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Search Results: 1 - 10 of 160352 matches for " Rodolfo H. Campos "
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Stability of the Resistance to the Thiosemicarbazone Derived from 5,6-Dimethoxy-1-Indanone, a Non-Nucleoside Polymerase Inhibitor of Bovine Viral Diarrhea Virus
Eliana F. Castro, Rodolfo H. Campos, Lucía V. Cavallaro
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0100528
Abstract: Bovine viral diarrhea virus (BVDV) is the prototype Pestivirus. BVDV infection is distributed worldwide and causes serious problems for the livestock industry. The thiosemicarbazone of 5,6-dimethoxy-1-indanone (TSC) is a non-nucleoside polymerase inhibitor (NNI) of BVDV. All TSC-resistant BVDV variants (BVDV-TSCr T1–5) present an N264D mutation in the NS5B gene (RdRp) whereas the variant BVDV-TSCr T1 also presents an NS5B A392E mutation. In the present study, we carried out twenty passages of BVDV-TSCr T1–5 in MDBK cells in the absence of TSC to evaluate the stability of the resistance. The viral populations obtained (BVDV R1–5) remained resistant to the antiviral compound and conserved the mutations in NS5B associated with this phenotype. Along the passages, BVDV R2, R3 and R5 presented a delay in the production of cytopathic effect that correlated with a decrease in cell apoptosis and intracellular accumulation of viral RNA. The complete genome sequences that encode for NS2 to NS5B, Npro and Erns were analyzed. Additional mutations were detected in the NS5B of BVDV R1, R3 and R4. In both BVDV R2 and R3, most of the mutations found were localized in NS5A, whereas in BVDV R5, the only mutation fixed was NS5A V177A. These results suggest that mutations in NS5A could alter BVDV cytopathogenicity. In conclusion, the stability of the resistance to TSC may be due to the fixation of different compensatory mutations in each BVDV-TSCr. During their replication in a TSC-free medium, some virus populations presented a kind of interaction with the host cell that resembled a persistent infection: decreased cytopathogenicity and viral genome synthesis. This is the first report on the stability of antiviral resistance and on the evolution of NNI-resistant BVDV variants. The results obtained for BVDV-TSCr could also be applied for other NNIs.
Molecular Survey of Hepatitis C Virus in the Touristic City of Mar Del Plata, Argentina
Andrés C. A. Culasso, Mercedes Elizalde, Rodolfo H. Campos, Luciana Barbini
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044757
Abstract: The global epidemiology of Hepatitis C Virus (HCV) may be roughly described by two groups of genotypes: the worldwide distributed ones (subtypes 1a, 1b, 2a and 3a, among others) and the endemic ones (subtypes 4a, 5a, 6a, among others). Epidemiological and population dynamic studies of the worldwide distributed genotypes have shown that subtypes 1a and 3a are common among intravenous drug users (IDUs) and that they are also in expansion in some countries. The molecular survey of HCV provides some clues about the epidemiological status of the infections in a local scale and the phylogenetic and demographic reconstruction analyses complement this study by inferring whether the infections of certain subtypes are in a steady state or expanding. Here, a molecular survey of the HCV variants that circulate in the touristic city of Mar del Plata (Buenos Aires, Argentina) was performed in samples obtained from 42 patients. The subtypes detected were 1a (32 patients), 3a (8 patients) and 1b (2 patients). The demographic history of subtype 1a inferred using the sequence data showed an exponential growth in the 1990′s. The period of viral expansion was delayed compared with that observed for the same genotype in other countries where the transmission was associated with IDUs. Also, the phylogeographic analysis of HCV-1a showed a statistically significant association between the location of the samples and the phylogeny, which may be the result of the local transmission of HCV in the city. The molecular analysis helped in the description of the complex epidemiological context of a touristic city, and pointed out that some sanitary measures should be taken in order to reduce the transmission of HCV (and maybe of HIV) among IDUs.
High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis
Marcelo D. Golemba,Federico A. Di Lello,Fernando Bessone,Fabian Fay,Silvina Benetti,Leandro R. Jones,Rodolfo H. Campos
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008751
Abstract: Previous studies in Argentina have documented a general prevalence of Hepatitis C Virus (HCV) infection close to 2%. In addition, a high prevalence of HCV has been recently reported in different Argentinean small rural communities. In this work, we performed a study aimed at analyzing the origins and diversification patterns of an HCV outbreak in Wheelwright, a small rural town located in Santa Fe province (Argentina).
Eating disorders: need for a broader assessment Transtornos alimentares: necessidade de uma avalia o mais ampla
Rodolfo Nunes Campos
Trends in Psychiatry and Psychotherapy , 2012, DOI: 10.1590/s2237-60892012000100002
LICAVAL: combination therapy in acute and maintenance treatment of bipolar disorder
Rodolfo N Campos, Luis F Costa, Danielle S Bio, Márcio G Soeiro de Souza, Carla RL Garcia, Frederico N Demétrio, Doris H Moreno, Ricardo A Moreno
Trials , 2010, DOI: 10.1186/1745-6215-11-72
Abstract: LICAVAL is a single site, parallel group, randomized, outcome assessor blinded trial. BD I patients according to the DSM-IV-TR, in depressive, manic,/hypomanic or mixed episode, aged 18 to 35 years are eligible. After the diagnostic assessments, the patients are allocated for one of the groups of treatment (lithium + valproic acid or lithium + carbamazepine). Patients will be followed up for 8 weeks in phase I (acute treatment), 6 months in phase II (continuation treatment) and 12 months in phase III (maintenance treatment). Outcome assessors are blind to the treatment. The main outcome is the evaluation of changes in mean scores on CGI-BP-M between baseline and endpoint at the end of each phase of the study.LICAVAL is currently in progress, with patients in phase I, II or III. It will extended until august 2012.Trials comparing specific treatments efficacy in BD (head to head) can show relevant information in clinical practice. Long term treatment is an issue of great important and should be evaluated carefully in more studies as long as BD is a chronic disease.ClinicalTrials.gov Identifier: NCT00976794Bipolar Disorder (BD) treatment is a topic in evolution as long as the understanding of the clinical features, and possible pathophysiology, still progress. Due to multivariate factors associated with its cause and the variability of clinical presentations it's hard to determine a specific treatment with best outcome (efficacy and tolerability). Some difficulties in the BD treatment include: delay in diagnosis, high levels of comorbidity, frequent treatment nonadherence and high risk of relapse/recurrence (mainly in the presence of residual symptoms) [1].Most treatments focus on acute phase and the measure of response as a reduction in symptoms of at least 50% from baseline. In fact, a number of patients who responded to treatment continue to experience significant subsyndromic symptoms. A small number of studies reported remission rates, which mean at least 2 months
Human papillomavirus (HPV) detection and Papanicolaou cytology in low-resource women in Posadas city, Misiones, Argentina
Badano,Inés; Pedrozo,René W; Ruíz Díaz,Laura S; Galuppo,Juan A; Picconi,María A; Campos,Rodolfo H; Liotta,Domingo J;
Revista argentina de microbiolog?-a , 2011,
Abstract: the objective of this study was to determine the prevalence of hpv infection and cervical lesions present in women who attended a health center in a low-resource area of the city of posadas, misiones, argentina. cervical cell samples (n = 163) were processed for papanicolaou cytology and hpv-pcr tests. socio-cultural risk factors were estimated using the odds ratio (or, ci 95 %). cervical lesions were detected in 14.7 % of women. the general prevalence of hpv infection was of 38 %. the most common types among the total population were hpv-16 (9.8 %) and hpv-33 (9.3 %). hpv-16 was detected in association with 29.2 % and 6.5 % of women with and without cervical lesions, respectively, the or being 5.3 (1.8-15.8). risk factors for hpv-16 infection were a smoking habit and a history of previous sexually-transmitted diseases. these data are important for the implementation of prevention programs, including an appropriate introduction of vaccination and the baseline for virological surveillance in the vaccine era.
Hepatitis C Virus Diversification in Argentina: Comparative Analysis between the Large City of Buenos Aires and the Small Rural Town of O'Brien
Marcelo D. Golemba, Andrés C. A. Culasso, Federico G. Villamil, Patricia Bare, Adrián Gadano, Ezequiel Ridruejo, Alfredo Martinez, Federico A. Di Lello, Rodolfo H. Campos
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084007
Abstract: Background The estimated prevalence of HCV infection in Argentina is around 2%. However, higher rates of infection have been described in population studies of small urban and rural communities. The aim of this work was to compare the origin and diversification of HCV-1b in samples from two different epidemiological scenarios: Buenos Aires, a large cosmopolitan city, and O'Brien, a small rural town with a high prevalence of HCV infection. Patients and Methods The E1/E2 and NS5B regions of the viral genome from 83 patients infected with HCV-1b were sequenced. Phylogenetic analysis and Bayesian Coalescent methods were used to study the origin and diversification of HCV-1b in both patient populations. Results Samples from Buenos Aires showed a polyphyletic behavior with a tMRCA around 1887–1900 and a time of spread of infection approximately 60 years ago. In contrast, samples from óBrien showed a monophyletic behavior with a tMRCA around 1950–1960 and a time of spread of infection more recent than in Buenos Aires, around 20–30 years ago. Conclusion Phylogenetic and coalescence analysis revealed a different behavior in the epidemiological histories of Buenos Aires and óBrien. HCV infection in Buenos Aires shows a polyphyletic behavior and an exponential growth in two phases, whereas that in O'Brien shows a monophyletic cluster and an exponential growth in one single step with a more recent tMRCA. The polyphyletic origin and the probability of encountering susceptible individuals in a large cosmopolitan city like Buenos Aires are in agreement with a longer period of expansion. In contrast, in less populated areas such as O'Brien, the chances of HCV transmission are strongly restricted. Furthermore, the monophyletic character and the most recent time of emergence suggest that different HCV-1b ancestors (variants) that were in expansion in Buenos Aires had the opportunity to colonize and expand in O’Brien.
Genetic History of Hepatitis C Virus in Venezuela: High Diversity and Long Time of Evolution of HCV Genotype 2
Maria Z. Sulbarán,Federico A. Di Lello,Yoneira Sulbarán,Clarisa Cosson,Carmen L. Loureiro,Héctor R. Rangel,Jean F. Cantaloube,Rodolfo H. Campos,Gonzalo Moratorio,Juan Cristina,Flor H. Pujol
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0014315
Abstract: The subtype diversity of the hepatitis C virus (HCV) genotypes is unknown in Venezuela.
Scalar and spin-dependent relativistic effects on magnetic properties calculated with four-component methods: the nuclear magnetic resonance parameters of the lead halides
Rodolfo H. Romero
Physics , 2008,
Abstract: The results of calculations of nuclear magnetic resonance (NMR) parameters for the lead halides is reported in this paper. The results are obtained by using four-component methods. The use of the nonrelativistic L\'evy-Leblond Hamiltonian along with the relativistic Dirac-Coulomb and spin-free ones allows us to discriminate scalar and spin-dependent effects on the parameters. It is found that the wide range of the lead NMR spectra and their large anisotropies are, mainly, due to spin-dependent effects on the paramagnetic term. Among the relativistic scalar corrections, the so-called spin-Zeeman kinetic-energy term turns out to be dominant. The reduced spin-spin coupling constants become proportional to the product of the atomic numbers of the coupled nuclei.
Phylodynamics of Hepatitis C Virus Subtype 2c in the Province of Córdoba, Argentina
Viviana E. Ré,Andrés C. A. Culasso,Silvia Mengarelli,Adrián A. Farías,Fabián Fay,María B. Pisano,Osvaldo Elbarcha,Marta S. Contigiani,Rodolfo H. Campos
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019471
Abstract: The Hepatitis C Virus Genotype 2 subtype 2c (HCV-2c) is detected as a low prevalence subtype in many countries, except in Southern Europe and Western Africa. The current epidemiology of HCV in Argentina, a low-prevalence country, shows the expected low prevalence for this subtype. However, this subtype is the most prevalent in the central province of Córdoba. Cruz del Eje (CdE), a small rural city of this province, shows a prevalence for HCV infections of 5%, being 90% of the samples classified as HCV-2c. In other locations of Córdoba Province (OLC) with lower prevalence for HCV, HCV-2c was recorded in about 50% of the samples. The phylogenetic analysis of samples from Córdoba Province consistently conformed a monophyletic group with HCV-2c sequences from all the countries where HCV-2c has been sequenced. The phylogeographic analysis showed an overall association between geographical traits and phylogeny, being these associations significant (α = 0.05) for Italy, France, Argentina (places other than Córdoba), Martinique, CdE and OLC. The coalescence analysis for samples from CdE, OLC and France yielded a Time for the Most Common Recent Ancestor of about 140 years, whereas its demographic reconstruction showed a “lag” phase in the viral population until 1880 and then an exponential growth until 1940. These results were also obtained when each geographical area was analyzed separately, suggesting that HCV-2c came into Córdoba province during the migration process, mainly from Europe, which is compatible with the history of Argentina of the early 20th century. This also suggests that the spread of HCV-2c occurred in Europe and South America almost simultaneously, possibly as a result of the advances in medicine technology of the first half of the 20th century.
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