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Search Results: 1 - 10 of 24410 matches for " Robson Fernando Missio "
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Desenvolvimento de mudas de pupunheira em Latossolo argiloso compactado artificialmente em subsuperfície
Bordin, Ivan;Missio, Robson Fernando;Casimiro, Evandro Luiz Nogarolli;Castro, Ana Maria Conte e;Fey, Emerson;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000100046
Abstract: this study was carried out to evaluate the development of peach palm (bactris gasipaes h.b.k.) seedlings submitted to artificiality subsurface compacted layers of a clayly texture eutroferric red latossol. the compacted soil was at the middle ring of the pvc pots. they were 20cm of diameter, divided in tree rings with 15; 3,5 and 10cm from the up side. the soil was submitted to four density levels (1,0; 1,13; 1,28 and 1,44kg dm-3), which determined the treatments. the evaluations were accomplished in four times (4, 6, 8 and 10 months). the increase of the soil density levels didn't influence in the development of the aerial part of the peach palm seedlings. independent of the studied time, the clayly latossol density levels, didn't prevent the peach palm roots penetration.
Genetic divergence among barley accessions from Ethiopia
Tesfahun Alemu Setotaw,Luiz Ant?nio dos Santos Dias,Robson Fernando Missio
Crop Breeding and Applied Biotechnology , 2010,
Abstract: The study was done with the objective of assessing the genetic diversity existing among Ethiopian and ICARDAbarley germplasm using multivariate data analyses. The experiment was conducted at Asasa and Ambo in Ethiopia, in 10 x 10simple lattices with two replications. To quantify the differentiation among genotypes canonical discriminant analysis, clusteringanalysis and Mahanalobis (D2) distance were used. The study indicated that the first two canonical variates explained 95%and 91% of the total variation at Asasa and Ambo, respectively. At both the locations, genotypes showed maximum differentiationon days to maturity, grain filling period, tiller per plant and spike per plant. Analysis of clustering grouped the 100 genotypesinto four cluster groups at Asasa and six clusters at Ambo. Ethiopian landraces and genotypes from ICARDA grouped in thesame cluster groups indicated the germplasm exchange between the Ethiopian and ICARDA barley breeding programs.
NOTE-Polymorphic information content of SSR markers for Coffea spp.
Robson Fernando Missio,Eveline Teixera Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2010,
Abstract: Thirty-three coffee SSR primers from enriched genomic library with (GT)15 and (AGG)10 repeats were analyzedin 24 coffee tree accessions. Twenty-two primers were polymorphic among accessions; the number of alleles ranged from 2 to13, with the mean number of 5.1 alleles per primer. PIC values ranged from 0.08 to 0.79. The highest mean PIC values werefound for C. canephora (0.46), and the lowest values for C. arabica (0.22) and triploids (0.22) accessions. The polymorphicSSR markers used in this study were useful for genetic fingerprinting in the coffee tree, especially in the C. canephora and theleaf rust resistant arabica cultivars.
Development and validation of SSR markers for Coffea arabica L.
Robson Fernando Missio,Eveline Teixeira Caixeta,Eunize Maciel Zambolim,Laércio Zambolim
Crop Breeding and Applied Biotechnology , 2009,
Abstract: With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries withprobes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences.Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were morefrequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1%of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSRmarkers were validated for further genetic studies of C. arabica.
Desenvolvimento de mudas de pupunheira em Latossolo argiloso compactado artificialmente em subsuperfície
Bordin Ivan,Missio Robson Fernando,Casimiro Evandro Luiz Nogarolli,Castro Ana Maria Conte e
Ciência Rural , 2006,
Abstract: O trabalho teve como objetivo avaliar o desenvolvimento de mudas de pupunheira (Bactris gasipaes H.B.K.) cultivadas em amostras de um Latossolo Vermelho eutroférrico de textura argilosa compactado artificialmente em subsuperfície. As amostras de solo que foram compactadas a quatro níveis de densidade (1,0; 1,13; 1,28 e 1,44kg dm-3) estavam nas camadas intermediárias de 3,5cm de vasos de PVC de 20cm de diametro, que constavam de outras camadas superiores de 15cm e inferiores de 10cm. As avalia es foram realizadas em quatro épocas (4, 6, 8 e 10 meses). O aumento dos níveis de densidade do solo n o influenciou no desenvolvimento da parte aérea das mudas de pupunheira. Independentemente da época estudada, os níveis de densidade do Latossolo argiloso n o impediram a penetra o das raízes da pupunheira.
Assessment of EST-SSR markers for genetic analisys on coffee
Missio, Robson Fernando;Caixeta, Eveline Teixeira;Zambolim, Eunize Maciel;Pena, Guilherme Ferreira;Ribeiro, Ana Paula;Zambolim, Laércio;Pereira, Ant?nio Alves;Sakiyama, Ney Sussumu;
Bragantia , 2009, DOI: 10.1590/S0006-87052009000300003
Abstract: est-ssr markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. seventeen markers, developed from ests (expressed sequence tags) from the brazilian coffee genome project, were used. all markers showed polymorphism among the genotypes assessed. the average number of allele per primer was 5.1. the highest polymorphisms were found within c. canephora (88.2%) and rust-resistant varieties (35.3%). about 29.4% of the markers differentiated c. arabica from híbrido de timor; it was also possible to identify those closest and farthest from c. arabica . the analysis of population-grouped genotypes revealed a 64.0% genetic diversity among and a 36.0% genetic diversity within populations. the differentiation index was 0.637. six markers distinguished four rust-resistance varieties, showing their fingerprinting potential. these results demonstrate the usefulness of est-ssr markers for cross orientation, in diversity and introgression studies, and in genetic mapping.
Satellyptus: analysis and database of microsatellites from ESTs of Eucalyptus
Ceresini, Paulo Cezar;Silva, Cristina Lacerda Soares Petrarolha;Missio, Robson Fernando;Souza, Elaine Costa;Fischer, Carlos Norberto;Guillherme, Ivan Rizzo;Gregorio, Ivo;Silva, Eloiza Helena Tajara da;Cicarelli, Regina Maria Barreto;Silva, Marco Túlio Alves da;Garcia, José Fernando;Avelar, Gustavo Arbex;Porto Neto, Laercio Ribeiro;Mar?on, André Ricardo;Bacci Junior, Maurício;Marini, Danyelle Cristine;
Genetics and Molecular Biology , 2005, DOI: 10.1590/S1415-47572005000400014
Abstract: the main goal of our research was to search for ssrs in the eucalyptus est forests database (using a software for mining ssr-motifs). with this objective, we created a database for cataloging eucalyptus est-derived ssrs, and developed a bioinformatics tool, named satellyptus, for finding and analyzing microsatellites in the eucalyptus est database. the search for microsatellites in the forests database containing 71,115 eucalyptus est sequences (52.09 mb) revealed 20,530 ssrs in 15,621 ests. the ssr abundance detected on the eucalyptus ests database (29% or one microsatellite every four sequences) is considered very high for plants. amongst the categories of ssr motifs, the dimeric (37%) and trimeric ones (33%) predominated. the ag/ct motif was the most frequent (35.15%) followed by the trimeric ccg/cgg (12.81%). from a random sample of 1,217 sequences, 343 microsatellites in 265 ssr-containing sequences were identified. approximately 48% of these ests containing microsatellites were homologous to proteins with known biological function. most of the microsatellites detected in eucalyptus ests were positioned at either the 5? or 3? end. our next priority involves the design of flanking primers for codominant ssr loci, which could lead to the development of a set of microsatellite-based markers suitable for marker-assisted eucalyptus breeding programs.
DISTúRBIOS NUTRICIONAIS INDUZIDOS PELA ADUBA O DE FóSFORO E FERRO EM PLANTAS JOVENS DE GRáPIA (Apuleia leiocarpa)
Evandro Luiz Missio,Fernando Teixeira Nicoloso
Ciência Florestal , 2005,
Abstract: A grápia (Apuleia leiocarpa Vog. Macbride) é uma espécie florestal nativa de grande interesse madeireiro, encontrando-se, atualmente, bastante dizimada. Em trabalhos anteriores, foi observado que as folhas novas de plantas jovens de grápia apresentaram sintomas visuais indicativos de deficiência de Fe na presen a de doses crescentes de P. O presente trabalho teve como objetivo caracterizar os efeitos da intera o da aduba o de P e Fe no balan o nutricional e no crescimento de plantas jovens de grápia cultivadas num ARGISSOLO VERMELHO Distrófico arênico, sob condi es de casa de vegeta o. Os tratamentos consistiram de uma combina o bifatorial (2x3) completa, com dois níveis de aduba o de fósforo (60 e 180 mg kg-1) e três níveis de ferro (0, 6 e 12 mg kg-1); além disso, realizou-se aduba o complementar de N, K, Ca e Mg em todos tratamentos. A análise de crescimento das plantas foi realizada mensalmente, considerando a altura de planta, diametro do caule e número de folhas por planta. Aos 150 dias após a emergência das plantas, avaliaram-se ainda a massa seca de raízes, do caule, das folhas e total da planta, comprimento das raízes, comprimento radicular específico, número de folhas caídas, rela o entre a massa seca de raízes e da parte aérea e a concentra o de P, Fe, Cu e Zn nos tecidos da planta. A rela o de concentra o entre o P e os micronutrientes Fe, Cu e Zn é mais apropriada na avalia o do estado nutricional da grápia que a análise da concentra o absoluta desses elementos nos tecidos. A alta disponibilidade de P no solo causou deficiência de Fe, Cu e Zn nas plantas de grápia. O aumento da disponibilidade de Fe, ocasionado pela aduba o desse elemento, ou pela diminui o do pH do solo, induzida pela aduba o com outros nutrientes, provocou decréscimo nas concentra es de Cu e Zn nas raízes. Na presen a de 180 mg de P kg-1 de solo, a aplica o de 12 mg de Fe kg-1 de solo, na forma de Fe-EDTA, diminuiu a clorose foliar e aumentou o crescimento de plantas jovens de grápia.
Teores de nitrato em folhas de alface em fun o do horário de coleta e do tipo de folha amostrada
Krohn Nádia G.,Missio Robson F.,Ortolan Mara L.,Burin Alcione
Horticultura Brasileira , 2003,
Abstract: Determinou-se os teores de nitrato em fun o dos horários de coleta e avaliou-se o critério de amostragem de folhas, para alface do tipo americana. As plantas foram colhidas na UNIOESTE, Campus de Marechal Candido Rondon, em diferentes horários, às 0; 6; 12 e 18 horas. Após colhidas as folhas foram lavadas em água corrente e destilada, descartando-se as nervuras centrais, sendo separadas da seguinte forma: folhas maturas (externas), folhas recém-maturas (medianas) e folhas jovens (internas). Determinou-se ent o a massa seca e os teores de nitrato. Observaram-se diferen as significativas nos horários de coleta, sendo que durante o dia foram encontrados os menores valores de teores de nitrato e durante a noite os maiores. Em rela o ao critério de amostragem tem-se que as folhas jovens apresentaram maior teor de nitrato, 999 mg de N-NO3-.kg-1 de massa seca, enquanto que as folhas maturas e recém-maturas apresentaram 564 e 468 mg de N-NO3-.kg-1 de massa seca, respectivamente.
Teores de nitrato em folhas de alface em fun??o do horário de coleta e do tipo de folha amostrada
Krohn, Nádia G.;Missio, Robson F.;Ortolan, Mara L.;Burin, Alcione;Steinmacher, Douglas A.;Lopes, Mário César;
Horticultura Brasileira , 2003, DOI: 10.1590/S0102-05362003000200019
Abstract: the nitrate levels in function of the harvest times, and the sampling criterion of american type lettuce leaves were determined. the plants were harvested at unioeste in marechal candido rondon, paraná state, brazil, at 0 o'clock, 6 a.m., 12 o'clock and 6 p.m. in sequence, the leaves were washed, discarding the central nervure, later they were separated according to mature leaves (external leaves), recently mature leaves (median leaves) and new leaves (internal leaves). the dry weight and the nitrate level were determined. leaves harvested during the day presented lower values of nitrate levels in comparison to leaves harvested during the night. about the sampling criterion the nitrate levels were superior in new leaves, 999 mg of n-no3-.kg-1 dry weight, while the mature leaves and the recently matured leaves accumulated 564 and 468 mg of n-no3-.kg-1 dry weight, respectively.
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