oil of Eucalyptus grandis effect on the growth of ectomycorrhizal isolates in different copper, zinc and nickel concentrations
Ricardo Bemfica Steffen,Zaida Inês Antoniolli,Gerusa Pauli Kist Steffen,Rodrigo Josemar Seminoti Jacques
Pesquisa Florestal Brasileira , 2011, DOI: 10.4336/2012.pfb.67.227
Abstract: Os metabólitos secundários bioativos de algumas plantas s o capazes de estimular o crescimento dos fungos ectomicorrízicos. Quando em associa o com plantas, esses fungos proporcionam as mesmas condi es de se desenvolverem em ambientes contaminados por metais pesados. Avaliou-se o efeito da adi o de óleo essencial de Eucalyptus grandis no crescimento de isolados ectomicorrízicos na presen a de cobre, zinco e níquel. Os fungos ectomicorrízicos Pisolithus microcarpus (UFSC Pt 116) e Pisolithus sp. (UFSC Pt 24) foram incubados em meio de cultura líquido na presen a de concentra es crescentes de cobre, zinco e níquel e de óleo essencial de Eucalyptus grandis na concentra o de 20 μL L-1. Após período de incuba o de 25 dias, avaliou-se a massa seca do micélio e a concentra o que inibiu o crescimento fúngico em 50%. Nas concentra es de cobre, zinco e níquel superiores a 3,94, 1,57 e 0,85 mmol L-1, respectivamente, n o foi verificado aumento no crescimento dos isolados ectomicorrízicos avaliados pela adi o do óleo essencial. A presen a do óleo essencial de E. grandis na concentra o de 20 μL L-1 em meio de cultura líquido aumentou a tolerancia dos isolados ectomicorrízicos UFSC Pt 116 e UFSC Pt 24 aos metais pesados cobre, zinco e níquel. doi: 10.4336/2011.pfb.31.67.227 The bioactive secondary metabolites of some plants are capable of stimulating the growth of ectomycorrhizal fungi. Combined with plants, these fungi provide the same conditions to grow in environments contaminated by heavy metals. We evaluated the effect of adding essential oil of Eucalyptus grandis in the growth of ectomycorrhizal isolates in the presence of copper, zinc and nickel. The ectomycorrhizal Pisolithus microcarpus (UFSC Pt 116) and Pisolithus sp. (UFSC Pt 24) were incubated in liquid culture medium in the presence of increasing concentrations of copper, zinc and nickel and essential oil of Eucalyptus grandis at a concentration of 20 μL L-1. After an incubation period of 25 days, we estimated the dry mass of mycelium and concentration that inhibited fungal growth by 50%. At concentrations of copper, zinc and nickel above of 3.94, 1.57 and 0.85 mmol L-1 respectively, no increase was observed in the growth of ectomycorrhizal isolates evaluated by the addition of essential oil. The presence of essential oil of E. grandis at a concentration of 20 μL L-1 in liquid culture medium increased the tolerance of ectomycorrhizal isolates UFSC Pt 116 and Pt 24 UFSC to the heavy metals copper, zinc and nickel. doi: 10.4336/2011.pfb.31.67.227
Essential oil influence in mycorrhizal colonization and growth seedlings of eucalyptus Influência do óleo essencial na micorriza o e no crescimento de mudas de eucalipto
Ricardo Bemfica Steffen,Zaida Inês Antoniolli,Gerusa Pauli Kist Steffen,Rodrigo Josemar Seminoti Jacques
Pesquisa Florestal Brasileira , 2011, DOI: 10.4336/2012.pfb.67.235
Abstract: The establishment of exotic forest species in Brazil may show dependence to ectomycorrhizal association, which increases the resistance of seedlings to stress after the initial planting in the field, favoring the maintenance of harshseedlings under adverse conditions. The study aimed to evaluate the effect of applying the eucalyptus essential oil in ectomycorrhizal colonization on growth of Eucalyptus grandis seedlings in greenhouse conditions. The treatments consisted of five concentrations of the Eucayptus grandis essential oil and two ectomycorrhizal isolates, in addition to the control treatment without inoculation. It was used a completely randomized design with eight repetitions. Ninety days after transplanting the eucalyptus seedlings were evaluated: height (cm), stem diameter (mm), dry massof shoots and roots (mg) and percentage of ectomycorrhizal colonization. The eucalyptus essential oil was efficient in stimulating ectomycorrhizal colonization of eucalyptus seedlings, resulting in significant increases in dry weight of shoots of mycorrhizal seedlings, showing doseresponse effect, depending on the ectomycorrhizal used. doi: 10.4336/2011.pfb.31.67.235 O estabelecimento das essências florestais exóticas pode apresentar dependência da associa o ectomicorrízica, a qual eleva a resistência das mudas aos estresses iniciais após o plantio no campo, favorecendo o desenvolvimento inicial das mudas sob condi es adversas. O trabalho objetivou avaliar o efeito da aplica o do óleo essencial de eucalipto na coloniza o ectomicorrízica e no crescimento de mudas de Eucalyptus grandis, em condi es de casa de vegeta o. Os tratamentos foram constituídos por cinco concentra es do óleo essencial de Eucayptus grandis e dois isolados ectomicorrízicos, além dos tratamentos controle sem inocula o. Utilizou-se delineamento experimental inteiramente casualizado com oito repeti es. Noventa dias após o transplante das plantulas de eucalipto para tubetes foram avaliados os parametros: altura da muda (cm), diametro do colo (mm), massa seca da parte aérea e raízes (mg) e percentual de coloniza o ectomicorrízica. O óleo essencial de eucalipto mostrou-se eficiente no estímulo à coloniza o ectomicorrízica das mudas de eucalipto, resultando em aumentos significativos na coloniza o e na massa fresca e massa seca da parte aérea das mudas micorrizadas, apresentando resposta de dose-efeito, dependendo do isolado ectomicorrízico utilizado. doi: 10.4336/2011.pfb.31.67.235
Biorremedia??o de um solo contaminado com antraceno sob diferentes condi??es físicas e químicas
Jacques, Rodrigo Josemar Seminoti;Silva, Kelly Justin da;Bento, Fátima Menezes;Camargo, Flávio Anastácio de Oliveira;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010000200009
Abstract: the anthracene, as well as the others polycyclic aromatic hydrocarbons (pah), can be removed from the soil by bioremediation, whose efficiency is limited under unfavorable physical and chemical conditions to the survival and activity of the microbial degraders. the objective of this study was to evaluate the influence of ph, water content, and nitrogen, phosphorus, iron and sulfur concentrations in the bioremediation of a soil contaminated with anthracene. samples of a sandy soil were contaminated in laboratory with anthracene (500mg kg-1) and the mineralization was evaluated by respirometry. the highest anthracene mineralization was verified in the soil with the highest water content and ph value studied. the addition of 100kg ha-1 nitrogen in the soil and the consequent reduction of cpah-n ratio to values lesser than 120:17 reduced anthracene mineralization. the increase of phosphorus iron and sulfur availability and wide cpah-p (120:1 to 120:22) ratios in the soil did not influence anthracene mineralization. the ph correction and appropriate water supply made possible the bioremediation of the soil polluted with anthracene in a short period of time.
Biorremedia??o de solos contaminados com hidrocarbonetos aromáticos policíclicos
Jacques, Rodrigo Josemar Seminoti;Bento, Fátima Menezes;Antoniolli, Zaida Inês;Camargo, Flávio Anastácio de Oliveira;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000400049
Abstract: polycyclic aromatic hydrocarbons (pahs) are mutagenic and carcinogenic compounds to the humans and animals, released through the environment by anthropogenic activities related to the extraction, transport, refine, transformation and use of the petroleum and its derivatives. most of the soils microorganisms do not possess the capacity to degrade them, which results in its accumulation in the atmosphere and contamination of the ecosystems. a strategy for pahs elimination from the soil is through the bioremediation, where microorganisms having capacity to metabolize these compounds will transform them in inert substances, co2 and water. however, this biotechnology can be limited by the lack of specific hap microbial-degraders in soil, by unfavorable environmental conditions to these microorganisms or by the low bioavailability of those contaminants to the microorganisms. to overcome these limitations and to promote an efficient removal of the pollutants to the atmosphere, several bioremediation techniques were developed as passive bioremediation, bioaugmentation, biostimulation, phytoremediation, landfarming, composting and bioreactors. this revision aims at discussing microbial metabolism of pahs, present the main chemical and physical factors that influence the survival and the activity of these microorganisms and to show the bioremediation techniques that are being used now for the pahs removal in soil.
Sensibilidade de estirpes de Bradyrhizobium ao glyphosate
Jacques, Rodrigo Josemar Seminoti;Procópio, Sérgio de Oliveira;Santos, José Barbosa dos;Kasuya, Maria Catarina Megumi;Silva, Ant?nio Alberto da;
Revista Ceres , 2010, DOI: 10.1590/S0034-737X2010000100006
Abstract: glyphosate applications to glyphosate-resistant soybean may impair symbiosis with bradyrhizobium, a symbiotic bacterium. this study evaluated the sensitivity to glyphosate of three bradyrhizobium strains used commercially in soybean crop in brazil. the concentrations (0.0; 5.4; 10.8; 21.6 e 43.2 μg l-1) of the active ingredient glyphosate [n-(phosphonomethyl)glycine] were added to ym liquid medium for the growth of one strain of b. japonicum (semia 5079) and two strains of b. elkanni (semia 5019 and semia 587) inoculated as pure culture. the herbicide effect on the growth of the tested strains was assessed by optical density and growth curves were established for each strain. reduction in growth of strains in the lowest glyphosate concentration were 18% for semia 5079, 29% for semia 5019 and 35% for semia 587. in general, the higher the concentration of the herbicide in the culture medium, the greater the growth inhibition. the strains showed differential sensitivity only at the lowest concentrations of glyphosate, allowing the determination of the following order of sensitivity: semia 587>semia 5019>semia 5079. this differential sensitivity is dependent on the herbicide concentration, since in the presence of 43.2 μg l-1 all strains severely reduced growth, not showing difference among them.
Soil-Borne Bacterial Structure and Diversity Does Not Reflect Community Activity in Pampa Biome
Manoeli Lupatini, Afnan Khalil Ahmad Suleiman, Rodrigo Josemar Seminoti Jacques, Zaida Inês Antoniolli, Eiko Eurya Kuramae, Flávio Anastácio de Oliveira Camargo, Luiz Fernando Würdig Roesch
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076465
Abstract: The Pampa biome is considered one of the main hotspots of the world’s biodiversity and it is estimated that half of its original vegetation was removed and converted to agricultural land and tree plantations. Although an increasing amount of knowledge is being assembled regarding the response of soil bacterial communities to land use change, to the associated plant community and to soil properties, our understanding about how these interactions affect the microbial community from the Brazilian Pampa is still poor and incomplete. In this study, we hypothesized that the same soil type from the same geographic region but under distinct land use present dissimilar soil bacterial communities. To test this hypothesis, we assessed the soil bacterial communities from four land-uses within the same soil type by 454-pyrosequencing of 16S rRNA gene and by soil microbial activity analyzes. We found that the same soil type under different land uses harbor similar (but not equal) bacterial communities and the differences were controlled by many microbial taxa. No differences regarding diversity and richness between natural areas and areas under anthropogenic disturbance were detected. However, the measures of microbial activity did not converge with the 16S rRNA data supporting the idea that the coupling between functioning and composition of bacterial communities is not necessarily correlated.
On the decomposition of k-valued rational relations
Jacques Sakarovitch,Rodrigo De Souza
Mathematics , 2008,
Abstract: We give a new, and hopefully more easily understandable, structural proof of the decomposition of a $k$-valued transducer into $k$ unambiguous functional ones, a result established by A. Weber in 1996. Our construction is based on a lexicographic ordering of computations of automata and on two coverings that can be build by means of this ordering. The complexity of the construction, measured as the number of states of the transducers involved in the decomposition, improves the original one by one exponential. Moreover, this method allows further generalisation that solves the problem of decomposition of rational relations with bounded length-degree, which was left open in Weber's paper.
Disponibilidade de nitrogênio e produtividade de milho e trigo com diferentes métodos de aduba??o nitrogenada no sistema plantio direto
Da Ros, Clovis Orlando;Salet, Roberto Luiz;Porn, Rodrigo Luiz;Machado, Josemar Nerí Corrêa;
Ciência Rural , 2003, DOI: 10.1590/S0103-84782003000500002
Abstract: the aim of this experiment was to evaluate the n availability for corn and wheat with different methods of n fertilization. the study was carried out at university of cruz alta, state of rio grande do sul, brazil, in an oxissol under six years of no-tillage. a completely randomized block design with four repetitions was used. combinations of different times and rates of n application were studied. the times were: application before seeding, at seeding and topdressing. the rates of n for corn were zero, 60 and 90kg ha-1 and for wheat zero, 30 and 60kg ha-1. the amount of soil mineral n decreased quickly after application of n fertilization. the soil′s mineral n content was related to the rates and time of application. the n application time, considering the same rates, splitted or not, didn't influence dry matter production, n accumulation and grain yield. these parameters increase with the rate of n application. the splitting of the n fertilization, part at the seeding time and part as topdressing, is the safest way to minimize the n losses and to maximize the use of n by corn and wheat plants.
Disponibilidade de nitrogênio e produtividade de milho e trigo com diferentes métodos de aduba o nitrogenada no sistema plantio direto
Da Ros Clovis Orlando,Salet Roberto Luiz,Porn Rodrigo Luiz,Machado Josemar Nerí Corrêa
Ciência Rural , 2003,
Abstract: Este trabalho teve como objetivo avaliar a disponibilidade de N para as culturas de milho e trigo com diferentes métodos de aduba o nitrogenada. O estudo foi realizado na Universidade de Cruz Alta, em Latossolo Vermelho Distrófico argiloso, com seis anos no sistema plantio direto. O delineamento experimental foi de blocos ao acaso com quatro repeti es. Os tratamentos foram constituídos por combina es de diferentes épocas e doses de aplica o de N. As épocas foram: em pré-semeadura, na semeadura das culturas e em cobertura. As doses foram: zero, 30 e 90kg ha-1 de N para a cultura do milho e zero, 30 e 60kg ha-1 de N para a cultura do trigo. Os valores de N mineral no solo foram afetados pelas doses e épocas das aplica es. A quantidade de N mineral no solo proveniente da aduba o nitrogenada na cultura do milho diminuiu rapidamente após a aplica o. A época de aplica o de N, considerando a mesma dose, seja parcelada ou n o, n o influenciou a produ o de matéria seca, N acumulado e produtividade de gr os. Foi observada varia o somente entre as doses totais aplicadas, independentemente das épocas. As maiores produtividades de gr os foram obtidas com a utiliza o das doses mais elevadas de N. Em termos gerais, observou-se que a aplica o parcelada de N, na semeadura e em cobertura, conferiu as melhores produtividades, possivelmente devido a menores perdas de N e a maximiza o da absor o de N pelas plantas de milho e de trigo.
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