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Search Results: 1 - 10 of 24927 matches for " Roberto Espinosa "
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Prevalencia de displasia de cuello uterino en pacientes portadoras de virus papiloma humano, Chile
Andrea Verdessi,Francisco Perán,Roberto Espinosa
CIMEL. Ciencia e Investigación Médica Estudiantil Latinoamericana , 2006,
Abstract: Introducción: Los programas de tamizaje para prevención de cáncer cérvicouterino han reducido su incidencia y mortalidad significativamente, siendo el método más utilizado mundialmente la citología cervical o Papanicolau. Además existen otros métodos con mayor sensibilidad y especificidad, pero de mayor costo. Entre éstos existe la Captura Híbrida, que permite tipificación en cepas virales del Virus Papiloma Humano según su potencial oncogénico. El objetivo de este estudio fue describir la prevalencia de displasia cervical en pacientes infectadas con Virus Papiloma Humano, su distribución por edad y potencial oncogénico de la cepa viral.Materiales y métodos: Fueron utilizados 274 resultados de biopsias de cuello uterino con diagnóstico histopatológico de infección por Virus Papiloma Humano entre enero de 2003 y septiembre de 2005, del Servicio de Anatomía Patológica del Hospital Naval Almirante Nef de Vi a del Mar. Se registraron nombre, edad, diagnóstico histopatológico, disponibilidad de captura híbrida y sus resultados.Resultados: La prevalencia observada de displasias cervicales fue de 45,25%. La distribución de los grados de displasia fueron: Lesión Intraepitelial I 48,28%, Lesión Intraepitelial II 27,8% y Lesión Intraepitelial III 23,76%. La prevalencia según edad presenta su valor más alto entre los 15 y 29 a os. Conclusiones: La prevalencia de displasia cervical fue de 45,25%, se observa una mayor frecuencia de biopsias sin Lesión Intraepitelial en el grupo portador de Virus Papiloma Humano de alto riesgo oncogénico, por lo que sugerimos utilizar a la Captura Híbrida como complemento al programa de tamizaje, para una mejor cobertura de pacientes de mayor riesgo.
Nitrogen Source Affects Glycolipid Production and Lipid Accumulation in the Phytopathogen Fungus Ustilago maydis  [PDF]
Ariana Zavala-Moreno, Roberto Arreguin-Espinosa, Juan Pablo Pardo, Lucero Romero-Aguilar, Guadalupe Guerra-Sánchez
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.413104
Abstract: When cultured in medium limited of nitrogen sources, the phytopathogen Ustilago maydis produces two amphipathic glycolipids: Ustilagic acid (UA) and Mannosylerythritol lipid (MEL), which in addition to the hydrophilic moiety, contain dior tri-hydroxylated C16 fatty acids (UA), or C8 and C16 saturated fatty acids (MEL). We compared the growth and morphology of cells in YPD and in minimum media containing glucose and nitrogen sources such as nitrate or urea and those deprived of nitrogen. Nitrogen-starved cells showed a dramatic accumulation of internal lipids identified as lipid droplets when stained with the hydrophobic probe BODIPY; these lipid droplets were enriched in unsaturated fatty acids. Fatty acids in YPD or medium containing nitrate as nitrogen source showed a combination of saturated/unsaturated lipids, but when urea was the nitrogen source, cells only contained saturated fatty acids. The glycolipid profiles produced in the presence or absence of nitrogen showed preferences towards the production of one kind of glycolipid: cells in media containing nitrate or urea produced different proportions of UA/MEL, but under nitrogen starvation cells contained only UA. The emulsification capacity of the glycolipids produced in media with or without nitrogen was similar (72% - 76%). HPLC of the glycolipids allowed the separation of fractions with different emulsifying characteristics. Our results indicate that U. maydis accumulates lipid droplets when deprived of nitrogen source and confirm that UA is not under nitrogen control, but rather that MEL and lipid droplets are produced and oppositely regulated by nitrogen.
Enfermedad de Erdheim-Chester. Una causa rara de derrame pericárdico: Caso clínico Erdhei-Chester disease: Report of one case
Jorge Vega,Marcela Cisternas,Michel Bergoeing,Roberto Espinosa
Revista médica de Chile , 2011,
Abstract: We report a 76-year-old male who was admitted due to progressive congestive heart failure lasting several months. An echocardiogram showed a large pericardial effusion with early signs of pericardial tamponade and an irregular surface suggestive of cancer infiltration. The patient was operated, creating a pericardial window and draining 1,200 ml of a brownish yellow fluid with abundant cellularity. Pericardial biopsy showed infiltration by CD68 (+), CD1a (-) and S100 (-) cells. Twenty-eight months earlier, due to fatigue, dyspnea, and a non-specific inflammatory process, an enhanced-contrast-scan showed that aorta was coated with a hypodense tissue that began near the aortic valve and extended until the inferior mesenteric artery, with stenosis of the left subclavian, celiac axis, renal and upper mesenteric arteries. An angioplasty and stent placing was carried out in the last two arteries. Both kidneys had the appearance of "hairy kidneys". A bone scan showed increased uptake in femurs and tibiae and X-ray examination showed osteosclerosis in metaphysis and diaphysis. The diagnosis of Erdheim-Chester disease (non-Langerhans-cell histiocytosis) was made and the patient was treated with steroids and methotrexate.
Fluid elasticity increases the locomotion of flexible swimmers
Julian Espinosa-Garcia,Eric Lauga,Roberto Zenit
Physics , 2013, DOI: 10.1063/1.4795166
Abstract: We conduct experiments with flexible swimmers to address the impact of fluid viscoelasticity on their locomotion. The swimmers are composed of a magnetic head actuated in rotation by a frequency-controlled magnetic field and a flexible tail whose deformation leads to forward propulsion. We consider both viscous Newtonian and glucose-based Boger fluids with similar viscosities. We find that the elasticity of the fluid systematically enhances the locomotion speed of the swimmer, and that this enhancement increases with Deborah number. Using Particle Image Velocimetry to visualize the flow field, we find a significant difference in the amount of shear between the rear and leading parts of the swimmer head. We conjecture that viscoelastic normal stresses lead to a net elastic forces in the swimming direction and thus a faster swimming speed.
Purification and Biochemical Characterization of a Protease Inhibitor II Family from Jalapeño Pepper (Capsicum annuum L.)  [PDF]
Juan Pablo Carrillo-Montes, Roberto Arreguín-Espinosa, José Luis Mu?oz-Sánchez, Manuel Soriano-García
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.57078
Abstract:

Capsicum annuum L. was initially domesticated in Mexico and northern Central America, and represented an ancient Neotropical plant food complex. The purpose of this paper is to report the isolation and purification of a novo-member of a protease inhibitor from jalapeño pepper (Capsicum annuum L.) (PIJP). The molecular weight of PIJP inhibitor is 5.95 kDa with 56 amino acids and 6 Cys residues with high inhibitory activity to trypsin with a Ki value of 95 nM. This inhibitor according to the alignment with homologous from NCBI and Pfam databases is a member of proteinase inhibitors II. It is worthwhile to mention a major compositional difference between the proteinase inhibitor II families which have 8 Cys residues. PIJP is the first purified proteinase inhibitor, member of this family with only 6 Cys residues.

Briquetagem da granalha de a?o recuperada do resíduo de rochas ornamentais
Junca, Eduardo;Oliveira, José Roberto de;Espinosa, Denise Crocce Romano;Tenório, Jorge Alberto Soares;
Rem: Revista Escola de Minas , 2011, DOI: 10.1590/S0370-44672011000200007
Abstract: this paper presents the results obtained with the production of briquettes from the steel grit found in the residue of ornamental rocks. the grit recovered by magnetic separation was characterized by titrimetric analysis, eds (electron dispersive spectroscopy) and x-ray diffraction for the analysis of iron concentration in the residue. the size and distribution of particles were obtained by the granulometric analysis method and scanning electron microscopy (sem). the process resulted in a concentrate containing 93% metallic iron. the maximum load before fracture of the green briquettes was 1.02kn and of the dry briquettes was 3.59kn.
Infec??es genitais em mulheres atendidas em Unidade Básica de Saúde: prevalência e fatores de risco
Barcelos, Mara Rejane Barroso;Vargas, Paulo Roberto Mer?on de;Baroni, Carla;Miranda, Angélica Espinosa;
Revista Brasileira de Ginecologia e Obstetrícia , 2008, DOI: 10.1590/S0100-72032008000700005
Abstract: purpose: to describe the prevalence and behavioral profile of genital infections in women attended at a primary health unit in vitoria, es. methods: a transversal study including 14 to 49-year-old women attended by the family health program (fhp). exclusion criteria were: having been submitted to gynecological examination in less than one year before, and history of recent treatment (in the last three months) for genital infections. an interview including socio-demographic, clinical and behavioral data was applied. genital specimens were collected for cytology, gram bacterioscopy and culture, and urine sample for molecular biological test for chlamydia trachomatis. results: two hundred and ninety-nine women took part in the study. the median age was 30.0 (interquartile interval: 24;38) years old; the average age of the first intercourse was 17.3 (sd=3.6) years old. the first pregnancy average age was 19.2 (3.9) years old. about 70% reported up to 8 years of schooling; 5% reported previous sexually transmitted diseases (std), and 8%, the use of illicit drugs. only 23.7% reported consistent use of condoms. clinical complaints were: genital ulcer (3%); dysuria (7.7%); vaginal discharge (46.6%): pruritus (20%) and pelvic pain (18%). prevalence rates were: chlamydia trachomatis 7.4%; gonorrhea 2%; trichomoniasis 2%; bacterial vaginosis 21.3%; candidiasis 9.3%; and cytological changes suggestive of hpv 3.3%. in the final logistic regression model, the factors independently associated to genital infections were: abnormal cervical mucus, or=9.7 (ci95%=5.6-13.7), previous hiv testing, or=6.5 (ci95%=4.0-8.9), having more than one partner during the previous year, or=3.9 (ci95%=2.7-5.0), and having more than one partner in life, or=4.7 (ci95%=2.4-6.8). conclusions: results show a high rate of genital infections and the need of preventive measures, such as std surveys and risk reduction programs for women that look for routine gynecological service.
Gasto de agua de limpieza y tratamiento del residual en naves de ceba porcina
Chao Espinosa,amón; Sosas Caceres,Roberto; Díaz Capdesu?er,Yasser;
Revista Ciencias T??cnicas Agropecuarias , 2012,
Abstract: a study on cleaning pens system was carry out in three pig housing from one 80 sows pig farm of swine research institute, as well as wastewater treatment thru 90 m3 fixed dome digester. that study was carry out during 3 months in conditions of production. the next measures was taken: animal number and weigh; time of cleaning floor, quantity of used water and total amount of wastewater (excreta, urine and used water for cleaning). in each pig housing were: 91; 87 and 111 animals, their average weigh was 52; 56 and 72 kg respectable. the time for pens cleaning was 39;45 and 55 min. the quantity of used water was 2,28; 2,31 and 2,30 m3 and the amount of wastewater (including excreta and urine) was 2,55, 2,57 and 3,24 m3. for all theses measures was made a varianza test. the total amount of wastewater for the tree pig housing were 8,36 m3 and the used of water were 25,51 l per pig. the remotion of total solids, volatils solids and chemical oxigen demand were 75;79 and 62 respectibily. these results are inferiors than the others obteneid in some evaluations in diferents fixed dome digesters, there is appreciable a lot of water used in cleanming the pens, therefor it is needed a considerable volumen wastewater system and the necessary increase of material and finance of inversion.
Saúde sexual e reprodutiva em penitenciária feminina, Espírito Santo, Brasil
Miranda,Angélica Espinosa; Mer?on-de-Vargas,Paulo Roberto; Viana,Maria Carmen;
Revista de Saúde Pública , 2004, DOI: 10.1590/S0034-89102004000200015
Abstract: objective: to describe the sociodemographic profile and health problems of inmates in a women's prison. methods: a descriptive study was conducted from march to september 1997 in a women's prison in the state of espírito santo, brazil. all inmates were invited to participate in the study. a total of 121 women aged more than 18 years were interviewed. a structured questionnaire was used to collect data on sociodemographics, clinical and criminal past history. following the interview, subjects underwent clinical and gynecological examination. results: of 121 women included in the study, the mean age was 30.2 years (sd 8.98) and the mean of schooling was 4.8 years (sd 3.50). all participants reported previous sexual activity, the mean age of the first sexual intercourse was 15.2 years (sd 2.55) ranging from 9 to 27 years. previous stds were reported by 28%; 12 (9.9%) were pregnant at the time. teenage pregnancy was often reported. most women reported no use of either any contraceptive methods or condoms. tubal ligation was seen in 19.8% and abnormal pap smear in 26.9%. conclusions: knowledge about health problems inside the prison system can contribute to promoting and increasing social rehabilitation. however, effective results could be seen only with collaboration between public health authorities and the prison system.
Enfermedad de Erdheim-Chester. Una causa rara de derrame pericárdico: Caso clínico
Vega,Jorge; Cisternas,Marcela; Bergoeing,Michel; Espinosa,Roberto; Zapico,Alvaro; Chadid,Pedro; Santamarina,Mario;
Revista médica de Chile , 2011, DOI: 10.4067/S0034-98872011000800011
Abstract: we report a 76-year-old male who was admitted due to progressive congestive heart failure lasting several months. an echocardiogram showed a large pericardial effusion with early signs of pericardial tamponade and an irregular surface suggestive of cancer infiltration. the patient was operated, creating a pericardial window and draining 1,200 ml of a brownish yellow fluid with abundant cellularity. pericardial biopsy showed infiltration by cd68 (+), cd1a (-) and s100 (-) cells. twenty-eight months earlier, due to fatigue, dyspnea, and a non-specific inflammatory process, an enhanced-contrast-scan showed that aorta was coated with a hypodense tissue that began near the aortic valve and extended until the inferior mesenteric artery, with stenosis of the left subclavian, celiac axis, renal and upper mesenteric arteries. an angioplasty and stent placing was carried out in the last two arteries. both kidneys had the appearance of "hairy kidneys". a bone scan showed increased uptake in femurs and tibiae and x-ray examination showed osteosclerosis in metaphysis and diaphysis. the diagnosis of erdheim-chester disease (non-langerhans-cell histiocytosis) was made and the patient was treated with steroids and methotrexate.
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