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Search Results: 1 - 10 of 22809 matches for " Roberto; Fari?as "
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Identificación de cepas de Aeromonas de origen clínico con perfiles fenotípicos atípicos
Laura Bravo Fari?as,Margarita Ramírez,Roberto Cabrera,Paolo Guglielmetti
Revista Cubana de Medicina Tropical , 1999,
Abstract: Un total de 47 cepas de Aeromonas aisladas de pacientes con gastroenteritis fue analizado para 40 caracteres fenotípicos y para la evaluación de la taxonomía numérica basada en 27 pruebas discriminatorias. Se evidenció que los aislamientos clínicos mostraron una relativa distancia fenotípica y los grupos de cepas que mostraron perfiles atípicos fueron comparados con las especies tipos, mediante los actuales esquemas de identificación. A total of 47 strains of Aereomonas isolated from patients with gastroenteritis was analized for 40 phenotypical characters and for evaluating the numeric taxonomy based on 27 discriminatory tests. It was proved that the clinical isolates showed a relative phenotypical distance and the groups of strains that had atypical profiles were compared with the type species by the present identification schemes.
Gel del Vimang con actividad antifotoenvejecimiento: estudio histopatológico preliminar
Concepción,Angel R.; de la Pe?a,Roberto; Fari?as,Lucía;
Revista Cubana de Investigaciones Biom??dicas , 2003,
Abstract: studies of a new formulation (vimang's gel) were conducted to evaluate, in this case, histopathological parameters of a model in vivo, starting from the antecedent that, according to results in vitro, vimang has antioxidant properties.
Caracterización de un brote de Shigella boydii 14 por primera vez en Cuba
Margarita Ramírez álvarez,Laura Bravo Fari?as,Alina Llop Hernández,Roberto Cabrera Ortega
Revista Cubana de Higiene y Epidemiología , 1999,
Abstract: Se propuso la caracterización de 14 cepas de Shigella boydii 14 aisladas de pacientes con enfermedad diarreica aguda mediante sus plásmidos de resistencia y de las proteínas de la membrana externa presentes en ellas. Se realizó la determinación de la susceptibilidad antimicrobiana por el método de concentración mínima inhibitoria, la extracción de plásmidos R fue según Manaitis, los extractos proteicos de las cepas se obtuvieron según el método de Blaser modificado y las proteínas de la membrana externa fueron separadas por SDS-PAGE por el método de Laemmli. Se comprobó que las cepas resultaron resistentes a la ampicilina (100 %), la tetraciclina (70 %) y al cotrimoxazol (50 %), y sensibles al ácido nalidíxico y a la ciprofloxacina. Se observó la presencia de plásmidos al nivel de los 43; 23; 20; 5,6 y 1,2 kb. Las proteínas de la membrana externa y el perfil proteico demostraron diferencias con otras especies de Shigella. Este serotipo de Shigella se aísla por primera vez en Cuba y y sus características la hacen altamente patógena y de muy difícil diagnóstico, por lo que la caracterización de este brote es importante desde el punto de vista epidemiológico. The characterization of strains of Shigella boydii isolated from patients with acute diarrheal disease by resistance susplasmides, and of the proteins of the outer membrane that appear in these strans was suggested. The antimicrobial susceptibility was determined by the minimum inhibitory concentration method, and the extraction of plasmids R. According to Manaitis, the protein extracts of the strains were obtained by the modified method of Blaser, whereas the proteins of the outer membrane were separated by SDS-PAGE, using the Laemmli's method. It was proved that the strains were resistant to ampicillin (100 %), tetracycline (70 %) and cotrimoxazole (50 %), and sensitive to nolidixic acid and to cyprofoflaxine. Plasmides were observed at the level of 43; 23; 20; 5.6 and 1.2 kb. The proteins of the outer membrane and the protein profile proved to be different from other species of Shigella. This serotype of Shigella was isolated for the first time in Cuba and its characteristics make it highly pathogen and very difficult to be diagnosed. That's why, the characterization of this outbreak is important from the epidemiological point of view.
Ensayo clínico de reto, para evaluar una cepa candidata a vacuna contra el cólera Challenge clinical trial for evaluation of a vaccine candidate strain against cholera
Manuel Díaz Jidy,Antonio Pérez Rodríguez,Roberto Fernández Llanes,Laura Bravo Fari?as
Revista Cubana de Medicina Tropical , 2010,
Abstract: INTRODUCCIóN: la cepa atenuada 638 Vibrio cholerae O1 El Tor, Ogawa, ha demostrado ser bien tolerada e inmunogénica por vía oral en estudios realizados en voluntarios sanos. OBJETIVO: se evaluó la protección conferida contra el cólera, en un ensayo clínico de reto, para el escalado tecnológico y farmacéutico de este candidato vacunal como ingrediente activo a escala industrial. MéTODOS: en el estudio participaron 21 voluntarios sanos, 12 de ellos recibieron el candidato vacunal, y 9 ingirieron un placebo; 28 d después, todos recibieron una dosis infectante de una cepa virulenta de V.cholerae. RESULTADOS: la diarrea se registró en 7 de los 9 placebos, mientras que ninguno de los voluntarios vacunados presentó diarrea. Los voluntarios placebos del grupo sanguíneo O, tuvieron diarreas con mayor frecuencia e intensidad. Todos los voluntarios en el grupo placebo excretaron V. cholerae mientras que solo 3 (25 %) de los 12 vacunados la excretaron. CONCLUSIONES: en este modelo de ensayo de reto, la cepa 638 demostró proteger contra la diarrea producida por una cepa virulenta de V. cholerae. INTRODUCTION: live attenuated oral Vibrio cholerae O1 El Tor, Ogawa strain 638 has demonstrated to be well tolerated and immunogenic when administrated orally in studies carried out in healthy volunteers. OBJECTIVE: to evaluate the protection against cholera infection in a challenge clinical trial, for the technological and pharmaceutical scale-up of this vaccinal candidate as active ingredient at industrial level. METHOD: a total of 21 healthy volunteers were involved in this trial; the vaccine candidate was administered to 12 of them and the remaining nine were given the placebo. Twenty eight days later, all of them received an infective dose of a V. cholerae virulent strain. RESULTS: diarrheas were observed in 7 out of 9 placebos whereas not a single vaccinated volunteer showed diarrheas. More frequent and intense loose stools were found in the placebo volunteers with O-blood group. All volunteers in he placebo group excreted V. cholerae, but only three (25%) out of the 12 vaccinated volunteers did so. CONCLUSION: in this challenge clinical trial model, the 638 strain proved to protect people against the diarrhea caused by a virulent V. cholerae strain.
Gel del Vimang con actividad antifotoenvejecimiento: estudio histopatológico preliminar
Angel R. Concepción,Roberto de la Pe?a,Lucía Fari?as
Revista Cubana de Investigaciones Biom??dicas , 2003,
Abstract: Se realizaron estudios de una nueva formulación (gel del Vimang), para evaluar en este caso parámetros histopatológicos, de un modelo in vivo; partiendo del antecedente que por resultados in vitro se sabe que el Vimang posee propiedades antioxidantes. Studies of a new formulation (Vimang's gel) were conducted to evaluate, in this case, histopathological parameters of a model in vivo, starting from the antecedent that, according to results in vitro, Vimang has antioxidant properties.
Results on prognostic value of mutations in localized gastrointestinal stromal tumors (GIST): in one single center Valor pronóstico de las mutaciones en tumores estromales gastrointestinales localizados (GIST): resultados de un solo centro
Marina Garcés-Albir,Roberto Marti-Obiol,Fernando López-Mozos,Silvia Calabuig-Fari?as
Revista Espa?ola de Enfermedades Digestivas , 2012,
Abstract: Introduction: to study the prognostic value of mutations in KIT or PDGFRA in gastrointestinal stromal tumors (GIST) managed in our department. Materials and methods: forty five patients with localized GIST underwent surgery between 1998 and 2010. Thirty six patients were enrolled in a retrospective study. DNA was isolated from 3 to 5 μm sections of fixed and paraffin-embedded tissue. Exon 9, 11, 13 and 17 of c-kit gene and exon 12 and 18 of PDGFRA were amplified by PCR and sequenced. Results: tumors with mutations were larger at the surgery and showed higher mitotic count (p < 0.05). The mutations were found in 22 patients (61.2%), 18 had mutations in exon 11 of c-kit gene. PDGFRA mutations were located in exon 12. The 5-years relapse-free survival rate for patients with tumors having mutations was 38% and 100% for patients without mutations (p < 0.01). The 5-year survival rate was significantly worse for patients with mutations (20 vs. 97%, p < 0.01), with tumors larger than 5 cm (28 vs. 97%, p < 0.01) and with > 50 mitosis/HPF (42 vs. 88%, p < 0.03). Multivariate analyses indicated that the mutations, mitotic counts, and tumor size were independent prognostic factors for survival in patients with localized GIST. Conclusions: in this series, having a detected mutation is a poor prognostic factor with significantly increased recurrence rate and shortens survival.
Caracterización de un brote de Shigella boydii 14 por primera vez en Cuba
Ramírez álvarez,Margarita; Bravo Fari?as,Laura; Llop Hernández,Alina; Cabrera Ortega,Roberto; García Rodríguez,Belkys; Fernández Abreu,Anabel;
Revista Cubana de Higiene y Epidemiolog?-a , 1999,
Abstract: the characterization of strains of shigella boydii isolated from patients with acute diarrheal disease by resistance susplasmides, and of the proteins of the outer membrane that appear in these strans was suggested. the antimicrobial susceptibility was determined by the minimum inhibitory concentration method, and the extraction of plasmids r. according to manaitis, the protein extracts of the strains were obtained by the modified method of blaser, whereas the proteins of the outer membrane were separated by sds-page, using the laemmli's method. it was proved that the strains were resistant to ampicillin (100 %), tetracycline (70 %) and cotrimoxazole (50 %), and sensitive to nolidixic acid and to cyprofoflaxine. plasmides were observed at the level of 43; 23; 20; 5.6 and 1.2 kb. the proteins of the outer membrane and the protein profile proved to be different from other species of shigella. this serotype of shigella was isolated for the first time in cuba and its characteristics make it highly pathogen and very difficult to be diagnosed. that's why, the characterization of this outbreak is important from the epidemiological point of view.
Análisis de los temas de terminación de residencia y maestría del curso 1995-1996
Fari?as Reinoso,Ana Teresa;
Educaci?3n M??dica Superior , 1997,
Abstract: a documental review of the works done for the completion of residencies such as doctoral dissertations and master degress during 1995-1996 at the faculty of public health, was performed. the main type of investigation carried out was found to be the research of descriptive studies. it was evidenced that the main priorities are the tutorship of thesis done by professors of lower teaching categories. it was observed as a positive element, the fact that more than 50 % of the investigations undertaken provided solutions to the problems concerning the service throught research techniques within the health service.
La ense?anza de la vigilancia en salud
Fari?as Reinoso,Ana Teresa;
Educaci?3n M??dica Superior , 2005,
Abstract: the way by which the contents of health surveillance were taught at the different curricula aimed at epidemiologists, hygienists, biostaticians, family physicians and public health and primary care masters in cuba was described. the documentary research allowed us to observe that in the first stage, the health surveillance was taught in isolated topics in epidemiology subject. afterwards, it became an academic subject that emphasized acquisition of theoretical knowledge, and finally it was systematized as a discipline in which the student summarizes "what he/she really knows and the way of doing it" so as to bring that student closer to the realities of surveillance in the national health care system
El lecho de Procusto o la convención sobre la competencia humana
León, Gloria Fari?as;
Psicologia Escolar e Educacional , 2011, DOI: 10.1590/S1413-85572011000200016
Abstract: the issue of competences has won today a major forum for discussion on human development. the theme was reissued by d. mc clelland in the psychology of the seventy, with new meanings and uses that not always conform to the requirements of development of psychology as a science and profession. neither it conforms to the demands of advanced education, one of the largest fields of application of concept.in this paper we analyse the issue, attempting to clarify theoretical and practical implications of the concept, from a critical standpoint. we found different arguments, about the the term competence: on the hand, it has a conventional content due to instrumental and utilitarian purposes, and on the other hand, its use delays the development of psychology as a science and profession. however, the concept can be used in certain circumstances, which are also clarified. the sources we use for analysis were the author's observations and the published literatura in the area, leading to a historical and cultural study of the concept
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